森永生科学研究所株式会社,Wheat/Gluten (Gliadin) ELISA KitⅡ Cat. M2114,Morinaga


Food Allergen ELISA KitⅡ

  • Products
  • Food Allergen ELISA KitⅡ
  • Wheat/Gluten (Gliadin) ELISA KitⅡ Cat. M2114

Wheat/Gluten (Gliadin) ELISA KitⅡ
Cat. M2114

Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114

Wheat/Gluten (Gliadin) ELISA KitⅡ is sandwich enzyme immunoassay for the quantitative determination of wheat protein or gluten in processed or unprocessed foods.

Our original extraction solution enables target protein to solubilize even though it was insolubilized by food processing.

The extraction solution is identical for all of our ELISA KitⅡ.

An extraction procedure is simple and also the same as all ELISA KitⅡ.

  • Specifications
  • Food reactivity data
  • Documents
  • Instructional video
  • Technical note

Specifications

Test Method ELISA
No. wells 96 wells / kit
Limit of detection 0.31 ppm [ µg of wheat protein / g of food ] / 0.26 ppm [ µg of gluten / g of food ]
Limit of quantification 0.31 ppm [ µg of wheat protein / g of food ] / 0.26 ppm [ µg of gluten / g of food ]
Target antigen Gliadin
Calculated result Wheat protein or Gluten
Range of assay 0.31 – 20 ppm for wheat protein, 026 – 17 ppm for gluten
Total reaction time 1 h 50 min
Storage temperature 2℃ – 8℃

Food reactivity data

Please check the lot number of the kit and refer to the file below corresponding to the last three digits of the lot number.

  • #001 – 008
  • #009 – 039
  • #040 – 041
  • #042 – 054
  • #055 – 059
  • #060 – 063
  • #064 – 068
  • #069 –

Documents

  • SDS
  • Kit insert
  • Leaflet

Instructional video

  • Learn how to use Food Allergen ELISA KitⅡ

Technical note

– Why does our test kit can detect target proteins from both raw materials and processed foods?

Processed food contains a mix of native and denatured proteins
Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114
Denatured proteins are insoluble and difficult to be detected by using popular buffer to extract
Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114
The Morinaga kit detects both native and denatured target proteins, making it ideal for both raw materials and processed foods

Our original extraction buffer enables to solubilize denatured proteins.

It also allows native proteins to be a same conformation.

Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114

Contact us

Related product

Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114Rapid Test ProⅡ for Gluten
Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114Rapid Test Easy for Gluten
Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114QC Materials
Wheat/Gluten (Gliadin) ELISA KitⅡ  Cat. M2114Reagent A, B, C

森永生科学研究所株式会社,Wheat/Gluten ELISA Kit Cat. M2103,Morinaga


AOAC PTM approved test kit for gluten analysis

  • Products
  • Wheat/Gluten ELISA Kit Cat. M2103

Wheat/Gluten ELISA Kit
Cat. M2103

Wheat/Gluten ELISA Kit  Cat. M2103

Wheat/Gluten ELISA Kit is sandwich enzyme immunoassay for the quantitative determination of gluten amount in raw materials and processed or non-processed foods.

The test kit is approved by AOAC PTM (performance tested method).

In addition, the test kit is listed on the GFCO (Gluten-Free Certification Organization) test kits.

  • Specifications
  • Food reactivity data
  • Documents
  • Technical note

Specifications

Test Method ELISA
No. wells 96 wells / kit
Limit of detection 0.31 ppm [ µg of wheat protein / g of food ] / 0.26 ppm [ µg of gluten / g of food ]
Limit of quantification 0.31 ppm [ µg of wheat protein / g of food ] / 0.26 ppm [ µg of gluten / g of food ]
Target antigen Gliadin
Calculated result Wheat protein or Gluten
Range of assay 0.31 – 20 ppm for wheat protein, 026 – 17 ppm for gluten
Assay time 2 h
Storage temperature 2℃ – 8℃

Food reactivity data

Please check the lot number of the kit and refer to the file below corresponding to the last three digits of the lot number.

  • #096 – 105
  • #106 – 124
  • #125 – 129
  • #130 – 136
  • #137 – 146
  • #147 –

Documents

  • SDS
  • Kit insert
  • Leaflet
  • AOAC PTM Certification

Technical note

– Why does our test kit can detect target proteins from both raw materials and processed foods?

Processed food contains a mix of native and denatured proteins
Wheat/Gluten ELISA Kit  Cat. M2103
Denatured proteins are insoluble and difficult to be detected by using popular buffer to extract
Wheat/Gluten ELISA Kit  Cat. M2103
The Morinaga kit detects both native and denatured target proteins, making it ideal for both raw materials and processed foods

Our original extraction buffer enables to solubilize denatured proteins.

It also allows native proteins to be a same conformation.

Wheat/Gluten ELISA Kit  Cat. M2103

Contact us

Related product

Wheat/Gluten ELISA Kit  Cat. M2103Wheat/Gluten (Gliadin) ELISA KitⅡ
Wheat/Gluten ELISA Kit  Cat. M2103Rapid Test ProⅡ for Gluten
Wheat/Gluten ELISA Kit  Cat. M2103Rapid Test Easy for Gluten
Wheat/Gluten ELISA Kit  Cat. M2103QC Materials

森永生科学研究所株式会社,Wheat/Gluten ELISA Kit

Wheat/Gluten ELISA Kit

Wheat/Gluten ELISA Kit

Wheat/Gluten ELISA Kit

キットの特長

・米国FDAより性能を評価した方法として紹介された高性能なグルテン測定キットです。また、グルテンフリー表示の確認法としてFDAの検査で使用されています。

・国際的認証機関AOACのPTM認証を取得しています(Certificate No. 011804)。

・国際的に著名なグルテンフリー認証組織(GFCO;https://gfco.org/)のグルテン分析検査法として認定されています。

・可溶化効果の高いオリジナル抽出液を用いることにより、食品の加工度によらずグルテンを定量することができます。

取扱説明書・SDS

取扱説明書・SDS
製品コード 製品名 操作手順書
(日本語)
取扱説明書
(英語)
SDS
M2103 Wheat/Gluten ELISA Kit
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キットの構成

品名 容量 数量
A Antibody-coated Microplate Module 8ウェル×6本 2パック
B Wheat Standard (50ng/mL) 1 mL 2本
C Enzyme-conjugated Antibody 13 mL 1本
D Enzyme Substrate (TMB Solution) 13 mL 1本
E Stop Solution (1N Sulfuric Acid) 13 mL 1本
F Sample Buffer (20X Concentrate) 50 mL 1本
G Wash Buffer (20X Concentrate) 50 mL 1本
H Extraction Component A (20X Concentrate) 55 mL 1本
Frame for mounting the microplate module 1個
Microplate cover 1枚

その他必要な器具・装置

食品検査の場合

  • 2-メルカプトエタノール
  • 蒸留水(または脱イオン水)
  • マイクロピペット(20〜1000μL)、チップ
  • メスシリンダー
  • ポリプロピレン製遠心管(50 mL)
  • ポリプロピレン製チューブ(1〜2 mL)
  • チューブラック
  • pH試験紙
  • ホモジナイザー/ブレンダー
  • 水浴(沸騰を維持できるもの)もしくは振とう機
  • 遠心分離機
  • ボルテックスミキサー
  • アスピレーターまたはマイクロプレート洗浄装置
  • マイクロプレートリーダー(主波長 450nm・副波長 600〜650nm)

注文書

注文書はこちらです。

当社直接販売です。FAXでご注文ください。