Universal Plus mRNA-Seq 0520-A01

货号	品名	规格
0508-A01	Universal Plus mRNA-Seq	96
0520-24	Universal Plus mRNA-Seq with NuQuant	24
0520-A01	Universal Plus mRNA-Seq with NuQuant	A01
0520B-A01	Universal Plus mRNA-Seq with NuQuant	96
0521-24	Universal Plus mRNA-Seq with NuQuant, Human Globin AnyDeplete + Dual Unique Index	24
0521-A01	Universal Plus mRNA-Seq with NuQuant, Human Globin AnyDeplete + Dual Unique Index	A01
0521B-A01	Universal Plus mRNA-Seq with NuQuant, Human Globin AnyDeplete + Dual Unique Index	A01
9147-A01	Universal Plus mRNA-Seq , Human Globin AnyDeplete + Dual Unique Index	A01
0522-24	Universal Plus mRNA-Seq with NuQuant, Custom AnyDeplete + Dual Unique Index	24
0522-A01	Universal Plus mRNA-Seq with NuQuant, Custom AnyDeplete + Dual Unique Index	A01
0522B-A01	Universal Plus mRNA-Seq with NuQuant, Custom AnyDeplete + Dual Unique Index	96
0408-A01	mRNA Selection Module	A01
0359-A01	ANyDeplete Module	A01

试剂盒特点：

1. 样本起始量10ng-1ug total RNA起始；

2. 192个dual index避免index hopping；

3. 无接头二聚体污染，无需接头稀释；

4. NuQuant技术无需qPCR，使用qubit既可定量；

5. 可以做常规mRNA建库，也可以选择性去除线粒体及血球蛋白mRNA。

试剂盒文献：

1. 12-h clock regulation of genetic information flow by XBP1s

1 January, 2020

Pages: e3000580 doi: 10.1371/journal.pbio.3000580

2. 12h-clock control of central dogma information flow by XBP1s

doi: 10.1101/559039v1.abstract

3．Acute Lengthening of Progenitor Mitosis Influences Progeny Fate during Cortical Development in vivo

15 June, 2020

Pages: 1-18 doi: 10.1159/000507113

4. ADAR1 mediated regulation of neural crest derived melanocytes and Schwann cell development

Pages: 198 doi: 10.1038/s41467-019-14090-5

5. Adenosinergic Signaling Alters Natural Killer Cell Functional Responses

Pages: 2533 doi: 10.3389/fimmu.2018.02533

6. Ancestral Folate Promotes Neuronal Regeneration in Serial Generations of Progeny

doi: 10.1007/s12035-019-01812-5

7. Best foot forward: Nanopore long reads, hybrid meta-assembly and haplotig purging optimises the first genome assembly for the Southern Hemisphere blacklip abalone (Haliotis rubra)

doi: 10.3389/fgene.2019.00889

8. Best Foot Forward: Nanopore Long Reads, Hybrid Meta-Assembly, and Haplotig Purging Optimizes the First Genome Assembly for the Southern Hemisphere Blacklip Abalone (Haliotis rubra)

doi: 10.3389/fgene.2019.00889

9. Brain-Mimetic Hydrogel Platform for Investigation of Glioblastoma Drug Resistance

10. Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora

doi: 10.1038/s41598-020-67097-0

金畔生物试剂,5min Universal Ligation Mix

5min Universal Ligation Mix

快速的酶连克隆试剂盒

5min Universal Ligation Mix

兼容：兼容黏性末端，平滑末端，TA连接，Linker或Adapter连接；

快速：25℃，5 min即可快速连接；

高效：克隆效率高。

图1. 5 min Universal Ligation Mix实验流程示意图

5min Universal Ligation Mix

5 min Universal Ligation Mix是一种酶和Buffer混合的即用型2 × 预混液，内含T4 DNA ligase，催化双链DNA或RNA上相邻的5´-磷酸末端和3´-羟基末端形成磷酸二酯键。本试剂盒不仅适用于黏性末端连接，也兼容平滑末端和单碱基突出末端的DNA连接，优化的连接增强剂及反应缓冲液使反应更加高效便捷，25℃反应5 min即可快速连接，连接产物可直接转化多种化学感受态细胞。

5min Universal Ligation Mix

组分	C311-01（50 rxns）	C311-02（100 rxns）
2 × Universal Ligation Mix	250 μl	2 × 250 μl

5min Universal Ligation Mix

-30 ~ -15°C保存

lumiprobe -Universal CPG type II, 1000A

Products
DNA
CPG solid supports
Universal CPG type II, 1000A

Universal CPG type II, 1000A

Oligonucleotide synthesis CPG (controlled pore glass) solid supports for oligonucleotide synthesis

Cat. #	Quantity	Price	Lead time
2923-1g	1 g	45.00$
2923-10g	10 g	410.00$

Cat. #

Quantity

Price

Lead time

2923-1g

1 g

45.00$

2923-10g

10 g

410.00$

Bulk inquiry

The Universal CPG type II, 1000A is one of universal supports used to immobilize nucleosides for synthesizing oligonucleotides and to increase rate of dephosphorylation of the 3′ end oligonucleotide during deblocking.

For the cleavage from the support and oligonucleotide deprotection anhydrous ammonia gas-phase, ammonium hydroxide/methylamine mixture and other basic reagents can be used in a short time. The Universal CPG type II, 1000A is suitable for use in harsh conditions and makes cleavage and deprotection faster compared to universal supports. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides up to 120 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 2 hours at 80 °C or 8 hours at 55 °C using concentrated ammonia; 15 minutes at 65 °C using AMA mixture, ammonium hydroxide – 40% methylamine (1:1).

Oligo synthesis details

Pore size, Å:	1000
Typical loading, umol/g:	50−95
Coupling conditions:	standard coupling, identical to normal nucleobases
Cleavage conditions:	ammonium hydroxide 2 hours at 80 °C or 8 hours at 55 °C; AMA mixture, ammonium hydroxide – 40% methylamine (1:1), 15 minutes at 65 °C
Deprotection conditions:	identical to protected nucleobases

Your item has been added. View your cart or proceed to checkout

The count of items is incorrect. Universal CPG type II, 1000A

lumiprobe -Universal CPG type I, 500A

Products
DNA
CPG solid supports
Universal CPG type I, 500A

Universal CPG type I, 500A

Oligonucleotide synthesis CPG (controlled pore glass) solid supports for oligonucleotide synthesis

Cat. #	Quantity	Price	Lead time
2646-1g	1 g	39.00$
2646-10g	10 g	351.00$

Cat. #

Quantity

Price

Lead time

2646-1g

1 g

39.00$

2646-10g

10 g

351.00$

Bulk inquiry

The Universal CPG type I, 500A is one of universal supports used to immobilize nucleosides for high-throughput synthesizing oligonucleotides.

The cleavage from the support is followed by deprotection using anhydrous methylamine gas, ammonium hydroxide/methylamine mixture and other basic reagents. Universal support CPG type I is suitable for relatively aggressive environment treatment. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 8 hours at 80 °C using ammonium hydroxide or 17 hours at 55 °C; 3 hours at 80 °C using AMA mixture, ammonium hydroxide – 40% methylamine (1:1) or 8 hours at 55 °C using 40% methylamine or 30 minutes at 80 °C; 3 hours at 55 °C; 17 hours at room temperature using 0.4M NaOH in methanol – water (4:1).

Oligo synthesis details

Pore size, Å:	500
Typical loading, umol/g:	50−95
Coupling conditions:	standard coupling, identical to normal nucleobases
Cleavage conditions:	AMA mixture, ammonium hydroxide – 40% methylamine (1:1), 3 hours at 80 °C
Deprotection conditions:	identical to protected nucleobases

Your item has been added. View your cart or proceed to checkout

The count of items is incorrect. Universal CPG type I, 500A

标签： universal

Universal Plus mRNA-Seq 0520-A01

2. 12h-clock control of central dogma information flow by XBP1s

3．Acute Lengthening of Progenitor Mitosis Influences Progeny Fate during Cortical Development in vivo

Pages: 1-18 doi: 10.1159/000507113

4. ADAR1 mediated regulation of neural crest derived melanocytes and Schwann cell development

Pages: 198 doi: 10.1038/s41467-019-14090-5

5. Adenosinergic Signaling Alters Natural Killer Cell Functional Responses

Pages: 2533 doi: 10.3389/fimmu.2018.02533

6. Ancestral Folate Promotes Neuronal Regeneration in Serial Generations of Progeny

doi: 10.1007/s12035-019-01812-5

7. Best foot forward: Nanopore long reads, hybrid meta-assembly and haplotig purging optimises the first genome assembly for the Southern Hemisphere blacklip abalone (Haliotis rubra)

doi: 10.3389/fgene.2019.00889

8. Best Foot Forward: Nanopore Long Reads, Hybrid Meta-Assembly, and Haplotig Purging Optimizes the First Genome Assembly for the Southern Hemisphere Blacklip Abalone (Haliotis rubra)

doi: 10.3389/fgene.2019.00889

9. Brain-Mimetic Hydrogel Platform for Investigation of Glioblastoma Drug Resistance

10. Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora

doi: 10.1038/s41598-020-67097-0

金畔生物试剂,5min Universal Ligation Mix

lumiprobe -Universal CPG type II, 1000A

Universal CPG type II, 1000A

Usage

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lumiprobe -Universal CPG type I, 500A

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Usage

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