Sulfo-Cy5.5 dUTP

Sulfo-Cy5.5 dUTP,Sulfo-Cy5.5 是一种发射远红光的染料。 由于几乎没有荧光背景的生物源在其范围内发射,因此这种荧光团提供了很好的信噪比。,储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

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  • Sulfo-Cyanine5.5 dUTP

Sulfo-Cyanine5.5 dUTP

标签:PCR and qPCR, Triphosphates, sulfo-Cyanine5.5
货号 规格 价格 货期
2071-50nmol 50 nmol 290.00$ in stock
2071-250nmol 250 nmol 950.00$ 5 days

Sulfo-Cyanine5.5 is a far red emitting dye. Since almost no biological sources of fluorescence background emit in its range, this fluorophore provides great signal to noise ratio.

This molecule is a modified deoxyuridine triphosphate (dUTP) that is a substrate to many enzymes including simple Taq polymerase. Due to its extended amino-11-linker, this reagent provides a better alternative to aminoallyl derived triphosphates with dyes.

The QC of triphosphates includes HPLC-MS and enzymatic testing.

Sulfo-Cy5.5 是一种发射远红光的染料。 由于几乎没有荧光背景的生物源在其范围内发射,因此这种荧光团提供了很好的信噪比。

该分子是一种修饰的脱氧尿苷三磷酸 (dUTP),它是许多酶的底物,包括简单的 Taq 聚合酶。 由于其扩展的氨基 11 接头,该试剂为氨基烯丙基衍生的三磷酸盐和染料提供了更好的替代品。

三磷酸盐的 QC 包括 HPLC-MS 和酶促测试。

Absorption and emission spectra of sulfo-Cyanine5.5

Sulfo-Cyanine5.5 dUTP

Sulfo-Cy5.5 dUTP相关产品

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

胺反应性 Cy5 活化酯,用于标记蛋白质、肽和其他分子。

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5 azide 是一种用 Cy5(一种流行的荧光染料)进行 Click Chemistry 标记的试剂。

dsGreen for Real-Time PCR, 100×

Stock solution of dsGreen for Real-Time PCR, 100×

用于实时 PCR 的 dsGreen 储备溶液,100×

General properties

Appearance: blue solid / solution 蓝色固体/溶液
Molecular weight: 1554.97
Molecular formula: C58H63N6Li6O28P3S4
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 673
ε, L⋅mol−1⋅cm−1: 211000
Emission maximum, nm: 691
Fluorescence quantum yield: 0.21
CF260: 0.09
CF280: 0.11

Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP,Sulfo-Cy5 dUTP(脱氧尿苷三磷酸)是一种修饰的三磷酸核苷,用于 DNA 的酶标记。 三磷酸盐是 Cy5-dUTP 的类似物。 然而,与直接用染料修饰的氨基烯丙基 dUTP 不同,这种三磷酸盐含有更长的连接臂,可提高其被常用聚合酶(如 Taq 聚合酶)掺入的效率。

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  • Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP

标签:sulfo-Cyanine5, PCR and qPCR, Triphosphates
货号 规格 价格 货期
1510-50nmol 50 nmol 290.00$ in stock
1510-250nmol 250 nmol 950.00$ in stock

 

Sulfo-Cyanine5 dUTP (deoxyuridine triphosphate) is a modified nucleoside triphosphate for the enzymatic labeling of DNA. The triphosphate is an analog of Cy5-dUTP. However, unlike aminoallyl dUTP directly modified with dye, this triphosphate contains a longer linker arm that increases the efficiency of its incorporation by usual polymerases, like Taq polymerase.

Sulfo-Cyanine5 is a bright far red emitting dye for Cy5 channel.

The triphosphate is supplied in lyophilized form of lithium salt, ready to use.

Sulfo-Cy5 dUTP(脱氧尿苷三磷酸)是一种修饰的三磷酸核苷,用于 DNA 的酶标记。 三磷酸盐是 Cy5-dUTP 的类似物。 然而,与直接用染料修饰的氨基烯丙基 dUTP 不同,这种三磷酸盐含有更长的连接臂,可提高其被常用聚合酶(如 Taq 聚合酶)掺入的效率。

Sulfo-Cyanine5 是一种用于 Cy5 通道的明亮远红色发射染料。

三磷酸盐以锂盐的冻干形式提供,随时可用。

Absorption and emission spectra of sulfo-Cyanine5

Sulfo-Cyanine5 dUTP

Sulfo-Cy5 dUTP相关产品

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cyanine3 SE 活化酯。

Sulfo-Cyanine3 dUTP

Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3. The nucleotide contains a long linker that allows to incorporate the dye efficiently into DNA strand by various enzymes including Taq polymerase.

用于用磺基花青标记 DNA 的荧光 dNTP。 核苷酸包含一个长接头,允许通过包括 Taq 聚合酶在内的各种酶将染料有效地掺入 DNA 链中。

Sulfo-Cyanine5.5 dUTP

Modified deoxy triphosphate containing sulfo-Cyanine5.5 (aka Cy5.5) dye for enzymatic labeling of DNA microarrays, as well as PCR amplicons, and reverse transcripts.

含有磺基-Cyanine5.5(又名 Cy5.5)染料的改性脱氧三磷酸,用于 DNA 微阵列以及 PCR 扩增子和逆转录物的酶标记。

General properties

Appearance: blue solid / solution 蓝色固体/溶液
Molecular weight: 1275.89
Molecular formula: C49H66N6Li4O22P3S2
Solubility: good in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Sulfo-Cy3 dUTP

Sulfo-Cy3 dUTP,标记的脱氧核苷三磷酸 (dNTPs) 为 DNA 的酶标记提供了一种有效的方法。 DNA 微阵列、PCR 扩增子和逆转录物可以使用各种 dNTP 进行标记。

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Sulfo-Cyanine3 dUTP

标签:PCR and qPCR,Triphosphates,sulfo-Cy3
货号 规格 价格 货期
1511-50nmol 50 nmol 290.00$ in stock
1511-250nmol 250 nmol 950.00$ in stock

Labeled deoxynucleoside triphosphates (dNTPs) provide an efficient way for the enzymatic labeling of DNA. DNA microarrays, PCR amplicons, and reverse transcripts can be labeled using various dNTPs.

Sulfo-Cyanine3 is a dye emitting in yellow range. This fluorophore is a standard dye used in DNA microarray experiments. Sulfo-Cyanine3 dUTP is a labeled triphosphate providing efficient labeling in enzymatic reactions with various enzymes, including simple Taq polymerase. Quality of each batch is assured by HPLC-MS, and testing in enzymatic reaction.

标记的脱氧核苷三磷酸 (dNTPs) 为 DNA 的酶标记提供了一种有效的方法。 DNA 微阵列、PCR 扩增子和逆转录物可以使用各种 dNTP 进行标记。

Sulfo-Cyanine3 是一种在黄色范围内发光的染料。 这种荧光团是 DNA 微阵列实验中使用的标准染料。 Sulfo-Cyanine3 dUTP 是一种标记的三磷酸盐,可在与各种酶(包括简单的 Taq 聚合酶)的酶促反应中提供有效标记。 每批产品的质量均通过 HPLC-MS 和酶促反应检测来保证。

Absorption and emission spectra of sulfo-Cyanine3

Sulfo-Cyanine3 dUTP

Sulfo-Cy3 dUTP相关产品

Cyanine3.5 NHS ester

Cyanine3.5 dye NHS ester derivative.

Cy3.5染料NHS酯衍生物。

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.

胺反应性 Cy3 染料 NHS 酯。

Sulfo-Cyanine5.5 dUTP

Modified deoxy triphosphate containing sulfo-Cyanine5.5 (aka Cy5.5) dye for enzymatic labeling of DNA microarrays, as well as PCR amplicons, and reverse transcripts.

含有磺基-Cyanine5.5(又名 Cy5.5)染料的改性脱氧三磷酸,用于 DNA 微阵列以及 PCR 扩增子和逆转录物的酶标记。

General properties

Appearance: red solid / solution红色固体/溶液
Molecular weight: 1247.84
Molecular formula: C47H62N6Li4O22P3S2
Solubility: good in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

FAM-11-dUTP, 6-isomer

FAM-11-dUTP, 6-isomer,6-FAM-11-2′-deoxyuridine-5′-triphosphate,三锂盐,是非放射性 DNA 标记的常用试剂​​。

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  • FAM-11-dUTP, 6-isomer

FAM-11-dUTP, 6-isomer

标签:FAM (Fluorescein), PCR and qPCR, Triphosphates
货号 规格 价格 货期
2077-50nmol 50 nmol 190.00$ in stock
2077-250nmol 250 nmol 750.00$ in stock
2077-500nmol 500 nmol 1200.00$ 7 days
2077-1umol 1 umol please inquire in stock

6-FAM-11-2’-deoxyuridine-5’-triphosphate, trilithium salt, is a common agent for non-radioactive DNA labeling.

FAM (fluorescein) is a popular green-fluorescent dye with an emission maximum at 513 nm. This derivative is a pure FAM 6-isomer.

6-FAM-11-dUTP is a modified triphosphate that can be incorporated into DNA instead of dTTP by various enzymes, including Klenow fragment, Taq-Polymerase, DNA-Polymerase I, Phi 29 Polymerase, reverse transcriptase or terminal transferase.

In contrast to triphosphates with a dye directly linked to aminoallyl-dUTP, this triphosphate contains a linker of 11 atoms between the fluorophore and the nitrogenous base. This linker length prevents FAM-label from potential static quenching and increases the efficiency of nucleotide incorporation during DNA synthesis.

6-FAM-11-dUTP can be used to produce a labeled product during PCR and cDNA synthesis and for labeled DNA-probes synthesis by random primed labeling and Nick-translation. Synthesized fluorescence-labeled DNA probes can be used to detect specific sequences by Southern blot, Northern blot, in situ hybridization, or by microarray analysis.

6-FAM-11-2′-deoxyuridine-5′-triphosphate,三锂盐,是非放射性 DNA 标记的常用试剂​​。

FAM(荧光素)是一种流行的绿色荧光染料,最大发射波长为 513 nm。该衍生物是纯 FAM 6 异构体。

6-FAM-11-dUTP 是一种修饰的三磷酸盐,可以通过各种酶(包括 Klenow 片段、Taq-聚合酶、DNA-聚合酶 I、Phi 29 聚合酶、逆转录酶或末端转移酶)掺入 DNA 中,而不是 dTTP。

与染料直接连接到氨基烯丙基-dUTP 的三磷酸盐相比,这种三磷酸盐在荧光团和含氮碱基之间含有 11 个原子的接头。这种接头长度可防止 FAM 标记潜在的静态猝灭,并提高 DNA 合成过程中核苷酸掺入的效率。

6-FAM-11-dUTP 可用于在 PCR 和 cDNA 合成过程中产生标记产物,以及通过随机引物标记和尼克翻译合成标记的 DNA 探针。合成的荧光标记 DNA 探针可用于通过Southern印迹、Northern印迹、原位杂交或微阵列分析检测特定序列。

Absorption and emission spectra of FAM

FAM-11-dUTP, 6-isomer

FAM-11-dUTP, 6-isomer相关产品

Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP is a fluorescent labeled triphosphate for the enzymatic modification of DNA with sulfo-Cyanine5 fluorophore. The reagent contains a long linker arm, its incorporation is highly efficient.

Sulfo-Cy5 dUTP 是一种荧光标记的三磷酸盐,用于用 sulfo-Cy5 荧光团对 DNA 进行酶促修饰。 该试剂含有一个长连接臂,它的掺入效率很高。

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.

Cy5 NHS 酯最小染料用于蛋白质标记。

Amino-11-dUTP

Amino-11-dUTP is a triphosphate for the modification of DNA.

Amino-11-dUTP 是一种用于修饰 DNA 的三磷酸盐。

General properties

Appearance: yellow-greenish solid 黄绿色固体
Molecular weight: 1013.49
Molecular formula: C39H39N4Li3O21P3
IUPAC name: ((2R,3S,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17

FAM-11-dCTP, 6-isomer

FAM-11-dCTP, 6-isomer,脱氧胞苷三磷酸 (dCTP) 的 6-荧光素 (FAM) 衍生物。FAM 6 异构体是用于标记生物分子的常见荧光团; FAM 具有高量子产率和亮绿色发射。

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  • FAM-11-dCTP, 6-isomer

FAM-11-dCTP, 6-isomer

标签:FAM (Fluorescein), PCR and qPCR, Triphosphates
货号 规格 价格 货期
2713-50nmol 50 nmol 190.00$ in stock
2713-250nmol 250 nmol 750.00$ in stock
2713-500nmol 500 nmol 1200.00$ in stock
2713-1umol 1 umol please inquire in stock
6-fluorescein (FAM) derivative of deoxycytidine triphosphate (dCTP).

FAM 6-isomer is a common fluorophore used for labeling biomolecules; FAM has a high quantum yield and bright green emission.

FAM-11-dCTP can be used for enzymatic DNA labeling via Nick-translation, 3’-end labeling, random primed labeling, PCR, or cDNA synthesis. FAM-11-dCTP is incorporated into the growing DNA chain by Taq-polymerase, DNA-polymerase I, Phi 29 polymerase, Klenow fragment, reverse transcriptase, or terminal transferase.

The linker length (11 carbon atoms) is optimal for the efficient incorporation of fluorescently-labeled dCTP into the growing DNA chain.

Labeled DNA can be used for FISH experiments, microarray gene profiling, Southern blot, and Northern blot.

脱氧胞苷三磷酸 (dCTP) 的 6-荧光素 (FAM) 衍生物。
FAM 6 异构体是用于标记生物分子的常见荧光团; FAM 具有高量子产率和亮绿色发射。

FAM-11-dCTP 可用于通过尼克翻译、3′ 端标记、随机引物标记、PCR 或 cDNA 合成进行酶促 DNA 标记。 FAM-11-dCTP 通过 Taq 聚合酶、DNA 聚合酶 I、Phi 29 聚合酶、Klenow 片段、逆转录酶或末端转移酶结合到不断增长的 DNA 链中。

接头长度(11 个碳原子)对于将荧光标记的 dCTP 有效掺入正在生长的 DNA 链中是最佳的。

标记的 DNA 可用于 FISH 实验、微阵列基因分析、Southern 印迹和 Northern 印迹。

Absorption and emission spectra of FAM

FAM-11-dCTP, 6-isomer

FAM-11-dCTP, 6-isomer相关产品

AF568 NHS ester

AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.

AF568 衍生物,用于用游离胺标记蛋白质和其他高分子量和低分子量配体。

FAM-11-dCTP, 6-isomer Add this product to your cart and
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dsGreen for Real-Time PCR, 100×

Stock solution of dsGreen for Real-Time PCR, 100×

用于实时 PCR 的 dsGreen 储备溶液,100×

FAM-11-dCTP, 6-isomer Add this product to your cart and
get free express delivery

General properties

Appearance: yellow/orange solid 黄色/橙色固体
Molecular weight: 1012.5
Molecular formula: C39H40N5Li3O20P3
IUPAC name: ((2R,3S,5R)-5-(4-amino-5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2-oxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: good in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17

FAM-11-dCTP, 6-isomer

FAM-11-UTP, 6-isomer

FAM-11-UTP, 6-isomer,尿苷三磷酸 (UTP) 的 6-荧光素 (FAM) 衍生物。 FAM 是一种具有高量子产率的荧光团。 在绿色光谱范围内,荧光的最大发射波长为 513 nm。

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  • FAM-11-UTP, 6-isomer

FAM-11-UTP, 6-isomer

标签:FAM (Fluorescein), PCR and qPCR, Triphosphates
货号 规格 价格 货期
2714-50nmol 50 nmol 190.00$ 21 days
2714-250nmol 250 nmol 750.00$ 21 days
2714-500nmol 500 nmol 1200.00$ 21 days
2714-1umol 1 umol please inquire 21 days

6-fluorescein (FAM) derivative of uridine triphosphate (UTP). FAM is a fluorophore with a high quantum yield. The maximum emission of the fluorescence is at a wavelength of 513 nm in the green spectrum range.

6-FAM-11-UTP can be used as a substrate for RNA-polymerases T7, T3 and SP6 during in vitro transcription. RNA-probes produced with this method can be used for fluorescence hybridization in situ, including multiplex, and for Northern blot. Fluorescently-labeled cRNA can be used for microarray-based gene expression profiling.

尿苷三磷酸 (UTP) 的 6-荧光素 (FAM) 衍生物。 FAM 是一种具有高量子产率的荧光团。 在绿色光谱范围内,荧光的最大发射波长为 513 nm。

6-FAM-11-UTP 可在体外转录过程中用作 RNA 聚合酶 T7、T3 和 SP6 的底物。 用这种方法产生的 RNA 探针可用于原位荧光杂交,包括多重荧光杂交和 Northern 印迹。 荧光标记的 cRNA 可用于基于微阵列的基因表达谱分析。

Absorption and emission spectra of FAM

FAM-11-UTP, 6-isomer

FAM-11-UTP, 6-isomer相关产品

Amino-11-UTP

Uridine triphosphate labeled with amino group intended for indirect non-radioactive enzymatic RNA labeling.

用氨基标记的三磷酸尿苷,用于间接非放射性酶促 RNA 标记。

FAM-11-dUTP, 6-isomer

FAM-derivative of dUTP with green emission for DNA labeling.

用于 DNA 标记的具有绿色发射的 dUTP 的 FAM 衍生物。

Biotin-11-UTP

Biotinylated UTP for enzymatic RNA labeling.

用于酶促 RNA 标记的生物素化 UTP。

General properties

Appearance: yellow/orange solid 黄色/橙色固体
Molecular weight: 1029.48
Molecular formula: C39H39N4Li3O22P3
IUPAC name: ((2R,3S,4R,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17

Biotin-11-dUTP

Biotin-11-dUTP,生物素化脱氧尿苷三磷酸 (dUTP) 可用于使用各种方法进行 DNA 标记,包括尼克翻译、聚合酶链式反应 (PCR)、随机引发和 3′-末端非放射性标记。 生成的探针可用于各种杂交实验,例如Southern印迹、Northern印迹、斑点印迹或FISH。 用辣根过氧化物酶偶联的链霉亲和素或其他结合生物素的生物分子偶联物检测生物素标记的探针。

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  • Biotin-11-dUTP

Biotin-11-dUTP

标签:PCR and qPCR, Triphosphates
货号 规格 价格 货期
1553-250nmol 250 nmol in stock
1553-500nmol 500 nmol 190.00$ in stock
1553-1umol 1 umol 290.00$ in stock
1553-5umol 5 umol please inquire 21 days

Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation, polymerase chain reaction (PCR), random priming, and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.

«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.

生物素化脱氧尿苷三磷酸 (dUTP) 可用于使用各种方法进行 DNA 标记,包括尼克翻译、聚合酶链式反应 (PCR)、随机引发和 3′-末端非放射性标记。 生成的探针可用于各种杂交实验,例如Southern印迹、Northern印迹、斑点印迹或FISH。 用辣根过氧化物酶偶联的链霉亲和素或其他结合生物素的生物分子偶联物检测生物素标记的探针。

化合物名称中的 «11» 表示 dUTP 和生物素之间的接头长度; 该接头提高了标记的 dUTP 掺入 DNA 的效率以及生物素与特定蛋白质(如抗生物素蛋白/链霉抗生物素蛋白或抗生物素抗体)的相互作用。

Biotin-11-dUTP相关产品

Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP is a fluorescent labeled triphosphate for the enzymatic modification of DNA with sulfo-Cyanine5 fluorophore. The reagent contains a long linker arm, its incorporation is highly efficient.

Sulfo-Cy5 dUTP 是一种荧光标记的三磷酸盐,用于用 sulfo-Cy5 荧光团对 DNA 进行酶促修饰。 该试剂含有一个长连接臂,它的掺入效率很高。

Amino-11-dUTP

Amino-11-dUTP is a triphosphate for the modification of DNA.

Amino-11-dUTP 是一种用于修饰 DNA 的三磷酸盐。

FAM-11-dUTP, 6-isomer

FAM-derivative of dUTP with green emission for DNA labeling.

用于 DNA 标记的具有绿色发射的 dUTP 的 FAM 衍生物。

General properties

Appearance: colorless solid 无色固体
Molecular weight: 881.48
Molecular formula: C28H43N6Li3O17P3S
IUPAC name: ((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免过多的冻融循环。

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Biotin-11-dCTP

Biotin-11-dCTP,Triphosphates,DNA,Modified triphosphates,Biotin-11-dCTP 是一种脱氧胞苷三磷酸 (dCTP),通过 11 原子接头与生物素分子连接,可与亲和素和链霉亲和素有效相互作用; 与天然 dCTP 的掺入过程一样。

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Biotin-11-dCTP

标签:Triphosphates,DNA,Modified triphosphates
货号 规格 价格 货期
2715-250nmol 250 nmol 21 days
2715-500nmol 500 nmol 190.00$ 21 days
2715-1umol 1 umol 290.00$ 21 days
2515-5umol 5 umol please inquire 21 days

Biotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; incorporation into DNA proceeds as with natural dCTP.

Biotin-11-dCTP is a substrate for Klenow fragment, T4 and Taq DNA-polymerases, reverse transcriptase, and terminal transferase. Biotinylated DNA can be detected using alkaline phosphatase-conjugated avidin/streptavidin or relevant fluorescent-labeled antibodies.

Biotin-labeled DNA/cDNA probes can be used for fluorescent hybridization in situ or microarray gene profiling.

Biotin-11-dCTP 是一种脱氧胞苷三磷酸 (dCTP),通过 11 原子接头与生物素分子连接,可与亲和素和链霉亲和素有效相互作用; 与天然 dCTP 的掺入过程一样。

Biotin-11-dCTP 是 Klenow 片段、T4 和 Taq DNA 聚合酶、逆转录酶和末端转移酶的底物。 可以使用碱性磷酸酶偶联的亲和素/链霉亲和素或相关的荧光标记抗体检测生物素化的 DNA。

生物素标记的 DNA/cDNA 探针可用于荧光原位杂交或微阵列基因分析。

Biotin-11-dCTP相关产品

FAM-11-dCTP, 6-isomer

6-FAM-labeled dCTP for enzymatic DNA labeling.

用于酶促 DNA 标记的 6-FAM 标记的 dCTP。

Biotin-11-dUTP

Biotin-labeled deoxyuridine triphosphate for enzymatic incorporation into DNA and indirect detection.

生物素标记的脱氧尿苷三磷酸,用于酶促掺入 DNA 和间接检测。

General properties

Appearance: colorless solid 无色固体
Molecular weight: 883.52
Molecular formula: C28H47N7Li3O16P3S
IUPAC name: ((2R,3S,5R)-5-(4-amino-2-oxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免过多的冻融循环。

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Biotin-11-UTP

Biotin-11-UTP,Biotin-11-UTP 是 RNA 聚合酶 SP6、T3 和 T7 的底物。 生物素化三磷酸是天然 UTP 的类似物,可用于体外转录中的 RNA 标记。

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Biotin-11-UTP

标签:Triphosphates,Modified triphosphates,DNA
货号 规格 价格 货期
2716-250nmol 250 nmol 7 days
2716-500nmol 500 nmol 190.00$ in stock
2716-1umol 1 umol 290.00$ in stock
2716-5umol 5 umol please inquire in stock

Biotin-11-UTP is a substrate for RNA-polymerases SP6, T3, and T7. Biotinylated triphosphate is an analog of natural UTP and can be used for RNA labeling in transcription in vitro.

Biotinylated RNA can be used instead of radioactive-labeled RNA in many applications, including Northern and Southern blots, hybridization in situ, and microarray analysis. Biotinylated RNA is detected using various methods with streptavidin conjugates, and the long linker of 11 atoms allows efficient interaction of biotin with streptavidin.

Biotin-11-UTP 是 RNA 聚合酶 SP6、T3 和 T7 的底物。 生物素化三磷酸是天然 UTP 的类似物,可用于体外转录中的 RNA 标记。

生物素标记的 RNA 可以在许多应用中代替放射性标记的 RNA,包括 Northern 和 Southern 印迹、原位杂交和微阵列分析。 生物素化的 RNA 使用各种方法与链霉亲和素偶联物进行检测,11 个原子的长接头允许生物素与链霉亲和素的有效相互作用。

Biotin-11-UTP相关产品

Biotin-11-dUTP

Biotin-labeled deoxyuridine triphosphate for enzymatic incorporation into DNA and indirect detection.

生物素标记的脱氧尿苷三磷酸,用于酶促掺入 DNA 和间接检测。

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Amino-11-UTP

Uridine triphosphate labeled with amino group intended for indirect non-radioactive enzymatic RNA labeling.

用氨基标记的三磷酸尿苷,用于间接非放射性酶促 RNA 标记。

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General properties

Appearance: colorless solid
Molecular weight: 894.48
Molecular formula: C28H43N6Li3O18P3S
IUPAC name: ((2R,3S,4R,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: good in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免过多的冻融循环。

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Biotin-11-UTP

Amino-11-ddUTP

Amino-11-ddUTP,Amino-11-ddUTP 是一种终止子三磷酸,用于 DNA 的酶末端标记。 核苷酸通过 Taq DNA 聚合酶、Sequenase、Klenow 片段掺入。 氨基末端 DNA 与活化酯的后续反应可用于 DNA 的末端标记。

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  • Amino-11-ddUTP

Amino-11-ddUTP

标签:PCR and qPCR, Triphosphates,DNA
货号 规格 价格 货期
A5040 200 nmol 190.00$ 10 days
15040 1 umol 549.00$ in stock
25040 5 umol 1590.00$ in stock
45040 25 umol 3975.00$ in stock

Amino-11-ddUTP is a terminator triphosphate for enzymatic end-labeling of DNA. The nucleotide is incorporated by Taq DNA polymerase, Sequenase, Klenow fragment. Subsequent reaction of amino-terminated DNA with activated esters can be used for the end-labeling of DNA.

Amino-11-ddUTP 是一种终止子三磷酸,用于 DNA 的酶末端标记。 核苷酸通过 Taq DNA 聚合酶、Sequenase、Klenow 片段掺入。 氨基末端 DNA 与活化酯的后续反应可用于 DNA 的末端标记。

Amino-11-ddUTP相关产品

Cyanine7 azide

Near-infrared fluorescent dye for Click Chemistry.

用于点击化学的近红外荧光染料。

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cy3 SE 活化酯。

Cyanine7 NHS ester

Amine reactive NHS ester of near infrared dye Cyanine7.

近红外染料Cy7的胺活性NHS酯。

General properties

Appearance: colorless solid
Molecular weight: 960.44
CAS number: 439077-17-5 (without cation and water of crystallization)
Molecular formula: C18H62Li3N4O32P3
Solubility: good in water
Quality control: NMR 1H and 31P, HPLC-MS (99+%)
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存:-20°C 收货后 24 个月。 运输:在室温下长达 3 周。 干燥。

MSDS: Download
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相关文献:

  1. Bakker, R.; Mani, M.; Carthew, R.W. The Wg and Dpp morphogens regulate gene expression by modulating the frequency of transcriptional bursts. bioRxiv, preprint. doi: 10.1101/2020.01.24.918623
  2. Boersma, S.; Rabouw, H.H.; Bruurs, L.J.M.; Pavlovič, T.; van Vliet, A.L.W.; Beumer, J.; Clevers, H.; van Kuppeveld, F.J.M.; Tanenbaum, M.E. Translation and Replication Dynamics of Single RNA Viruses. Cell, 2020, 183(7), 1930–1945.e23. doi: 10.1016/j.cell.2020.10.019
  3. Yu, Y.; Guo, Y.; Tian, Q.; Lan, Y.; Yeh, H.; Zhang, M.; Tasan, I.; Jain, S.; Zhao, H. An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms. Nature Chemical Biology, 2020, 16(4), 387–390. doi: 10.1038/s41589-019-0432-1
  4. Hampoelz, B.; Schwarz, A.; Ronchi, P.; Bragulat-Teixidor, H.; Tischer, C.; Gaspar, I.; Ephrussi, A.; Schwab, Y.; Beck, M. Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis. Cell, 2019, 179, 671–686.e17. doi: 10.1016/j.cell.2019.09.022

Amino-11-dUTP

Amino-11-dUTP,Amino-11-dUTP 是氨基烯丙基 dUTP 的改进替代品。 这种三磷酸盐具有更长的接头,可以更轻松地进行下游标记。

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  • Modified triphosphates
  • Amino-11-dUTP

Amino-11-dUTP

标签:PCR and qPCR, Triphosphates
货号 规格 价格 货期
16040 1 umol 95.00$ in stock
26040 5 umol 195.00$ in stock
46040 25 umol 395.00$ in stock

Amino-11-dUTP is an improved replacement for Aminoallyl dUTP. This triphosphate features longer linker, which allows for easier and more facile downstream labeling.

Amino-11-dUTP is compatible with PCR, nick translation, and reverse transcription. It can be used for microarray labeling. The nucleotide is incorporated by Taq DNA polymerase, Klenow fragment, and reverse transcriptases. Subsequent reaction of amino-DNA with activated esters can be used for the labeling of DNA.

Amino-11-dUTP 是氨基烯丙基 dUTP 的改进替代品。 这种三磷酸盐具有更长的接头,可以更轻松地进行下游标记。

Amino-11-dUTP 与 PCR、缺口平移和逆转录兼容。 它可用于微阵列标记。 核苷酸通过 Taq DNA 聚合酶、Klenow 片段和逆转录酶掺入。 氨基-DNA 与活化酯的后续反应可用于标记 DNA。

Amino-11-dUTP相关产品

TAMRA azide, 5-isomer

TAMRA fluorescent dye azide for Click chemistry.

用于点击化学的 TAMRA 荧光染料叠氮化物。

Cyanine7 amine

Cy7 amine is a NIR dye with free amine group.

Cy7 胺是一种带有游离胺基的 NIR 染料。

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Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.

Cy5.5 NHS 是远红/近红外胺活性染料。

General properties

Appearance: colorless solid 无色固体
Molecular weight: 976.44
Molecular formula: C18H62Li3N4O33P3
Solubility: good in water
Quality control: NMR 1H and 31P, HPLC (99+%), PCR testing
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存:-20°C 收货后 24 个月。 运输:在室温下长达 3 周。 干燥。

MSDS: Download
Product specifications

Amino-11-UTP

Amino-11-UTP,氨基-UTP 是带有氨基的三磷酸尿苷,设计用于在使用 T7、T3 和 SP6 RNA 聚合酶进行体外转录期间将氨基酶促掺入 RNA 分子中。 酶促反应产生带有氨基的 RNA 分子,可与胺反应性试剂(如荧光染料的 NHS 酯或生物素)结合。

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  • Amino-11-UTP

Amino-11-UTP

标签:Triphosphates,DNA
货号 规格 价格 货期
2460-1umol 1 umol 95.00$ 7 days
2460-5umol 5 umol 195.00$ 10 days
2460-25umol 25 umol 395.00$ in stock

Amino-UTP is uridine triphosphate with an amino group, which is designed for enzymatic incorporation of the amino group into the RNA molecule during in vitro transcription using T7, T3, and SP6 RNA polymerases. The enzymatic reaction results in the RNA molecule with amino groups, which can be conjugated with amine-reactive reagents such as NHS esters of fluorescent dyes or biotin.

The amino group is separated from uridine with a long linker of 11 atoms; it is longer than frequently used C3-allyl linker and prevents the fluorophore from potential static quenching after RNA labeling via the amino group. The efficacy of RNA synthesis using amino-11-UTP is almost the same as that when using non-labeled nucleotide triphosphates.

Incorporation of amino-11-UTP with subsequent labeling via the amino group is used for cRNA synthesis and microarray analysis.

氨基-UTP 是带有氨基的三磷酸尿苷,设计用于在使用 T7、T3 和 SP6 RNA 聚合酶进行体外转录期间将氨基酶促掺入 RNA 分子中。 酶促反应产生带有氨基的 RNA 分子,可与胺反应性试剂(如荧光染料的 NHS 酯或生物素)结合。

氨基通过 11 个原子的长接头与尿苷分离; 它比常用的 C3-烯丙基接头更长,可防止荧光团在通过氨基标记 RNA 后发生潜在的静态猝灭。 使用氨基 11-UTP 合成 RNA 的效果几乎与使用未标记的三磷酸核苷酸相同。

将氨基-11-UTP 与随后通过氨基标记的结合用于 cRNA 合成和微阵列分析。

Amino-11-UTP相关产品

Sulfo-Cyanine3 dUTP

Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3. The nucleotide contains a long linker that allows to incorporate the dye efficiently into DNA strand by various enzymes including Taq polymerase.

用于用磺基花青标记 DNA 的荧光 dNTP。 核苷酸包含一个长接头,允许通过包括 Taq 聚合酶在内的各种酶将染料有效地掺入 DNA 链中。

Amino-11-dUTP

Amino-11-dUTP is a triphosphate for the modification of DNA.

Amino-11-dUTP 是一种用于修饰 DNA 的三磷酸盐。

Amino-11-UTP Add this product to your cart and
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Amino-11-ddUTP

Amino-11-ddUTP is a triphosphate for the modification of DNA.

Amino-11-ddUTP 是一种用于修饰 DNA 的三磷酸盐。

Amino-11-UTP Add this product to your cart and
get free express delivery

General properties

Appearance: off white solid
Molecular weight: 920.37
Molecular formula: C18H54N4Li3O30P3
Solubility: good in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存:-20°C 收货后 24 个月。 运输:在室温下长达 3 周。 干燥。

MSDS: Download
Product specifications