标签： Trefoil Factor-1 Human Recombinant

TFF-1, TFF1, pS2, BCEI, HPS, HP1.A, pNR-2, D21S21, pS2 protein, Trefoil factor 1, Breast cancer estrogen-inducible protein.

The Trefoil Factor peptides are stable secretory proteins expressed in the gastrointestinal tract , and are involved in intestinal mucosal defense and repair. TFF1 is an essential protein for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene. TFF1 is a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. TFF1 protects the mucosa from isults, stabilizes the mucus layer, & affects healing of the epithelium. TFF1 is commonly expressed in tumors. TFF1 is related with the cell membrane of MCF-7 cells. High levels of TFF1 and TFF2 are found in serum from inflammatory bowel disease.

三叶因子肽是在胃肠道中表达的稳定分泌蛋白，参与肠黏膜的防御和修复。 TFF1 是胃窦和幽门胃粘膜正常分化的必需蛋白，并作为胃特异性肿瘤抑制基因发挥作用。 TFF1 是覆盖在胃肠道黏膜上的黏液凝胶的稳定剂，可提供抵抗各种有害物质的物理屏障。 TFF1 保护粘膜免受损伤，稳定粘液层，并影响上皮的愈合。 TFF1 通常在肿瘤中表达。 TFF1与MCF-7细胞的细胞膜有关。 在炎症性肠病的血清中发现了高水平的 TFF1 和 TFF2。

TFF-1 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 60 amino acids which includes a 40 amino acid trefoil motif containing 3 conserved intramolecular disulfide bonds and having a total molecular mass of 13.2 kDa.
TFF-1 Human Recombinant is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TFF-1 Human Recombinant 是一种同源二聚体、非糖基化多肽链，含有 2 x 60 个氨基酸，其中包括一个 40 个氨基酸的三叶基序，该基序含有 3 个保守的分子内二硫键，总分子量为 13.2 kDa。
TFF-1 Human Recombinant 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The Human TFF1 protein was lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4 and 150mM NaCl.

It is recommended to reconstitute the lyophilized TFF1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized TFF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TFF1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TFF1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

EAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFY
PNTIDVPPEEECEF.

The ED₅₀ as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10µg/ml, corresponding to a specific activity of >100 IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.