标签： sulfo-Cyanine7

Sulfo-Cyanine7 tetrazine

Sulfo-Cyanine7 tetrazine,Sulfo-Cy7 是一种近红外水溶性荧光团。 该衍生物含有甲基四嗪基团，可在称为 TCO 连接的反应中与环烯烃进行快速、高效且无金属的共轭。

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  • Sulfo-Cyanine7 tetrazine

Sulfo-Cyanine7 tetrazine

标签：sulfo-Cyanine7,sulfo-Cy7, Near infrared (NIR) imaging, Tetrazine, Copper free Click chemistry
货号 规格 价格 货期
153E0 1 mg 110.00$ in stock
253E0 5 mg 290.00$ in stock
453E0 25 mg 690.00$ in stock
553E0 50 mg 1270.00$ in stock
653E0 100 mg 1990.00$ in stock

Sulfo-Cyanine7 is a near infrared, water soluble fluorophore. This derivative contains methyltetrazine group for the fast, efficient, and metal free conjugation with cycloalkenes in a reaction called TCO ligation.

Sulfo-Cy7 是一种近红外水溶性荧光团。 该衍生物含有甲基四嗪基团，可在称为 TCO 连接的反应中与环烯烃进行快速、高效且无金属的共轭。

Sulfo-Cyanine7 absorption and emission spectra

Sulfo-Cyanine7 tetrazine

Sulfo-Cyanine7 tetrazine相关产品

Cyanine3 tetrazine

Tetrazine derivative of the fluorophore Cyanine3. Tetrazines react extremely quickly with strained alkenes (such as trans-cyclooctenes, cyclopropenes), and some strained alkynes.

荧光团 Cy3 的四嗪衍生物。 四嗪与应变烯烃（如反式环辛烯、环丙烯）和一些应变炔烃反应极快。

AF430 tetrazine

Tetrazine derivative of AF430 fluorescent dye suitable for in vitro cell labeling.

AF430 荧光染料的四嗪衍生物，适用于体外细胞标记。

Sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.

NHS 酯形式的水溶性磺化远红色磺基-Cyanine5.5 染料。

General properties

Appearance: dark green solid  深绿色固体
Mass spec M+ increment: 863.3
Molecular weight: 930.19
Molecular formula: C47H52N7KO7S2
Solubility: good in DMF, DMSO, water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04

Sulfo-Cyanine7 amine

Sulfo-Cyanine7 amine,Cyanine7 NIR 染料的磺化、水溶性氨基衍生物。这种 NIR 染料胺可用于通过与亲电子基团的反应以及涉及氨基转移的酶促反应衍生各种靶标。

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  • Sulfo-Cyanine7 amine

Sulfo-Cyanine7 amine

标签：sulfo-Cyanine7, Amines, Near infrared (NIR) imaging
货号 规格 价格 货期
153C0 1 mg 110.00$ in stock
253C0 5 mg 290.00$ in stock
453C0 25 mg 690.00$ in stock
553C0 50 mg 1270.00$ in stock
653C0 100 mg 1990.00$ in stock

Sulfonated, water-soluble, amino derivative of Cyanine7 NIR dye.

This NIR dye amine can be used for the derivatization of various targets by the reaction with electrophilic groups, and also by enzymatical reactions involving transamination.

Cyanine7 NIR 染料的磺化、水溶性氨基衍生物。

这种 NIR 染料胺可用于通过与亲电子基团的反应以及涉及氨基转移的酶促反应衍生各种靶标。

Sulfo-Cyanine7 absorbance and emission spectra

Sulfo-Cyanine7 amine

Sulfo-Cyanine7 amine相关产品

Cyanine5 carboxylic acid

Cyanine5 carboxylic acid, unactivated dye.

Cy5羧酸，未活化染料。

ROX DBCO, 5-isomer

ROX (rhodamine X) is a bright rhodamine dye. This is a DBCO (dibenzocyclooctyne) derivative that has high reactivity against azides.

ROX（罗丹明 X）是一种明亮的罗丹明染料。 这是一种对叠氮化物具有高反应性的 DBCO（二苯并环辛炔）衍生物。

Amino-11-dUTP

Amino-11-dUTP is a triphosphate for the modification of DNA.

Amino-11-dUTP 是一种用于修饰 DNA 的三磷酸盐。

General properties

Appearance: dark green powder 深绿色粉末
Molecular weight: 807.07
CAS number: 2236573-39-8
Molecular formula: C43H58N4O7S2
Solubility: very poorly soluble in water (0.50 mM = 40 mg/L), good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04

相关文献：

  1. Kim, H.; Han, J.; Park, J.-H. Cyclodextrin polymer improves atherosclerosis therapy and reduces ototoxicity. Journal of Controlled Release, 2020, 319, 77–86. doi: 10.1016/j.jconrel.2019.12.021
  2. Rippe, M.; Stefanello, T.F.; Kaplum, V.; Britta, E.A.; Garcia, F.P.; Poirot, R.; Companhoni, M.V.P.; Nakamura, C.V.; Szarpak-Jankowska, A.; Auzély-Velty, R. Heparosan as a potential alternative to hyaluronic acid for the design of biopolymer-based nanovectors for anticancer therapy. Biomaterials Science, 2019, 7, 2850–2860. doi: 10.1039/c9bm00443b
  3. Guo, X.; Zou, C.-L.; Jung, H.; Gong, Z.; Bruch, A.; Jiang, L.; Tang, H.X. Efficient Generation of a Near-visible Frequency Comb via Cherenkov-like Radiation from a Kerr Microcomb. Physical Review Applied, 2018, 10(1), 014012. doi: 10.1103/PhysRevApplied.10.014012
  4. Tabe, H.; Sukenobe, K.; Kondo, T.; Sakurai, A.; Maruo, M.; Shimauchi, A.; Hirano, M.; Uno, S.-N.; Kamiya, M.; Urano, Y.; Matsushita, M.; Fujiyoshi, S. Cryogenic Fluorescence Localization Microscopy of Spectrally Selected Individual FRET Pairs in a Water Matrix. The Journal of Physical Chemistry B, 2018, 122(27), 6906–6911. doi: 10.1021/acs.jpcb.8b03977
Cy® is a trademark of GE Healthcare.

sulfo-Cyanine7 dicarboxylic acid

sulfo-Cyanine7 dicarboxylic acid,Sulfo-Cyanine7 二羧酸（Сy7 类似物）是一种水溶性双功能 NIR 染料衍生物，带有两个羧酸官能团。

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  • sulfo-Cyanine7 dicarboxylic acid

sulfo-Cyanine7 dicarboxylic acid

标签：sulfo-Cyanine7,sulfo-Cy7, Carboxylic acids
货号 规格 价格 货期
1497-1mg 1 mg 110.00$ in stock
1497-5mg 5 mg 290.00$ in stock
1497-25mg 25 mg 690.00$ in stock
1497-50mg 50 mg 1270.00$ in stock
1497-100mg 100 mg 1900.00$ in stock

Sulfo-Cyanine7 dicarboxylic acid (Сy7 analogue) is a water soluble, bifunctional NIR dye derivative bearing two carboxylic acid functional groups.

Sulfo-Cyanine7 二羧酸（Сy7 类似物）是一种水溶性双功能 NIR 染料衍生物，带有两个羧酸官能团。

Absorption and emission spectra of sulfo-Cyanine7

sulfo-Cyanine7 dicarboxylic acid

sulfo-Cyanine7 dicarboxylic acid相关产品

Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.

Sulfo-Cyanine7 NHS 酯是一种水溶性、近红外、胺反应性染料。

Cyanine5 alkyne

Cyanine5 alkyne is an alkyne dye for Click Chemistry.

Cyanine5 炔烃是一种用于 Click Chemistry 的炔烃染料。

dsGreen Gel Staining Solution, 10000×

dsGreen for staining dsDNA in gels.

dsGreen 用于在凝胶中染色 dsDNA。

General properties

Appearance: dark green solid 深绿色固体
Molecular weight: 847.09
Molecular formula: C42H51N2KO10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 carboxylic acid,Sulfo-Cyanine7 羧酸是非反应性水溶性近红外染料。

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  • Reactive dyes
  • Carboxylic acids
  • Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 carboxylic acid

标签：sulfo-Cyanine7, sulfo-Cy7, Carboxylic acids, Near infrared (NIR) imaging
货号 规格 价格 货期
15390 1 mg 110.00$ in stock
25390 5 mg 290.00$ in stock
45390 25 mg 690.00$ in stock
55390 50 mg 1270.00$ in stock
65390 100 mg 1990.00$ in stock

Sulfo-Cyanine7 carboxylic acid is non-reactive water soluble near infrared dye.

The reagent is useful as a fluorescent marker in NIR range when attachment to other molecules is not desired. It has high hydrophilicity and aqueous solubility, improved quantum yield in NIR range, and very high molar extinction coefficient.

This reagent contains free carboxylic acid function. Pre-activated activated ester – sulfo-Cyanine7 NHS ester is also available.

Sulfo-Cyanine7 羧酸是非反应性水溶性近红外染料。

当不需要与其他分子结合时，该试剂可用作 NIR 范围内的荧光标记。 它具有高亲水性和水溶性，在近红外范围内提高了量子产率，并且具有非常高的摩尔消光系数。

该试剂含有游离羧酸功能。 也可提供预活化的活化酯 – 磺基花青7 NHS 酯。

Sulfo-Cyanine7 absorbance and emission spectra

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 carboxylic acid相关产品

Sulfo-Cyanine7.5 azide

Sulfo-Cyanine7.5 is a very hydrophilic and water soluble dye for the near infrared region. The absorption and emission spectra of the dye are similar to indocyanine green (ICG), but sulfo-Cyanine7.5 has a much higher fluorescence quantum yield. The dye is a great choice for near infrared in vivo imaging. This is an azide for Click chemistry.

Sulfo-Cyanine7.5 是一种用于近红外区域的亲水性和水溶性染料。 该染料的吸收和发射光谱与吲哚菁绿 (ICG) 相似，但磺基-Cyanine7.5 具有更高的荧光量子产率。 该染料是近红外体内成像的绝佳选择。 这是点击化学的叠氮化物。

Cyanine5.5 carboxylic acid

Cy5.5 unactivated free acid cyanine dye.

Cy5.5 未活化的游离酸性花青染料。

Sulfo-Cyanine7 azide

Water-soluble NIR fluorescent dye azide for Click chemistry

用于点击化学的水溶性 NIR 荧光染料叠氮化物

General properties

Appearance: dark green powder 深绿色粉末
Molecular weight: 746.97
CAS number: 2104632-29-1 (inner salt); 2104632-30-4 (sodium salt)
Molecular formula: C37H43N2KO8S2
Solubility: Soluble in water, DMF, DMSO (0.10 M = 76 g/L). Practically insoluble in non-polar organic solvents.
Quality control: NMR 1H, HPLC (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04

相关文献：

  1. Matoori, S.; Mooney, D.J. Near-Infrared Fluorescence Hydrogen Peroxide Assay for Versatile Metabolite Biosensing in Whole Blood. Small, 2020, 16(20), 2000369. doi: 10.1002/smll.202000369
  2. Pfister, J.; Summer, D.; Petrik, M.; Khoylou, M.; Lichius, A.; Kaeopookum, P.; Kochinke, L.; Orasch, T.; Haas, H.; Decristoforo, C. Hybrid Imaging of Aspergillus fumigatus Pulmonary Infection with Fluorescent, 68Ga-Labelled Siderophores. Biomolecules, 2020, 10, 168. doi: 10.3390/biom10020168
  3. Luthman, A.S. Wide-Field fHSI with a Linescan SRDA. In: Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging (Springer Thesis), 2018, 51–85. doi: 10.1007/978-3-319-98255-7_3
  4. Luthman, A.S.; Dumitru, S.; Quiros-Gonzalez, I.; Joseph, J.; Bohndiek, S.E. Fluorescence hyperspectral imaging (fHSI) using a spectrally resolved detector array. Journal of Biophotonics, 2017, 10(6–7), 840–853. doi: 10.1002/jbio.201600304
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine7 maleimide

Sulfo-Cyanine7 maleimide,一种带有马来酰亚胺基团的活性染料，用于标记蛋白质和其他生物分子的硫醇基团，是 Cy7® 马来酰亚胺的类似物。

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  • Sulfo-Cyanine7 maleimide

Sulfo-Cyanine7 maleimide

标签：sulfo-Cyanine7, Cy7,Maleimides, Near infrared (NIR) imaging
货号 规格 价格 货期
15380 1 mg 110.00$ in stock
25380 5 mg 290.00$ in stock
45380 25 mg 690.00$ in stock
55380 50 mg 1270.00$ in stock
65380 100 mg 1990.00$ in stock

A reactive dye with a maleimide group for the labeling of thiol groups of proteins and other biomolecules, an analog of Cy7® maleimide.

Sulfo-Cyanine7 is a near infrared fluorophore which is hydrophilic and contains a rigidizing ring in its heptamethine chain that improves its photophysical properties. A great dye for various applications, including NIR imaging.

一种带有马来酰亚胺基团的活性染料，用于标记蛋白质和其他生物分子的硫醇基团，是 Cy7® 马来酰亚胺的类似物。

Sulfo-Cyanine7 是一种近红外荧光团，具有亲水性，在其七甲碱链中包含一个刚性环，可改善其光物理性质。 适用于各种应用的出色染料，包括 NIR 成像。

Sulfo-Cyanine7 maleimide absorbance and emission spectra

Sulfo-Cyanine7 maleimide

Sulfo-Cyanine7 maleimide相关产品

AF488 NHS ester

AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.

AF488 NHS 酯，一种用于绿色通道的明亮、光稳定性和亲水性荧光染料。

Cyanine7.5 maleimide

Cyanine7.5 maleimide is a thiol reactive near infrared dye.

Cy7.5 马来酰亚胺是一种硫醇活性近红外染料。

Cyanine5 amine

Cyanine5 amine functionalized dye for the coupling with activated esters and epoxides.

用于与活化酯和环氧化物偶联的花青5 胺官能化染料。

General properties

Appearance: dark green powder 深绿色粉末
Mass spec M+ increment: 868.2
Molecular weight: 869.10
CAS number: 2183440-60-8 (potassium salt), 2183440-59-5 (inner salt)
Molecular formula: C43H49KN4O9S2
IUPAC name: 1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt
Solubility: Good in water, DMF, DMSO. Low in non-polar organic solvents.
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04

相关文献：

  1. Lee, J.; Kang, Y.K.; Oh, E.; Jeong, J.; Im, S.H.; Kim, D.K.; Lee, H.; Kim, S.-G.; Jung, K.; Chung, H.J. Nano-assembly of a Chemically Tailored Cas9 Ribonucleoprotein for In Vivo Gene Editing and Cancer Immunotherapy. Chemistry of Materials, in press. doi: 10.1021/acs.chemmater.1c02844
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine7 alkyne

Sulfo-Cyanine7 alkyne,Sulfo-Cy7 炔烃是用于铜催化点击化学反应的磺化末端炔烃，具有显着的水溶性，可在水环境中与叠氮化物共轭。

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  • Sulfo-Cyanine7 alkyne

Sulfo-Cyanine7 alkyne

标签：sulfo-Cyanine7, Cy7,Alkynes
货号 规格 价格 货期
A53B0 1 mg 110.00$ in stock
B53B0 5 mg 290.00$ in stock
C53B0 10 mg 470.00$ in stock
D53B0 25 mg 690.00$ in stock
E53B0 50 mg 1270.00$ in stock
F53B0 100 mg 1990.00$ in stock

Sulfo-Cyanine7 alkyne is a sulfonated terminal alkyne for copper-catalyzed Click chemistry reaction, which possesses significant aqueous solubility, and can be conjugated with azides in aqueous environment.

Sulfo-Cy7 炔烃是用于铜催化点击化学反应的磺化末端炔烃，具有显着的水溶性，可在水环境中与叠氮化物共轭。

Sulfo-Cyanine7 absorbance and emission spectra

Sulfo-Cyanine7 alkyne

Sulfo-Cyanine7 alkyne相关产品

Cyanine3 alkyne

Cyanine3 alkyne is a fluorescent dye alkyne for Click Chemistry.

Cy3 炔烃是一种用于 Click Chemistry 的荧光染料炔烃。

Cyanine5.5 amine

Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.

Cy5.5 的氨基衍生物，一种发射远红色荧光的花青染料。

dsGreen for Real-Time PCR, 100×

Stock solution of dsGreen for Real-Time PCR, 100×

用于实时 PCR 的 dsGreen 储备溶液，100×

General properties

Appearance: dark green powder 深绿色粉末
Mass spec M+ increment: 745.3
Molecular weight: 784.04
CAS number: 2183440-56-2
Molecular formula: C40H46N3KO7S2
Solubility: good in DMF, DMSO, significant in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine7 azide,Cy7 叠氮化物

Sulfo-Cyanine7 azide,Cy7 叠氮化物,一种用于 Click Chemistry 的水溶性 NIR 染料叠氮化物。 Sulfo-Cyanine7 是一种花青染料，是 Cy7® 的类似物，具有改进的光物理特性。这种染料是活体生物近红外成像研究的完美选择。

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  • Reactive dyes
  • Dye azides
  • Sulfo-Cyanine7 azide

Sulfo-Cyanine7 azide

标签：sulfo-Cyanine7, Cy7,Azides, Near infrared (NIR) imaging
货号 规格 价格 货期
A5330 1 mg 110.00$ in stock
B5330 5 mg 290.00$ in stock
C5330 10 mg 470.00$ in stock
D5330 25 mg 690.00$ in stock
E5330 50 mg 1270.00$ in stock
F5330 100 mg 1990.00$ in stock

A water-soluble NIR dye azide for Click Chemistry. Sulfo-Cyanine7 is a cyanine dye, an analog of Cy7® with improved photophysical properties.

This dye is a perfect choice for NIR imaging studies in live organisms.

一种用于 Click Chemistry 的水溶性 NIR 染料叠氮化物。 Sulfo-Cyanine7 是一种花青染料，是 Cy7® 的类似物，具有改进的光物理特性。

这种染料是活体生物近红外成像研究的完美选择。

Sulfo-Cyanine7 absorbance and emission spectra

Sulfo-Cyanine7 azide

Sulfo-Cyanine7 azide,Cy7 叠氮化物相关产品

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cy7 carboxylic acid is a non-reactive fluorescent dye which can be used as a NIR (near infrared) marker.

Sulfo-Cy7 羧酸是一种非反应性荧光染料，可用作 NIR（近红外）标记。

Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.

Cy5.5 NHS 是远红/近红外胺活性染料。

Cyanine7 hydrazide

Carbonyl reactive NIR fluorophore for the labeling of aldehydes, ketones, including glycoproteins subjected to periodate oxidation.

羰基反应性 NIR 荧光团，用于标记醛、酮，包括经过高碘酸盐氧化的糖蛋白。

General properties

Appearance: dark green powder 深绿色粉末
Molecular weight: 829.08
Molecular formula: C40H49N6KO7S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04
Cy® is a trademark of GE Healthcare.