标签： sulfo-Cyanine3

lumiprobe -Cyanine3B carboxylic acid,2321-1mg

lumiprobe -Cyanine3B carboxylic acid,2321-1mg,Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

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Cyanine3B carboxylic acid

Cyanine3, Carboxylic acids, sulfo-Cyanine3,Cy3
Cat. # Quantity Price Lead time
2321-1mg 1 mg 110.00$ 8 days
2321-5mg 5 mg 149.00$ 8 days
2321-25mg 25 mg 690.00$ 8 days
2321-50mg 50 mg 1290.00$ 8 days
2321-100mg 100 mg 2450.00$ 8 days

General properties

Appearance: dark red powder
Molecular weight: 560.66
Molecular formula: C31H32N2O6S
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Cyanine3B carboxylic acid

Lumiprobe 活性荧光染料分类简介

Near infrared (NIR) imaging, BDP 558/568, AF568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Protein labeling, AF488, NHS esters, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, Cross linking reagents, AF594, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年起，公司开始生产并销售生命科学研究和诊断学应用的优质化学药品。主要产品有：活性染料（ReactiveDye）和SYBRGreen I染料，定制合成荧光双标记引物。产品主要应用：点击化学（CLickChemistry）、蛋白质组学研究中的双向荧光差异凝胶电泳（2DDIGE）和实时荧光定量PCR（ReaLtimePCR）。Lumiprobe是通过ISO 9001认证的生命科学应用标准和定制试剂制造商。

荧光染料又称为荧光探针, 因具备检测速度快、重复性好、用样量少、无辐射等优点，成为大家广泛使用的荧光标示剂。同时也是细胞生物学等科学研究中不可或缺的重要工具，利用荧光探针可测定RNA和DNA的结构、研究DNA碱基损伤修复、辨别蛋白质分子中氨基的状态和蛋白质分子的活性区, 检测pmol级的蛋白质 ,区分不同构象的核酸以及有关药物的化学反应活性。荧光免疫分析采用时间-分辨技术可用于许多蛋白质、激素、病毒抗原及DNA杂交体的分析。激光诱导荧光光谱在活细胞、活体体液、DNA碱基序列和细菌原体的鉴定、恶性肿瘤的早期诊断和治疗中起到了重要的作用。

由于大多数荧光染料不具备选择性，被固载在生物活性载体的荧光染料，可利用生物活性物质之间的选择性（抗原抗体，生物素链霉亲和素），或者通过染料分子用适当的修饰官能团，通过官能团之间的相互反应，实现对目标物质的靶向性标记，下面我们就来给大家介绍常见的几类活性荧光染料：

活性荧光素及其衍生物广泛用于共价标记生物分子，例如抗体，碳水化合物，核酸，蛋白质，肽和寡核苷酸。各活化官能团对应的反应基团如下：

名称用途
琥珀酰亚胺酯（Succinimidyl esters）/ NHS酯Near infrared (NIR) imaging, BDP 558/568, AF568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Protein labeling, AF488, NHS esters, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, Cross linking reagents, AF594, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343
荧光染料 NHS 酯是具有活化酯基的胺活性染料。
马来酰亚胺（Maleimides）Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, Maleimides, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5
染料马来酰亚胺是硫醇反应性荧光染料，用于标记蛋白质中的巯基（硫醇）基团。
叠氮化物(Azides)Near infrared (NIR) imaging, BDP 558/568, AF568, Phenylethynyl-pyrene (PEP), Cyanine3.5, Microscopy, sulfo-Cyanine3.5, AF488, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, HEX (Hexachlorofluorescein), BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Click chemistry, Cyanine7.5, Perylene, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), Azides, AF430, JOE, BDP TR, R6G, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343
用于点击化学标记的荧光染料叠氮化物。
炔烃(Alkynes) Near infrared (NIR) imaging, BDP 558/568, AF568, Microscopy, sulfo-Cyanine3.5, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, Alkynes, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, Perylene, ROX (Rhodamine X, Rhodamine 101), TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, R6G, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5
用于铜催化点击化学的荧光染料炔烃。含有末端炔基。
酰肼(Hydrazides)Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, Cyanine5, BDP 581/591, FAM (Fluorescein), BDP FL, BDP 630/650, Cyanine7.5, TAMRA (TMR, tetramethylrhodamine), Hydrazides, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene
染料酰肼是用于标记醛和酮的羰基反应性荧光染料。
羧酸(Carboxylic acids)Near infrared (NIR) imaging, BDP 558/568, Cyanine3.5, Microscopy, sulfo-Cyanine3.5, Cyanine7, sulfo-Cyanine7, sulfo-Cyanine7.5, Cyanine5, BDP 581/591, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, Carboxylic acids, AF430, BDP TR, BDP 564/570, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, Pyrene, sulfo-Cyanine5.5, Coumarin 343
荧光染料的羧酸衍生物。
可用于在碳二亚胺预活化后标记胺，也可用于醇的 Steglich 酯化。
氨基(Amines)Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, Cyanine5, BDP 581/591, Amines, FAM (Fluorescein), BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, TAMRA (TMR, tetramethylrhodamine), AF430, BDP TR, Fluorescence polarization, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, sulfo-Cyanine5.5
具有游离氨基的荧光染料，用于与各种亲电化合物如活化酯和环氧化物偶联。
四嗪（Tetrazines）Near infrared (NIR) imaging, BDP 558/568, Microscopy, Cyanine7, sulfo-Cyanine7, Copper free Click chemistry, Tetrazine, Cyanine5, BDP 581/591, FAM (Fluorescein), BDP 576/589, BDP FL, BDP TMR, BDP 630/650, sulfo-Cyanine5, Cyanine7.5, AF430, BDP TR, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, PEG3 (triethylene glycol)
四嗪是通过反向电子需求[4+2]-环加成反应与反式环辛烯、环丙烯和其他活化烯烃反应的衍生物——无铜点击化学反应的一个例子。
亚磷酰胺(Phosphoramidite)Oligonucleotide synthesis, Cyanine5, TET, HEX (Hexachlorofluorescein), Amines, PEG6 (hexaethylene glycol), FAM (Fluorescein), Alkynes, Phosphoramidite, TAMRA (TMR, tetramethylrhodamine), JOE, R6G, Cyanine5.5, Cyanine3, Pyrene, SIMA
用于固相自动化寡核苷酸合成的亚磷酰胺（亚酰胺）改性剂。
荧光亚磷酰胺，用于点击化学 DNA 修饰的亚酰胺。
环炔(Cycloalkyne)BDP 558/568, Cyanine3.5, Cyanine7, Copper free Click chemistry, Cyanine5, BDP 581/591, BDP 650/665, FAM (Fluorescein), BDP FL, BDP TMR, sulfo-Cyanine5, Cycloalkyne, ROX (Rhodamine X, Rhodamine 101), BDP TR, Cyanine5.5, BDP R6G, sulfo-Cyanine3, Cyanine3, sulfo-Cyanine5.5
用于无铜点击化学的具有环炔烃部分的染料。

sulfo-Cyanine3 DBCO

sulfo-Cyanine3 DBCO,储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。Sulfo-Cyanine3 DBCO 是一种具有环炔基团的水溶性荧光染料，用于无铜点击化学。

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sulfo-Cyanine3 DBCO

标签：Copper free Click chemistry, Cycloalkyne, sulfo-Cyanine3
货号 规格 价格 货期
113F0 1 mg 110.00$ in stock
213F0 5 mg 290.00$ in stock
413F0 25 mg 690.00$ in stock
513F0 50 mg 1270.00$ in stock
613F0 100 mg 1990.00$ in stock

Sulfo-Cyanine3 DBCO is a water soluble fluorescent dye with cycloalkyne group for copper free Click chemistry.

DBCO (dibenzocyclooctyne) is a cyclooctyne with very high reactivity towards azides, still possessing good stability.

Sulfo-Cyanine3 DBCO 是一种具有环炔基团的水溶性荧光染料，用于无铜点击化学。

DBCO（二苯并环辛炔）是一种环辛炔，对叠氮化物具有非常高的反应性，但仍具有良好的稳定性。

Absorption and emission spectra of sulfo-Cyanine3

sulfo-Cyanine3 DBCO

sulfo-Cyanine3 DBCO相关产品

Sulfo-Cyanine3 azide

Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.

用于 Click Chemistry 的水溶性磺基-Cy3 染料叠氮化物。

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5® channel.

用于 Cy5® 的硫醇活性水溶性花青染料 渠道。

Sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.

NHS 酯形式的水溶性磺化远红色磺基-Cy5.5 染料。

General properties

Appearance: red solid 红色固体
Mass spec M+ increment: 916.4
Molecular weight: 955.23
Molecular formula: C51H55N4KO8S2
Solubility: soluble in water (0.11 M = 11 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关产品

  1. Tugel, U.; Casas, M.G.; Wiltschi, B. Site-Specific Incorporation of Non-canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli. Peptide and Protein Engineering (Springer Protocols Handbooks), 2020, 267–281. doi: 10.1007/978-1-0716-0720-6_14
  2. Desai, B.J.; Gonzalez, R.L. Multiplexed genomic encoding of non-canonical amino acids for labeling large complexes. Nature Chemical Biology, 2020, 16, 1129–1135. doi: 10.1038/s41589-020-0599-5

Sulfo-Cyanine3 tetrazine

Sulfo-Cyanine3 tetrazine,四嗪-反式-环辛烯连接是迄今为止已知最快的生物偶联反应之一。

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Sulfo-Cyanine3 tetrazine

标签：Tetrazin,Copper free Click chemistr,sulfo-Cyanine3,sulfo-Cy3
货号 规格 价格 货期
113E0 1 mg 110.00$ in stock
213E0 5 mg 290.00$ in stock
313E0 10 mg 460.00$ in stock
413E0 25 mg 690.00$ in stock
513E0 50 mg 1270.00$ in stock
613E0 100 mg 1990.00$ in stock

Tetrazine – trans-cyclooctene ligation is among the fastest bioconjugation reactions known to date.

This water soluble sulfo-Cyanine3 derivative contains methyltetrazine moiety for the coupling with trans-cyclooctenes. Methyltetrazines possess optimal stability at physiological pH, while maintaining extremely high reactivity towards cyclooctenes.

The fluorophore is bright, photostable, and is also very well visible with naked eye.

四嗪-反式-环辛烯连接是迄今为止已知最快的生物偶联反应之一。

这种水溶性磺基-Cyanine3 衍生物含有甲基四嗪部分，用于与反式环辛烯偶联。 甲基四嗪在生理 pH 值下具有最佳稳定性，同时对环辛烯保持极高的反应性。

荧光团明亮、光稳定，而且肉眼也能很好地看到。

Sulfo-Cyanine3 tetrazine相关产品

FAM tetrazine, 5-isomer

FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.

FAM 四嗪（甲基四嗪）用于与反式环辛烯和环丙烯共轭。 荧光素的纯 5-异构体。

Biotin-PEG3-Azide

Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe.

Biotin azide 是一种用生物素（一种众所周知的亲和探针）进行点击化学标记的试剂。

AmdU (5-azidomethyl-2′-deoxyuridine)

Azidomethyldeoxyuridine (AmdU) is an azide containing nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain promoted Click chemistry.

叠氮甲基脱氧尿苷 (AmdU) 是一种含有核苷的叠氮化物，可通过细胞聚合酶整合到新生 DNA 中。 以这种方式连接到 DNA 上的叠氮基可以随后通过铜催化或应变促进的点击化学被炔烃或环炔烃修饰。

General properties

Appearance: red powder 红色粉末
Molecular weight: 838.05
Molecular formula: C40H44KN7O7S2
IUPAC name: Potassium (E)-2-[(E)-3-{3,3-Dimethyl-1-[6-({[p-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]methyl}amino)-6-oxohexyl]-5-(oxysulfonyl)-3H-indol-2-yl}-2-propenylidene]-1-methyl-3,3-dimethyl-5-indolinesulfonate
Solubility: soluble in water (0.43 M = 36 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine3 amine

Sulfo-Cyanine3 amine,一种带有氨基的水溶性染料，可用于与亲电试剂结合，以及用于酶促氨基转移标记。Sulfo-Cyanine3 是 Cy3® 的磺化类似物，可与各种荧光测量设备兼容。 该染料具有高度的光稳定性。 它也很容易被肉眼检测到。

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  • Sulfo-Cyanine3 amine

Sulfo-Cyanine3 amine

标签：Amines, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
113C0 1 mg 110.00$ in stock
213C0 5 mg 290.00$ in stock
413C0 25 mg 690.00$ in stock
513C0 50 mg 1270.00$ in stock
613C0 100 mg 1990.00$ in stock

Sulfo-Cyanine3 amineA water soluble dye with amino group, useful for the conjugation with electrophiles, and for enzymatic transamination labeling.

Sulfo-Cyanine3 is a sulfonated analog of Cy3®, which is compatible with various fluorescence measuring equipment. The dye is highly photostable. It is also easily detectable by naked eye.

一种带有氨基的水溶性染料，可用于与亲电试剂结合，以及用于酶促氨基转移标记。

Sulfo-Cyanine3 是 Cy3® 的磺化类似物，可与各种荧光测量设备兼容。 该染料具有高度的光稳定性。 它也很容易被肉眼检测到。

Sulfo-Cy3 absorbance and emission spectra

Sulfo-Cyanine3 amine

Sulfo-Cyanine3 amine相关产品

Cyanine5 DBCO

Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper free Click chemistry.

用于无铜点击化学的具有二苯并环辛炔 (DBCO) 官能团的 Cy5 染料。

BDP 558/568 amine

BDP 558/568 is a borondipyrromethene dye with yellow/orange emission. This amine derivative can be conjugated by means of reactions with various electrophiles.

BDP 558/568 是一种具有黄色/橙色发射光的硼二吡咯亚甲基染料。 这种胺衍生物可以通过与各种亲电子试剂的反应来缀合。

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5® channel.

用于 Cy5® 的硫醇活性水溶性花青染料 渠道。

General properties

Appearance: dark red solid 暗红色固体
Molecular weight: 714.94
CAS number: 2183440-43-7
Molecular formula: C36H50N4O7S2
IUPAC name: 3H-​Indolium, 1-​[6-​[(6-​aminohexyl)​amino]​-​6-​oxohexyl]​-​2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​3,​3-​dimethyl-​5-​sulfo-​, inner salt
Solubility: well soluble in water (0.49 M = 350 g/L), alcohols, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献：

  1. Bos, I.; Timmerman, M.; Sprakel, J. FRET-Based Determination of the Exchange Dynamics of Complex Coacervate Core Micelles. Macromolecules, 2021, 54(1), 398–411. doi: 10.1021/acs.macromol.0c02387
  2. Zhang, Q.; Scigliano, A.; Biver, T.; Pucci, A.; Swager, T.M. Interfacial Bioconjugation on Emulsion Droplet for Biosensors. Bioorganic & Medicinal Chemistry, 2018, 26(19), 5307–5313. doi: 10.1016/j.bmc.2018.04.020
  3. Sobotzki, N.; Schafroth, M.A.; Rudnicka, A.; Koetemann, A.; Marty, F.; Goetze, S.; Yamauchi, Y.; Carreira, E.M.; Wollscheid, B. HATRIC-based identification of receptors for orphan ligands. Nature Communications, 2018, 9, 1519. doi: 10.1038/s41467-018-03936-z

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 carboxylic acid,水溶性磺基-Cy3 染料，游离未活化的单官能羧酸。 该试剂可用作 Cy3® 检测通道的参考荧光团，在其他磺基-Cyanine3 标记产品的实验中用作对照。 羧酸也可以用碳二亚胺活化。

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  • Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 carboxylic acid

标签：Carboxylic acids, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
11390 1 mg 110.00$ in stock
21390 5 mg 210.00$ in stock
41390 25 mg 410.00$ in stock
51390 50 mg 695.00$ in stock
61390 100 mg 1190.00$ in stock

Water soluble sulfo-Cyanine3 dye, free unactivated monofunctional carboxylic acid. This reagent can be used as a reference fluorophore for Cy3® detection channel, as a control in experiments with other sulfo-Cyanine3 labeled products. Carboxylic acid can be also activated with carbodiimides.

Absorbance and emission spectra are identical with Cy3® fluorophore.

水溶性磺基-Cyanine3 染料，游离未活化的单官能羧酸。 该试剂可用作 Cy3® 检测通道的参考荧光团，在其他磺基-Cyanine3 标记产品的实验中用作对照。 羧酸也可以用碳二亚胺活化。

吸光度和发射光谱与 Cy3® 荧光团相同。

Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 carboxylic acid相关产品

AF568 azide, 6-isomer

AF 568 derivative designed for different imaging applications

AF 568 衍生产品专为不同的成像应用而设计

THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.

THPTA 是一种用于 Cu(I) 催化点击化学的水溶性配体。 配体将铜稳定在其 Cu(I) 氧化态。 由于高水溶性，与该配体的反应不需要有机助溶剂。

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团（氨基染料）。 它可以与 NHS 酯、羧基（活化后）和环氧化物结合。

General properties

Appearance: dark red crystals 暗红色晶体
Molecular weight: 654.84
CAS number: 1121756-11-3 (inner salt); 1941997-61-0 (sodium salt)
Molecular formula: C30H35N2KO8S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​(5-​carboxypentyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt, potassium salt
Solubility: Well soluble in water, DMF, DMSO (0.55 M = 360 g/L). Practically insoluble in non-polar organic solvents.
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献：

  1. Vallmitjana, A.; Torrado, B.; Dvornikov, A.; Ranjit, S.; Gratton, E. Blind Resolution of Lifetime Components in Individual Pixels of Fluorescence Lifetime Images Using the Phasor Approach. Journal of Physical Chemistry B, 2020, 124(45), 10126–10137. doi: 10.1021/acs.jpcb.0c06946
  2. Cho, U.; Riordan, D.P.; Ciepla, P.; Kocherlakota, K.S.; Chen, J.K.; Harbury, P.B. Ultrasensitive optical imaging with lanthanide lumiphores. Nature Chemical Biology, 2018, 14(1), 15–21. doi: 10.1038/nchembio.2513
  3. Spears, B.R.; Marin, M.A.; Chaker, A.N.; Lampley, M.W.; Harth, E. Precise Microscale Polymeric Networks through Piezoelectronic Inkjet Printing. ACS Biomaterials Science & Engineering, 2016, 2(8), 1265–1272. doi: 10.1021/acsbiomaterials.6b00175
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide,Sulfo-Cy3 是 Cy3 染料的硫酸化衍生物，由于其结构中有两个带负电荷的磺基，因此可很好地溶于水。根据其光谱特性，这种橙黄色荧光染料是 Cy™3 的类似物。

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  • Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide

标签：Hydrazides, Microscopy, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
11370 1 mg 110.00$ in stock
21370 5 mg 290.00$ in stock
41370 25 mg 690.00$ in stock
51370 50 mg 1270.00$ in stock
61370 100 mg 1990.00$ in stock

Sulfo-Cyanine3 is a sulfated derivative of Cyanine3 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this yellow-orange fluorescent dye is an analog of Cy™3.

Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with Sulfo-Cyanine3 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with Sulfo-Cyanine3 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

Sulfo-Cy3 是 Cy3 染料的硫酸化衍生物，由于其结构中有两个带负电荷的磺基，因此可很好地溶于水。根据其光谱特性，这种橙黄色荧光染料是 Cy™3 的类似物。

酰肼有效地与醛和酮反应生成腙，因此该化合物可用于与生物分子的羰基衍生物缀合。

反应在水性条件下进行，这在处理抗体和蛋白质时很重要。糖基化蛋白质和抗体中的糖中的 Cys-二醇基团可被氧化成二醛，蛋白质中的半胱氨酸可被酶转化为甲酰基甘油（即与磺基花青 3 酰肼结合的反应基团）。蛋白质和肽中的天冬氨酸和谷氨酸的羧基也可以在活化剂存在的情况下与磺基花青 3 酰肼结合：碳二亚胺 (EDAC) 或甲基吗啉 (DMTMM) 衍生物。

Absorption and emission spectra of sulfo-Cyanine3

Sulfo-Cyanine3 hydrazide

Sulfo-Cyanine3 hydrazide相关产品

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cy3 SE 活化酯。

Cyanine3 hydrazide

Cyanine3 hydrazide is a carbonyl-reactive dye for Cy3 channel.

Cy3 hydrazide 是 Cy3 通道的羰基反应性染料。

General properties

Appearance: dark red solid 暗红色固体
Molecular weight: 630.78
CAS number: 2144762-62-7
Molecular formula: C30H38N4O7S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​(6-​hydrazinyl-​6-​oxohexyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt
Solubility: soluble in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (90%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
Cy™ is a registered trademark of Cytiva in some countries.

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide,Sulfo-Cy3 马来酰亚胺是一种水溶性硫醇活性染料，可用于标记亲水性磺基-Cyanine3 荧光团。 这是 Cy3® 马来酰亚胺的类似物。

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  • Dye maleimides
  • Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide

标签：Maleimides, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
11380 1 mg 110.00$ in stock
21380 5 mg 290.00$ in stock
41380 25 mg 690.00$ 5 days
51380 50 mg 1270.00$ in stock
61380 100 mg 1990.00$ in stock

Sulfo-Cyanine3 maleimide is a water soluble, thiol reactive dye for the labeling with hydrophilic sulfo-Cyanine3 fluorophore. This is an analog of Cy3® maleimide.

This product is recommended for the labeling of antibodies and other labile proteins in mild, purely aqueous conditions. The dye is water soluble and does not require use of organic co-solvent.

A non-sulfonated Cyanine3 maleimide is available.

Sulfo-Cyanine3 马来酰亚胺是一种水溶性硫醇活性染料，可用于标记亲水性磺基-Cyanine3 荧光团。 这是 Cy3® 马来酰亚胺的类似物。

该产品推荐用于在温和的纯水性条件下标记抗体和其他不稳定的蛋白质。 该染料是水溶性的，不需要使用有机助溶剂。

可使用非磺化的 Cyanine3 马来酰亚胺。

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 maleimide相关产品

Cyanine3 maleimide

Cyanine3 maleimide is a thiol mono reactive dye.

Cy3 马来酰亚胺是一种硫醇单活性染料。

Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.

NIR荧光染料Cyanine7.5的胺反应性NHS酯。

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cyanine3 SE 活化酯。

General properties

Appearance: red powder 红色粉末
Molecular weight: 776.96
CAS number: 1656990-68-9
Molecular formula: C36H41KN4O9S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt, potassium salt
Solubility: soluble in water (0.39 M = 30 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献：

  1. Ast, J.; Novak, A.N.; Podewin, T.; Fine, N.H.F.; Jones, B.; Tomas, A.; Birke, R.; Roßmann, K.; Mathes, B:; Eichhorst, J.; Lehmann, M.; Linnemann, A.K.; Hodson, D.J.; Broichhagen, J. An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo. ChemRxiv, preprint. doi: 10.33774/chemrxiv-2021-7rngq
  2. Mohale, M.; Gundampati, R.K.; Thallapuranam, S.; Heyes, C.D. Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent dyes in the flexible N-Terminal region and a rigid β-turn region. Analytical Biochemistry, 2022, 640, 114524. doi: 10.1016/j.ab.2021.114524
  3. Bauer, B.W.; Davidson, I.F.; Canena, D.; Wutz, G.; Tang, W.; Litos, G.; Horn, S.; Hinterdorfer, P.; Peters, J.-M. Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism. Cell, 2021, 184(21), 5448–5464.e22. doi: 10.1016/j.cell.2021.09.016
  4. Deckard, C.E.; Sczepanski, J.T. Reversible chromatin condensation by the DNA repair and demethylation factor thymine DNA glycosylase. Nucleic Acids Research, 2021, 49(5), 2450–2459. doi: 10.1093/nar/gkab040
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine3 alkyne

Sulfo-Cyanine3 alkyne,用于铜催化 Click Chemistry 的水溶性炔烃染料，是 Cy3® 炔烃的类似物。Sulfo-Cyanine3 是一种荧光团，与各种荧光扫描仪、成像仪、显微镜和其他仪器兼容。 它是一种明亮且光稳定的染料，在凝胶中也很容易通过肉眼检测到少量（<1 nmol）。

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  • Dye alkynes
  • Sulfo-Cyanine3 alkyne

Sulfo-Cyanine3 alkyne

标签：Alkynes, Microscopy, sulfo-Cyanine3,Cy3.5
货号 规格 价格 货期
A13B0 1 mg 110.00$ in stock 货号 规格 价格 货期
B13B0 5 mg 290.00$ in stock
C13B0 10 mg 470.00$ in stock
D13B0 25 mg 690.00$ in stock
E13B0 50 mg 1270.00$ in stock
F13B0 100 mg 1990.00$ in stock

A water soluble alkyne dye for copper catalyzed Click Chemistry, an analog of Cy3® alkyne.

Sulfo-Cyanine3 is a fluorophore which is compatible with a wide range of fluorescent scanners, imagers, microscopes, and other instrumentation. It is a bright and photostable dye, which is also easily detected in gels by naked eye in low amounts (<1 nmol).

用于铜催化 Click Chemistry 的水溶性炔烃染料，是 Cy3® 炔烃的类似物。

Sulfo-Cyanine3 是一种荧光团，与各种荧光扫描仪、成像仪、显微镜和其他仪器兼容。 它是一种明亮且光稳定的染料，在凝胶中也很容易通过肉眼检测到少量（<1 nmol）。

Absorbance and emission spectra of Sulfo-Cyanine3 dye

Sulfo-Cyanine3 alkyne

Sulfo-Cyanine3 alkyne相关产品

THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.

THPTA 是一种用于 Cu(I) 催化点击化学的水溶性配体。 配体将铜稳定在其 Cu(I) 氧化态。 由于高水溶性，与该配体的反应不需要有机助溶剂。

Sulfo-Cyanine3 tetrazine

Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.

水溶性磺基-Cy3 染料四嗪衍生物，用于用反式环辛烯进行 TCO 连接标记。

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5&reg; channel.

用于 Cy5&reg; 的硫醇活性水溶性花青染料 渠道。

General properties

Appearance: dark red powder 暗红色粉末
Mass spec M+ increment: 653.2
Molecular weight: 691.90
CAS number: 2055138-87-7 (inner salt); 2055138-88-8 (sodium salt)
Molecular formula: C33H38KN3O7S2
Solubility: soluble in water (0.57 M = 40 g/L), DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献:

  1. Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal Imaging and Pharmacokinetic of Fluorescent Compounds in Zebrafish Eleuthero-Embryos After Different Routes of Administration. Research Square, preprint. doi: 10.21203/rs.3.rs-237483/v1
  2. Tao, Y.; Xie, J.; Zhong, Q.; Wang, Y.; Zhang, S.; Luo, F.; Wen, F.; Xie, J.; Zhao, J.; Sun, X.; Long, H.; Ma, J.; Zhang, Q.; Long, J.; Fang, X.; Lu, Y.; Li, D.; Li, M.; Zhu, J.; Sun, B.; Li, G.; Diao, J.; Liu, C. A novel partially open state of {SHP}2 points to a “multiple gear” regulation mechanism. Journal of Biological Chemistry, 2021, 296, 100538. doi: 10.1016/j.jbc.2021.100538
  3. Tugel, U.; Casas, M.G.; Wiltschi, B. Site-Specific Incorporation of Non-canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli. Peptide and Protein Engineering (Springer Protocols Handbooks), 2020, 267–281. doi: 10.1007/978-1-0716-0720-6_14
  4. Sokolova, V.; Nzou, G.; van der Meer, S.B.; Ruks, T.; Heggen, M.; Loza, K.; Hagemann, N.; Murke, F.; Giebel, B.; Hermann, D.M.; Atala, A.J.; Epple, M. Ultrasmall gold nanoparticles (2 nm) can penetrate and enter cell nuclei in an in vitro 3D brain spheroid model. Acta Biomaterialia, 2020, 111, 349–362. doi: 10.1016/j.actbio.2020.04.023
Cy® is a trademark of GE Healthcare.

Sulfo-Cyanine3 azide

Sulfo-Cyanine3 azide,Sulfo-Cyanine3 azide 是一种用于 Click Chemistry 的水溶性染料叠氮化物。 染料的吸光度和发射与 Cy3® 荧光团相同。

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  • Dye azides
  • Sulfo-Cyanine3 azide

Sulfo-Cyanine3 azide

标签：Azides, Microscopy, sulfo-Cyanine3,Cy3
货号 规格 价格 货期
A1330 1 mg 110.00$ in stock
B1330 5 mg 290.00$ in stock
C1330 10 mg 470.00$ in stock
D1330 25 mg 690.00$ in stock
E1330 50 mg 1270.00$ in stock
F1330 100 mg 1990.00$ in stock

Sulfo-Cyanine3 azide is a water-soluble dye azide for Click Chemistry. Absorbance and emission of the dye are identical to Cy3® fluorophore.

Brightness and photostability of fluorophore, together with high water solubility, makes this reagent an ideal choice for Click chemistry labeling of various molecules in aqueous phase.

Click reaction can be carried out in pure water without need to use organic co-solvent. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects.

Sulfo-Cyanine3 azide 是一种用于 Click Chemistry 的水溶性染料叠氮化物。 染料的吸光度和发射与 Cy3® 荧光团相同。

荧光团的亮度和光稳定性以及高水溶性使该试剂成为水相中各种分子的点击化学标记的理想选择。

点击反应可在纯水中进行，无需使用有机助溶剂。 该试剂设计用于标记蛋白质等敏感分子，甚至是完整的生物对象。

Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 azide

Sulfo-Cyanine3 azide相关产品

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团（氨基染料）。 它可以与 NHS 酯、羧基（活化后）和环氧化物结合。

Pico488 dsDNA quantification reagent, 200x solution in DMSO

Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.

Pico488 是一种染料，用于在存在 RNA、ssDNA 和其他杂质的情况下对 dsDNA 进行基于荧光的精确定量。 该染料在荧光素通道中显示出明亮的荧光，在低 pg/mL 至高 ng/mL 范围内对 DNA 浓度提供线性响应。

Cyanine3 maleimide

Cyanine3 maleimide is a thiol mono reactive dye.

Cy3 马来酰亚胺是一种硫醇单活性染料。

General properties

Appearance: dark red crystals 暗红色晶体
Molecular weight: 736.94
CAS number: 2055138-89-9 (sodium salt), 1658416-54-6
Molecular formula: C33H41N6KO7S2
Solubility: soluble in water (0.62 M = 46 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

相关文献：

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