标签： sulfo-Cy5

Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP,Sulfo-Cy5 dUTP（脱氧尿苷三磷酸）是一种修饰的三磷酸核苷，用于 DNA 的酶标记。 三磷酸盐是 Cy5-dUTP 的类似物。 然而，与直接用染料修饰的氨基烯丙基 dUTP 不同，这种三磷酸盐含有更长的连接臂，可提高其被常用聚合酶（如 Taq 聚合酶）掺入的效率。

  • Products
  • DNA
  • Modified triphosphates
  • Sulfo-Cyanine5 dUTP

Sulfo-Cyanine5 dUTP

标签：sulfo-Cyanine5, PCR and qPCR, Triphosphates
货号 规格 价格 货期
1510-50nmol 50 nmol 290.00$ in stock
1510-250nmol 250 nmol 950.00$ in stock

 

Sulfo-Cyanine5 dUTP (deoxyuridine triphosphate) is a modified nucleoside triphosphate for the enzymatic labeling of DNA. The triphosphate is an analog of Cy5-dUTP. However, unlike aminoallyl dUTP directly modified with dye, this triphosphate contains a longer linker arm that increases the efficiency of its incorporation by usual polymerases, like Taq polymerase.

Sulfo-Cyanine5 is a bright far red emitting dye for Cy5 channel.

The triphosphate is supplied in lyophilized form of lithium salt, ready to use.

Sulfo-Cy5 dUTP（脱氧尿苷三磷酸）是一种修饰的三磷酸核苷，用于 DNA 的酶标记。 三磷酸盐是 Cy5-dUTP 的类似物。 然而，与直接用染料修饰的氨基烯丙基 dUTP 不同，这种三磷酸盐含有更长的连接臂，可提高其被常用聚合酶（如 Taq 聚合酶）掺入的效率。

Sulfo-Cyanine5 是一种用于 Cy5 通道的明亮远红色发射染料。

三磷酸盐以锂盐的冻干形式提供，随时可用。

Absorption and emission spectra of sulfo-Cyanine5

Sulfo-Cyanine5 dUTP

Sulfo-Cy5 dUTP相关产品

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.

用于氨基生物分子标记的水溶性磺基-Cyanine3 SE 活化酯。

Sulfo-Cyanine3 dUTP

Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3. The nucleotide contains a long linker that allows to incorporate the dye efficiently into DNA strand by various enzymes including Taq polymerase.

用于用磺基花青标记 DNA 的荧光 dNTP。 核苷酸包含一个长接头，允许通过包括 Taq 聚合酶在内的各种酶将染料有效地掺入 DNA 链中。

Sulfo-Cyanine5.5 dUTP

Modified deoxy triphosphate containing sulfo-Cyanine5.5 (aka Cy5.5) dye for enzymatic labeling of DNA microarrays, as well as PCR amplicons, and reverse transcripts.

含有磺基-Cyanine5.5（又名 Cy5.5）染料的改性脱氧三磷酸，用于 DNA 微阵列以及 PCR 扩增子和逆转录物的酶标记。

General properties

Appearance: blue solid / solution 蓝色固体/溶液
Molecular weight: 1275.89
Molecular formula: C49H66N6Li4O22P3S2
Solubility: good in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下和过度冻融循环。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Sulfo-Cyanine5-PEG3-Biotin

Sulfo-Cyanine5-PEG3-Biotin,Sulfo-Cy5 是一种远红荧光团，广泛用于生物分子标记，最大激发波长为 646 nm，最大发射波长为 662 nm。激发波长高于 600 nm 且发射波长超过 650 nm 的远红荧光标签对成像技术很有价值，因为这些波长的背景自发荧光较低。此外，远红荧光标签可以与近红、橙、绿、蓝标签同时成像，有利于多色成像。

  • Products
  • Miscellaneous reagents
  • Auxiliary reagents
  • Sulfo-Cyanine5-PEG3-Biotin

Sulfo-Cyanine5-PEG3-Biotin

标签：sulfo-Cy5,Auxiliary reagents
Cat. # Quantity Price Lead time
2449-1mg 1 mg 120.00$ in stock

2449-5mg 5 mg 290.00$ in stock

Sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.

Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.

Sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.

The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.

Sulfo-Cyanine5 是一种远红荧光团，广泛用于生物分子标记，最大激发波长为 646 nm，最大发射波长为 662 nm。激发波长高于 600 nm 且发射波长超过 650 nm 的远红荧光标签对成像技术很有价值，因为这些波长的背景自发荧光较低。此外，远红荧光标签可以与近红、橙、绿、蓝标签同时成像，有利于多色成像。

众所周知，生物素或水溶性维生素 H 不仅在各种关键的代谢细胞反应中发挥重要作用，而且还因其对抗生物素蛋白（一种蛋清的糖蛋白）具有极高的亲和力。抗生物素蛋白、链霉抗生物素蛋白（抗生物素蛋白的细菌类似物）和中性抗生物素蛋白（去糖基化的抗生物素蛋白）以高亲和力与生物素非协同结合。因此，磺基-Cyanine5 生物素偶联物可用于检测和量化不同来源样品中抗生物素蛋白、链霉抗生物素蛋白和中性抗生物素蛋白的生物素结合位点。

Sulfo-Cyanine5 生物素偶联物是一种水溶性试剂，其荧光在 pH 4 到 pH 10 之间不受 pH 影响。生物素部分和荧光标签之间的灵活 PEG3 接头可与抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白结合，而不会出现空间问题。

磺基-Cyanine5 生物素偶联物的主要应用包括成像（例如用于蛋白质印迹、免疫测定、细胞和组织化学、流式细胞术的一抗和二抗标记）、亲和力和解离常数测量、基于链霉亲和素的传感器等。

Absorption and emission spectra of sulfo-Cyanine5

Sulfo-Cyanine5-PEG3-Biotin

Sulfo-Cyanine5-PEG3-Biotin相关产品

Sulfo-Cyanine5 tetrazine

Water soluble Cyanine dye tetrazine derivative for TCO ligation with trans-cyclooctenes and other strained olefines.

水溶性花青染料四嗪衍生物，用于与反式环辛烯和其他应变烯烃的 TCO 连接。

dsGreen Gel Staining Solution, 10000×

dsGreen for staining dsDNA in gels.

dsGreen 用于在凝胶中染色 dsDNA。

Sulfo-Cyanine3 azide

Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.

用于 Click Chemistry 的水溶性磺基-Cy3 染料叠氮化物。

General properties

Appearance: dark blue solid 深蓝色固体
Molecular weight: 1037.36
Molecular formula: C48H65N6KO11S3
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

sulfo-Cyanine5 DBCO

sulfo-Cyanine5 DBCO,Copper free Click chemistry reaction between strained cycloalkynes (cyclooctynes), and azides, is a very fast and robust reaction. It can be used for a fast labeling with fluorescent dyes. This reagent is a derivative of water soluble sulfo-Cyanine5 dye which emits in red channel. It is useful for the labeling of biomolecules in aqueous media.

  • Products
  • Reactive dyes
  • Cycloalkyne dyes
  • sulfo-Cyanine5 DBCO

sulfo-Cyanine5 DBCO

标签：sulfo-Cy5, Copper free Click chemistry, Cycloalkyne
货号 规格 价格 货期
133F0 1 mg 110.00$ in stock
233F0 5 mg 290.00$ in stock
433F0 25 mg 690.00$ in stock
533F0 50 mg 1270.00$ in stock
633F0 100 mg 1990.00$ in stock

Copper free Click chemistry reaction between strained cycloalkynes (cyclooctynes), and azides, is a very fast and robust reaction. It can be used for a fast labeling with fluorescent dyes. This reagent is a derivative of water soluble sulfo-Cyanine5 dye which emits in red channel. It is useful for the labeling of biomolecules in aqueous media.

紧张的环炔烃（环辛炔）和叠氮化物之间的无铜点击化学反应是一种非常快速且稳定的反应。 它可用于荧光染料的快速标记。 该试剂是水溶性磺基-Cyanine5染料的衍生物，在红色通道中发射。 它可用于标记水性介质中的生物分子。

Absorption and emission spectra of sulfo-Cyanine5

sulfo-Cyanine5 DBCO

sulfo-Cyanine5 DBCO相关产品

TMRE, Tetramethylrhodamine ethyl ester, perchlorate

Orange-red fluorescent dye for staining active mitochondria and quantitate mitochondrial membrane potential in live cells.

橙红色荧光染料，用于染色活性线粒体和定量活细胞中的线粒体膜电位。

Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.

Cy5.5 NHS 是远红/近红外胺活性染料。

Cyanine5.5 amine

Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.

Cy5.5 的氨基衍生物，一种发射远红色荧光的花青染料。

General properties

Appearance: dark colored solid
Molecular weight: 981.27
Molecular formula: C53H57N4KO8S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

相关产品

  1. Mamot, A.; Sikorski, P.J.; Siekierska, A.; de Witte, P.; Kowalska, J.; Jemielity, J. Ethylenediamine derivatives efficiently react with oxidized RNA 3′ ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation. Nucleic Acids Research, 2022, 50(1), e3. doi: 10.1093/nar/gkab867
  2. Lim, S.; Kim, W.; Song, S.; Shim, M.K.; Yoon, H.Y.; Kim, B.-S.; Kwon, I.C.; Kim, K. Intracellular Uptake Mechanism of Bioorthogonally Conjugated Nanoparticles on Metabolically Engineered Mesenchymal Stem Cells. Bioconjugate Chemistry, 2021, 32(1), 199–214. doi: 10.1021/acs.bioconjchem.0c00640
  3. Desai, B.J.; Gonzalez, R.L. Multiplexed genomic encoding of non-canonical amino acids for labeling large complexes. Nature Chemical Biology, 2020, 16, 1129–1135. doi: 10.1038/s41589-020-0599-5

Sulfo-Cyanine5 tetrazine

Sulfo-Cyanine5 tetrazine,TCO（四嗪 – 反式环辛烯）连接是用于生物偶联的最快反应之一。 它是在四嗪和反式环辛烯或其他应变烯烃之间发生的逆电子需求[4+2] 环加成。

  • Products
  • Reactive dyes
  • Tetrazines
  • Sulfo-Cyanine5 tetrazine

Sulfo-Cyanine5 tetrazine

标签：sulfo-Cyanine5,sulfo-Cy5, Tetrazine, Copper free Click chemistry
货号 规格 价格 货期
133E0 1 mg 110.00$ in stock
233E0 5 mg 290.00$ in stock
333E0 10 mg 460.00$ in stock
433E0 25 mg 690.00$ in stock
533E0 50 mg 1270.00$ in stock
633E0 100 mg 1990.00$ in stock

TCO (tetrazine – trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefin.

Sulfo-Cyanine5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation based labeling.

This reagent possesses good aqueous solubility and stability in biological environments.

TCO（四嗪 – 反式环辛烯）连接是用于生物偶联的最快反应之一。 它是在四嗪和反式环辛烯或其他应变烯烃之间发生的逆电子需求[4+2] 环加成。

Sulfo-Cyanine5 四嗪是一种带有四嗪基团的荧光团衍生物，用于基于 TCO 连接的标记。

该试剂在生物环境中具有良好的水溶性和稳定性。

Absorption and emission spectra of Sulfo-Cyanine5

Sulfo-Cyanine5 tetrazine

Sulfo-Cyanine5 tetrazine相关产品

Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.

用于 Click Chemistry 的 Cy3 荧光染料的叠氮化物衍生物。

sulfo-Cyanine3 DBCO

Sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water soluble, general purpose fluorophore sulfo-Cyanine3 for Click Chemistry.

Sulfo-Cyanine3 DBCO 是用于 Click Chemistry 的水溶性通用荧光团 sulfo-Cyanine3 的环炔衍生物。

sulfo-Cyanine5.5 DBCO

Sulfo-Cyanine5.5 DBCO is a far-red, water-soluble reagent for copper-free reaction with azides.

Sulfo-Cyanine5.5 DBCO 是一种远红色的水溶性试剂，用于与叠氮化物的无铜反应。

General properties

Appearance: dark blue solid 深蓝色固体
Molecular weight: 864.09
Molecular formula: C42H46KN7O7S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine5 carboxylic acid,非活化磺基花青5羧酸，水溶性染料。 这种染料是高度亲水和水溶性的。 非磺化类似物可用。

  • Products
  • Reactive dyes
  • Carboxylic acids
  • Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine5 carboxylic acid

标签：sulfo-Cyanine5,sulfo-Cy5,Carboxylic acids
货号 规格 价格 货期
13390 1 mg 110.00$ in stock
23390 5 mg 210.00$ in stock
43390 25 mg 410.00$ in stock
53390 50 mg 695.00$ in stock
63390 100 mg 1190.00$ in stock

Non-activated sulfo-Cyanine5 carboxylic acid, water soluble dye. This dye is highly hydrophilic and water-soluble. Non-sulfonated analog is available.

The fluorophore is an equivalent of Cy5® carboxylic acid.

For labeling applications consider using pre-activated sulfo-Cy5 NHS ester

非活化磺基花青5羧酸，水溶性染料。 这种染料是高度亲水和水溶性的。 非磺化类似物可用。

荧光团相当于 Cy5® 羧酸。

对于标签应用，考虑使用预活化的磺基-Cy5 NHS 酯

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine5 carboxylic acid相关产品

AF488 NHS ester

AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.

AF488 NHS 酯，一种用于绿色通道的明亮、光稳定性和亲水性荧光染料。

Cyanine5 maleimide

Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.

Cy5 马来酰亚胺与硫醇反应生成染料标记的偶联物。

Cyanine7.5 carboxylic acid

Cyanine7.5 – unactivated NIR cyanine dye carboxylic acid.

Cy7.5 – 未活化的近红外花青染料羧酸。

General properties

Appearance: dark blue powder 深蓝色粉末
Molecular weight: 680,87
CAS number: 1144107-82-3 (potassium salt), 1121756-16-8 (inner salt), 2098639-31-5 (sodium salt)
Molecular formula: C32H37N2KO8S2
Solubility: Well soluble in water, DMF, DMSO (0.35 M = 240 g/L). Practically insoluble in non-polar organic solvents.
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

相关文献：

  1. Sun, X.; Du, J.D.; Hawley, A.; Tan, A.; Boyd, B.J. Magnetically-stimulated transformations in the nanostructure of PEGylated phytantriol-based nanoparticles for on-demand drug release. Colloids and Surfaces B: Biointerfaces, 2021, 207, 112005. doi: 10.1016/j.colsurfb.2021.112005
  2. Novoselova, M.V.; Abakumova, T.O.; Khlebtsov, B.N.; Zatsepin, T.S.; Lazareva, E.N.; Tuchin, V.V.; Zharov, V.P.; Gorin, D.A.; Galanzha, E.I. Optical clearing for photoacoustic lympho- and angiography beyond conventional depth limit in vivo. Photoacoustics, 2020, 20, 100186. doi: 10.1016/j.pacs.2020.100186
  3. Matoori, S.; Mooney, D.J. Near-Infrared Fluorescence Hydrogen Peroxide Assay for Versatile Metabolite Biosensing in Whole Blood. Small, 2020, 16(20), 2000369. doi: 10.1002/smll.202000369
  4. Pfister, J.; Summer, D.; Petrik, M.; Khoylou, M.; Lichius, A.; Kaeopookum, P.; Kochinke, L.; Orasch, T.; Haas, H.; Decristoforo, C. Hybrid Imaging of Aspergillus fumigatus Pulmonary Infection with Fluorescent, 68Ga-Labelled Siderophores. Biomolecules, 2020, 10, 168. doi: 10.3390/biom10020168
Cy® is a trademark of GE Healthcare.