NuGen Trio RNA-Seq 0506-32 0357-08 9143-96 30184297 0514-32

货号

品名

规格

0357-08

Trio RNA-Seq

8

0357-32

Trio RNA-Seq

32

0357-96

Trio RNA-Seq

96

0506-08

Trio RNA-Seq, Human rRNA AnyDeplete

8

0506-32

Trio RNA-Seq, Human rRNA AnyDeplete

32

0506-96

Trio RNA-Seq, Human rRNA AnyDeplete

96

0506-A01

Trio RNA-Seq, Human rRNA AnyDeplete

A01

0507-08

Trio RNA-Seq, Mouse rRNA AnyDeplete

8

0507-32

Trio RNA-Seq, Mouse rRNA AnyDeplete

32

0507-96

Trio RNA-Seq, Mouse rRNA AnyDeplete

96

0507-A01

Trio RNA-Seq, Mouse rRNA AnyDeplete

96

0514-32

Trio RNA-Seq ,custom AnyDeplete

32

0514-96

Trio RNA-Seq ,custom AnyDeplete

96

0514-A01

Trio RNA-Seq ,custom AnyDeplete

96

9143-96

Trio RNA-Seq + Dual Unique Index, Human rRNA AnyDeplete

96

9143-A01

Trio RNA-Seq + Dual Unique Index, Human rRNA AnyDeplete

A01

9150-96

Trio RNA-Seq + Dual Unique Index, Mouse rRNA AnyDeplete

96

30184297

Probe Respiratory Bact Trio AD w Human 32

32

30184298

Probe Respiratory Bact Trio AD w Human A01

A01

试剂盒特点:
1.样本起始量500pg-50ng total RNA起始;
2.Anydeplete技术去除rRNA,适用于人小鼠及定制去除任何想去除信息;
3.适用于微量样本,FFPE样本,拭子样本,外泌体样本等;
4.客户长用于做未知RNA病源及病毒检测。
5.单端有96个index及96个dual index,避免index hopping;
6.新推出探针可同时去除人rRNA及微生物rRNA。

试剂盒文献:
1.Chen, L., et. al. (2020). RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak. Emerging Microbes & Infections, doi: 10.1080/22221751.2020.1725399
2.Shen Z., et. al. (2020) Genomic diversity of SARS-CoV-2 in Coronavirus Disease 2019 patients. Clinical Infectious Diseases. doi:10.1093/cid/ciaa203
3.Gong Y., el. al. (2020) Sequence variation among SARS-CoV-2 isolates in Taiwan. bioRvix  doi: 10.1101/2020.03.29.014290
4.Maurano MT., et. al. (2020) Sequencing identifies multiple, early introductions of SARS-CoV2 to New York City Region. medRxiv doi: 10.1101/2020.04.15.20064931

NuGEN Technologies, Inc., based in CA, USA, a part of the Tecan Group, provides leading edge, the easiest, the fastest NGS library preparation kits for a broad range of sample types including DNA and RNA from whole tissues, FFPE samples, single cells and liquid biopsies in the market, based on their core technology:

– Single Primer Enrichment Technology (SPET) for DNA: Directly enrich regions of DNA for targeted re-sequencing, SNP (SNV) detection, CNV analysis, viral detection, and more.

– Single Primer Enrichment Technology (SPET) for RNA: Directly enrich cDNA targets for gene fusion detection and discovery.

– Single Primer Isothermal Amplification (SPIA): Robust cDNA amplification for multiple workflows.

– AnyDeplete: Stranded RNA-Seq library preparation with targeted transcript depletion.

ZYMO RESEARCH Pico Methyl-Seq Library Prep Kit微量甲基化建库试剂盒

货号 品名 规格

D5455

Pico Methyl-Seq Library Prep Kit (10 preps.) –

10

D5456

Pico Methyl-Seq Library Prep Kit (25 preps.)

25

产品描述:

Pico Methyl-Seq™ Library Prep Kit为WGBS建库提供了一个简化建库流程,投入的DNA在*初的甲基化转化步骤被随机打断,后面有三部特异引物扩增步骤。可以做到10pg DNA起始(包括FFPE样本),这个能够完美的应用于珍贵的,样本量有限的及靶向富集的样本甲基化分析。

特点:

1.    样本起始量:10pg-100ng DNA;

2.    测序平台:illumina测序平台;

3.    操作时间:6h

Celero™ DNA-Seq DNA 建库试剂盒

Preparation Kit

货号

品名

规格

0360-24

Celero DNA-Seq

24

0360A-A01

Celero DNA-Seq Barcode Set A

A01

0360B-A01

Celero DNA-Seq Barcode Set B

96

0360A-UDI

Celero DNA-Seq, UDI

96

0360B-UDI

Celero DNA-Seq, UDI

96

产品描述:

1.      DNA起始量10ng-500ng200ng-1ug可进行PCR free建库;

2.      没有GC偏好性,无接头二聚体;

3.      具有384dual index384个单端index

4.      除接头外只有3管试剂(混匀mix),操作简单;

 

试剂盒文献:

1.      A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds

1 January, 2019

Khanday I, Skinner D, Yang B, Mercier R, Sundaresan V

The molecular pathways that trigger the initiation of embryogenesis after fertilization in flowering plants, and prevent its occurrence without fertilization, are not well understood1. Here we show in rice (Oryza sativa) that BABY BOOM1 (BBM1), a member of the AP2 family2 of transcription factors that is expressed in sperm cells, has a key role in this process. Ectopic expression of BBM1 in the egg cell is sufficient for parthenogenesis, which indicates that a single wild-type gene can bypass the fertilization checkpoint in the female gamete. Zygotic expression of BBM1 is initially specific to the male allele but is subsequently biparental, and this is consistent with its observed auto-activation. Triple knockout of the genes BBM1, BBM2 and BBM3 causes embryo arrest and abortion, which are fully rescued by male-transmitted BBM1. These findings suggest that the requirement for fertilization in embryogenesis is mediated by male-genome transmission of pluripotency factors. When genome editing to substitute mitosis for meiosis (MiMe)3,4 is combined with the expression of BBM1 in the egg cell, clonal progeny can be obtained that retain genome-wide parental heterozygosity. The synthetic asexual-propagation trait is heritable through multiple generations of clones. Hybrid crops provide increased yields that cannot be maintained by their progeny owing to genetic segregation. This work establishes the feasibility of asexual reproduction in crops, and could enable the maintenance of hybrids clonally through seed propagation5,6.

Pages: 91-95   doi: 10.1038/s41586-018-0785-8

2.      Improved genomic resources for the black tiger prawn (Penaeus monodon)

4 February, 2020

Van Quyen D, Gan HM, Lee YP, Nguyen DD, Nguyen TH, Tran XT, Nguyen VS, Khang DD, Austin CM

World production of farmed crustaceans was 7.8 million tons in 2016. While only making up approximately 10% of world aquaculture production, crustaceans are generally high-value species and can earn significant export income for producing countries. Viet Nam is a major seafood producing country earning USD 7.3 billion in 2016 in export income with shrimp as a major commodity. However, there is a general lack of genomic resources available for shrimp species, which is challenging to obtain due to the need to deal with large repetitive genomes, which characterize many decapod crustaceans. The first tiger prawn (P. monodon) genome assembly was assembled in 2016 using the standard Illumina PCR-based pair-end reads and a computationally-efficient but relatively suboptimal assembler, SOAPdenovo v2. As a result, the current P. monodon draft genome is highly fragmented (> 2 million scaffolds with N50 length of &lt;1000 bp), exhibiting only moderate genome completeness (< 35% BUSCO complete single-copy genes). We sought to improve upon the recently published P. monodon genome assembly and completeness by generating Illumina PCR-free pair-end sequencing reads to eliminate genomic gaps associated with PCR-bias and performing de novo assembly using the updated MaSurCA de novo assembler. Furthermore, we scaffolded the assembly with low coverage Nanopore long reads and several recently published deep Illumina transcriptome paired-end sequencing data, producing a final genome assembly of 1.6 Gbp (1,211,364 scaffolds; N50 length of 1982 bp) with an Arthropod BUSCO completeness of 96.8%. Compared to the previously published P. monodon genome assembly from China (NCBI Accession Code: NIUS01), this represents an almost 20% increase in the overall BUSCO genome completeness that now consists of more than 90% of Arthropod BUSCO single-copy genes. The revised P. monodon genome assembly (NCBI Accession Code: VIGR01) will be a valuable resource to support ongoing functional genomics and molecular-based breeding studies in Vietnam.

Pages: 100751   doi: 10.1016/j.margen.2020.100751

3.      Sequence variation among SARS-CoV-2 isolates in Taiwan

1 January, 2020

Gong YN, Tsao KC, Hsiao MJ, Huang CG, Huang PN

Taiwan experienced two waves of imported cases of coronavirus disease 2019 (COVID-19), first from China in January to late February, followed by those from other countries starting in early March. Additionally, several cases could not be traced to any imported cases and were suspected as sporadic local transmission. Twelve full viral genomes were determined in this study by Illumina sequencing either from virus isolates or directly from specimens, among which 5 originated from clustered infections. Phylogenetic tree analysis revealed that these sequences were in different clades, indicating that no major strain has been circulating in Taiwan. A deletion in open reading frame 8 was found in one isolate. Only a 4-nucleotide difference was observed among the 5 genomes from clustered infections.

  doi: 10.1101/2020.03.29.014290v1.abstract

4.      Whole genome assembly of the snout otter clam, Lutraria rhynchaena, using Nanopore and Illumina data, benchmarked against bivalve genome assemblies

22 October, 2019

Thai BT, Lee YP, Gan HM, Austin CM, Croft LJ

Production of cultured bivalve molluscs was 17.1 million tons in 2016 accounting for 21.4% of global aquaculture production (FAO, 2018). The lack of genomic resources coupled with limited understanding of the molecular basis of gene expression and phenotypic variation have limited advances in aquaculture-based productivity of marine bivalves. Understanding the molecular basis of phenotypic variation and gene function is therefore important for selective breeding programs for traits such as increased growth and disease resistance. Similarly, whole genome assembles support GWAS studies to identify trait-specific loci and for genomic-based selective breeding. To this end, whole-genome sequencing has been conducted on several commercial bivalve species, including the edible oysters Crassostrea virginica (Gómez-Chiarri et al., 2015), Crassostrea gigas (Gerdol et al., 2015), pearl oysters (Takeuchi et al., 2012) and clams (Mun et al., 2017). However, in general, genomic data for bivalve molluscs, which includes a taxonomically diverse group of species, is sparse (Takeuchi et al., 2012; Zhang et al., 2012; Murgarella et al., 2016; Du et al., 2017; Li et al., 2017; Mun et al., 2017; Sun et al., 2017; Uliano-Silva et al., 2017; Wang et al., 2017; Li et al., 2018; Powell et al., 2018; Renaut et al., 2018; Bai et al., 2019). In this study we present the first genomic resources for a species of clam from the superfamily Mactroidea and for a Vietnamese shellfish species and generate a draft reference genome to form the basis of on-going selective breeding studies. This study also demonstrates the efficacy of using Oxford Nanopore Technology (ONT) reads to scaffold a bivalve genome assembly and shows the value of these relatively inexpensive long reads for spanning large repetitive regions and overcoming complex assembly issues caused by high heterozygosity, which typically confounds short read only assemblies. The quality of our assembly is also benchmarked against other bivalves genome assemblies and we present an initial phylogenomic analysis for the class Bivalvia, which illustrates the value and potential of the increasing number of high quality genomic data sets for phylogenetics.

  doi: 10.3389/fgene.2019.01158

Zymo-Seq RiboFree Total RNA Library Kit

货号

品名

规格

R3000

Zymo-Seq RiboFree Total RNA Library Kit

12次

R3001

Zymo-Seq RiboFree Universal cDNA Kit

12次

R3003

Zymo-Seq RiboFree Total RNA Library Kit

96次

试剂盒特点:
1.样本起始量100ng-5ug total RNA;
2.5h完成rRNA去除及文库构建;
3.适用于所有物种rRNA去除,无需使用探针;
4.96个dual index,可以避免index hopping;
5.产物包含mRNA及LncRNA;