标签： ROX (Rhodamine X

ROX reference dye for qPCR

ROX reference dye for qPCR,这种 ROX 配方是用于 qPCR 应用的被动参考染料。 推荐使用这种染料来标准化 qPCR 报告染料的荧光强度，例如 dsGreen 或 TaqMan 探针。 它还允许纠正移液错误和仪器漂移，例如灯强度输出随时间的变化。

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  • ROX reference dye for qPCR

ROX reference dye for qPCR

标签：ROX (Rhodamine X, Rhodamine 101), PCR and qPCR
货号 规格 价格 货期
31110 500 uL 22.00$ in stock
41110 1 mL 30.00$ in stock
51110 25 mL 230.00$ in stock
61110 50mL 300.00$ in stock

This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as dsGreen or TaqMan probes. It also allows to correct for pipeting errors, and instrumental drift such as change of lamp intensity output over time.

The dye contains 25 μM solution of 5-carboxy-ROX (rhodamine-X) reference dye in 10mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.

ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

这种 ROX 配方是用于 qPCR 应用的被动参考染料。 推荐使用这种染料来标准化 qPCR 报告染料的荧光强度，例如 dsGreen 或 TaqMan 探针。 它还允许纠正移液错误和仪器漂移，例如灯强度输出随时间的变化。

该染料含有 25 μM 5-羧基-ROX (rhodamine-X) 参比染料在 10mM Tris-HCl (pH 8.6)、0.1mM EDTA 和 0.01% Tween®-20 中的溶液。

ROX 参考染料与所有带有 ROX 通道的 qPCR 仪器兼容。 大多数仪器需要 500 nM 或 50 nM 的 ROX 末端浓度。

ROX reference dye for qPCR相关产品

Cyanine3 amine

Cyanine3 is an fluorophore with free amino group (amino-dye). It can be conjugated with NHS esters, carboxy groups (after activation), and epoxides.

Cy3 是一种带有游离氨基的荧光团（氨基染料）。 它可以与 NHS 酯、羧基（活化后）和环氧化物结合。

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cy7 carboxylic acid is a non-reactive fluorescent dye which can be used as a NIR (near infrared) marker.

Sulfo-Cy7 羧酸是一种非反应性荧光染料，可用作 NIR（近红外）标记。

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 free unactivated monofunctional carboxylic acid, water-soluble dye.

Sulfo-Cy3 不含未活化的单官能羧酸，水溶性染料。

General properties

Appearance: pink solution
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
Emission maximum, nm: 591

相关文献：

  1. Zhuo, X.-Z.; Bai, K.; Wang, Y.; Liu, P.; Xi, W.; She, J.; Liu, J. Long-chain non-coding RNA-GAS5 / hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2. Bioscience Reports, 2021, 41(9), BSR20202232. doi: 10.1042/BSR20202232
  2. Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038

ROX DBCO, 5-isomer

ROX DBCO, 5-isomer,ROX DBCO 是明亮的罗丹明染料罗丹明 X 和 DBCO（二苯并环辛炔）的共轭物，DBCO（二苯并环辛炔）是一种反应性基团，包含用于与叠氮化物共轭的应变炔键。

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  • ROX DBCO, 5-isomer

ROX DBCO, 5-isomer

标签：ROX (Rhodamine X, Rhodamine 101), Copper free Click chemistry, Cycloalkyne
112F0 1 mg 110.00$ in stock
212F0 5 mg 290.00$ in stock
412F0 25 mg 690.00$ in stock
512F0 50 mg 1270.00$ in stock
612F0 100 mg 1990.00$ in stock

ROX DBCO is a conjugate of bright rhodamine dye rhodamine X, and DBCO (dibenzocyclooctyne) – a reactive group that contains a strained alkyne bond for the conjugation with azides.

This reagent is useful for the labeling of various azides with ROX dye.

This derivative contains pure 5-isomer of ROX.

ROX DBCO 是明亮的罗丹明染料罗丹明 X 和 DBCO（二苯并环辛炔）的共轭物，DBCO（二苯并环辛炔）是一种反应性基团，包含用于与叠氮化物共轭的应变炔键。

该试剂可用于用 ROX 染料标记各种叠氮化物。

该衍生物含有纯的 ROX 5-异构体。

Absorption and emission spectra of ROX

ROX DBCO, 5-isomer

ROX DBCO, 5-isomer相关产品

Sulfo-Cyanine7 amine

Water-soluble NIR dye amine, sulfonated derivative of Cyanine7 dye, for the modification via enzymatical transamination.

水溶性 NIR 染料胺，Cyanine7 染料的磺化衍生物，用于通过酶促氨基转移进行修饰。

ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

ROX（又名罗丹明 X 或罗丹明 101）叠氮化物，用于通过 Click Chemistry 进行生物分子标记。

BDP 630/650 tetrazine

BDP 630/650 is a borondipyrromethene dye for Cyanine5 channel. This is a tetrazine derivative for the coupling with strained cycloalkenes.

BDP 630/650 是用于 Cyanine5 通道的硼二吡咯亚甲基染料。 这是一种用于与应变环烯烃偶联的四嗪衍生物。

General properties

Appearance: red solid 红色固体
Mass spec M+ increment: 834.4
Molecular weight: 835.00
Molecular formula: C54H50N4O5
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00

ROX maleimide, 5-isomer

ROX maleimide, 5-isomer,ROX 是一种明亮的罗丹明染料，最大发射波长约为 580 nm。 它广泛用于标记生物分子。 与其他罗丹明染料一样，它有两种异构体，5-ROX 和 6-ROX。 尽管这些异构体具有几乎相同的光谱特性，但需要将它们分离以避免在 HPLC 或电泳中标记产物加倍。

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ROX maleimide, 5-isomer

标签：ROX (Rhodamine X, Rhodamine 101), Maleimides
货号 规格 价格 货期
11280 1 mg 110.00$ in stock
21280 5 mg 210.00$ in stock
41280 25 mg 410.00$ in stock
51280 50 mg 695.00$ in stock
61280 100 mg 1190.00$ in stock

ROX is a bright rhodamine dye with emission maximum around 580 nm. It is widely used for the labeling of biomolecules. As other rhodamine dyes, it comes as two isomers, 5-ROX and 6-ROX. Although these isomers have almost identical spectral properties, they need to be separated to avoid doubling of labeled products on HPLC or electrophoresis.

This derivative contains thiol-reactive maleimide moiety for the attachment of ROX fluorophore to SH groups, such as cystein residues in proteins and peptides.

ROX 是一种明亮的罗丹明染料，最大发射波长约为 580 nm。 它广泛用于标记生物分子。 与其他罗丹明染料一样，它有两种异构体，5-ROX 和 6-ROX。 尽管这些异构体具有几乎相同的光谱特性，但需要将它们分离以避免在 HPLC 或电泳中标记产物加倍。

该衍生物含有硫醇反应性马来酰亚胺部分，用于将 ROX 荧光团连接到 SH 基团，例如蛋白质和肽中的半胱氨酸残基。

Absorption and emission spectra of ROX

ROX maleimide, 5-isomer

ROX maleimide, 5-isomer相关文献

BDP R6G maleimide

BDP R6G is a borondipyrromethene dye tuned to R6G (rhodamine 6G) channel. This is a thiol reactive maleimide for the labeling of protein cysteine residues, and other thiols.

BDP R6G 是一种调谐到 R6G（罗丹明 6G）通道的硼二吡咯亚甲基染料。 这是一种硫醇反应性马来酰亚胺，用于标记蛋白质半胱氨酸残基和其他硫醇。

Sulfo-Cyanine7 maleimide

Water soluble near infrared dye maleimide for the labeling of proteins and peptides.

水溶性近红外染料马来酰亚胺，用于标记蛋白质和肽。

FAM maleimide, 6-isomer

Fluorescein (FAM) maleimide is a thiol reactive dye derived from isomerically pure 6-FAM. Fluorescein is a bright dye that is compatible with various fluorescence measurement instruments.

荧光素 (FAM) 马来酰亚胺是一种衍生自异构纯 6-FAM 的硫醇活性染料。 荧光素是一种与各种荧光测量仪器兼容的明亮染料。

General properties

Appearance: red powder  红色粉末
Mass spec M+ increment: 656.3
Molecular weight: 656.73
Molecular formula: C39H36N4O6
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00

ROX alkyne, 5-isomer

ROX alkyne, 5-isomer,随着其量子产率接近统一，ROX 已成为 qPCR 和显微镜检查的流行染料。 这种明亮的荧光团可用作铜催化点击化学反应的炔烃。这是 ROX 的纯 5 异构体。

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ROX alkyne, 5-isomer

标签：ROX (Rhodamine X, Rhodamine 101), Alkynes
货号 规格 价格 货期
A12B0 1 mg 110.00$ in stock
B12B0 5 mg 210.00$ in stock
C12B0 10 mg 310.00$ in stock
D12B0 25 mg 410.00$ in stock
E12B0 50 mg 695.00$ in stock
F12B0 100 mg 1190.00$ in stock

With its quantum yield approaching unity, ROX has become a popular dye for qPCR and microscopy. This bright fluorophore is available as an alkyne for copper-catalyzed Click chemistry reaction.

This is a pure 5-isomer of ROX.

ROX is prone to oxidation. Therefore, its derivatives should be preferably stored in inert atmosphere.

随着其量子产率接近统一，ROX 已成为 qPCR 和显微镜检查的流行染料。 这种明亮的荧光团可用作铜催化点击化学反应的炔烃。

这是 ROX 的纯 5 异构体。

ROX容易氧化。 因此，其衍生物应优选储存在惰性气氛中。

Absorption and emission spectra of ROX

ROX alkyne, 5-isomer

ROX alkyne, 5-isomer相关产品

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.

胺反应性 Cyanine3 染料 NHS 酯。

Sulfo-Cyanine3 alkyne

A water soluble dye alkyne for copper-catalyzed Click Chemistry. The fluorophore Sulfo-Cyanine3 is a bright and photostable dye for the Cy3® channel.

一种用于铜催化点击化学的水溶性染料炔烃。 荧光团 Sulfo-Cyanine3 是一种用于 Cy3® 通道的明亮且光稳定的染料。

R6G alkyne, 6-isomer

R6G (rhodamine 6G) terminal alkyne for copper catalyzed Click chemistry reaction with azides. Pure 6-isomer of the dye.

R6G（罗丹明 6G）末端炔烃用于铜催化与叠氮化物的点击化学反应。 染料的纯 6-异构体。

General properties

Appearance: purple solid 紫色固体
Mass spec M+ increment: 571.3
Molecular weight: 571.66
CAS number: 2264016-88-6
Molecular formula: C36H33N3O4
Solubility: good in DMSO, DMF, methanol, ethanol
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.00