Microbiologics EZ-Accu Shot™ Starved Cells定量菌株 适合R2A平板 0524SC等

品牌:Microbiologics,简称MBL 
产品系列:EZ-Accu Shot™ Starved Cells
规格:5支/盒
菌株代数:小于等于ATCC3代
产地:美国

EZ-Accu Shot™ Starved Cells

日本药典JP里面提到需要对R2A琼脂进行培养基促生长实验,JP里面说在进行实验之前需要用“挨饿”3天的菌株进行挑战实验。
为了简化和加快R2A琼脂的促生长实验,我们发明了EZ-Accu Shot™ Starved Cells系列产品。
这种被测试过的菌株已经在冻干之前进行了“挨饿”,每取1ml涂布于R2A琼脂上后,可以符合在R2A琼脂上恢复生长为50-200CUF/个平板。

关于“挨饿”的个人理解:由于R2A琼脂平板是用于检测纯化水中的细菌含量,而纯化水中含有的细菌一般是需要极少营养物质就可以存活的,所以需要让菌株在无营养环境下待3天,也就是“挨饿3天”,模拟纯化水中的寡样细菌,以便达到最佳的R2A促生长实验效果。

Laboratories following Japanese Pharmacopeia (JP) guidelines know the challenges of performing Media Growth Promotion Testing on R2A agar. The JP states that R2A media should be challenged with strains that have been starved for 3 days prior to conducting the test. To simplify and accelerate test procedures for growth promotion of R2A agar, we have developed EZ-Accu Shot™ Starved Cells. These test-ready control strains have been starved prior to lyophilization and deliver the required concentration of 50-200 CFU per inoculum on R2A media.

产品包装:
一盒内有5支菌株,每支有1个冻干菌株颗粒

每个冻干颗粒的菌含量为500-2000CFU之间,如果用1ml无菌水溶解,则为50-200CFU/0.1ml;如果用10ml无菌水溶解,则为50-200CFU/1ml

产品列表:

货号 产品描述 备注
01110SC Methylobacterium extorquens derived from ATCC® BAA-2500™*
扭脱甲基杆菌ATCC BAA-2500
Quantitative microorganism preparation that delivers 50-200 CFU per 1 ml of inoculumn on R2A media.
Designed for laboratories performing the Growth Promotion Test of R2A media according to the Japanese Pharmacopeia (JP).
No dilutions required. Kit contains five vials of a single, quantitative microorganism (1 lyophilized pellet per vial).
0524SC Pseudomonas protegens (G) derived from ATCC® 17386™*
防御假单胞菌ATCC17386
Quantitative microorganism preparation that delivers 50-200 CFU per 1 ml of inoculumn on R2A media.
 Designed for laboratories performing the Growth Promotion Test of R2A media according to the Japanese Pharmacopeia (JP).
No dilutions required. Kit contains five vials of a single, quantitative microorganism (1 lyophilized pellet per vial).