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- Reactive dyes
- Dye NHS esters
- R6G NHS ester, 5-isomer
R6G NHS ester, 5-isomer
| Cat. # | Quantity | Price | Lead time | |
|---|---|---|---|---|
| 14220 | 1 mg | 110.00$ | 8 days | |
| 24220 | 5 mg | 210.00$ | 5 days | |
| 44220 | 25 mg | 410.00$ | 8 days | |
| 54220 | 50 mg | 695.00$ | 5 days | |
| 64220 | 100 mg | 1190.00$ | 5 days |
Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.
This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.
罗丹明 6G (R6G) 是罗丹明系列的呫吨染料，长期以来一直用于标记寡核苷酸和 DNA。 就像其他呫吨一样，R6G 有两种异构体，5 和 6 异构体。 尽管它们的吸收和发射光谱几乎无法区分，但需要分离异构体以避免在纯化过程中分离标记分子，即在电泳上出现双 HPLC 峰和双点。
这是一种胺反应性 NHS 酯，衍生自 R6G 的纯 5-异构体。 罗丹明 6G 非常亮（即使用作量子产率标准，产率 0.95）。 该染料可溶于有机溶剂，例如 DMF 或 DMSO。 它们可用作标记反应的助溶剂。
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Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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Pure isomer 6-ROX NHS ester for amine labeling.
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| Appearance: | dark colored solid |
| Molecular weight: | 555.58 |
| Molecular formula: | C31H29N3O7 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 519 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 546 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
