标签： R6G

lumiprobe -R6G NHS ester, 5-isomer/14220/24220

lumiprobe -R6G NHS ester, 5-isomer/14220/24220,lumiprobe -R6G NHS ester, 5-isomer/14220/24220,这是一种胺反应性 NHS 酯，衍生自 R6G 的纯 5-异构体。 罗丹明 6G 非常亮（即使用作量子产率标准，产率 0.95）。 该染料可溶于有机溶剂，例如 DMF 或 DMSO。 它们可用作标记反应的助溶剂。

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  • R6G NHS ester, 5-isomer

R6G NHS ester, 5-isomer

标签：NHS esters, R6G
Cat. # Quantity Price Lead time
14220 1 mg 110.00$ 8 days

24220 5 mg 210.00$ 5 days

44220 25 mg 410.00$ 8 days

54220 50 mg 695.00$ 5 days

64220 100 mg 1190.00$ 5 days

Rhodamine 6G (R6G) is a xanthene dye of rhodamine series that has been used for the labeling of oligonucleotides and DNA for quite a long time. Just like other xanthenes, R6G comes as two isomers, 5- and 6-isomer. Even though their absorption and emission spectra are virtually indistinguishable, the isomers need to be separated to avoid separation of the labeled molecules during their purification, i.e. double HPLC peaks and double spots on electrophoresis.

This is an amine reactive NHS ester derived from a pure 5-isomer of R6G. Rhodamine 6G is very bright (even used as a quantum yield standard, yield 0.95). The dye is soluble in organic solvents, such as DMF, or DMSO. They can be used as co-solvents for the labeling reactions.

罗丹明 6G (R6G) 是罗丹明系列的呫吨染料，长期以来一直用于标记寡核苷酸和 DNA。 就像其他呫吨一样，R6G 有两种异构体，5 和 6 异构体。 尽管它们的吸收和发射光谱几乎无法区分，但需要分离异构体以避免在纯化过程中分离标记分子，即在电泳上出现双 HPLC 峰和双点。

这是一种胺反应性 NHS 酯，衍生自 R6G 的纯 5-异构体。 罗丹明 6G 非常亮（即使用作量子产率标准，产率 0.95）。 该染料可溶于有机溶剂，例如 DMF 或 DMSO。 它们可用作标记反应的助溶剂。

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TAMRA NHS ester, 5-isomer

Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.

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ROX NHS ester, 5-isomer

Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.

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ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

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General properties

Appearance: dark colored solid
Molecular weight: 555.58
Molecular formula: C31H29N3O7
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

R6G NHS ester, 5-isomer

R6G phosphoramidite, 6-isomer

R6G phosphoramidite, 6-isomer,用于寡核苷酸合成的 R6G 亚磷酰胺，纯 6 异构体。

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  • R6G phosphoramidite, 6-isomer

R6G phosphoramidite, 6-isomer

标签：Oligonucleotide synthesis,Phosphoramidite,R6G
货号 规格 价格 货期
C5260 100 mg – in stock
D5260 250 mg 190.00$ in stock
F5260 1 g 590.00$ in stock
G5260 5 g 2390.00$ in stock
H5260 10 g please inquire 10 days

R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.

R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.

R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.

用于寡核苷酸合成的 R6G 亚磷酰胺，纯 6 异构体。

R6G（罗丹明 6G）是罗丹明家族的一种呫吨染料，具有高荧光量子产率和高摩尔消光系数。 相对于荧光素，6-R6G 的最大吸收 (518 nm) 和发射 (542 nm) 转移到长波区域。

R6G 可用作 DNA 测序和短串联重复 (STR) 分析中的 FRET 受体。 该染料通常用于生产用于定量 PCR 的 TaqMan 探针。 与 HEX 和 JOE 染料相比，R6G 与 BHQ1 猝灭剂组合在分子信标探针中具有更强的猝灭作用，显着降低了 PCR 中的背景荧光。

Usage:

Coupling: 10 minutes.

Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.

Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.

耦合：10 分钟。

脱保护：叔丁胺水溶液 (1:3 v/v) 在 55°С 下过夜。

由于 R6G 完全且不可逆地降解，因此不得使用氨水和 AMA（30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v）将修饰的寡核苷酸从固相载体上脱保护。

R6G phosphoramidite, 6-isomer R6G phosphoramidite, 6-isomer R6G phosphoramidite, 6-isomer

Absorption and emission spectra of R6G, 6-isomer

R6G phosphoramidite, 6-isomer

R6G phosphoramidite, 6-isomer相关产品

ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

ROX（又名罗丹明 X 或罗丹明 101）叠氮化物，用于通过 Click Chemistry 进行生物分子标记。

Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.

用于 Click Chemistry 的 Cyanine3 荧光染料的叠氮化物衍生物。

ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

用于胺标记的纯异构体 6-ROX NHS 酯。

General properties

Appearance: Yellowish foam
Molecular weight: 949.93
CAS number: 1355330-47-0
Molecular formula: C46H54F6N5O8P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下最多保存 2 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 518
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 542
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile

R6G alkyne, 6-isomer

R6G alkyne, 6-isomer,罗丹明6G（R6G）是罗丹明系列的呫吨染料。 与荧光素相比，它的发射变为红色。 它也更耐光。 荧光团具有高量子产率。该 R6G 产品包含一个末端炔烃部分，用于在铜 (I) 催化剂存在下与叠氮化物结合。

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  • R6G alkyne, 6-isomer

R6G alkyne, 6-isomer

标签：Alkynes, R6G
货号 规格 价格 货期
A52B0 1 mg 110.00$ in stock
B52B0 5 mg 210.00$ in stock
C52B0 10 mg 310.00$ in stock
D52B0 25 mg 410.00$ in stock
E52B0 50 mg 695.00$ in stock
F52B0 100 mg 1190.00$ in stock

Rhodamine 6G (R6G) is a xanthene dye of rhodamine series. Compared to fluorescein, its emission is shifted to red. It is also much more photostable. The fluorophore has a high quantum yield.

This R6G product contains a terminal alkyne moiety for the conjugation with azides in the presence of copper (I) catalyst.

罗丹明6G（R6G）是罗丹明系列的呫吨染料。 与荧光素相比，它的发射变为红色。 它也更耐光。 荧光团具有高量子产率。

该 R6G 产品包含一个末端炔烃部分，用于在铜 (I) 催化剂存在下与叠氮化物结合。

Absorption and emission spectra of 6-R6G

R6G alkyne, 6-isomer

R6G alkyne, 6-isomer相关产品

BDP FL alkyne

Borondipyrromethene dye alkyne for Click Chemistry. Bright and photostable dye for FAM channel.

用于 Click Chemistry 的硼二吡咯亚甲基染料炔烃。 用于 FAM 通道的明亮和光稳定染料。

ROX alkyne, 5-isomer

Rhodamine X (ROX, Rhodamine 101) alkyne derivative for copper catalyzed Click chemistry. ROX is a bright red-emitting dye.

用于铜催化点击化学的罗丹明 X（ROX，罗丹明 101）炔烃衍生物。 ROX 是一种明亮的红色发射染料。

FAM alkyne, 6-isomer

Fluorescein dye alkyne for Click Chemistry, pure 6-isomer.

用于 Click Chemistry 的荧光素染料炔烃，纯 6 异构体。

General properties

Appearance: dark colored solid 深色固体
Mass spec M+ increment: 494.2
Molecular weight: 495.57
Molecular formula: C30H29N3O4
Solubility: good in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 518
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 542
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

相关文献：

  1. Guarin, M.; Faelens, R.; Giusti, A.; De Croze, N.; Léonard, M.; Cabooter, D.; Annaert, P.; de Witte, P.; Ny, A. Spatiotemporal Imaging and Pharmacokinetic of Fluorescent Compounds in Zebrafish Eleuthero-Embryos After Different Routes of Administration. Research Square, preprint. doi: 10.21203/rs.3.rs-237483/v1
  2. Gao, F.; Hunter, A.; Qu, S.; Hoffman, J.R.; Gao, P.; Phillip, W.A. Interfacial Junctions Control Electrolyte Transport Through Charge-Patterned Membranes. ACS Nano, 2019, 13(7), 7655–7664. doi: 10.1021/acsnano.9b00780

R6G azide, 5-isomer

R6G azide, 5-isomer,R6G（罗丹明 6G）是一种发黄光的呫吨染料。 它以前已用于 DNA 测序。 现在这种染料常用于 qPCR。 这是 R6G 的纯 5-异构体，带有叠氮基。

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  • R6G azide, 5-isomer

R6G azide, 5-isomer

标签：Azides, R6G,5-isomer
货号 规格 价格 货期
A4430 1 mg 110.00$ in stock
B4430 5 mg 210.00$ in stock
C4430 10 mg 310.00$ in stock
D4430 25 mg 410.00$ in stock
E4430 50 mg 695.00$ in stock

R6G (rhodamine 6G) is a yellow emitting xanthene dye. It has been used for DNA sequencing before. Now this dye is often used for qPCR. This is a pure 5-isomer of R6G with azide group.

R6G（罗丹明 6G）是一种发黄光的呫吨染料。 它以前已用于 DNA 测序。 现在这种染料常用于 qPCR。 这是 R6G 的纯 5-异构体，带有叠氮基。

Absorption and emission spectra for 5-R6G

R6G azide, 5-isomer

R6G azide, 5-isomer相关产品

Cyanine3.5 azide

Cyanine3.5 azide for biomolecule labeling through Click Chemistry.

通过 Click Chemistry 用于生物分子标记的 Cy3.5 叠氮化物。

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

胺反应性 Cyanine5 活化酯，用于标记蛋白质、肽和其他分子。

Cyanine5.5 azide

Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.

用于 Click Chemistry 的远红/近红外荧光 Cy5.5 染料衍生物。

General properties

Appearance: orange to brown powder 橙色至棕色粉末
Mass spec M+ increment: 540.3
Molecular weight: 540.61
Molecular formula: C30H32N6O4
Solubility: good in DMF, DMSO, alcohols
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 546
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17