标签： Protein labeling

BDP R6G NHS ester

BDP R6G NHS ester，BDP R6G NHS 酯是一种胺反应性硼二吡咯亚甲基染料（BODIPY® R6G NHS 酯的类似物），其吸收和发射光谱与 R6G 相似。

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  • BDP R6G NHS ester

BDP R6G NHS ester

NHS esters BDP R6G Protein labeling
货号 规格 价格 货期
14420 1 mg 110.00$ in stock
24420 5 mg 210.00$ in stock
44420 25 mg 410.00$ in stock
54420 50 mg 695.00$ in stock
64420 100 mg 1190.00$ in stock

BDP R6G NHS ester is an amine-reactive borondipyrromethene dye (an analog of BODIPY® R6G NHS ester) that has absorption and emission spectra similar to R6G.

BDP R6G is a bright and photostable dye that exhibits long living fluorescence that has little pH dependence. Due to the long fluorescence lifetime, this dye is useful for fluorescence polarization assays, and for two-photon experiments.

The NHS ester function can be conjugated with various amine groups, including those present in proteins and peptides.

BDP R6G NHS 酯是一种胺反应性硼二吡咯亚甲基染料（BODIPY® R6G NHS 酯的类似物），其吸收和发射光谱与 R6G 相似。

BDP R6G 是一种明亮且光稳定的染料，具有长寿命荧光，几乎不依赖 pH 值。 由于荧光寿命长，这种染料可用于荧光偏振测定和双光子实验。

NHS 酯官能团可以与各种胺基结合，包括存在于蛋白质和肽中的胺基。

BDP R6G NHS ester BDP R6G NHS ester BDP R6G NHS ester BDP R6G NHS ester

Absorption and emission spectra of BDP R6G fluorophore

BDP R6G NHS ester

BDP R6G NHS ester相关产品

BDP R6G carboxylic acid

Free carboxylic acid derivative of BDP R6G – a borondipyrromethene dye with absorption and emission spectra resembling R6G rhodamine.

BDP R6G 的游离羧酸衍生物 – 一种具有类似于 R6G 罗丹明的吸收和发射光谱的硼二吡咯亚甲基染料。

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.

胺反应性 Cyanine3 染料 NHS 酯。

BDP TR NHS ester

BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.

BDP TR 是一种硼二吡咯亚甲基染料。 具有高量子产率的明亮且光稳定的荧光团。

General properties

Appearance: dark-green crystals
Mass spec M+ increment: 322.1
Molecular weight: 437.21
CAS number: 335193-70-9, 1443457-59-7
Molecular formula: C22H18BF2N3O4
IUPAC name: 2,5-Dioxo-1-pyrrolidinyl 3-(4,4-difluoro-5-phenyl-3a,4a-diaza-4-bora-s-indacen-3-yl)propionate
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 530
ε, L⋅mol−1⋅cm−1: 76000
Emission maximum, nm: 548
Fluorescence quantum yield: 0.96
CF260: 0.17
CF280: 0.18

  1. Lee, S.A.; Biteen, J.S. Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles. Journal of Physical Chemistry Letters, 2019, 10, 5764–5769. doi: 10.1021/acs.jpclett.9b02480
BODIPY® is a trademark of Life Technologies.

BDP 630/650 X NHS ester

BDP 630/650 X NHS ester,BDP 630/650 是一种硼二吡咯亚甲基荧光团，具有高摩尔消光系数、出色的量子产率和相对较长的激发态寿命。 因此，这种荧光团可用于荧光偏振测定，以检测分子之间的结合。

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  • BDP 630/650 X NHS ester

BDP 630/650 X NHS ester

标签：NHS esters Fluorescence polarization Microscopy BDP 630/650 Protein labeling
货号 规格 价格 货期
15420 1 mg 110.00$ in stock
25420 5 mg 210.00$ in stock
45420 25 mg 410.00$ in stock
55420 50 mg 695.00$ in stock
65420 100 mg 1190.00$ in stock

BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.

This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.

BDP 630/650 是一种硼二吡咯亚甲基荧光团，具有高摩尔消光系数、出色的量子产率和相对较长的激发态寿命。 因此，这种荧光团可用于荧光偏振测定，以检测分子之间的结合。

这是一种胺反应性 NHS 酯。 它在荧光团和反应基团之间包含一个氨基己酰基接头。

BDP 630/650 X NHS ester BDP 630/650 X NHS ester BDP 630/650 X NHS ester BDP 630/650 X NHS ester

Absorption and emission spectra of BDP 630/650

BDP 630/650 X NHS ester

BDP 630/650 X NHS ester相关产品

BDP TR X NHS ester

BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.

BDP TR X NHS 酯是 BDP TR 的衍生物，含有一个基于氨基己酸 (C6) 的长接头。 该染料具有接近ROX的吸收和发射。 NHS 酯功能可用于与蛋白质和肽的结合。

FAM tetrazine, 5-isomer

FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.

FAM 四嗪（甲基四嗪）用于与反式环辛烯和环丙烯共轭。 荧光素的纯 5-异构体。

dsGreen for Real-Time PCR, 100×

Stock solution of dsGreen for Real-Time PCR, 100×

用于实时 PCR 的 dsGreen 储备溶液，100×

General properties

Appearance: dark colored solid
Mass spec M+ increment: 545.2
Molecular weight: 660.5
CAS number: 2213445-35-1; 380367-48-6
Molecular formula: C33H31N4BF2O6S
IUPAC name: Succinimidyl-6-[2-(p-{(E)-2-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]ethenyl}phenoxy)acetylamino]hexanoate
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 628
ε, L⋅mol−1⋅cm−1: 97000
Emission maximum, nm: 642
Fluorescence quantum yield: 0.91
CF260: 0.029
CF280: 0.035

相关文献：

  1. Grätz, L.; Tropmann, K.; Bresinsky, M.; Müller, C.; Bernhardt, G.; Pockes, S. NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells. Scientific Reports, 2020, 10, 13288. doi: 10.1038/s41598-020-70332-3
  2. Gruber, C.G.; Pegoli, A.; Müller, C.; Grätz, L.; She, X.; Keller, M. Differently fluorescence-labelled dibenzodiazepinone-type muscarinic acetylcholine receptor ligands with high M2R affinity. RSC Medicinal Chemistry, 2020, 11(7), 823–832. doi: 10.1039/D0MD00137F
  3. Zhong, X.; Shtukenberg, A.G.; Liu, M.; Olson, I.A.; Weck, M.; Ward, M.D.; Kahr, B. Dislocation Generation by Microparticle Inclusions. Crystal Growth & Design, 2019, 19(11), 6649–6655. doi: 10.1021/acs.cgd.9b01041

BDP 630/650 X NHS ester

BDP 581/591 NHS ester

BDP 581/591 NHS ester,BDP 581/591 是一种硼二吡咯亚甲基染料，具有相对较长的荧光寿命和两个光子激发截面。 它可用于荧光偏振测定。 由于二烯系统的存在，它可以与活性氧（ROS）发生反应并产生荧光变化。

  • Products
  • Reactive dyes
  • Dye NHS esters
  • BDP 581/591 NHS ester

BDP 581/591 NHS ester

标签：NHS esters Fluorescence polarization BDP 581/591 Protein labeling
货号 规格 价格 货期
16420 1 mg 110.00$ in stock
26420 5 mg 210.00$ in stock
46420 25 mg 410.00$ in stock
56420 50 mg 695.00$ in stock
66420 100 mg 1190.00$ in stock

BDP 581/591 is a borondipyrromethene dye that has relatively long fluorescence lifetime and two photon excitation cross section. It is useful for fluorescence polarization assays. Due to the presence of a diene system, it can react with reactive oxygen species (ROS) with a change of fluorescence.

This is the NHS ester derivative for the conjugation with primary and secondary amine groups of proteins, peptides, and other molecules.

BDP 581/591 是一种硼二吡咯亚甲基染料，具有相对较长的荧光寿命和两个光子激发截面。 它可用于荧光偏振测定。 由于二烯系统的存在，它可以与活性氧（ROS）发生反应并产生荧光变化。

这是用于与蛋白质、肽和其他分子的伯胺和仲胺基团结合的 NHS 酯衍生物。

BDP 581/591 NHS ester BDP 581/591 NHS ester BDP 581/591 NHS ester BDP 581/591 NHS ester

Absorption and emission spectra of BDP 581/591

BDP 581/591 NHS ester

BDP 581/591 NHS ester相关产品

BDP TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.

BDP TMR 的胺活性 NHS 酯，用于 TAMRA 通道的明亮硼二吡咯亚甲基染料，特别适用于荧光偏振测定。

Cyanine5.5 carboxylic acid

Cy5.5 unactivated free acid cyanine dye.

Cy5.5 未活化的游离酸性花青染料。

Cyanine7 NHS ester

Amine reactive NHS ester of near infrared dye Cyanine7.

近红外染料Cyanine7的胺活性NHS酯。

General properties

Appearance: dark colored solid
Mass spec M+ increment: 374.1
Molecular weight: 489.28
CAS number: 150152-70-8
Molecular formula: C26H22N3BF2O4
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 585
ε, L⋅mol−1⋅cm−1: 104000
Emission maximum, nm: 594
Fluorescence quantum yield: 0.83
CF260: 0.06
CF280: 0.04

相关文献：

  1. Brachi, G.; Ruiz-Ramírez, J.; Dogra, P.; Wang, Z.; Cristini, V.; Ciardelli, G.; Rostomily, R.C.; Ferrari, M.; Mikheev, A.M.; Blanco, E.; Mattu, C. Intratumoral injection of hydrogel-embedded nanoparticles enhances retention in glioblastoma. Nanoscale, 2020, 12(46), 23838–23850. doi: 10.1039/d0nr05053a
  2. Tabe, H.; Sukenobe, K.; Kondo, T.; Sakurai, A.; Maruo, M.; Shimauchi, A.; Hirano, M.; Uno, S.-N.; Kamiya, M.; Urano, Y.; Matsushita, M.; Fujiyoshi, S. Cryogenic Fluorescence Localization Microscopy of Spectrally Selected Individual FRET Pairs in a Water Matrix. The Journal of Physical Chemistry B, 2018, 122(27), 6906–6911. doi: 10.1021/acs.jpcb.8b03977

Protein labeling buffer (with THPTA), 1.5x

Protein labeling buffer (with THPTA), 1.5x,催化缓冲液适用于通过 Cu(I) 催化的叠氮化物-炔烃环加成 (CuAAC) 将叠氮化物或炔烃修饰的蛋白质与带有炔烃或叠氮化物的染料偶联。 THPTA 配体由于催化活性 Cu (I) 的稳定性而加快了反应速率。

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  • Protein labeling buffer (with THPTA), 1.5x

Protein labeling buffer (with THPTA), 1.5x

标签：Protein labeling
货号 规格 价格 货期
B5150 1 mL 30.00$ in stock
K5150 10 mL 120.00$ in stock
S5150 50 mL 490.00$ in stock

Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.

Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine

催化缓冲液适用于通过 Cu(I) 催化的叠氮化物-炔烃环加成 (CuAAC) 将叠氮化物或炔烃修饰的蛋白质与带有炔烃或叠氮化物的染料偶联。 THPTA 配体由于催化活性 Cu (I) 的稳定性而加快了反应速率。 此外，水溶性 THPTA 的存在允许蛋白质标记在水溶液中运行，并通过螯合游离铜，最大限度地减少 ROS（活性氧）的产生和蛋白质的不良损伤。 氨基胍可防止反应性醛（脱氢抗坏血酸水解产物）与精氨酸、N 末端半胱氨酸和赖氨酸侧链连接。 即用型 1.5x 缓冲液提供执行 CuAAC 反应所需的所有试剂，但用于将 Cu (II) 还原为催化活性 Cu (I) 的试剂除外。 作为还原剂，我们推荐使用抗坏血酸。

缓冲液成分：Cu (II)、醋酸三乙铵缓冲液 pH 6.8、THPTA 配体、氨基胍

Protein labeling buffer (with THPTA), 1.5x Protein labeling buffer (with THPTA), 1.5x Protein labeling buffer (with THPTA), 1.5x

Protein labeling buffer (with THPTA), 1.5x相关产品

Alkyne-PEG4-Maleimide

Water soluble, heterobifunctional hydrophilic linker with a length of 14 Å, containing maleimide for thiol labeling, and alkyne for Click chemistry. An ideal reagent to attach an alkyne moiety to a protein.

水溶性异双功能亲水接头，长度为 14 Å，包含用于硫醇标记的马来酰亚胺和用于 Click 化学的炔烃。 将炔烃部分连接到蛋白质上的理想试剂。

Alkyne NHS ester (hexynoic acid NHS ester)

Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.

炔烃 NHS 酯，用于标记具有炔烃基团的生物分子，用于铜催化的点击化学。

AmdU (5-azidomethyl-2′-deoxyuridine)

Azidomethyldeoxyuridine (AmdU) is an azide containing nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain promoted Click chemistry.

叠氮甲基脱氧尿苷 (AmdU) 是一种含有核苷的叠氮化物，可通过细胞聚合酶整合到新生 DNA 中。 以这种方式连接到 DNA 上的叠氮基可以随后通过铜催化或应变促进的点击化学被炔烃或环炔烃修饰。

General properties

MSDS: Download
Product specifications

Cy5 NHS ester minimal dye

Cy5 NHS ester minimal dye,用于蛋白质标记的 Cy5 最小染料，Cy5® 最小染料的类似物。该试剂经过专门量化，可用于 2D 蛋白质组学。 每个包装包含指定数量的 NHS 酯，数量变化在 10% 以内。

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  • Cyanine5 NHS ester minimal dye

Cyanine5 NHS ester minimal dye

标签：Cy5,NHS esters,Protein labeling
货号 规格 价格 货期
1C041 5 nmol 85.00$ in stock
2C041 10 nmol 140.00$ in stock
3C041 25 nmol 210.00$ in stock

Cyanine5 minimal dye for protein labeling, an analog of Cy5® minimal dye.

This reagent is specially quantified for the use in 2D proteomics. Each package contains specified amount of NHS ester with quantity variation within 10%.

用于蛋白质标记的 Cy5 最小染料，Cy5® 最小染料的类似物。

该试剂经过专门量化，可用于 2D 蛋白质组学。 每个包装包含指定数量的 NHS 酯，数量变化在 10% 以内。

Cyanine5 NHS ester minimal dye Cyanine5 NHS ester minimal dye Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye

相关产品

Cyanine3 alkyne

Cyanine3 alkyne is a fluorescent dye alkyne for Click Chemistry.
Cy3 炔烃是一种用于 Click Chemistry 的荧光染料炔烃。

Cyanine5 alkyne

Cyanine5 alkyne is an alkyne dye for Click Chemistry.
Cy5 炔烃是一种用于 Click Chemistry 的炔烃染料。

BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
氨基反应性 BDP FL，一种非常明亮且光稳定的荧光素通道染料。

General properties

Appearance: dark blue solid
Quality control: NMR 1H, HPLC-MS, functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
Emission maximum, nm: 662
CF260: 0.03
CF280: 0.04

相关文献：

  1. Lobov, A.A.; Babkina, I.Y.; Danilov, L.G.; Masharskiy, A.E.; Predeus, A.V.; Mikhailova, N.A.; Granovitch, A.I.; Maltseva, A.L. Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of Littorina fabalis and L. obtusata. Biology, 2021, 10(11), 1087. doi: 10.3390/biology10111087
  2. Nixon, B.; Anderson, A.L.; Bromfield, E.G.; Martin, J.H.; Cafe, S.L.; Skerrett-Byrne, D.A.; Dun, M.D.; Eamens, A.L.; De Iuliis, G.N.; Johnston, S.D. Post-testicular sperm maturation in the saltwater crocodile Crocodylus porosus: assessing the temporal acquisition of sperm motility. Reproduction, Fertility, and Development, 2021, 33(9), 530–539. doi: 10.1071/RD20204
  3. Shikov, A.E.; Malovichko, Y.V.; Lobov, A.A.; Belousova, M.E.; Nizhnikov, A.A.; Antonets, K.S. The Distribution of Several Genomic Virulence Determinants Does Not Corroborate the Established Serotyping Classification of Bacillus thuringiensis. International Journal of Molecular Sciences, 2021, 22(5), 2244. doi: 10.3390/ijms22052244
  4. Serteyn, L.; Bosquee, E.; Delatour, A.; Rubio-Melendez, M.E.; Vega-Muñoz, K.V.; Ramirez, C.C.; Francis, F. Multi-approach comparative study of the two most prevalent genotypes of pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) in Chile. Entomological Science, 2021, 24(1), 55–67. doi: 10.1111/ens.12436

Cy® is a trademark of GE Healthcare.

Purchase of these products does not imply transfer of license to perform methods patented by Carnegie Mellon University covered by US patents 6,043,025, 6,127,134, 6,246,190, 7566,544, and 7,598,047 “Difference gel electrophoresis using matched multiple dyes” and family members in Australia, Canada, Europe and Japan.
购买这些产品并不意味着转让执行卡内基梅隆大学专利方法的许可，这些方法受美国专利 6,043,025、6,127,134、6,246,190、7566,544 和 7,598,047“使用匹配的多种染料的差异凝胶电泳”和澳大利亚、加拿大的家庭成员的保护 ，欧洲和日本。

Cyanine3 NHS ester minimal dye

Cyanine3 NHS ester minimal dye,用于蛋白质标记的 Cy3 NHS 酯染料和 Cy3® 最小染料的类似物。该试剂经过特殊量化——每包含有指定量的 NHS 酯，数量变化在 10% 以内。

  • Products
  • Miscellaneous reagents
  • Proteomic reagents
  • Cyanine3 NHS ester minimal dye

Cyanine3 NHS ester minimal dye

标签：Cyanine3,NHS esters,Protein labeling
Cat. # Quantity Price Lead time
1B041 5 nmol 85.00$ in stock

2B041 10 nmol 140.00$ in stock

3B041 25 nmol 210.00$ in stock

Cyanine3 NHS ester dye for protein labeling, and analog of Cy3® minimal dye.

This reagent is specially quantified – each package contains specified amount of NHS ester with quantity variation within 10%.

用于蛋白质标记的 Cy3 NHS 酯染料和 Cy3® 最小染料的类似物。

该试剂经过特殊量化——每包含有指定量的 NHS 酯，数量变化在 10% 以内。

Cy3 NHS ester minimal dye相关产品

Cyanine5.5 carboxylic acid

Cy5.5 unactivated free acid cyanine dye.

Cy5.5 未活化的游离酸性花青染料。

Cyanine3 NHS ester minimal dye Add this product to your cart and
get free express delivery

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.

Cy5 NHS 酯最小染料用于蛋白质标记。

Cyanine3 NHS ester minimal dye Add this product to your cart and
get free express delivery

Sulfo-Cyanine7.5 NHS ester

A water soluble near infrared emitting dye for in vivo imaging applications. Sulfo-Cyanine7.5 is an analog of indocyanine green (ICG) possessing higher emission quantum yield, and containing a linker arm.

一种用于体内成像应用的水溶性近红外发射染料。 Sulfo-Cy7.5 是吲哚菁绿 (ICG) 的类似物，具有更高的发射量子产率，并包含一个连接臂。

Cyanine3 NHS ester minimal dye Add this product to your cart and
get free express delivery

General properties

Appearance: red solid
Quality control: NMR 1H, HPLC-MS, functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
Emission maximum, nm: 570
CF260: 0.04
CF280: 0.09

相关文献：

  1. Lobov, A.A.; Babkina, I.Y.; Danilov, L.G.; Masharskiy, A.E.; Predeus, A.V.; Mikhailova, N.A.; Granovitch, A.I.; Maltseva, A.L. Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of Littorina fabalis and L. obtusata. Biology, 2021, 10(11), 1087. doi: 10.3390/biology10111087
  2. Nixon, B.; Anderson, A.L.; Bromfield, E.G.; Martin, J.H.; Cafe, S.L.; Skerrett-Byrne, D.A.; Dun, M.D.; Eamens, A.L.; De Iuliis, G.N.; Johnston, S.D. Post-testicular sperm maturation in the saltwater crocodile Crocodylus porosus: assessing the temporal acquisition of sperm motility. Reproduction, Fertility, and Development, 2021, 33(9), 530–539. doi: 10.1071/RD20204
  3. Shikov, A.E.; Malovichko, Y.V.; Lobov, A.A.; Belousova, M.E.; Nizhnikov, A.A.; Antonets, K.S. The Distribution of Several Genomic Virulence Determinants Does Not Corroborate the Established Serotyping Classification of Bacillus thuringiensis. International Journal of Molecular Sciences, 2021, 22(5), 2244. doi: 10.3390/ijms22052244
  4. Serteyn, L.; Bosquee, E.; Delatour, A.; Rubio-Melendez, M.E.; Vega-Muñoz, K.V.; Ramirez, C.C.; Francis, F. Multi-approach comparative study of the two most prevalent genotypes of pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) in Chile. Entomological Science, 2021, 24(1), 55–67. doi: 10.1111/ens.12436

Cy® is a trademark of GE Healthcare.

Purchase of these products does not imply transfer of license to perform methods patented by Carnegie Mellon University and covered by US patents 6,043,025, 6,127,134, 6,246,190, 7566,544, and 7,598,047 “Difference gel electrophoresis using matched multiple dyes” and family members in Australia, Canada, Europe and Japan.

Cyanine3 NHS ester minimal dye

购买这些产品并不意味着转让执行卡内基梅隆大学专利的方法并受美国专利 6,043,025、6,127,134、6,246,190、7566,544 和 7,598,047“使用匹配的多种染料的差异凝胶电泳”和澳大利亚的家庭成员的许可， 加拿大、欧洲和日本。

Cy2 NHS ester minimal dye

Cy2 NHS ester minimal dye,用于蛋白质标记的 Cy2 最小染料，相当于 Cy2® NHS 酯最小染料。该试剂经过特殊量化——每包含有指定量的 NHS 酯，数量变化在 10% 以内。

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  • Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye

标签：NHS esters,Protein labeling
货号 规格 价格 货期
1A041 5 nmol 85.00$ in stock
2A041 10 nmol 140.00$ in stock
3A041 25 nmol 210.00$ in stock

Cyanine2 minimal dye for protein labeling, an equivalent of Cy2® NHS ester minimal dye.

This reagent is specially quantified – each package contains specified amount of NHS ester with quantity variation within 10%.

用于蛋白质标记的 Cy2 最小染料，相当于 Cy2® NHS 酯最小染料。

该试剂经过特殊量化——每包含有指定量的 NHS 酯，数量变化在 10% 以内。

Cyanine2 NHS ester minimal dye Cyanine2 NHS ester minimal dye Cyanine2 NHS ester minimal dye、

Cy2 NHS ester minimal dye相关产品

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5 azide 是一种用 Cy5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。

dsGreen Gel Staining Solution, 10000×

dsGreen for staining dsDNA in gels.

dsGreen 用于在凝胶中染色 dsDNA。

Cyanine5.5 azide

Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.

用于 Click Chemistry 的远红/近红外荧光 Cy5.5 染料衍生物。

General properties

Appearance: yellow solid 黄色固体
Quality control: NMR 1H, HPLC-MS, functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
Emission maximum, nm: 510

相关文献：

  1. Lobov, A.A.; Babkina, I.Y.; Danilov, L.G.; Masharskiy, A.E.; Predeus, A.V.; Mikhailova, N.A.; Granovitch, A.I.; Maltseva, A.L. Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of Littorina fabalis and L. obtusata. Biology, 2021, 10(11), 1087. doi: 10.3390/biology10111087
  2. Shikov, A.E.; Malovichko, Y.V.; Lobov, A.A.; Belousova, M.E.; Nizhnikov, A.A.; Antonets, K.S. The Distribution of Several Genomic Virulence Determinants Does Not Corroborate the Established Serotyping Classification of Bacillus thuringiensis. International Journal of Molecular Sciences, 2021, 22(5), 2244. doi: 10.3390/ijms22052244
  3. Serteyn, L.; Bosquee, E.; Delatour, A.; Rubio-Melendez, M.E.; Vega-Muñoz, K.V.; Ramirez, C.C.; Francis, F. Multi-approach comparative study of the two most prevalent genotypes of pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae) in Chile. Entomological Science, 2021, 24(1), 55–67. doi: 10.1111/ens.12436
  4. Tamošiūnė, I.; Gelvonauskienė, D.; Haimi, P.; Mildažienė, V.; Koga, K.; Shiratani, M.; Baniulis, D. Cold Plasma Treatment of Sunflower Seeds Modulates Plant-Associated Microbiome and Stimulates Root and Lateral Organ Growth. Frontiers in Plant Science, 2020, 11, 568924. doi: 10.3389/fpls.2020.568924

Cy2® is a trademark of GE Healthcare.

Purchase of these products does not imply transfer of license to perform methods patented by Carnegie Mellon University and covered by US patents 6,043,025, 6,127,134, 6,246,190, 7566,544, and 7,598,047 “Difference gel electrophoresis using matched multiple dyes” and family members in Australia, Canada, Europe and Japan.

购买这些产品并不意味着转让执行卡内基梅隆大学专利的方法并受美国专利 6,043,025、6,127,134、6,246,190、7566,544 和 7,598,047“使用匹配的多种染料的差异凝胶电泳”和澳大利亚的家庭成员的许可， 加拿大、欧洲和日本。

DMSO, labeling grade

DMSO, labeling grade,DMSO 是一种广泛用于生物偶联的极性非质子溶剂。 这种溶剂可以被认为是点击反应非常重要的助溶剂，可以用水溶性差的荧光染料标记各种物体。 当疏水性标记用于蛋白质标记时，或者当标记分子需要在反应前被等分时，DMSO 也被推荐作为反应混合物中的辅助溶剂。

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  • DMSO, labeling grade

DMSO, labeling grade

标签：Click chemistry,Protein labeling
货号 规格 价格 货期
15050 1 mL 10.00$ in stock

DMSO is a polar aprotic solvent widely used in bioconjugation. This solvent can be considered as very important co-solvent for Click reactions to label various objects with poorly water-soluble fluorescent dyes. DMSO is also recommended as an auxiliary solvent in the reaction mixture when a hydrophobic label is used for protein labeling, or when a label molecule needs to be aliquoted before the reaction.

DMSO 是一种广泛用于生物偶联的极性非质子溶剂。 这种溶剂可以被认为是点击反应非常重要的助溶剂，可以用水溶性差的荧光染料标记各种物体。 当疏水性标记用于蛋白质标记时，或者当标记分子需要在反应前被等分时，DMSO 也被推荐作为反应混合物中的辅助溶剂。

DMSO, labeling grade DMSO, labeling grade DMSO, labeling grade DMSO, labeling grade

DMSO, labeling grade相关产品

FAM azide, 6-isomer

FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.

FAM（荧光素）叠氮化物，6 异构体是 Click Chemistry 中常用的荧光染料叠氮化物。

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5 azide 是一种用 Cy5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。

BDP 581/591 hydrazide

BDP 581/591 is a multi-purpose dye with bright emission. This is a hydrazide dye for the conjugation with aldehydes and ketones.

BDP 581/591 是一种具有明亮发射的多用途染料。 这是一种用于与醛和酮结合的酰肼染料。

General properties

Appearance: colorless liquid
Molecular weight: 78.14
CAS number: 67-68-5
Molecular formula: C2H6OS
IUPAC name: dimethylsulfoxide
Solubility: miscible with water, many organic solvents
Quality control: GCMS (98+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

DMF (dimethylformamide), labeling grade

DMF (dimethylformamide), labeling grade,DMF（二甲基甲酰胺）是使用 NHS 酯标记反应的推荐溶剂。 然而，当 DMF 储存在空气中时，它会吸收水分缓慢变质，并释放出二甲胺。 这些杂质迅速破坏NHS酯功能，降低标记效率，甚至使标记反应失败。

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  • DMF (dimethylformamide), labeling grade

DMF (dimethylformamide), labeling grade

标签：Protein labeling
货号 规格 价格 货期
13050 1 mL 10.00$ in stock

DMF (dimethylformamide) is a recommended solvent for labeling reactions using NHS esters. However, when DMF is stored in air, it deteriorates slowly absorbing moisture, and releasing dimethylamine. These impurities rapidly destroy NHS ester function, decrease labeling efficiency, and even make labeling reaction fail.

Dimethylformamide solvent requires special drying and preparation, which is often not available in all laboratories.

This product contains ready-to use solvent which is guaranteed to be useable for the labeling. Tubes with high quality, dry, and amine-free DMF are packed under argon.

DMF（二甲基甲酰胺）是使用 NHS 酯标记反应的推荐溶剂。 然而，当 DMF 储存在空气中时，它会吸收水分缓慢变质，并释放出二甲胺。 这些杂质迅速破坏NHS酯功能，降低标记效率，甚至使标记反应失败。

二甲基甲酰胺溶剂需要特殊的干燥和制备，这通常不是所有实验室都可以使用的。

本产品含有即用型溶剂，保证可用于标签。 具有高质量、干燥和无胺 DMF 的管子在氩气下包装。

DMF (dimethylformamide), labeling grade DMF (dimethylformamide), labeling grade DMF (dimethylformamide), labeling grade

DMF (dimethylformamide), labeling grade相关产品

FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。

Sulfo-Cyanine7 amine

Water-soluble NIR dye amine, sulfonated derivative of Cyanine7 dye, for the modification via enzymatical transamination.

水溶性 NIR 染料胺，Cyanine7 染料的磺化衍生物，用于通过酶促氨基转移进行修饰。

ROX reference dye for qPCR

ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.

ROX 是用于 qPCR 扩增的被动参考染料。 该染料可用于标准化 qPCR 中报告染料的荧光强度。

General properties

Appearance: colorless liquid
Molecular weight: 73.09
CAS number: 68-12-2
Molecular formula: C3H7NO
IUPAC name: N,N-dimethylformamide
Solubility: miscible with water and many organic solvents
Quality control: GCMS (99+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

相关文献：

  1. Paul, M.; Tscheuschner, G.; Herrmann, S.; Weller, M.G. Fast Detection of 2,4,6-Trinitrotoluene (TNT) at ppt Level by a Laser-Induced Immunofluorometric Biosensor. Biosensors, 2020, 10(8), 89. doi: 10.3390/bios10080089
  2. Gu, R.; Oweida, T.; Yingling, Y.G.; Chilkoti, A.; Zauscher, S. Enzymatic synthesis of nucleobase-modified single-stranded DNA offers tunable resistance to nuclease degradation. Biomacromolecules, 2018, 19(8), 3525–3535. doi: 10.1021/acs.biomac.8b00816

DBCO NHS ester

DBCO NHS ester,二苯并环辛炔 (ADIBO, DBCO) 是对应变促进炔烃叠氮化物环加成 (spAAC) 反应性最强的环炔烃之一 – 一种无铜点击化学反应。

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  • DBCO NHS ester

DBCO NHS ester

标签：NHS esters, Alkynes, Copper free Click chemistry, Cycloalkyne, Protein labeling, Cross linking reagents
货号 规格 价格 货期
34720 10 mg 60.00$ in stock
44720 25 mg 95.00$ in stock
54720 50 mg 145.00$ in stock
64720 100 mg 210.00$ in stock
94720 1 g 1190.00$ in stock
K4720 5 g 3570.00$ in stock
N4720 25 g please inquire 21 days

Dibenzocyclooctyne (ADIBO, DBCO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC) – a copper free Click chemistry reaction.

This is an amine-reactive NHS ester that provides easy attachment of the reactive moiety to almost any primary or secondary amine group, such as that of protein, peptide, or small molecule amine.

DBCO reacts instantly with azides. The rate of the reaction is much higher than that of copper-catalyzed reaction, and reactions with many other cyclooctynes. Unlike some other cyclooctynes, DBCO does not react with tetrazines – this allows to carry out orthogonal conjugation of azides with DBCO, and trans-cyclooctenes with tetrazines.

二苯并环辛炔 (ADIBO, DBCO) 是对应变促进炔烃叠氮化物环加成 (spAAC) 反应性最强的环炔烃之一 – 一种无铜点击化学反应。

这是一种胺反应性 NHS 酯，可将反应性部分轻松连接到几乎任何伯胺或仲胺基团，例如蛋白质、肽或小分子胺的基团。

DBCO 立即与叠氮化物发生反应。 该反应的速率远高于铜催化的反应，以及与许多其他环辛炔的反应。 与其他一些环辛炔不同，DBCO 不与四嗪反应 – 这允许叠氮化物与 DBCO 以及反式环辛烯与四嗪的正交共轭。

DBCO NHS ester相关产品

FAM azide, 6-isomer

FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for Click Chemistry.

FAM（荧光素）叠氮化物，6 异构体是 Click Chemistry 中常用的荧光染料叠氮化物。

Sulfo-Cyanine5.5 carboxylic acid

Water soluble far red fluorescent dye sulfo-Cyanine5.5, free non-activated carboxylic acid. Useful for the calibration, and as a non-reactive control.

水溶性远红荧光染料sulfo-Cy5.5，游离非活化羧酸。 用于校准，并作为非反应控制。

Cyanine3.5 NHS ester

Cyanine3.5 dye NHS ester derivative.

Cy3.5染料NHS酯衍生物。

General properties

Appearance: off white solid
Mass spec M+ increment: 315.1
Molecular weight: 430.45
CAS number: 1384870-47-6
Molecular formula: C25H22N2O5
IUPAC name: 6-{2-Azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl}-6-oxohexanamide
Solubility: good in DCM, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

相关文献：

  1. Adams, M.R.; Moody, C.T.; Sollinger, J.L.; Brudno, Y. Extracellular Matrix-Anchored Click Motifs for Specific Tissue Targeting. Molecular Pharmaceutics, 2020, 17(2), 392–403. doi: 10.1021/acs.molpharmaceut.9b00589
  2. Alshanski, I.; Blaszkiewicz, J.; Mervinetsky, E.; Rademann, J.; Yitzchaik, S.; Hurevich, M. Sulfation Patterns of Saccharides and Heavy Metal Ions Binding. Chemistry, 2019, 25(52), 12083–12090. doi: 10.1002/chem.201901538
  3. Andersen, V.L.; Vinther, M.; Kumar, R.; Ries, A.; Wengel, J.; Nielsen, J.S.; Kjems, J. A self-assembled, modular nucleic acid-based nanoscaffold for multivalent theranostic medicine. Theranostics, 2019, 9(9), 2662–2677. doi: 10.7150/thno.32060

Alkyne NHS ester (hexynoic acid NHS ester)

Alkyne NHS ester (hexynoic acid NHS ester),用于生物分子修饰的炔烃 NHS 酯。 用于铜催化点击化学反应的炔基在天然分子中几乎从未遇到过。 然而，这种 NHS 酯允许将炔基连接到氨基上，氨基在自然界中普遍存在，并且存在于蛋白质、肽、合成氨基 DNA 和许多小分子中。 随后可以通过铜催化的点击化学修饰和缀合炔基。

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  • Non-fluorescent alkynes
  • Alkyne NHS ester (hexynoic acid NHS ester)

Alkyne NHS ester (hexynoic acid NHS ester)

标签：NHS esters, Alkynes, Protein labeling, Cross linking reagents
货号 规格 价格 货期
41720 25 mg 95.00$ in stock
51720 50 mg 195.00$ in stock
61720 100 mg 290.00$ in stock
91720 1 g please inquire in stock

Alkyne NHS ester for the modification of biomolecules. Alkyne groups for copper catalyzed Click Chemistry reaction are nearly never encountered in natural molecules. However, this NHS ester allows to attach alkyne groups to amino groups, which are ubiquitous in nature, and which are present in proteins, peptides, synthetic amino-DNA and numerous small molecules. Alkyne groups can be subsequently modified and conjugated by Copper catalyzed Click chemistry.

This reagent is a derivative of hexynoic acid.

用于生物分子修饰的炔烃 NHS 酯。 用于铜催化点击化学反应的炔基在天然分子中几乎从未遇到过。 然而，这种 NHS 酯允许将炔基连接到氨基上，氨基在自然界中普遍存在，并且存在于蛋白质、肽、合成氨基 DNA 和许多小分子中。 随后可以通过铜催化的点击化学修饰和缀合炔基。

该试剂是己炔酸的衍生物。

Alkyne NHS ester (hexynoic acid NHS ester)相关产品

EdU (5-ethynyl-2′-deoxyuridine)

EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.

EdU 是一种核苷，可通过细胞酶掺入复制的 DNA 中。 之后，细胞中所含的 DNA 可以通过与荧光染料叠氮化物的 Click 化学反应进行显色，以揭示细胞增殖情况。

AF488 NHS ester

AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.

AF488 NHS 酯，一种用于绿色通道的明亮、光稳定性和亲水性荧光染料。

Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.

芘 NHS 酯，用于用芘残基标记生物分子。 芘是一种形成准分子的荧光邻近探针，也是石墨固定的锚定基团。

General properties

Appearance: yellowish oil / off white crystals 淡黄色油状物/类白色结晶
Mass spec M+ increment: 94.0
Molecular weight: 209.20
CAS number: 906564-59-8
Molecular formula: C10H11NO4
IUPAC name: 5-​Hexynoic acid, 2,​5-​dioxo-​1-​pyrrolidinyl ester
Solubility: good in organic solvents (DMF, DMSO, DCM)
Quality control: NMR 1H 95+%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

相关文献：

  1. Zanon, P.R.A.; Yu, F.; Musacchio, P.; Lewald, L.; Zollo, M.; Krauskopf, K.; Mrdović, D.; Raunft, P.; Maher, T.E.; Cigler, M.; Chang, C.; Lang, K.; Toste, F.D.; Nesvizhskii, A.I.; Hacker, S.M. Profiling the proteome-wide selectivity of diverse electrophiles. ChemRxiv, preprint. doi: 10.33774/chemrxiv-2021-w7rss-v2
  2. Kleinpenning, F.; Steigenberger, B.; Wu, W.; Heck, A.J.R. Fishing for newly synthesized proteins with phosphonate-handles. Nature Communications, 2020, 11, 3244. doi: 10.1038/s41467-020-17010-0

Azidobutyric acid NHS ester

Azidobutyric acid NHS ester,使用该试剂将您的蛋白质和肽转化为 Click Chemistry 反应形式。

  • Products
  • Miscellaneous reagents
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  • Azidobutyric acid NHS ester

Azidobutyric acid NHS ester

标签：NHS esters, Protein labeling, Cross linking reagents
货号 规格 价格 货期
53720 50 mg 60.00$ in stock
63720 100 mg 95.00$ in stock
73720 1 g please inquire in stock
83720 5 g please inquire in stock

Convert your proteins and peptides into Click Chemistry reactive form with this reagent.

While Click Chemistry involves reaction between terminal alkyne and azide, both azides and alkynes are very uncommon in nature. However, there are reagents to attach these fragments to abundant amino groups which are ubiquitous in the world of biomolecules. This azido-NHS ester is designed for the conversion of proteins, peptides, amino-DNA, and other amines into Click Chemistry reactive azides.

使用该试剂将您的蛋白质和肽转化为 Click Chemistry 反应形式。

虽然 Click Chemistry 涉及末端炔烃和叠氮化物之间的反应，但叠氮化物和炔烃在自然界中都非常罕见。 然而，有一些试剂可以将这些片段连接到丰富的氨基上，这些氨基在生物分子的世界中无处不在。 这种叠氮基-NHS 酯设计用于将蛋白质、肽、氨基-DNA 和其他胺转化为 Click Chemistry 反应性叠氮化物。

Azidobutyric acid NHS ester相关产品

Cyanine7 carboxylic acid

Non-reactive, near-infrared fluorescent dye.

非反应性近红外荧光染料。

TAMRA azide, 5-isomer

TAMRA fluorescent dye azide for Click chemistry.

用于点击化学的 TAMRA 荧光染料叠氮化物。

Cyanine5 azide

Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.

Cy5 azide 是一种用 Cy5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。

General properties

Appearance: colorless solid 无色固体
Mass spec M+ increment: 111.0
Molecular weight: 226.19
CAS number: 943858-70-6
Molecular formula: C8H10N4O4
IUPAC name: Butanoic acid, 4-​azido-​, 2,​5-​dioxo-​1-​pyrrolidinyl ester
Solubility: soluble in organic solvents (DMF, DMSO)
Quality control: NMR 1H (95%), HPLC
Storage conditions: Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存：-20°C 收货后 12 个月。 运输：在室温下长达 3 周。 干燥。
MSDS: Download
Product specifications

相关文献：

  1. Wang, Y.; Yao, J.; Cai, L.; Liu, T.; Wang, X.; Zhang, Y.; Zhou, Z.; Li, T.; Liu, M.; Lai, R.; Liu, X. Bone-targeted extracellular vesicles from mesenchymal stem cells for osteoporosis therapy. Research Square, preprint. doi: 10.21203/rs.3.rs-18279/v1
  2. Kleinpenning, F.; Steigenberger, B.; Wu, W.; Heck, A.J.R. Fishing for newly synthesized proteins with phosphonate-handles. Nature Communications, 2020, 11, 3244. doi: 10.1038/s41467-020-17010-0
  3. Blanchard, A.T.; Bazrafshan, A.S.; Yi, J.; Eisman, J.T.; Yehl, K.M.; Bian, T.; Mugler, A.; Salaita, K. Highly Polyvalent DNA Motors Generate 100+ pN of Force via Autochemophoresis. Nano Letters, 2019, 19(10), 6977–6986. doi: 10.1021/acs.nanolett.9b02311
  4. Hou, W.; Li, Y.; Kang, W.; Wang, X.; Wu, X.; Wang, S.; Liu, F. Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking. Scientific Reports, 2019, 9, 1307. doi: 10.1038/s41598-018-37789-9

TAMRA NHS ester, 5-isomer

TAMRA NHS ester, 5-isomer,TAMRA（四甲基罗丹明）是罗丹明系列的呫吨染料。 这种荧光团用于制备含有 TAMRA 和荧光素 (FAM) 的双标记 qPCR TaqMan 寡核苷酸探针已经有很长时间了。

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  • TAMRA NHS ester, 5-isomer

TAMRA NHS ester, 5-isomer

标签：NHS esters,Protein labeling,TAMRA (TMR, tetramethylrhodamine)
货号 规格 价格 货期
17120 1 mg – in stock
27120 5 mg 110.00$ in stock
47120 25 mg 220.00$ in stock
57120 50 mg 395.00$ in stock
67120 100 mg 480.00$ in stock

TAMRA (tetramethylrhodamine) is a xanthene dye of rhodamine series. This fluorophore is being used for quite a long time for the preparation of dual labeled qPCR TaqMan oligonucleotide probes containing TAMRA and fluorescein (FAM).

Just like many other xanthene fluorophores, TAMRA is a available as two isomers (5- and 6-isomer) which have nearly identical optical properties. This product is an isomerically pure 5-TAMRA.

TAMRA NHS is an amine reactive reagent. It can be used for the labeling of proteins, peptides, and modified oligonucleotides containing amine groups.

TAMRA（四甲基罗丹明）是罗丹明系列的呫吨染料。 这种荧光团用于制备含有 TAMRA 和荧光素 (FAM) 的双标记 qPCR TaqMan 寡核苷酸探针已经有很长时间了。

就像许多其他呫吨荧光团一样，TAMRA 可作为两种异构体（5-和 6-异构体）获得，它们具有几乎相同的光学特性。 该产品是异构纯的 5-TAMRA。

TAMRA NHS 是一种胺反应试剂。 它可用于标记蛋白质、肽和含有胺基的修饰寡核苷酸。
TAMRA NHS ester, 5-isomer TAMRA NHS ester, 5-isomer TAMRA NHS ester, 5-isomer TAMRA NHS ester, 5-isomer

Absorption and emission spectra of 5-TAMRA

TAMRA NHS ester, 5-isomer

TAMRA NHS ester, 5-isomer相关产品

Azide-PEG3-Amine

A bifunctional linker with azide and amine groups flanking PEG3 (triethylene glycol)

具有叠氮化物和胺基的双功能接头，位于 PEG3（三甘醇）的两侧

Alkyne Phosphoramidite, 5′-terminal

Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.

用于合成寡核苷酸的炔亚磷酰胺，可用于点击化学。

BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.

氨基反应性 BDP FL，一种非常明亮且光稳定的荧光素通道染料。

General properties

Appearance: dark colored solid
Molecular weight: 527.53
CAS number: 321862-17-3
Molecular formula: C29H25N3O7
IUPAC name: (2,5-dioxopyrrolidin-1-yl) 3′,6′-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylate
Solubility: good in DMF, DMSO, low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receipt at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19

Sulfo-Cy7.5 NHS ester

NHS esters,Near infrared (NIR) imaging,sulfo-Cy7.5,Protein labeling，Sulfo-Cy7.5 是一种用于近红外成像应用的近红外水溶性亲水染料。 该染料的结构和光谱类似于已被批准用于人类多年的吲哚菁绿 (ICG)。 然而，与 ICG 不同，磺基-Cy7.5 含有一个三亚甲基桥，与 ICG 相比，它增加了其量子产率，并且还具有一个连接臂，用于连接蛋白质、肽和其他分子。 该衍生物是用于修饰胺基的 NHS 酯。

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  • Sulfo-Cyanine7.5 NHS ester

Sulfo-Cy7.5 NHS ester

标签：NHS esters Near infrared (NIR) imaging sulfo-Cyanine7.5 Protein labeling
价格
货号 规格 货期
N6320 1 rxn – in stock
16320 1 mg 110.00$ in stock
26320 5 mg 290.00$ in stock
46320 25 mg 690.00$ in stock
56320 50 mg 1190.00$ in stock
66320 100 mg 1850.00$ in stock

Sulfo-Cyanine7.5 is a near infrared water soluble and hydrophilic dye for the NIR imaging applications. The structure and spectra of the dye resemble indocyanine green (ICG) that has been approved for use in humans for years. However, unlike ICG, sulfo-Cyanine7.5 contains a trimethylene bridge that increases its quantum yield compared to ICG, and also has a linker arm for its attachment to proteins, peptides, and other molecules. This derivative is an NHS ester for the modification of amine groups.

Sulfo-Cy7.5 是一种用于近红外成像应用的近红外水溶性亲水染料。 该染料的结构和光谱类似于已被批准用于人类多年的吲哚菁绿 (ICG)。 然而，与 ICG 不同，磺基-Cy7.5 含有一个三亚甲基桥，与 ICG 相比，它增加了其量子产率，并且还具有一个连接臂，用于连接蛋白质、肽和其他分子。 该衍生物是用于修饰胺基的 NHS 酯。

Sulfo-Cyanine7.5 NHS ester Sulfo-Cyanine7.5 NHS ester Sulfo-Cyanine7.5 NHS ester Sulfo-Cyanine7.5 NHS ester

Absorption and emission spectra of sulfo-Cyanine7.5

Sulfo-Cyanine7.5 NHS ester

Sulfo-Cy7.5 NHS ester相关产品

BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.

氨基反应性 BDP FL，一种非常明亮且光稳定的荧光素通道染料。

Cy3 carboxylic acid

Free Cyanine3 carboxylic acid.

游离Cy3羧酸。

Sulfo-Cyanine7.5 dicarboxylic acid

Sulfo-Cy7.5 dicarboxylic acid is a bifunctional cyanine dye with two carboxylic acid (COOH) groups. The molecule can be used to construct cross conjugates containing a near infrared emitting fluorophore.

Sulfo-Cy7.5 二羧酸是一种具有两个羧酸 (COOH) 基团的双功能花青染料。 该分子可用于构建含有近红外发射荧光团的交叉偶联物。

General properties

Appearance: dark green solid
Mass spec M+ increment: 950.2
Molecular weight: 1180.47
Molecular formula: C49H48N3K3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 778
ε, L⋅mol−1⋅cm−1: 222000
Emission maximum, nm: 797
Fluorescence quantum yield: 0.21
CF260: 0.09
CF280: 0.09

相关文献：

  1. González, M.I.; González-Arjona, M.; Santos-Coquillat, A.; Vaquero, J.; Vázquez-Ogando, E.; de Molina, A.; Peinado, H.; Desco, M.; Salinas, B. Covalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications. Biomedicines, 2021, 9, 81. doi: 10.3390/biomedicines9010081
  2. Park, B.G.; Kim, Y.J.; Min, J.H.; Cheong, T.-C.; Nam, S.H.; Cho, N.-H.; Kim, Y.K.; Lee, K.B. Assessment of Cellular Uptake Efficiency According to Multiple Inhibitors of Fe3O4-Au Core-Shell Nanoparticles: Possibility to Control Specific Endocytosis in Colorectal Cancer Cells. Nanoscale Research Letters, 2020, 15, 165. doi: 10.1186/s11671-020-03395-w
  3. Ju, Y.; Guo, H.; Yarber, F.; Edman, M.C.; Peddi, S.; Janga, S.R.; MacKay, J.A.; Hamm-Alvarez, S.F. Molecular Targeting of Immunosuppressants Using a Bi-functional Elastin-Like Polypeptide. Bioconjugate Chemistry, 2019, 30(9), 2358–2372. doi: 10.1021/acs.bioconjchem.9b00462
  4. Ruiz, A.; Bravo, D.; Duarte, A.; Adler, R.S.; Raya, J.G. Accuracy of Ultrasound-Guided versus Landmark-Guided Intra-articular Injection for Rat Knee Joints. Ultrasound in Medicine and Biology, 2019, 45(10), 2787–2796. doi: 10.1016/j.ultrasmedbio.2019.06.403

Sulfo-Cy7 NHS ester

Sulfo-Cy7 NHS ester,水溶性近红外染料 sulfo-Cy7，一种胺反应性琥珀酰亚胺酯。Sulfo-Cy7 是 Cy7® 荧光团的改进类似物，量子产率提高了 20%，光稳定性更高。 这种荧光染料对近红外成像特别有用。

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  • Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester

标签：sulfo-Cy7 NHS esters Near infrared (NIR) imaging Protein labeling
货号 规格 价格 货期
15320 1 mg 110.00$ in stock
25320 5 mg 290.00$ in stock
45320 25 mg 690.00$ in stock
55320 50 mg 1270.00$ in stock
65320 100 mg 1990.00$ in stock

Water soluble near infrared dye sulfo-Cy7, an amine-reactive succinimide ester.

Sulfo-Cy7 is an improved analog of Cy7® fluorophore with quantum yield improved by 20%, and higher photostability. This fluorescent dye is especially useful for NIR imaging.

Near infrared fluorescent imaging takes advantage of transparency of biological tissues at particular range of wavelengths. The method is non-destructive, and allows to monitor distribution of various labeled molecules in live organisms.

Sulfo-Cyanine7 NHS ester reagent allows to prepare sulfo-Cyanine7-labeled biomolecules, such as proteins, with ease. Dye labeled molecules can be subsequently used for various research and drug design related experiments.

This reagent has high water solubility, and is especially useful for the labeling of delicate proteins, and proteins prone to denaturation. Non-sulfonated Cy7 NHS ester soluble in organic phase is also available.

水溶性近红外染料 sulfo-Cy7，一种胺反应性琥珀酰亚胺酯。

Sulfo-Cy7 是 Cy7® 荧光团的改进类似物，量子产率提高了 20%，光稳定性更高。 这种荧光染料对近红外成像特别有用。

近红外荧光成像利用特定波长范围内生物组织的透明度。 该方法是非破坏性的，可以监测活生物体中各种标记分子的分布。

Sulfo-Cyanine7 NHS 酯试剂可以轻松制备磺基-Cyanine7 标记的生物分子，例如蛋白质。 染料标记的分子随后可用于各种研究和药物设计相关实验。

该试剂具有高水溶性，特别适用于标记精细蛋白质和易于变性的蛋白质。 也可提供可溶于有机相的非磺化 Cy7 NHS 酯。

Sulfo-Cyanine7 NHS ester Sulfo-Cyanine7 NHS ester Sulfo-Cyanine7 NHS ester Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 absorbance and emission spectra

Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester相关产品

AF430 NHS ester

AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.
AF430 是一种亲水性香豆素染料，发射在黄绿色范围内。 NHS 酯用于标记胺基。

Cyanine5 alkyne

Cyanine5 alkyne is an alkyne dye for Click Chemistry.
Cy5 炔烃是一种用于 Click Chemistry 的炔烃染料。

Pico488 dsDNA quantification reagent, 200x solution in DMSO

Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.
Pico488 是一种染料，用于在存在 RNA、ssDNA 和其他杂质的情况下对 dsDNA 进行基于荧光的精确定量。 该染料在荧光素通道中显示出明亮的荧光，在低 pg/mL 至高 ng/mL 范围内对 DNA 浓度提供线性响应。

General properties

Appearance: dark green powder
Molecular weight: 844.05
CAS number: 1603861-95-5 (potassium salt); 1604244-45-2 (inner salt); 477908-53-5 (N-Ethyl)
Molecular formula: C41H46N3KO10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04

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  2. Trac, N.; Chen, L.-Y.; Zhang, A.; Liao, C.-P.; Poon, C.; Wang, J.; Ando, Y.; Joo, J.; Garri, C.; Shen, K.; Kani, K.; Gross, M.E.; Chung, E.J. CCR2-targeted micelles for anti-cancer peptide delivery and immune stimulation. Journal of Controlled Release, 2021, 329, 614–623. doi: 10.1016/j.jconrel.2020.09.054
  3. González, L.F.; Acuña, E.; Arellano, G.; Morales, P.; Sotomayor, P.; Oyarzun-Ampuero, F.; Naves, R. Intranasal delivery of interferon-β-loaded nanoparticles induces control of neuroinflammation in a preclinical model of multiple sclerosis: A promising simple, effective, non-invasive, and low-cost therapy. Journal of Controlled Release, 2021, 331, 443–459. doi: 10.1016/j.jconrel.2020.11.019
  4. Shapoval, O.; Oleksa, V.; Šlouf, M.; Lobaz, V.; Trhlíková, O.; Filipová, M.; Janoušková, O.; Engstová, H.; Pankrác, J.; Modrý, A.; Herynek, V.; Ježek, P.; Šefc, L.; Horák, D. Colloidally Stable P(DMA-AGME)-Ale-Coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ Nanoparticles as a Multimodal Contrast Agent for Down- and Upconversion Luminescence, Magnetic. Nanomaterials, 2021, 11(1), 230. doi: 10.3390/nano11010230
Cy® is a trademark of GE Healthcare.

Sulfo-Cy5.5 NHS ester

Sulfo-Cy5.5 NHS ester,Sulfo-Cy5.5 NHS 酯是磺化远红 Cy5.5 荧光团（Cy5.5® 的类似物）的胺反应活性酯。 这是标记抗体、敏感蛋白质和其他需要在纯水环境中反应或无需大量添加有机助溶剂的试剂的首选试剂。

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  • Reactive dyes
  • Dye NHS esters
  • Sulfo-Cyanine5.5 NHS ester

Sulfo-Cy5.5 NHS ester

标签：NHS esters Near infrared (NIR) imaging Microscopy Protein labeling sulfo-Cy5.5
货号 规格 价格 货期
17320 1 mg 110.00$ in stock
27320 5 mg 290.00$ in stock
47320 25 mg 690.00$ in stock
57320 50 mg 1190.00$ 5 days
67320 100 mg 1850.00$ in stock

Sulfo-Cyanine5.5 NHS ester is an amine reactive activated ester of sulfonated far red Cyanine5.5 fluorophore, an analog of Cy5.5®. This is a reagent of choice for the labeling of antibodies, sensitive proteins, and others which require reactions in purely aqueous environment, or without any significant additions of organic co-solvents.

The dye is well suitable for non-invasive in vivo NIR imaging, and for the applications requiring low fluorescent background.

Sulfo-Cy5.5 NHS 酯是磺化远红 Cy5.5 荧光团（Cy5.5® 的类似物）的胺反应活性酯。 这是标记抗体、敏感蛋白质和其他需要在纯水环境中反应或无需大量添加有机助溶剂的试剂的首选试剂。

该染料非常适用于非侵入性体内近红外成像，以及需要低荧光背景的应用。
Sulfo-Cyanine5.5 NHS ester Sulfo-Cyanine5.5 NHS ester Sulfo-Cyanine5.5 NHS ester Sulfo-Cyanine5.5 NHS ester

Absorption and emission spectra of sulfo-Cyanine5.5 fluorophore

Sulfo-Cyanine5.5 NHS ester

Sulfo-Cy5.5 NHS ester相关产品

Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 is a water-soluble Cyanine5 dye alkyne for Click chemistry.
Sulfo-Cyanine5 是一种用于 Click 化学的水溶性 Cyanine5 染料炔烃。

Cyanine7 azide

Near-infrared fluorescent dye for Click Chemistry.
用于点击化学的近红外荧光染料。

General properties

Appearance: dark blue solid
Mass spec M+ increment: 884.15
Molecular weight: 1114.37
CAS number: 2419286-92-1
Molecular formula: C44H42N3K3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 675
ε, L⋅mol−1⋅cm−1: 211000
Emission maximum, nm: 694
Fluorescence quantum yield: 0.21
CF260: 0.09
CF280: 0.11

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  2. Lu, Z.; Zhang, Y.; Wang, Y.; Tan, G.-H.; Huang, F.-Y.; Cao, R.; He, N.; Zhang, L. A biotin-avidin-system-based virus-mimicking nanovaccine for tumor immunotherapy. Journal of Controlled Release, 2021, 332, 245–259. doi: 10.1016/j.jconrel.2021.02.029
  3. Shramova, E.; Proshkina, G.; Shipunova, V.; Ryabova, A.; Kamyshinsky, R.; Konevega, A.; Schulga, A.; Konovalova, E.; Telegin, G.; Deyev, S. Dual Targeting of Cancer Cells with DARPin-Based Toxins for Overcoming Tumor Escape. Cancers, 2020, 12(10), 3014. doi: 10.3390/cancers12103014
  4. Peplau, E.; De Rose, F.; Reder, S.; Mittelhaeuser, M.; Scafetta, G.; Schwaiger, M.; Weber, W.A.; Bartolazzi, A.; Skerra, A.; D’Alessandria, C. Development of a chimeric Fab directed against human galectin-3 and validation as an immune-PET tracer for the sensitive in vivo imaging of thyroid cancer. Thyroid, 2020, 30(9), 1314–1326. doi: 10.1089/thy.2019.0670
Cy® is a trademark of GE Healthcare.

Cyanine3.5 NHS ester

Cyanine3.5 NHS ester，Cyanine3.5 NHS 酯（Cy3.5® NHS 酯的类似物）是一种活性染料，用于标记肽、蛋白质和寡核苷酸中的氨基。Cyanine3.5 NHS 酯可替代 Cy3.5®、DyLight 594 的 NHS 酯。

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  • Cyanine3.5 NHS ester

Cyanine3.5 NHS ester

标签：Cyanine3.5 NHS esters Protein labeling
货号 规格 价格 货期
12020 1 mg 110.00$ in stock
22020 5 mg 210.00$ in stock
42020 25 mg 410.00$ 5 days
52020 50 mg 695.00$ in stock
62020 100 mg 1190.00$ in stock

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, DyLight 594.

Cyanine3.5 NHS 酯（Cy3.5® NHS 酯的类似物）是一种活性染料，用于标记肽、蛋白质和寡核苷酸中的氨基。

Cyanine3.5 NHS 酯可替代 Cy3.5®、DyLight 594 的 NHS 酯。

Cyanine3.5 NHS ester Cyanine3.5 NHS ester Cyanine3.5 NHS ester Cyanine3.5 NHS ester

Cyanine3.5 NHS ester absorbance and emission spectra

Cyanine3.5 NHS ester

Cyanine3.5 NHS ester相关产品

Sulfo-Cyanine5.5 maleimide

Sulfo-Cyanine5.5 is a water-soluble far red emitting dye. This is a thiol reactive maleimide derivative of sulfo-Cyanine5.5. It is recommended for the labeling of sensitive proteins.

Sulfo-Cyanine5.5 是一种水溶性远红发射染料。 这是 sulfo-Cyanine5.5 的硫醇反应性马来酰亚胺衍生物。 推荐用于敏感蛋白的标记。

Cyanine7.5 amine

Near infrared dye Cyanine7.5 with free amino group.

具有游离氨基的近红外染料Cyanine7.5。

ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

ROX（又名罗丹明 X 或罗丹明 101）叠氮化物，用于通过 Click Chemistry 进行生物分子标记。

General properties

Appearance: dark purple solid
Molecular weight: 741.62
CAS number: 2231670-85-0
Molecular formula: C42H44N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22

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