标签： ProSpec-TNFSF14 Mouse

Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light.

TNFRSF14, a member of the TNF receptor superfamily, is a type I transmembrane protein. TNFRSF14 is expressed in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. TNFRSF14 operates as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. Additionally, TNFRSF14 encourages the proliferation of T cells, and triggers apoptosis of various tumor cells.

TNFRSF14 是 TNF 受体超家族的成员，是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。 TNFRSF14 作为激活淋巴样细胞的共刺激因子和对疱疹病毒感染的威慑物起作用。 此外，TNFRSF14 促进 T 细胞的增殖，并引发各种肿瘤细胞的凋亡。

TNFSF14 Mouse Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 168 amino acids and having a molecular mass of 18.4kDa.

在大肠杆菌中产生的 TNFSF14 Mouse Recombinant 是一种单一的、非糖基化的多肽链，含有 168 个氨基酸，分子量为 18.4kDa。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

TNFSF14 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS pH7.4.

It is recommended to reconstitute the lyophilized TNFSF14 in sterile 100mM HAc not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 TNFSF14 溶解在不低于 100µg/ml 的无菌 100mM HAc 中，然后可以进一步稀释成其他水溶液。

Lyophilized TNFSF14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFSF14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFSF14 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后 TNFSF14 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Analysis by -PAGE.

DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV.

The ED₅₀ as determined by a cytotoxicity assay using human HT-29 cells is less than 2µg/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-g.

通过使用人 HT-29 细胞的细胞毒性测定法确定的 ED50 小于 2μg/ml，对应于在鼠抗多组氨酸单克隆抗体和 rHuIFN-g 存在下 > 500 IU/mg 的比活性。

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