标签： ProSpec-TNFSF12 Human

TWEAK, TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3-Ligand, APO3L, TNFRSF12A, Tumor necrosis factor ligand superfamily member 12, MGC20669, MGC129581.

TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion. TNFSF12 exerts an apoptotic activity in HT-29 human adenocarcinoma cells whilst cultured in the presence of IFN-?. TNFSF12 promotes proliferation and migration of endothelial cells.

TNFSF12 是一种细胞因子，是 TNF 配体家族的一部分。 TNFSF12 是 FN14/TWEAKR 受体的配体。 TNFSF12 具有与 TNF 重叠的信号功能，但显示出更广泛的组织分布。 TNFSF12 通过多种细胞死亡途径以细胞类型特异性方式诱导细胞凋亡。 TNFSF12 促进内皮细胞的增殖和迁移，因此充当血管生成的调节剂。 TNFSF12 在成人心脏、胰腺、骨骼肌、小肠、脾脏和外周血淋巴细胞中表达。 TWEAK h 诱导 NFkB 和趋化因子分泌。 在存在 IFN-α 的情况下培养时，TNFSF12 在 HT-29 人腺癌细胞中发挥凋亡活性。 TNFSF12 促进内皮细胞的增殖和迁移。

TNFSF12 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a total molecular mass of 18kDa.

The TNFSF12 is fused with an 8 amino acids his tag at N-terminal and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFSF12 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 156 个氨基酸，总分子量为 18kDa。

TNFSF12 在 N 端融合了 8 个氨基酸的 his 标签，并通过专有色谱技术进行纯化。

Escherichia coli.

Filtered White lyophilized powder.

Lyophilized from a 0.2μm filtered solution in PBS.

It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TNFSF12，然后可以进一步稀释成其他水溶液。

Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein .

冻干的 TNFSF12 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TWEAK 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

对于长期储存，建议添加载体蛋白。

Please prevent freeze-thaw cycles.

MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH.

Greater than 95% as determined by -PAGE.

The ED₅₀, as measured in a proliferation assay using HUVECs, is less than 8ng/ml.

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