标签： ProSpec-RELM a Mouse His

Resistin-like alpha, RELMalpha, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-gamma, RELM-a.

Bronchoalveolar lavage fluid from mice with experimentally induced allergic pulmonary inflammation contains a novel 9.4 kDa cysteine-rich secreted protein, RELM-alpha . RELM-alpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue stroma. Murine RELM-alpha is the founding member of a new gene family including two other murine genes expressed, respectively, in intestinal crypt epithelium and white adipose tissue , and two related human genes.
RELMalpha inhibits the differentiation of 3T3-L1 preadipocytes into adipocytes but has no effect on proliferation of 3T3-L1 preadipocytes. RELMalpha is able to form heterooligomers with resistin but not RELMbeta. Since RELMalpha is expressed by adipose tissue and it is a secreted factor, our findings suggest that RELMalpha may be involved in the control of the adipogenesis as well as in the process of muscle differentiation.
In the lung, RELM-alpha is induced by hypoxia and was renamed as hypoxia-induced mitogenic factor . HIMF strongly activated Akt phosphorylation. The phosphatidylinositol 3-kinase inhibitor LY294002 inhibited HIMF-activated Akt phosphorylation. It also inhibited HIMFstimulated RPSM proliferation. Thus, the PI3K/Akt pathway, at least in part, mediates the proliferative effect of HIMF. Further studies showed that HIMF had angiogenic and vasoconstrictive properties. HIMF increased pulmonary arterial pressure and vascular resistance. Further studies suggest that HIMF regulates apoptosis and may participate in lung alveolarization and maturation.

来自患有实验性过敏性肺部炎症的小鼠的支气管肺泡灌洗液含有一种新型的 9.4 kDa 富含半胱氨酸的分泌蛋白 RELM-α。 RELM-α 是一种分泌蛋白，其组织分布受限，在脂肪组织基质中含量最高。鼠类 RELM-α 是一个新基因家族的创始成员，该家族包括另外两个分别在肠隐窝上皮和白色脂肪组织中表达的鼠类基因，以及两个相关的人类基因。
RELMalpha 抑制 3T3-L1 前脂肪细胞分化为脂肪细胞，但对 3T3-L1 前脂肪细胞的增殖没有影响。 RELMalpha 能够与抵抗素形成异源寡聚体，但不能与 RELMbeta 形成。由于 RELMalpha 由脂肪组织表达并且它是一种分泌因子，我们的研究结果表明 RELMalpha 可能参与控制脂肪生成以及肌肉分化过程。
在肺中，RELM-α是由缺氧诱导的，并被重新命名为缺氧诱导的有丝分裂因子。 HIMF 强烈激活 Akt 磷酸化。磷脂酰肌醇 3-激酶抑制剂 LY294002 抑制 HIMF 激活的 Akt 磷酸化。它还抑制 HIMF 刺激的 RPSM 增殖。因此，PI3K/Akt 通路至少部分介导了 HIMF 的增殖作用。进一步的研究表明，HIMF 具有血管生成和血管收缩特性。 HIMF 增加肺动脉压和血管阻力。进一步的研究表明，HIMF 调节细胞凋亡并可能参与肺泡化和成熟。

RELM-alpha Mouse Recombinant is manufactured with a signal sequence of phage fd and C-terminal fusion of flagTag . The RELM-alpha Flag-Tagged Fusion Protein is a 13.3 kDa protein containing 91 amino acid residues with 30 additional amino acid residues – signal sequence of phage fd, flagTag .

RELM-alpha Mouse Recombinant 是用噬菌体 fd 的信号序列和 flagTag 的 C 端融合物制造的。 RELM-alpha Flag-Tagged 融合蛋白是一种 13.3 kDa 的蛋白质，含有 91 个氨基酸残基和 30 个额外的氨基酸残基——噬菌体 fd 的信号序列 flagTag 。

Escherichia Coli.

Filtered White lyophilized powder.

Filtered and lyophilized from 0.5 mg/ml in 5mM Tris pH 7.5, 25mM NaCl.

Add deionized H2O and let the lyophilized pellet dissolve completely.

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

MKKLLFAIPL VVPFYSHSTM VNTDETIEII VENKVKELLA NPANYPSTVT TLSCTSVKT MNRWASCPAG MTATGCACGF ACGSWEIQSG DTCNCLCLLV DWTTARCCQL SLEDYKDDDD K.

Greater than 95% as determined by -PAGE.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.