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MCP 3 Rat
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Monocyte Chemotactic Protein-3 Rat Recombinant
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CHM-280
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Catalogue number
CHM-280
Synonyms
C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, MCP-3, Small-inducible cytokine A7, Ccl7, Mcp3, Scya7.
Introduction
Chemokine ligand 7 is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 . Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature protein, it is classified among the subfamily of chemokines known as CC chemokines. CCL7 specifically attracts monocytes, and regulates macrophage function. It is produced by certain tumor cell lines and by macrophages. This chemokine is located on chromosome 17 in humans, in a large cluster containing many other CC chemokines and is most closely related to CCL2.
趋化因子配体 7 是一种称为趋化因子的小细胞因子,以前称为单核细胞特异性趋化因子 3。 由于 CCL7 在其成熟蛋白中具有两个相邻的 N 末端半胱氨酸残基,因此它被归类为称为 CC 趋化因子的趋化因子亚家族。 CCL7 特异性吸引单核细胞,并调节巨噬细胞功能。 它由某些肿瘤细胞系和巨噬细胞产生。 这种趋化因子位于人类的第 17 号染色体上,位于一个包含许多其他 CC 趋化因子的大簇中,并且与 CCL2 关系最密切。
Description
MCP-3 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8.5kDa.
The MCP-3 is purified by proprietary chromatographic techniques.
The MCP-3 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
MCP-3 protein was lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, 300mM NaCl, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized MCP-3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized MCP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCP3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
Please prevent freeze-thaw cycles.
冻干的 MCP-3 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 复溶后,MCP3 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。
请防止冻融循环。
Purity
Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.
Analysis by RP-HPLC.
Analysis by -PAGE.
Amino acid sequence
QPDGTNSSTC CYVKKQKIPK RNLKSYRKIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTS TPKP.
Biological Activity
Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50.0-300.0 ng/ml.
Usage
Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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