标签： ProSpec-IHH Human

ProSpec-IHH Human/CYT-195

ProSpec-IHH Human,ProSpec CYT-195,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,IHH 属于分泌信号分子的刺猬家族。 Hedgehog 蛋白是各种发育过程的重要调节剂，包括生长、模式和形态发生。 Hh 的脊椎动物同源物包括几种蛋白质，包括声波刺猬、印度刺猬和沙漠刺猬。

IHH Human

Indian Hedgehog Human Recombinant
CYT-195
ProSpec-IHH Human/CYT-195 5µg
ProSpec-IHH Human/CYT-195 25µg
ProSpec-IHH Human/CYT-195 1mg

Catalogue number

CYT-195

Synonyms

Indian hedgehog protein, IHH, HHG-2, BDA1.

Introduction

IHH belongs to the hedgehog family of secreted signaling molecules. Hedgehog proteins are vital regulators of various developmental processes including growth, patterning and morphogenesis. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog , Indian hedgehog , and Desert hedgehog . IHH has a specific role in bone growth and differentiation. In addition, IHH is involved in yolk sac vasculogenesis, having a central role in differentiation of epiblast cells into endothelial and red blood cells. IHH mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas and strongly in cartilage in growth regions of the developing bone. IHH gene mutations cause the brachydactyly type A1 which is characterized by shortening or malformation of the phalanges and also the acrocapitofemoral dysplasia.
IHH 属于分泌信号分子的刺猬家族。 Hedgehog 蛋白是各种发育过程的重要调节剂，包括生长、模式和形态发生。 Hh 的脊椎动物同源物包括几种蛋白质，包括声波刺猬、印度刺猬和沙漠刺猬。 IHH 在骨骼生长和分化中具有特定的作用。 此外，IHH 参与卵黄囊血管发生，在外胚层细胞分化为内皮细胞和红细胞中起核心作用。 在胎儿肺、肠、胃、肝、肾、胰腺中检测到 IHH mRNA 表达，并且在发育中骨骼的生长区域的软骨中强烈检测到。 IHH 基因突变导致 A1 型短指，其特征是指骨缩短或畸形以及顶股骨发育不良。

Description

IHH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8kDa.
The IHH is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 IHH Human Recombinant 是一条单一的、非糖基化的多肽链，包含 176 个氨基酸，分子量为 19.8kDa。
IHH 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized IHH in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized IHH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IHH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Amino acid sequence

IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG.

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Biological Activity

Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 cells. The expected ED50 for this effect is 3.0-10.0?g/ml corresponding to a specific activity of 100-334units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-SHH Human, His/CYT-717

ProSpec-SHH Human His,ProSpec CYT-717,ProSpec SHH Mouse,ProSpec IHH Human,在大肠杆菌中产生的 Sonic HedgeHog Recombinant Human 是一条单一的、非糖基化的多肽链，含有 183 个氨基酸，分子量为 20.7kDa。 Sonic HedgeHog 在 C 端与 8 个氨基酸 His-Tag 融合，并通过专有色谱技术进行纯化。

SHH Human, His

Sonic HedgeHog Human Recombinant, His Tag
CYT-717
ProSpec-SHH Human, His/CYT-717 5µg
ProSpec-SHH Human, His/CYT-717 25µg
ProSpec-SHH Human, His/CYT-717 1mg

Catalogue number

CYT-717

Synonyms

SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.

Introduction

Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the
重组人声波刺猬是一小部分分泌蛋白的一部分，这些蛋白对脊椎动物和无脊椎动物的发育都至关重要。 3个哺乳动物刺猬基因具有约60%的同源性。 人类声波刺猬与小鼠基因有 99% 的同源性。 Sonic HedgeHog 是一种对引导早期胚胎至关重要的蛋白质。 它被认为是腹侧神经管、前后肢轴和腹侧体节的主要诱导信号。 Sonic HedgeHog 与修补受体结合，该受体与 Smoothened 相关联，以激活靶基因的转录。 在没有声波 HedgeHog 的情况下，修补受体抑制了平滑的本构信号活性。 Sonic HedgeHog 还调节另一个因素，即 gli 癌基因。 Sonic HedgeHog 细胞间信号对于发育过程中的各种模式事件至关重要：脊索产生的信号可诱导腹侧细胞命运

Description

Sonic HedgeHog Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 183 amino acids and having a molecular mass of 20.7kDa. The Sonic HedgeHog is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Sonic HedgeHog Recombinant Human 是一条单一的、非糖基化的多肽链，含有 183 个氨基酸，分子量为 20.7kDa。 Sonic HedgeHog 在 C 端与 8 个氨基酸 His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

SHH Human solution containing 20mM Tris HCL pH-8, 0.1M NaCl, & 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein . Please prevent freeze thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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