标签： ProSpec-IFN a 2a Human

Leukocyte IFN, B cell IFN, Type I IFN, IFNA2, IFN-a 2a, Interferon-alpha-2a.

IFN-alpha is produced by macrophages and has antiviral activities. IFN stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.

IFN-α 由巨噬细胞产生并具有抗病毒活性。 干扰素刺激两种酶的产生：蛋白激酶和寡腺苷酸合成酶。

IFN Alpha Human 2a Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine.
The Interferon-alpha-2a gene was obtained from human leukocytes.
The IFNA2A is purified by proprietary chromatographic techniques.

IFN-α 由巨噬细胞产生并具有抗病毒活性。 干扰素刺激两种酶的产生：蛋白激酶和寡腺苷酸合成酶。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized without additives.

It is recommended to reconstitute the lyophilized Interferon-alpha-2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized IFNA-2A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 IFNA-2A 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，IFN-α 2a 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by both:
Analysis by RP-HPLC.
Analysis by -PAGE.

CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEM IQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAV RKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE.

N-terminal methionine has been completely removed enzymatically.

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 270,000,000 IU/mg.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of IFN-a 2a as a Reference Standard.

蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 0.924 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. 通过 RP-HPLC 分析，使用 IFN-a 2a 的校准溶液作为参考标准。

1.Title:Gender specificity of altered human immune cytokine profiles in aging.
Publication:Published online before print May 7, 2010, doi: 10.1096/fj.10-160911 September 2010 The FASEB Journal vol. 24 no. 9 3580-3589
Link:http://www.fasebj.org/content/24/9/3580.full

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