标签： ProSpec GDF5 Mouse

Bmp-14, Bp, GDF-5, Bone morphogenetic protein 14, GDF5, Growth/differentiation factor 5.

GDF-5 is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.

GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。 该家族的成员是胚胎和成人组织中细胞生长和分化的调节剂。 该基因的突变与Hunter-Thompson型肢端发育不良有关； 短指，C型； 和软骨发育不良，Grebe型。 这些关联证实基因产物在骨骼发育中发挥作用。

GDF5 Mouse Recombinant produced in E.coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 120 amino acids and having a molecular mass of 27.2kDa.
The GDF-5 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 GDF5 Mouse Recombinant 是一种非糖基化的二硫键连接的同源二聚体，包含 2 条 120 个氨基酸的链，分子量为 27.2kDa。
GDF-5 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

It is recommended to reconstitute the lyophilized GDF5 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 4mM HCl 中复溶冻干的 GDF5，然后可以进一步稀释成其他水溶液。

Lyophilized GDF5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF-5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 GDF5 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，GDF-5 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0µg/ml, corresponding to a specific activity of > 1000IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。