标签： ProSpec-FST Human

FST, FS

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin  functions as an ACTV antagonist. specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone .

卵泡抑素是一种单链性腺蛋白，可特异性抑制促卵泡激素的释放。 单个 FST 基因编码两种同种型，FST317 和 FST344，分别含有 317 和 344 个氨基酸，由前体 mRNA 的可变剪接产生。 在一项研究中，测试了 37 个候选基因与 150 个家庭的多囊卵巢综合征或高雄激素血症的关联和关联，发现了 PCOS 和卵泡抑素之间关联的证据。 卵泡抑素起到 ACTV 拮抗剂的作用。 垂体促卵泡激素的生物合成和分泌的特异性抑制剂。

Follistatin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa.
The FST is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Follistatin Human Recombinant 是一条单一的、非糖基化的多肽链，包含 288 个氨基酸，总分子量为 31.5kDa。
FST 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a concentrated solution containing no additives.

It is recommended to reconstitute the lyophilized Follistatin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Follistatin，然后可以进一步稀释成其他水溶液。

Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FST should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 Follistatin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，FST 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Asn-Cys-Trp-Leu.

The activity is determined by the ability to neutralize ACTV inhibitory effect of mouse MPC-11 cells. The expected ED50 is 100-400ng/ml, corresponding to a Specific Activity of 2,500-10,000units/mg in the presence of 7.5ng/ml ACTV A.

活性取决于中和小鼠 MPC-11 细胞的 ACTV 抑制作用的能力。 预期的 ED50 为 100-400ng/ml，对应于 7.5ng/ml ACTV A 的比活性为 2,500-10,000units/mg。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.