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Fractalkine Human
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Fractalkine Human Recombinant
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CHM-235
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Catalogue number
CHM-235
Synonyms
Fractalkine, CX3CL1, Neurotactin, CX3C membrane-anchored chemokine, Small inducible cytokine D1, NTN, NTT, CXC3, CXC3C, SCYD1, ABCD-3, C3Xkine.
Introduction
Fractalkine 可溶形式对 t 细胞和单核细胞具有趋化性，但对中性粒细胞不具有趋化性。 Fractalkine 膜结合形式促进这些白细胞与内皮细胞的粘附。 Fractalkine 调节内皮的白细胞粘附和迁移过程并与 CX3CR1 结合。 Natural Human Fractalkine 是一种长蛋白，具有延伸的粘蛋白样茎和顶部的趋化因子结构域。 粘蛋白样茎允许它与细胞表面结合。 Fractalkine 基因与一些称为 CCL17 和 CCL22 的 CC 趋化因子一起位于人类第 16 号染色体上。
Description
Fractalkine Human Recombinant- produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8638 Dalton.
The Fractalkine is purified by proprietary chromatographic techniques.
The Fractalkine is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The CX3CL1 was lyophilized from a 0.2μm filtered concentrated solution in 20mM Phosphate buffer, pH 7.4, 50mM NaCl.
Solubility
It is recommended to reconstitute the lyophilized CX3CL1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized CX3CL1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CX3CL1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.
Analysis by RP-HPLC.
Analysis by -PAGE.
Amino acid sequence
QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQW VKDAMQHLDRQAAALTRNG.
Biological Activity
The Biological activity is calculated by its ability to chemoattract human T-Lymphocytes using a concentration range of 5.0-10.0 ng/ml corresponding to a Specific Activity of 100,000-200,000IU/mg.
References
Title: Glial Cell Line-Derived Neurotrophic Factor and Neurturin Inhibit Neurite Outgrowth and Activate RhoA through GFR?2b, an Alternatively Spliced Isoform of GFR?2.
Publication: The Journal of Neuroscience, 23 May 2007, 27: 5603-5614; doi: 10.1523/?JNEUROSCI.4552-06.2007.
Link: http://www.jneurosci.org/content/27/21/5603.full
Applications: GFR?2 when activated by NTN, it inhibits neurite outgrowth induced by GFR?1a, GFR?2a, and GFR?2c isoforms.
Publication: The Journal of Neuroscience, 23 May 2007, 27: 5603-5614; doi: 10.1523/?JNEUROSCI.4552-06.2007.
Link: http://www.jneurosci.org/content/27/21/5603.full
Applications: GFR?2 when activated by NTN, it inhibits neurite outgrowth induced by GFR?1a, GFR?2a, and GFR?2c isoforms.
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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