Catalogue number
CYT-706
Synonyms
Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
Introduction
FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
FLT3 配体是 fl 细胞因子的受体,具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。
Description
Flt3-Ligand Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 24-30kDa due to glycosylation. The Flt3-Ligand is purified by proprietary chromatographic techniques.
在 HEK 细胞中产生的 Flt3-Ligand Human Recombinant 是一种糖基化单体,由于糖基化,其分子量范围为 24-30kDa。 Flt3-Ligand 通过专有的色谱技术进行纯化。
Source
HEK293 .
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Flt3-Ligand was lyophilized from a 0.2µm filtered solution containing 1xPBS.
Solubility
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Flt3-Ligand,然后可以进一步稀释成其他水溶液。
Stability
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.
冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 复溶后,Flt3-Ligand 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。 请防止冻融循环。
Purity
Greater than 95.0% as determined by: Analysis by RP-HPLC. Analysis by -PAGE.
Amino acid sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP.
Biological Activity
The activity was determined by the dose- dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5. The ED50 is 0.56ng/ml.
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.