标签： ProSpec-DHH Human

SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.

Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.

重组人声波刺猬是一小部分分泌蛋白的一部分，这些蛋白对脊椎动物和无脊椎动物的发育都至关重要。 3个哺乳动物刺猬基因具有约60%的同源性。人类声波刺猬与小鼠基因有 99% 的同源性。 Sonic HedgeHog 是一种对引导早期胚胎至关重要的蛋白质。它被认为是腹侧神经管、前后肢轴和腹侧体节的主要诱导信号。 Sonic HedgeHog 与修补受体结合，该受体与 Smoothened 相关联，以激活靶基因的转录。在没有声波 HedgeHog 的情况下，修补受体抑制了平滑的本构信号活性。 Sonic HedgeHog 还调节另一个因素，即 gli 癌基因。 Sonic HedgeHog 细胞间信号对于发育过程中的各种模式事件至关重要：由脊索产生的信号，该信号诱导神经管和体节中的腹侧细胞命运，以及用于形成发育中的肢芽前后轴模式的极化信号。 Sonic HedgeHog 表现出底板和运动神经元诱导活动。位于肢体区域 1 基因内含子的远程 Sonic HedgeHog 增强子的突变导致轴前多指畸形。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

SHH is lyophilized from 10mM Na3PO4, pH 7.5.

It is recommended to reconstitute the lyophilized SHH in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Human Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

冻干的人声刺猬虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，Sonic HedgeHog 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Greater than 95.0% as determined by -PAGE.

MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGGCFP

The ED50 is measured by the dose-dependent induction of alkaline phosphatase production by CCL-226 fibroblasts and is 1.47μg/ml corresponding to a specific activity of 680U/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.