ProSpec-TNF a Rat, His/CYT-900,Tumor Necrosis Factor-alpha Rat Recombinant, His Tag

ProSpec-TNF a Rat, His/CYT-900,Tumor Necrosis Factor-alpha Rat Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor Necrosis Factor-alpha, TNF a His, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.

TNF a Rat, His
Tumor Necrosis Factor-alpha Rat Recombinant, His Tag
CYT-900
5µg
20µg
1mg

Catalogue number

CYT-900

Synonyms

Tumor Necrosis Factor-alpha, TNF a His, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子,是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制,TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调,特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNF a Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids and having a molecular mass of 19.9kDa.
TNF a is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNF a Rat Recombinant 是一种单一的、非糖基化的多肽链,含有 181 个氨基酸,分子量为 19.9kDa。
TNF a 在 N 末端与 25 个氨基酸的 His-tag 融合,并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNF a protein solution containing Phosphate Buffer Saline , 10% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by analysis by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF a Rhesus Macaque/CYT-737,Tumor Necrosis Factor-Alpha Rhesus Macaque Recombinant

ProSpec-TNF a Rhesus Macaque/CYT-737,Tumor Necrosis Factor-Alpha Rhesus Macaque Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 5% trehalose.

TNF a Rhesus Macaque
Tumor Necrosis Factor-Alpha Rhesus Macaque Recombinant
CYT-737
5µg
20µg
1mg

Catalogue number

CYT-737

Synonyms

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子,是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制,TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调,特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNF-a Rhesus Macaque Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17.3kDa.
The TNFA Rhesus Macaque is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNF-a Rhesus Macaque Recombinant 是一种单一的、非糖基化的多肽链,包含 157 个氨基酸,分子量为 17.3kDa。
TNFA Rhesus Macaque 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 5% trehalose.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子-a 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后的 TNF-a 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL.

Biological Activity

The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF alpha human/CYT-223,Tumor Necrosis Factor-Alpha Human Recombinant

ProSpec-TNF alpha human/CYT-223,Tumor Necrosis Factor-Alpha Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,肿瘤坏死因子是一种参与全身炎症的细胞因子,是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。

TNF alpha human
Tumor Necrosis Factor-Alpha Human Recombinant
CYT-223
10µg
50µg
1mg

Catalogue number

CYT-223

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, INS resistance, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子,是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制,TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调,特别是 TNF 的过度产生与多种人类疾病有关——自身免疫性疾病、INS 抗性和癌症。

Description

Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.5kDa.
The TNF-alpha is purified by standard chromatographic techniques.
在大肠杆菌中产生的肿瘤坏死因子-a 人重组体是一种单一的、非糖基化的多肽链,含有 158 个氨基酸,分子量为 17.5kDa。
TNF-α通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNF-a Human was lyophilized from a concentrated 1mg/ml solution containing 20mM PB, pH-7.2, and 100mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的肿瘤坏死因子-α,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的肿瘤坏死因子-a 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后的 TNF-a 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.

Biological Activity

The Specific Activity is >5.0×107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Background

Tnf-alpha stands for tumour necrosis factor, and it is a cell signalling protein found in the human body. Part of systemic inflammation, it is just one of the cytokines that trigger the acute phase reaction. These proteins with plasma concentrations decrease or increase in response to inflammation.

Tnf-alpha代表肿瘤坏死因子,是一种存在于人体内的细胞信号蛋白。 全身炎症的一部分,它只是引发急性期反应的细胞因子之一。 这些具有血浆浓度的蛋白质响应炎症而降低或增加。

Mechanisms
Tnf-alpha is typically produced by macrophages being activated. A type of white blood cell, these calls digest cellular debris and cancer cells or really any type of protein that doesn’t have surface healthy body cells. However, it is possible that it can be produced through other cell types includes NK cells, eosinophils, neurons, lymphocytes as well as various others.

机制
Tnf-alpha 通常由巨噬细胞被激活产生。 一种白细胞,这些调用消化细胞碎片和癌细胞,或者实际上是任何没有表面健康体细胞的蛋白质。 然而,它有可能通过其他细胞类型产生,包括 NK 细胞、嗜酸性粒细胞、神经元、淋巴细胞以及各种其他细胞。

 

Function
The main function of Tnf-alpha is to regulate immune cells in the body. Because it is endogenous pyrogen, the protein is able to induce everything from inflammation to apoptotic cell death. Furthermore, the protein can also prevent tumorigenesis and respond to sepsis. It does this through IL1 and IL6 producing cells. Various human diseases have been associated with or connected to the dysregulation of Tnf production. Some of these diseases include, Alzheimer’s, cancer, depression and IBD. Furthermore, Tnf can be created ectopically, in the presence of a malignant parathyroid hormone, particularly in cancers where there is typically an increased level of production.

Tnf-alpha的主要功能是调节体内的免疫细胞。 因为它是内源性热原,蛋白质能够诱导从炎症到细胞凋亡的一切。 此外,该蛋白质还可以预防肿瘤发生并对败血症作出反应。 它通过产生 IL1 和 IL6 的细胞来做到这一点。 多种人类疾病与肿瘤坏死因子产生的失调有关或相关。 其中一些疾病包括阿尔茨海默氏症、癌症、抑郁症和 IBD。 此外,在存在恶性甲状旁腺激素的情况下,可以异位产生 Tnf,特别是在通常产生水平增加的癌症中。

Interaction
TNF has various actions on different organ systems. For instance, on the liver, it stimulates the acute phase response and this leads to additional development of C-reactive protein as well as other mediators. Furthermore, it also provides INS resistance and it does this by triggering serine-phosphorylation of IRS-1. This negatively impacts INS signalling.
In the hypothalamus, TNF-alpha has been shown to both suppress appetite and trigger a fever.
Tnf-alpha has been studied and shown to interact with TNFRSF1A. This is a ubiquitous membrane receptor that can bind tumor necrosis factor alpha. This can also function as a regulator of levels of inflammation.
Studies have also shown interactions between IL-5 and Tnf-a genotypes that are associated with bacteremia present in patients with myeloma. The transplantation of stem cells impacts patients level of risk to infections due to changes to their immune system. Increased levels of TNF-a impact the immune response and an interaction analysis has shown that there was an interaction between the two. Further research has also shown that interactions between IL-10 and TNF-A are able to regulate prostaglandin E2 production and lipopolysaccharide-stimulated human choriodecidual cytokine.

TNF对不同的器官系统有不同的作用。例如,在肝脏上,它会刺激急性期反应,这会导致 C 反应蛋白和其他介质的额外发展。此外,它还提供 INS 抗性,它通过触发 IRS-1 的丝氨酸磷酸化来实现这一点。这会对 INS 信令产生负面影响。
在下丘脑中,TNF-α 已被证明可以抑制食欲并引发发烧。
TNF-alpha 已被研究并显示与 TNFRSF1A 相互作用。这是一种普遍存在的膜受体,可以结合肿瘤坏死因子α。这也可以作为炎症水平的调节剂。
研究还表明,IL-5 和 Tnf-a 基因型之间的相互作用与骨髓瘤患者的菌血症有关。由于免疫系统的变化,干细胞移植会影响患者感染风险的水平。增加的 TNF-a 水平会影响免疫反应,相互作用分析表明两者之间存在相互作用。进一步的研究还表明,IL-10 和 TNF-A 之间的相互作用能够调节前列腺素 E2 的产生和脂多糖刺激的人绒毛膜细胞因子。

 

Structure
TNF is primarily produced as a 233-amino acid long type II transmembrane protein. It is arranged in stable homotetramers. From this, the homotrimeric cytokine is released by the TNF alpha converting enzyme. This tends to disassociate at certain concentrations, usually those below the nanomolar range, making them lose their bioactivity. The human form of TNF-alpha is a triangle shape and is roughly 17-kd in weight.
Research is ongoing exploring the full impact of TNF-alpha on the human body including its effect on various psychological and physical conditions. Thus far, anti-TNF therapy has provided only a low impact as a form of cancer therapy.

TNF 主要作为 233 个氨基酸长的 II 型跨膜蛋白产生。 它以稳定的同源四聚体排列。 由此,同源三聚体细胞因子被 TNF α 转化酶释放。 这往往会在某些浓度下解离,通常是那些低于纳摩尔范围的浓度,使它们失去生物活性。 人类形式的 TNF-α 呈三角形,重约 17 kd。
正在进行研究以探索 TNF-α 对人体的全面影响,包括其对各种心理和身体状况的影响。 到目前为止,抗 TNF 疗法作为癌症疗法的一种形式仅提供了很小的影响。

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ProSpec-TNF b Human/CYT-224,Tumor Necrosis Factor-Beta Human Recombinant

ProSpec-TNF b Human/CYT-224,Tumor Necrosis Factor-Beta Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,冻干的肿瘤坏死因子-b 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后 TNF-b 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。对于长期储存,建议添加载体蛋白。请防止冻融循环。

TNF b Human
Tumor Necrosis Factor-Beta Human Recombinant
CYT-224
5µg
20µg
1mg

Catalogue number

CYT-224

Synonyms

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

Introduction

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Lymphotoxin alpha 是肿瘤坏死因子家族的成员,是一种由淋巴细胞产生的细胞因子。 LTA 具有高度诱导性、分泌性,并以同源三聚体分子的形式存在。 LTA 与淋巴毒素-β 形成异源三聚体,将淋巴毒素-α 锚定到细胞表面。 LTA 介导多种炎症、免疫刺激和抗病毒反应。 LTA 还参与发育过程中次级淋巴器官的形成,并在细胞凋亡中发挥作用。

Description

Tumor Necrosis Factor-b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 18645 Dalton.
The TNF-b is purified by standard chromatographic techniques.
在大肠杆菌中产生的肿瘤坏死因子-b 人重组体是一种单一的、非糖基化的多肽链,含有 172 个氨基酸,分子量为 18645 道尔顿。
TNF-b通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized protein with no additives.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Tumor Necrosis Factor-b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子-b 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后 TNF-b 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAG.

Amino acid sequence

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

Biological Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of TNF-b as a Reference Standard.
蛋白质定量通过两种独立的方法进行:
1. 280 nm 的紫外光谱,使用 1.082 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. 通过 RP-HPLC 分析,使用 TNF-b 的校准溶液作为参考标准。

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF b Human, His/CYT-495,Tumor Necrosis Factor-Beta Human Recombinant, His Tag

ProSpec-TNF b Human, His/CYT-495,Tumor Necrosis Factor-Beta Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNF-b protein solution contains 20mM Tris-HCl buffer , 30% glycerol, 0.1M NaCl and 1mM DTT.

TNF b Human, His
Tumor Necrosis Factor-Beta Human Recombinant, His Tag
CYT-495
5µg
20µg
1mg

Catalogue number

CYT-495

Synonyms

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

Introduction

TNF-b is a potent multifunctional cytokine produced mainly by activated T and B lymphocytes. The protein has been shown to have a specific potent cytotoxic activity on tumor cells and a variety of other target cells as well as being a mediator of inflammation and immune function.
TNF-b 是一种有效的多功能细胞因子,主要由活化的 T 和 B 淋巴细胞产生。 该蛋白质已被证明对肿瘤细胞和多种其他靶细胞具有特定的有效细胞毒活性,并且是炎症和免疫功能的介质。

Description

TNF-b Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 192 amino acids and having a molecular mass of 20.9kDa.
TNF-b is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNF-b Human Recombinant 是一种单一的、非糖基化的多肽链,含有 192 个氨基酸,分子量为 20.9kDa。
TNF-b 在 N 端与 21 个氨基酸的 His-tag 融合,并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNF-b protein solution contains 20mM Tris-HCl buffer , 30% glycerol, 0.1M NaCl and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

Biological Activity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is <0.3 ng/ml.

在代谢抑制剂放线菌素 D 存在下使用 L929 小鼠纤维肉瘤细胞在细胞毒性测定中测量。该效应的 ED50 为 <0.3 ng/ml。

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TNF B Human, Sf9
  • LTA Antibody
  • TNF b Human

ProSpec-TNF B Human, Sf9/CYT-989,Tumor Necrosis Factor-beta Human Recombinant, Sf9

ProSpec-TNF B Human, Sf9/CYT-989,Tumor Necrosis Factor-beta Human Recombinant, Sf9,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNFB protein solution contains Phosphate Buffered Saline and 10% glycerol.

TNF B Human, Sf9
Tumor Necrosis Factor-beta Human Recombinant, Sf9
CYT-989
1µg
5µg
50µg

Catalogue number

CYT-989

Synonyms

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

Introduction

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Lymphotoxin alpha 是肿瘤坏死因子家族的成员,是一种由淋巴细胞产生的细胞因子。 LTA 具有高度诱导性、分泌性,并以同源三聚体分子的形式存在。 LTA 与淋巴毒素-β 形成异源三聚体,将淋巴毒素-α 锚定到细胞表面。 LTA 介导多种炎症、免疫刺激和抗病毒反应。 LTA 还参与发育过程中次级淋巴器官的形成,并在细胞凋亡中发挥作用。

Description

Tumor Necrosis Factor-beta Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 180 amino acids and having a molecular mass of 19.7kDa .
TNFB is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9杆状病毒细胞产生的Tumor Necrosis Factor-beta Human Recombinant是一条单一的糖基化多肽链,包含180个氨基酸,分子量为19.7kDa。
TNFB 在 C 末端与 6 个氨基酸 His 标签融合,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFB protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is ≤ 1ng/ml.

Amino acid sequence

ADPLPGVGLT PSAAQTARQH PKMHLAHSTL KPAAHLIGDP SKQNSLLWRA NTDRAFLQDG FSLSNNSLLV PTSGIYFVYS QVVFSGKAYS PKATSSPLYL AHEVQLFSSQ YPFHVPLLSS QKMVYPGLQE PWLHSMYHGA AFQLTQGDQL STHTDGIPHL VLSPSTVFFG AFALHHHHHH.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • TNF b Human, His
  • TNF b Human

ProSpec-TNFA Bovine/CYT-1104,Tumor Necrosis Factor-alpha Bovine Recombinant

ProSpec-TNFA Bovine/CYT-1104,Tumor Necrosis Factor-alpha Bovine Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNFA Bovine produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 17.5kDa.

TNFA Bovine
Tumor Necrosis Factor-alpha Bovine Recombinant
CYT-1104
2µg
10µg
1mg

Catalogue number

CYT-1104

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is involved in systemic inflammationand secreted mainly by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是所有刺激急性期反应的一组细胞因子中的一员。 TNF参与全身炎症反应,主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制,TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调,特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNFA Bovine produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 17.5kDa.

TNFA is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFA 牛是一条单一的、非糖基化的多肽链,包含 158 个氨基酸,分子量为 17.5kDa。

TNFA 是通过专有的色谱技术纯化的。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The TNFA contains Phosphate buffer saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。
储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

The ED50 Is < 15 ng/ml and is measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

Amino acid sequence

MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL. 

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNF alpha human
  • TNF a Human, His
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ProSpec-TNFA Mouse, Sf9/CYT-912,Tumor Necrosis Factor-alpha Mouse Recombinant, Sf9

ProSpec-TNFA Mouse, Sf9/CYT-912,Tumor Necrosis Factor-alpha Mouse Recombinant, Sf9,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制,TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。

TNFA Mouse, Sf9
Tumor Necrosis Factor-alpha Mouse Recombinant, Sf9
CYT-912
2µg
10µg
1mg

Catalogue number

CYT-912

Synonyms

Tnfa, Tnfsf2, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子,是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制,TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调,特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNFA Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 162 amino acids and having a molecular mass of 18kDa .
TNFA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 TNFA Mouse Recombinant 是一条糖基化多肽链,包含 162 个氨基酸,分子量为 18kDa。
TNFA 在 C 末端用 6 个氨基酸的 His 标签表达,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFA protein solution contains Phosphate buffered saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALHHHH HH

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNFA Rat, His Active/CYT-1057,Tumor Necrosis Factor-alpha Rat Recombinant, His Tag Active

ProSpec-TNFA Rat, His Active/CYT-1057,Tumor Necrosis Factor-alpha Rat Recombinant, His Tag Active,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,肿瘤坏死因子是一种参与全身炎症的细胞因子,是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。

TNFA Rat, His Active

Tumor Necrosis Factor-alpha Rat Recombinant, His Tag Active
CYT-1057
ProSpec-TNFA Rat, His Active/CYT-1057,2µg
ProSpec-TNFA Rat, His Active/CYT-1057,10µg
ProSpec-TNFA Rat, His Active/CYT-1057,1mg

Catalogue number

CYT-1057

Synonyms

Tumor Necrosis Factor-alpha, TNF a His,  Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.

肿瘤坏死因子-α、TNF a His、Cachectin、TNF-α、肿瘤坏死因子配体超家族成员 2、TNF-a、N 端片段、NTF、细胞内结构域 1、细胞内结构域 2、ICD2、C 结构域 1、 C 域 2,肿瘤坏死因子,可溶性形式,Tnfa,Tnfsf2,RATTNF,Tnfa。

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子,是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制,TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调,特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNFA Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids and having a molecular mass of 19.9kDa.
TNFA Rat is expressed with an 25 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFA Rat Recombinant 是一条单一的、非糖基化的多肽链,含有 181 个氨基酸,分子量为 19.9kDa。
TNFA Rat 在 C 末端用 25 个氨基酸的 His 标签表达,并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid.

Formulation

TNFA Rat protein solution contains Phosphate Buffered Saline , 10% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is ≤ to 0.2 ng/ml.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-TNFAIP8 Human/CYT-759,Tumor Necrosis Factor, Alpha-Induced Protein 8 Human Recombinant

ProSpec-TNFAIP8 Human/CYT-759,Tumor Necrosis Factor, Alpha-Induced Protein 8 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在大肠杆菌中产生的 TNFAIP8 Human Recombinant 是一个单一的、非糖基化的多肽链,含有 221 个氨基酸,分子量为 25kDa。 TNFAIP8 在 N 末端与 23 个氨基酸的 His-tag 融合,并通过专有色谱技术进行纯化。

TNFAIP8 Human

Tumor Necrosis Factor, Alpha-Induced Protein 8 Human Recombinant
CYT-759
2µg
10µg
1mg

Catalogue number

CYT-759

Synonyms

GG2-1; MDC-3.13, SCC-S2, SCCS2, Tumor necrosis factor alpha-induced protein 8, TNF alpha-induced protein 8, Head and neck tumor and metastasis-related protein, NF-kappa-B-inducible DED-containing protein, NDED, TNF-induced protein GG2-1, TNFAIP8.

Introduction

TNFAIP8 which is a part of the TNFAIP8 family acts as a negative mediator of apoptosis and takes part in tumor progression. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, resulting in inhibition of BID cleavage and activation of caspase-3.
TNFAIP8 是 TNFAIP8 家族的一部分,作为细胞凋亡的负介质并参与肿瘤进展。 TNFAIP8 通过抑制 caspase-8 活性而不是 procaspase-8 的加工来抑制 TNF 介导的细胞凋亡,从而抑制 BID 切割和 caspase-3 的活化。

Description

TNFAIP8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 221 amino acids and having a molecular mass of 25kDa. TNFAIP8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFAIP8 Human Recombinant 是一个单一的、非糖基化的多肽链,含有 221 个氨基酸,分子量为 25kDa。 TNFAIP8 在 N 末端与 23 个氨基酸的 His-tag 融合,并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFAIP8 protein solution contains 20mM Tris-HCl buffer , 0.15M NaCl, 10% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMHSEAEE SKEVATDVFN SKNLAVQAQK KILGKMVSKS IATTLIDDTS SEVLDELYRV TREYTQNKKE AEKIIKNLIK TVIKLAILYR NNQFNQDELA LMEKFKKKVH QLAMTVVSFH QVDYTFDRNV LSRLLNECRE MLHQIIQRHL TAKSHGRVNN VFDHFSDCEF LAALYNPFGN FKPHLQKLCD GINKMLDEEN I.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TNFR (22-211) Human/CYT-851,Tumor Necrosis Factor Receptor Human Recombinant

ProSpec-TNFR (22-211) Human/CYT-851,Tumor Necrosis Factor Receptor Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

TNFR Human

Tumor Necrosis Factor Receptor Human Recombinant
CYT-851
5µg
20µg
1mg

Catalogue number

CYT-851

Synonyms

CD120a, FPF, MS5, p55, p55-R, p60, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR1-d2,TNFR55, TNFR60, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor superfamily member 1A.

Introduction

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.
TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB,介导细胞凋亡,并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应,包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

Description

TNFR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids and having a molecular mass of 23.6kDa.
TNFR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFR Human Recombinant 是一个单一的、非糖基化的多肽链,包含 213 个氨基酸,分子量为 23.6kDa。
TNFR 在 N 末端与 23 个氨基酸的 His-tag 融合,并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFR protein solution containing 20mM Tirs-HCl and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。
储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSIYPSGVI GLVPHLGDRE KRDSVCPQGK YIHPQNNSIC CTKCHKGTYL YNDCPGPGQD TDCRECESGS FTASENHLRH CLSCSKCRKE MGQVEISSCT VDRDTVCGCR KNQYRHYWSE NLFQCFNCSL CLNGTVHLSC QEKQNTVCTC HAGFFLRENE CVSCSNCKKS LECTKLCLPQ IENVKGTEDS GTT.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TNFR Human, Sf9
  • TNFR Human, His
  • TNFR Mouse
  • TNFR Human

ProSpec-TNFR Human/CYT-707,Tumor Necrosis Factor Receptor Human Recombinant

ProSpec-TNFR Human/CYT-707,Tumor Necrosis Factor Receptor Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

TNFR Human
Tumor Necrosis Factor Receptor Human Recombinant
CYT-707
5µg
20µg
1mg

Catalogue number

CYT-707

Synonyms

Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, TNF-R1, TNF-RI, TNFR-I, p60, p55, CD120a, TNFRSF1A, TNFAR, TNFR1, FPF, TBP1, TNF-R, p55-R, TNFR55, TNFR60, TNF-R-I, TNF-R55, MGC19588.

Introduction

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.
TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB,介导细胞凋亡,并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应,包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

Description

TNFR Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 162 amino acids and having a total molecular mass of 18.2 kDa. TNFR Human Recombinant is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFR Human Recombinant 是单一的、非糖基化的多肽链,包含 162 个氨基酸,总分子量为 18.2 kDa。 TNFR Human Recombinant 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The TNFR protein was lyophilized from 10mM sodium phosphate buffer pH-7.5.

Solubility

It is recommended to reconstitute the lyophilized TNFR in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFR although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNFR 虽然在室温下可稳定保存 3 周,但应在 -18C 以下干燥储存。 重组后,TNFR 应在 4C 下储存 2-7 天,以备将来在 -18C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESSGSF TASENHLRHC LSCSKCRKEM GQVEKSSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNFR Mouse
  • TNFR Human, His
  • TNFR Human, Sf9
  • TNFR Human

ProSpec-TNFR Human, His/CYT-673,Tumor Necrosis Factor Receptor Type Human Recombinant, His Tag

ProSpec-TNFR Human, His/CYT-673,Tumor Necrosis Factor Receptor Type Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在大肠杆菌中产生的 TNFR Human Recombinant 是一个单一的、非糖基化的多肽链,包含 161 个氨基酸片段,分子量为 22.68kDa,并与 4.5kDa 氨基末端的六组氨酸标签融合。 TNFR His Tag 通过专有的色谱技术进行纯化。

TNFR Human, His

Tumor Necrosis Factor Receptor Type Human Recombinant, His Tag
CYT-673
5µg
20µg
1mg

Catalogue number

CYT-673

Synonyms

Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, TNF-R1, TNF-RI, TNFR-I, p60, p55, CD120a, TNFRSF1A, TNFAR, TNFR1, FPF, TBP1, TNF-R, p55-R, TNFR55, TNFR60, TNF-R-I, TNF-R55, MGC19588.

Introduction

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.
TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB,介导细胞凋亡,并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应,包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

Description

TNFR Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 161 amino acids fragment having a molecular weight of 22.68kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The TNFR His Tag is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFR Human Recombinant 是一个单一的、非糖基化的多肽链,包含 161 个氨基酸片段,分子量为 22.68kDa,并与 4.5kDa 氨基末端的六组氨酸标签融合。 TNFR His Tag 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFR His Tag protein is supplied in 1xPBS, 50% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

DSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQ
DTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVE
ISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCL
NGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKK
SLECTKLCLPQIEN.

References

Title: β-Catenin Signaling Pathway Is Crucial for Bone Morphogenetic Protein 2 to Induce New Bone Formation*

Publication: First Published on November 3, 2006, doi:10.1074/jbc.M602700200 January 5, 2007 The Journal of Biological Chemistry,282,526-533.
Link: http://www.jbc.org/content/282/1/526.full

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-TNFR Human, Sf9/CYT-951,Tumor Necrosis Factor Receptor, sf9 Human Recombinant

ProSpec-TNFR Human, Sf9/CYT-951,Tumor Necrosis Factor Receptor, sf9 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

TNFR Human, Sf9

Tumor Necrosis Factor Receptor, sf9 Human Recombinant
CYT-951
2µg
10µg
1mg

Catalogue number

CYT-951

Synonyms

Tumor Necrosis Factor Receptor Superfamily Member 9, Tumor Necrosis Factor Receptor Superfamily, Member 9, T-Cell Antigen 4-1BB Homolog, 4-1BB Ligand Receptor, T-Cell Antigen ILA, CD137 Antigen, CDw137, CD137, ILA, Interleukin-Activated Receptor, Homolog Of Mouse Ly63,Induced By Lymphocyte Activation , Homolog Of Mouse 4-1BB, Receptor Protein 4-1BB, T Cell Antigen ILA, 4-1BB.

Introduction

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.
TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB,介导细胞凋亡,并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应,包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

Description

TNFR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 411 amino acids and having a molecular mass of 45.3kDa. .
TNFR is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 TNFR 是单条糖基化多肽链,含有 411 个氨基酸,分子量为 45.3kDa。 .
TNFR 在 C 末端用 239 个氨基酸的 hIgG-His-tag 表达,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFR protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。

对于长期储存,建议添加载体蛋白。

避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ADLFERTRSL QDPCSNCPAG TFCDNNRNQI CSPCPPNSFS SAGGQRTCDI CRQCKGVFRT RKECSSTSNA ECDCTPGFHC LGAGCSMCEQ DCKQGQELTK KGCKDCCFGT FNDQKRGICR PWTNCSLDGK SVLVNGTKER DVVCGPSPAD LSPGASSVTP PAPAREPGHS PQLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • TNFR Mouse
  • TNFR Human, His
  • TNFR Human
  • TNFR Human

ProSpec-TNFR Mouse/CYT-774,Tumor Necrosis Factor Receptor Mouse Recombinant

ProSpec-TNFR Mouse/CYT-774,Tumor Necrosis Factor Receptor Mouse Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,根据其抑制 L-929 细胞中 TNF-α 介导的细胞毒性的能力确定的 ED50 小于 1.0 µg/ml,对应于在 0.1 ng/mL rMuTNF 存在下 > 1000 IU/mg 的比活性 -一种。

TNFR Mouse

Tumor Necrosis Factor Receptor Mouse Recombinant
CYT-774
5µg
20µg
1mg

Catalogue number

CYT-774

Synonyms

Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tnfrsf1a, Tnfr-1, Tnfr1, FPF, TNF-R, TNFAR, TNFRI, p55-R, TNFR60, Tnfr-2, TNF-R-I, TNF-R55, TNFRp55, TNF-alphaR1, TNFalpha-R1.

Introduction

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.
TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB,介导细胞凋亡,并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应,包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

Description

TNFR Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.1kDa.
The TNFR is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFR Mouse Recombinant 是一条单一的、非糖基化的多肽链,含有 191 个氨基酸,分子量为 21.1kDa。
TNFR 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNFR protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized TNFR in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFR although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNFR 虽然在室温下可稳定保存 3 周,但应在 -18C 以下干燥储存。 重组后,TNFR 应在 4C 下储存 2-7 天,以备将来在 -18C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by -PAGE.

Amino acid sequence

IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A.

Biological Activity

The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1.0 µg/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 0.1 ng/mL of rMuTNF-a.

根据其抑制 L-929 细胞中 TNF-α 介导的细胞毒性的能力确定的 ED50 小于 1.0 µg/ml,对应于在 0.1 ng/mL rMuTNF 存在下 > 1000 IU/mg 的比活性 -一种。

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNFR Human
  • TNFR Human, His
  • TNFR Human, Sf9
  • TNFR Human

ProSpec-TNFR2 Human/CYT-769,Tumor Necrosis Factor Receptor Type 2 Human Recombinant

ProSpec-TNFR2 Human/CYT-769,Tumor Necrosis Factor Receptor Type 2 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNFR2.

TNFR2 Human
Tumor Necrosis Factor Receptor Type 2 Human Recombinant
CYT-769
5µg
20µg
1mg

Catalogue number

CYT-769

Synonyms

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNFR2.

Introduction

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体,对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外,在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物,介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集,它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知;然而,据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

Description

TNFR2 Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 20kDa. The TNFR2 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFR2 Human 是一条单一的、非糖基化的多肽链,含有 184 个氨基酸,分子量为 20kDa。 TNFR2 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNFR2 was Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR2,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNFR2 虽然在室温下可稳定保存 3 周,但应在 -18C 以下干燥储存。 重组后 TNFR2 应在 4C 下储存 2-7 天,以备将来在 -18C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by -PAGE.

Amino acid sequence

MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT.

Biological Activity

The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 0.2µg/ml, corresponding to a specific activity of > 5000IU/mg in the presence of 0.25ng/mL of rHuTNF-a.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNFR2 Mouse
  • TNFR2 Human Fc
  • TNFR2 Human, Sf9
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ProSpec-TNFR2 Human Fc/CYT-422,Tumor Necrosis Factor Receptor 2 Fusion Protein Human Recombinant

ProSpec-TNFR2 Human Fc/CYT-422,Tumor Necrosis Factor Receptor 2 Fusion Protein Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

TNFR2 Human Fc

Tumor Necrosis Factor Receptor 2 Fusion Protein Human Recombinant
CYT-422
10µg
50µg
1mg

Catalogue number

CYT-422

Synonyms

Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

Introduction

TNFR binds specifically to tumor necrosis factor and blocks its interaction with cell surface TNF receptors. TNF is a naturally occurring cytokine that is involved in normal inflammatory and immune responses. It plays an important role in the inflammatory processes of rheumatoid arthritis , polyarticular-course juvenile rheumatoid arthritis , and ankylosing spondylitis and the resulting joint pathology. In addition, TNF plays a role in the inflammatory process of plaque psoriasis. Elevated levels of TNF are found in involved tissues and fluids of patients with RA, psoriatic arthritis, ankylosing spondylitis , and plaque psoriasis. Two distinct receptors for TNF , a 55 kilodalton protein and a 75 kilodalton protein , exist naturally as monomeric molecules on cell surfaces and in soluble forms. Biological activity of TNF is dependent upon binding to either cell surface TNFR. Recombinant Human TNFR is a dimeric soluble form of the p75 TNF receptor that can bind to two TNF molecules.
It inhibits the activity of TNF in vitro and has been shown to affect several animal models of inflammation, including murine collagen-induced arthritis. TNFR inhibits binding of both TNF? and TNF? to cell surface TNFRs, rendering TNF biologically inactive. Cells expressing transmembrane TNF that bind to TNFR are not lysed in vitro in the presence or absence of complement.
TNFR can also modulate biological responses that are induced or regulated by TNF, including expression of adhesion molecules responsible for leukocyte migration , serum levels of cytokines , and serum levels of matrix metalloproteinase-3 .
TNFR 特异性结合肿瘤坏死因子并阻断其与细胞表面 TNF 受体的相互作用。 TNF 是一种天然存在的细胞因子,参与正常的炎症和免疫反应。它在类风湿性关节炎、多关节病程幼年类风湿性关节炎和强直性脊柱炎的炎症过程和由此产生的关节病理中起重要作用。此外,TNF 在斑块型银屑病的炎症过程中发挥作用。在 RA、银屑病关节炎、强直性脊柱炎和斑块型银屑病患者的受累组织和体液中发现 TNF 水平升高。两种不同的 TNF 受体,一种 55 千道尔顿的蛋白质和一种 75 千道尔顿的蛋白质,以单体分子的形式自然存在于细胞表面并以可溶形式存在。 TNF 的生物学活性取决于与任一细胞表面 TNFR 的结合。重组人 TNFR 是 p75 TNF 受体的二聚体可溶形式,可与两个 TNF 分子结合。
它在体外抑制 TNF 的活性,并已显示影响多种炎症动物模型,包括小鼠胶原蛋白诱导的关节炎。 TNFR 抑制两种 TNF 的结合?和肿瘤坏死因子?到细胞表面的 TNFR,使 TNF 失去生物活性。在存在或不存在补体的情况下,表达与 TNFR 结合的跨膜 TNF 的细胞不会在体外裂解。
TNFR 还可以调节由 TNF 诱导或调节的生物反应,包括负责白细胞迁移的粘附分子的表达、细胞因子的血清水平和基质金属蛋白酶-3 的血清水平。

Description

Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilo Dalton tumor necrosis factor receptor 2 linked to the Fc portion of human IgG1. The Fc component of TNFR2 contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilo Daltons.
The TNFR2 is purified by standard chromatographic techniques.
在 CHO 中产生的重组人肿瘤坏死因子受体 2 融合蛋白是一种二聚体、糖基化的多肽链,由与人 IgG1 的 Fc 部分连接的人 75 千道尔顿肿瘤坏死因子受体 2 的细胞外配体结合部分组成。 TNFR2 的 Fc 成分包含 CH2 结构域、CH3 结构域和铰链区,但不包含 IgG1 的 CH1 结构域。 它由 934 个氨基酸组成,表观分子量约为 150 千道尔顿。
TNFR2通过标准色谱技术纯化。

Source

Chinese Hamster Ovarian Cells .

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Each mg contains 1.6mg mannitol, 0.4 mg sucrose and 48 µg tromethamine.

Solubility

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Tumor Necrosis Factor Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFR2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子受体虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,TNFR2 应在 4°C 下储存 2-7 天,以备将来在低于 -18°C 的温度下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Biological Activity

Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a Specific Activity of 17,000,000 IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNFR2 Mouse
  • TNFR2 Human
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ProSpec-TNFR2 Human, His/CYT-674,Tumor Necrosis Factor Receptor Type 2 Human Recombinant, His Tag

ProSpec-TNFR2 Human, His/CYT-674,Tumor Necrosis Factor Receptor Type 2 Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

TNFR2 Human, His
Tumor Necrosis Factor Receptor Type 2 Human Recombinant, His Tag
CYT-674
5µg
20µg
1mg

Catalogue number

CYT-674

Synonyms

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b, Etanercept, TNF-R2, TNF-RII, TNFR-II, TNFRSF1B, TNFBR, TNFR2, TBPII, TNFR2, TNFR1B, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

Introduction

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体,对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外,在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物,介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集,它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知;然而,据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

Description

TNFR2 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 184 amino acids fragment having a molecular weight of 24.45kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The TNFR2 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFR2 Human Recombinant 是一个单一的、非糖基化的多肽链,包含 184 个氨基酸片段,分子量为 24.45kDa,并与 4.5kDa 氨基末端的六组氨酸标签融合。
TNFR2 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFR2 protein is supplied in 20mM Tris HCl pH-8, 5mM EDTA and 50% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。
储存,在 -20°C 下冷冻更长时间。
请避免冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWV
PECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSD
VVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPT.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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  • TNFR2 Mouse

ProSpec-TNFR2 Human, Sf9/CYT-908,Tumor Necrosis Factor Receptor Type 2 Human Recombinant, Sf9

ProSpec-TNFR2 Human, Sf9/CYT-908,Tumor Necrosis Factor Receptor Type 2 Human Recombinant, Sf9,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在 Sf9 杆状病毒细胞中产生的 TNFR2 是一条糖基化多肽链,在 C 端与一个 6 个氨基酸的 His 标签融合,共包含 241 个氨基酸,分子量为 25.9kDa。

TNFR2 Human, Sf9

Tumor Necrosis Factor Receptor Type 2 Human Recombinant, Sf9
CYT-908
2µg
10µg
1mg

Catalogue number

CYT-908

Synonyms

Tumor Necrosis Factor Receptor Superfamily, Member 1B, TNFR2, TNFBR, Tumor Necrosis Factor Receptor Type II, Tumor Necrosis Factor Receptor 2, P80 TNF-Alpha Receptor, TNF-RII, TNF-R2, P75, Tumor Necrosis Factor Receptor Superfamily Member 1B, Tumor Necrosis Factor Binding Protein 2, Tumor Necrosis Factor Beta Receptor, Soluble TNFR1B Variant 1, P75 TNF Receptor, CD120b Antigen, Etanercept, TNF-R-II, TNF-R75, P75TNFR, TNFR-II, CD120b, TNFR1B, TNFR80, TBPII.

Introduction

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体,对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外,在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物,介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集,它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知;然而,据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

Description

TNFR2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain and fused to a 6 aa His Tag at C-terminus containing a total of 241 amino acids and having a molecular mass of 25.9kDa.
TNFR2 shows multiple bands between 28-40kDa on -PAGE, reducing conditions and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 TNFR2 是一条糖基化多肽链,在 C 端与一个 6 个氨基酸的 His 标签融合,共包含 241 个氨基酸,分子量为 25.9kDa。
TNFR2 在 -PAGE 上显示 28-40kDa 之间的多个条带,还原条件并通过专有色谱技术纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TNFR2 protein solution contains Phosphate buffered saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

LPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPTRSMAPG AVHLPQPVST RSQHTQPTPE PSTAPSTSFL LPMGPSPPAE GSTGDHHHHH H

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TNFR2 Human, His
  • TNFR2 Human
  • TNFR2 Mouse
  • TNFR2 Human Fc

ProSpec-TNFR2 Mouse/CYT-770,Tumor Necrosis Factor Receptor Type 2 Mouse Recombinant

ProSpec-TNFR2 Mouse/CYT-770,Tumor Necrosis Factor Receptor Type 2 Mouse Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b, Tnfrsf1b, Tnfr-2, Tnfr2, TNFBR, TNFR80, TNFRII, TNF-R75, TNF-R-II, TNF-alphaR2, TNFalpha-R2.

TNFR2 Mouse

Tumor Necrosis Factor Receptor Type 2 Mouse Recombinant
CYT-770
5µg
20µg
1mg

Catalogue number

CYT-770

Synonyms

Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b, Tnfrsf1b, Tnfr-2, Tnfr2, TNFBR, TNFR80, TNFRII, TNF-R75, TNF-R-II, TNF-alphaR2, TNFalpha-R2.

Introduction

TNFR2 belongs to the TNF-receptor superfamily. TNFR2 is receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. TNFR2 mediates the majority of the metabolic effects of TNF-alpha. In addition, knockout studies in mice propose a role for TNFR2 in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2 expression might have a significant role in the angiogenesis, tumor cell proliferation and metastasis of Invasive micropapillary carcinoma of the breast.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR2 属于 TNF 受体超家族。 TNFR2 是对 TNFSF2/TNF-α 具有高亲和力的受体,对同源三聚体 TNFSF1/淋巴毒素-α 的亲和力低约 5 倍。 TNFR2 介导 TNF-α 的大部分代谢作用。此外,在小鼠中进行的敲除研究提出了 TNFR2 通过刺激抗氧化途径保护神经元免于凋亡的作用。 TNFR2的表达可能在乳腺浸润性微乳头状癌的血管生成、肿瘤细胞增殖和转移中发挥重要作用。
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物,介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集,它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知;然而,据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。

Description

TNFR2 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 236 amino acids and having a molecular mass of 25.3kDa.
The TNFR2 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR2 Mouse Recombinant 是单条非糖基化多肽链,含有 236 个氨基酸,分子量为 25.3kDa。
TNFR2 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNFR2 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR2,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNFR2 虽然在室温下可稳定保存 3 周,但应在 -18C 以下干燥储存。 重组后 TNFR2 应在 4C 下储存 2-7 天,以备将来在 -18C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by -PAGE.

Amino acid sequence

VPAQVVLTPY KPEPGYECQI SQEYYDRKAQ MCCAKCPPGQ YVKHFCNKTS DTVCADCEAS MYTQVWNQFR TCLSCSSSCT TDQVEIRACT KQQNRVCACE AGRYCALKTH SGSCRQCMRL SKCGPGFGVA SSRAPNGNVL CKACAPGTFS DTTSSTDVCR PHRICSILAI PGNASTDAVC APESPTLSAI PRTLYVSQPE PTRSQPLDQE PGPSQTPSIL TSLGSTPIIE QSTKGG.

Biological Activity

The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 2µg/ml, corresponding to a specific activity of > 500IU/mg in the presence of 0.1ng/mL of rHuTNF-a.

根据其抑制 L-929 细胞中 TNF-a 介导的细胞毒性的能力所确定的 ED50 小于 2μg/ml,对应于在 0.1ng/mL rHuTNF-a 存在下 > 500IU/mg 的比活性 .

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNFR2 Human, His
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  • TNFR2 Human Fc

ProSpec-TNFRSF10A Human Fc/CYT-877,TRAIL Receptor-1 Human Recombinant Fc

ProSpec-TNFRSF10A Human Fc/CYT-877,TRAIL Receptor-1 Human Recombinant Fc,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在大肠杆菌中产生的 TRAIL R1 Fc 人重组体是一种非糖基化、二硫键连接的同源二聚体,含有两条多肽链,每条 217 个氨基酸,每条链的分子量为 41kDa

TNFRSF10A Human Fc

TRAIL Receptor-1 Human Recombinant Fc
CYT-877
10µg
50µg
1mg

Catalogue number

CYT-877

Synonyms

Tumor Necrosis Factor Receptor Superfamily Member 10a, TRAILR1, APO2, DR4, TNF-Related Apoptosis-Inducing Ligand Receptor 1, Death Receptor 4, TRAIL Receptor 1, TRAIL-R1, Tumor Necrosis Factor Receptor Superfamily Member 10a Variant 2, Tumor Necrosis Factor Receptor Superfamily Member 10A, Cytotoxic TRAIL Receptor, CD261 Antigen, TRAILR-1, CD261, TNFRSF10A.

Introduction

Human TNFRSF10A is a type 1 transmembrane protein in the TNF R superfamily. TNFRSF10A is not expressed in rodents. The trimeric ligand Trail binds TNFRSF10A and induces apoptosis, and recombinant, soluble forms of the receptor inhibit Trail-induced apoptosis. TNFRSF10A is expressed generally in damaged, infected, and malignant cells. TNFRSF10A functions in immune surveillance, inducing apoptosis in cancer cells but not normal cells.
人 TNFRSF10A 是 TNF R 超家族中的 1 型跨膜蛋白。 TNFRSF10A 在啮齿动物中不表达。 三聚体配体 Trail 结合 TNFRSF10A 并诱导细胞凋亡,而重组的可溶性受体形式可抑制 Trail 诱导的细胞凋亡。 TNFRSF10A 通常在受损、感染和恶性细胞中表达。 TNFRSF10A 在免疫监视中起作用,诱导癌细胞而非正常细胞的凋亡。

Description

TRAIL R1 Fc Human Recombinant produced in E.Coli is a non-glycosylated, disulfide-linked homodimer containing two polypeptide chains of 217 amino acids each and having a molecular mass of 41kDa [including tag] each chain.
TRAILR1 is fused to a 236 amino acid Fc tag at C-terminal and purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TRAIL R1 Fc 人重组体是一种非糖基化、二硫键连接的同源二聚体,含有两条多肽链,每条 217 个氨基酸,每条链的分子量为 41kDa [包括标签]。
TRAILR1 在 C 末端与 236 个氨基酸的 Fc 标签融合,并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered solution in PBS.

Solubility

It is recommended to reconstitute the lyophilized TNFRSF10A in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TNFRSF10A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TRAIL-R1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNFRSF10A 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,TRAIL-R1 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ASGTEAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASNNLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHNIEGR MDPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK.

Biological Activity

The ED50, as measured by its ability to inhibit Trail-mediated cytotoxicity in mouse L-929 cells, is less than 1.25ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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  • TNFRSF10B Human
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ProSpec-TNFRSF10B Human/CYT-069,TNF Ligand Receptor Superfamily Member 10B Human Recombinant

ProSpec-TNFRSF10B Human/CYT-069,TNF Ligand Receptor Superfamily Member 10B Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNFRSF10B 通过中和新鲜人 PBMC 中内源性 TRAIL 的能力来减少 LPS 诱导的 TNF 的产生。 在该试验中,内源性 TRAIL 在 24 小时暴露于 LPS 期间被诱导,但在 TNFRSF10B 存在下,TRAIL 诱导的 TNF 被抑制。

TNFRSF10B Human

TNF Ligand Receptor Superfamily Member 10B Human Recombinant
CYT-069
10µg
50µg
1mg

Catalogue number

CYT-069

Synonyms

Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, CD262, TNFRSF10B, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, TRICKB, TRICK2A, TRICK2B, KILLER/DR5.

Introduction

TRAIL Receptor-1 and TRAIL Receptor-2 are members of the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which is capable of activating the cell’s apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L.
TRAIL Receptor-1 和 TRAIL Receptor-2 是跨膜蛋白 TNFR 超家族的成员,含有细胞质“死亡结构域”,能够激活细胞的凋亡机制。 这些受体通过与膜锚定或可溶性 TRAIL/Apo2L 结合而被激活。

Description

TNFRSF10B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 132 amino acids and having a molecular mass of 14.8kDa.
The TNFRSF10B is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFRSF10B 人重组体是一种单一的、非糖基化的多肽链,含有 132 个氨基酸,分子量为 14.8kDa。
TNFRSF10B 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TNFRSF10B in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 TNFRSF10B 溶解在不低于 100µg/ml 的无菌 18M-cm H2O 中,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFRSF10B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF10B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNFRSF10B 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,TNFRSF10B 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ESALITQQD LAPQQRVAPQ QKRSSPSEGL CPPGHHISED GRDCISCKYG QDYSTHWNDL LFCLRCTRCD SGEVELSPCT TTRNTVCQCE EGTFREEDSP EMCRKCRTGC PRGMVKVGDC TPWSDIECVH KES.

Biological Activity

The TNFRSF10B reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS but in the presence of TNFRSF10B, TRAIL-induced TNF is suppressed.
TNFRSF10B 通过中和新鲜人 PBMC 中内源性 TRAIL 的能力来减少 LPS 诱导的 TNF 的产生。 在该试验中,内源性 TRAIL 在 24 小时暴露于 LPS 期间被诱导,但在 TNFRSF10B 存在下,TRAIL 诱导的 TNF 被抑制。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TNFRSF10A Human Fc
  • TNFRSF10B Human, Sf9

ProSpec-TNFRSF10B Human, Sf9/CYT-1055,TNF Ligand Receptor Superfamily Member 10B Human Recombinant, Sf9

ProSpec-TNFRSF10B Human, Sf9/CYT-1055,TNF Ligand Receptor Superfamily Member 10B Human Recombinant, Sf9,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,使用 Jurkat 人 T 淋巴细胞通过其抑制细胞毒性测定的能力来测量。 此效果的 ED50 ≤ 至 5ng/ml 与 TRAIL

TNFRSF10B Human, Sf9
TNF Ligand Receptor Superfamily Member 10B Human Recombinant, Sf9
CYT-1055
2µg
10µg
100µg

Catalogue number

CYT-1055

Synonyms

TNFRSF10B, CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2.

Introduction

TRAIL Receptor-1 and TRAIL Receptor-2 are members of the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which is capable of activating the cell’s apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L.

TRAIL Receptor-1 和 TRAIL Receptor-2 是跨膜蛋白 TNFR 超家族的成员,含有细胞质“死亡结构域”,能够激活细胞的凋亡机制。 这些受体通过与膜锚定或可溶性 TRAIL/Apo2L 结合而被激活。

Description

TNFRSF10B produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 394 amino acids and having a molecular mass of 43.9kDa. .
TNFRSF10B is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF10B 是单条糖基化多肽链,包含 394 个氨基酸,分子量为 43.9kDa。 .
TNFRSF10B 在 C 末端带有 239 个氨基酸的 hIgG-His 标签,并通过专有色谱技术进行纯化。

Source

Sf9, Insect cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF10B protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。 对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Biological Activity

Measured by its ability to inhibit cytotoxicity assay using Jurkat human T lymphocyte. The ED50 for this effect ≤ to 5ng/ml with TRAIL

使用 Jurkat 人 T 淋巴细胞通过其抑制细胞毒性测定的能力来测量。 此效果的 ED50 ≤ 至 5ng/ml 与 TRAIL

.

Amino acid sequence

ITQQDLAPQQ RAAPQQKRSS PSEGLCPPGH HISEDGRDCI SCKYGQDYST HWNDLLFCLR CTRCDSGEVE LSPCTTTRNT VCQCEEGTFR EEDSPEMCRK CRTGCPRGMV KVGDCTPWSD IECVHKESGT KHSGEVPAVE ETVTSSPGTP ASPCSLEPKS CDKTHTCPPC PAPELLGGPS
VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ
GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • TNFRSF10B Human

ProSpec-TNFRSF17 Human, Sf9/CYT-1148

ProSpec-TNFRSF17 Human Sf9,ProSpec CYT-1148,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,B 细胞成熟抗原或 TNFRSF17 或肿瘤坏死因子受体超家族成员 17,是 TNF 受体蛋白家族的一部分。 这种蛋白质是 TNFSF13B/BLyS/BAFF、TNFSF13/APRIL 和促进 B 细胞存活受体。

TNFRSF17 Human, Sf9

B-Cell Maturation Antigen, Sf9 Human Recombinant
CYT-1148
ProSpec-TNFRSF17 Human, Sf9/CYT-1148 2µg
ProSpec-TNFRSF17 Human, Sf9/CYT-1148 10µg
ProSpec-TNFRSF17 Human, Sf9/CYT-1148 1mg

Catalogue number

CYT-1148

Synonyms

BCMA, CD269, Tumor Necrosis Factor Receptor Superfamily Member 17, BCM, TNFRSF17, B-cell maturation protein, CD269 antigen

Introduction

B-Cell Maturation Antigen or TNFRSF17 or tumor necrosis factor receptor superfamily member 17, is part of the TNF receptor protein family. This protein is a TNFSF13B/BLyS/BAFF, TNFSF13/APRIL & promotes B-cell survival receptor. TNFRSF17 has a crucial part in the humoral immunity regulation. This protein is responsible for the activation of NF-kappa-B & JNK.

B 细胞成熟抗原或 TNFRSF17 或肿瘤坏死因子受体超家族成员 17,是 TNF 受体蛋白家族的一部分。 这种蛋白质是 TNFSF13B/BLyS/BAFF、TNFSF13/APRIL 和促进 B 细胞存活受体。 TNFRSF17 在体液免疫调节中起着至关重要的作用。 这种蛋白质负责激活 NF-kappa-B 和 JNK。

Description

TNFRSF17 Human Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 296 amino acids and having a molecular mass of  33.1Da.
TNFRSF17 is fused to a 242 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

杆状病毒产生的 TNFRSF17 Human Recombinant 是单条糖基化多肽链,包含 296 个氨基酸,分子量为 33.1Da。
TNFRSF17 在 C 端与 242 个氨基酸的 hIgG-His-Tag 融合,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF17 protein contains 10% glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ADPMLQMAGQ CSQNEYFDSL LHACIPCQLR CSSNTPPLTC QRYCNASVTN SVKGTNALEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • TNFRSF17 Human
  • TNFRSF17 Human, His

ProSpec-TNFSF14 Human/CYT-1202

ProSpec-TNFSF14 Human,ProSpec CYT-1202,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,TNFRSF14 是 TNF 受体超家族的成员,是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。

ProSpec-TNFSF14 Human/CYT-1202

Catalogue number

CYT-1202

Synonyms

Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light

Introduction

TNFRSF14, a member of the TNF receptor superfamily, is a type I transmembrane protein. TNFRSF14 is expressed in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. TNFRSF14 operates as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. Additionally, TNFRSF14 encourages the proliferation of T cells, and triggers apoptosis of various tumor cells.

TNFRSF14 是 TNF 受体超家族的成员,是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。 TNFRSF14 作为激活淋巴样细胞的共刺激因子和对疱疹病毒感染的威慑物起作用。 此外,TNFRSF14 促进 T 细胞的增殖,并引发各种肿瘤细胞的凋亡。

Description

Recombinant Human LIGHT having a Mw of 43kDa was purified from E. coli.
The Recombinant Human LIGHT is fused to GST tag at its N terminal and purified by proprietary chromatographic technique.

从大肠杆菌中纯化具有 43kDa 分子量的重组人 LIGHT。
Recombinant Human LIGHT 在其 N 末端与 GST 标签融合,并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNFSF14 solution contains 25mM Tris-Base/ 25mM K2CO3.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Amino acid sequence

DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV

Purity

Protein is >95% pure as determined by 10% PAGE .

Applications

Immunoassay.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TNFSF14 Mouse

ProSpec-TNFSF14 Mouse/CYT-826

ProSpec-TNFSF14 Mouse,ProSpec CYT-826,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,TNFRSF14 是 TNF 受体超家族的成员,是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。

TNFSF14 Mouse

LIGHT Mouse Recombinant
CYT-826
ProSpec-TNFSF14 Mouse/CYT-826 5µg
ProSpec-TNFSF14 Mouse/CYT-826 20µg
ProSpec-TNFSF14 Mouse/CYT-826 1mg

Catalogue number

CYT-826

Synonyms

Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light.

Introduction

TNFRSF14, a member of the TNF receptor superfamily, is a type I transmembrane protein. TNFRSF14 is expressed in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. TNFRSF14 operates as a co-stimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. Additionally, TNFRSF14 encourages the proliferation of T cells, and triggers apoptosis of various tumor cells.
TNFRSF14 是 TNF 受体超家族的成员,是一种 I 型跨膜蛋白。 TNFRSF14 在外周血 T 细胞、B 细胞、单核细胞和富含淋巴细胞的各种组织中表达。 TNFRSF14 作为激活淋巴样细胞的共刺激因子和对疱疹病毒感染的威慑物起作用。 此外,TNFRSF14 促进 T 细胞的增殖,并引发各种肿瘤细胞的凋亡。

Description

TNFSF14 Mouse Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 168 amino acids and having a molecular mass of 18.4kDa.
在大肠杆菌中产生的 TNFSF14 Mouse Recombinant 是一种单一的、非糖基化的多肽链,含有 168 个氨基酸,分子量为 18.4kDa。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNFSF14 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TNFSF14 in sterile 100mM HAc not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 TNFSF14 溶解在不低于 100µg/ml 的无菌 100mM HAc 中,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNFSF14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFSF14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNFSF14 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后 TNFSF14 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 96.0% as determined by:
Analysis by RP-HPLC.

Analysis by -PAGE.

Amino acid sequence

DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV.

Biological Activity

The ED50 as determined by a cytotoxicity assay using human HT-29 cells is less than 2µg/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-g.
通过使用人 HT-29 细胞的细胞毒性测定法确定的 ED50 小于 2μg/ml,对应于在鼠抗多组氨酸单克隆抗体和 rHuIFN-g 存在下 > 500 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNFRSF14 Human, Sf9
  • TNFRSF14 Human, His
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ProSpec-SCGB1A1 Human/CYT-743

ProSpec-SCGB1A1 Human,ProSpec CYT-743,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,子宫珠蛋白属于分泌珠蛋白超家族,是一种多功能蛋白,通过结合磷脂和前列腺素等疏水小分子发挥抗炎和抗肿瘤作用。 子宫珠蛋白参与多种功能,包括抗炎、抑制磷脂酶 A2 和螯合疏水配体。

SCGB1A1 Human

Uteroglobin Human Recombinant
CYT-743
ProSpec-SCGB1A1 Human/CYT-743 10µg
ProSpec-SCGB1A1 Human/CYT-743 50µg
ProSpec-SCGB1A1 Human/CYT-743 1mg

Catalogue number

CYT-743

Synonyms

Uteroglobin, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, Secretoglobin family 1A member 1, Urinary protein 1, UP-1, UP1, Urine protein 1, SCGB1A1, CCSP, UGB, CC16.

Introduction

Uteroglobin which belongs to the Secretoglobin superfamily, is a multifunctional protein that exerts anti-inflammatory and anti-tumorigenic effects by binding small hydrophobic molecules such as phospholipids and prostaglandins. Uteroglobin is involved in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. SCGB1A1 is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia which communicate with the external environment. On top of sequestering pro-inflammatory mediators and carcinogens, Uteroglobin is implicated in the inhibition of cell migration and invasion, platelet aggregation, and T cell differentiation. SCGB1A1 gene defects are associated with a susceptibility to asthma.
子宫珠蛋白属于分泌珠蛋白超家族,是一种多功能蛋白,通过结合磷脂和前列腺素等疏水小分子发挥抗炎和抗肿瘤作用。 子宫珠蛋白参与多种功能,包括抗炎、抑制磷脂酶 A2 和螯合疏水配体。 SCGB1A1 由 Clara 细胞(主要存在于肺细支气管中的非纤毛、非粘液分泌细胞)和与外部环境通信的其他上皮细胞表达。 除了隔离促炎介质和致癌物外,子宫珠蛋白还参与抑制细胞迁移和侵袭、血小板聚集和 T 细胞分化。 SCGB1A1 基因缺陷与哮喘易感性有关。

Description

Uteroglobin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 15.8kDa.
The SCGB1A1 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The Uteroglobin protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized SCGB1A1 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Uteroglobin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCGB1A1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干子宫珠蛋白虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,SCGB1A1 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

EICPSFQRVI ETLLMDTPSS YEAAMELFSP DQDMREAGAQ LKKLVDTLPQ KPRESIIKLM EKIAQSSLCN.

Biological Activity

The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0µg/ml, corresponding to a specific activity of > 200 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SCGB1A1 Mouse
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  • SCGB1A1 Human, His

ProSpec-SCGB1A1 Mouse/CYT-746

ProSpec-SCGB1A1 Mouse,ProSpec CYT-746,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.

SCGB1A1 Mouse

Uteroglobin Mouse Recombinant
CYT-746
ProSpec-SCGB1A1 Mouse/CYT-746 10µg
ProSpec-SCGB1A1 Mouse/CYT-746 50µg
ProSpec-SCGB1A1 Mouse/CYT-746 1mg

Catalogue number

CYT-746

Synonyms

Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.

Introduction

Uteroglobin which belongs to the Secretoglobin superfamily, is a multifunctional protein that exerts anti-inflammatory and anti-tumorigenic effects by binding small hydrophobic molecules such as phospholipids and prostaglandins. Uteroglobin is involved in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. SCGB1A1 is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia which communicate with the external environment. On top of sequestering pro-inflammatory mediators and carcinogens, Uteroglobin is implicated in the inhibition of cell migration and invasion, platelet aggregation, and T cell differentiation. SCGB1A1 gene defects are associated with a susceptibility to asthma.
子宫珠蛋白属于分泌珠蛋白超家族,是一种多功能蛋白,通过结合磷脂和前列腺素等疏水小分子发挥抗炎和抗肿瘤作用。 子宫珠蛋白参与多种功能,包括抗炎、抑制磷脂酶 A2 和螯合疏水配体。 SCGB1A1 由 Clara 细胞(主要存在于肺细支气管中的非纤毛、非粘液分泌细胞)和与外部环境通信的其他上皮细胞表达。 除了隔离促炎介质和致癌物外,子宫珠蛋白还参与抑制细胞迁移和侵袭、血小板聚集和 T 细胞分化。 SCGB1A1 基因缺陷与哮喘易感性有关。

Description

Uteroglobin Mouse Recombinant produced in E.coli is a non-glycosylated disulfide-linked homodimeric protein containing 2×75 amino acids chains and having a molecular mass of 16.7kDa.
The SCGB1A1 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Uteroglobin Mouse Recombinant 是一种非糖基化的二硫键连接的同型二聚体蛋白,含有 2×75 个氨基酸链,分子量为 16.7kDa。
SCGB1A1 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The Uteroglobin protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized SCGB1A1 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Uteroglobin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCGB1A1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干子宫珠蛋白虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,SCGB1A1 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

DICPGFLQVL EALLMESESG YVASLKPFNP GSDLQNAGTQ LKRLVDTLPQ ETRINIMKLT EKILTSPLCK QDLRF.

Biological Activity

The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0µg/ml, corresponding to a specific activity of > 200 IU/mg.

由固定化蛋白质支持 A549 人肺癌细胞粘附的能力确定的 ED50 小于 5.0μg/ml,对应于 > 200 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SCGB1A1 Human, His
  • SCGB1A1 Human
  • SCGB1A1 Rat

ProSpec-SCGB1A1 Rat/CYT-820

ProSpec-SCGB1A1 Rat,ProSpec CYT-820,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.

SCGB1A1 Rat

Uteroglobin Rat Recombinant
CYT-820
ProSpec-SCGB1A1 Rat/CYT-820 10µg
ProSpec-SCGB1A1 Rat/CYT-820 50µg
ProSpec-SCGB1A1 Rat/CYT-820 1mg

Catalogue number

CYT-820

Synonyms

Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP.

Introduction

Uteroglobin which belongs to the Secretoglobin superfamily, is a multifunctional protein that exerts anti-inflammatory and anti-tumorigenic effects by binding small hydrophobic molecules such as phospholipids and prostaglandins. Uteroglobin is involved in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. SCGB1A1 is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia which communicate with the external environment. On top of sequestering pro-inflammatory mediators and carcinogens, Uteroglobin is implicated in the inhibition of cell migration and invasion, platelet aggregation, and T cell differentiation. SCGB1A1 gene defects are associated with a susceptibility to asthma.
子宫珠蛋白属于分泌珠蛋白超家族,是一种多功能蛋白,通过结合磷脂和前列腺素等疏水小分子发挥抗炎和抗肿瘤作用。 子宫珠蛋白参与多种功能,包括抗炎、抑制磷脂酶 A2 和螯合疏水配体。 SCGB1A1 由 Clara 细胞(主要存在于肺细支气管中的非纤毛、非粘液分泌细胞)和与外部环境通信的其他上皮细胞表达。 除了隔离促炎介质和致癌物外,子宫珠蛋白还参与抑制细胞迁移和侵袭、血小板聚集和 T 细胞分化。 SCGB1A1 基因缺陷与哮喘易感性有关。

Description

SCGB1A1 Rat Recombinant produced in E.Coli is a homodimeric non-glycosylated polypeptide chains consisting of two 77 amino acids and having a molecular mass of 17.0kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

SCGB1A1 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized SCGB1A1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 SCGB1A1,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized SCGB1A1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCGB1A1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SCGB1A1 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,SCGB1A1 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。 对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

SSDICPGFLQ VLEALLLGSE SNYEAALKPF NPASDLQNAG TQLKRLVDTL PQETRINIVK LTEKILTSPL CEQDLRV

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 ?g/ml, corresponding to a specific activity of >200IU/mg.

与标准相比具有完全的生物活性。 由固定化蛋白质支持 A549 人肺癌细胞粘附的能力所确定的 ED50 小于 5.0 μg/ml,对应于 >200IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • SCGB1A1 Mouse
  • SCGB1A1 Human
  • SCGB1A1 Human, His

ProSpec-Visfatin Human, His/CYT-563

ProSpec-Visfatin Human His,ProSpec CYT-563,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,过度肥胖是发生胰岛素抵抗和 2 型糖尿病的最重要风险。脂肪组织产生几种蛋白质,如瘦素、脂联素、抵抗素、肿瘤坏死因子-α和 IL-6,它们调节胰岛素敏感性,似乎在胰岛素抵抗、糖尿病、血脂异常、炎症和动脉粥样硬化的发病机制中起重要作用.然而,脂肪组织诱导胰岛素抵抗的机制以及脂肪细胞因子在 T2DM 发病机制中的作用尚未完全确定。

Visfatin Human, His

Visfatin Recombinant Human, His Tag
CYT-563
ProSpec-Visfatin Human, His/CYT-563 5µg
ProSpec-Visfatin Human, His/CYT-563 25µg
ProSpec-Visfatin Human, His/CYT-563 1mg

Catalogue number

CYT-563

Synonyms

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Introduction

Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus . Adipose tissue produces several proteins such as leptin, adiponectin, resistin, tumor necrosis factor-?, and IL-6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established.
Visfatin, also known as pre-B cell colony-enhancing factor , is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulin-deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.
过度肥胖是发生胰岛素抵抗和 2 型糖尿病的最重要风险。脂肪组织产生几种蛋白质,如瘦素、脂联素、抵抗素、肿瘤坏死因子-α和 IL-6,它们调节胰岛素敏感性,似乎在胰岛素抵抗、糖尿病、血脂异常、炎症和动脉粥样硬化的发病机制中起重要作用.然而,脂肪组织诱导胰岛素抵抗的机制以及脂肪细胞因子在 T2DM 发病机制中的作用尚未完全确定。
Visfatin,又称pre-B细胞集落增强因子,是一种在内脏脂肪中高度表达的细胞因子,最初作为一种分泌因子被分离出来,与IL-7和干细胞因子协同促进B细胞前体的生长.已在鲤鱼、无脊椎动物软体动物和细菌以及脊椎动物(包括人类和小鼠)中鉴定出 Visfatin 同源物。它被假定在先天免疫中发挥作用。
Visfatin 在刺激肌肉和脂肪细胞葡萄糖转运以及抑制肝细胞葡萄糖产生方面发挥剂量依赖性和数量上与胰岛素相似的胰岛素模拟作用。在小鼠中静脉注射重组内脂素以剂量依赖性方式降低血浆葡萄糖。与其类似胰岛素的作用相一致,visfatin 在降低胰岛素缺陷型糖尿病小鼠的高血糖方面与胰岛素一样有效。还发现 Visfatin 与胰岛素受体结合并激活胰岛素受体,导致受体磷酸化和下游信号分子的激活。然而,内脂素和胰岛素没有竞争与胰岛素受体的结合,表明这两种蛋白质被受体的不同区域识别。因此,内脂素可能在葡萄糖稳态和生物合成或信号转导中的失调中发挥作用,并可能有助于糖尿病的发病机制。

Description

Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 511 amino acids and having a molecular mass of 57 kDa.
The recombinant human Visfatin is fused to His tag at N-Terminus.
在大肠杆菌中产生的 Visfatin Human Recombinant 是一种单一的、非糖基化的多肽链,含有 511 个氨基酸,分子量为 57 kDa。
重组人 Visfatin 在 N 末端与 His 标签融合。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered solution at a concentration of 1mg/ml.

Formulation

Visfatin His tag protein contains 20mM Tris pH-8, 0.1mM DTT & 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。
储存,在 -20°C 下冷冻更长时间。
请避免冻融循环。

Purity

Greater than 95.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG WNYILEKYDG HLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVV YDIYNACEKI WGEDLRHLIV SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-WISP2 Human/CYT-970

ProSpec-WISP2 Human,ProSpec CYT-970,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,WNT1 Inducible Signaling Pathway Protein 2, Connective Tissue Growth Factor-Related Protein 58, Connective Tissue Growth Factor-Like Protein, CCN Family Member 5, CTGF-L, CT58, CCN5, WISP-2, CTGFL, WISP2.

WISP2 Human

WNT1 Inducible Signaling Pathway Protein 2 Human Recombinant
CYT-970
ProSpec-WISP2 Human/CYT-970 5µg
ProSpec-WISP2 Human/CYT-970 20µg
ProSpec-WISP2 Human/CYT-970 1mg

Catalogue number

CYT-970

Synonyms

WNT1 Inducible Signaling Pathway Protein 2, Connective Tissue Growth Factor-Related Protein 58, Connective Tissue Growth Factor-Like Protein, CCN Family Member 5, CTGF-L, CT58, CCN5, WISP-2, CTGFL, WISP2.

Introduction

WNT1-inducible-signaling pathway protein 2 belongs to the WNT1 inducible signaling pathway protein subfamily, which belongs to the connective tissue growth factor family. The CTGF family members are characterized by 4 conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. WISP2 protein lacks the CT domain which is implicated in dimerization binding. WISP2 is possibly involved in bone remodeling. WISP2 is expressed in primary osteoblasts and fibroblasts. WISP2 stimulates osteoblast adhesion and inhibits osteocalcin production. WISP2 expression in colon tumors is reduced while the other 2 WISP members are overexpressed in colon tumors. WISP2 may play an imperative role in modulating bone turnover.

WNT1诱导信号通路蛋白2属于WNT1诱导信号通路蛋白亚家族,属于结缔组织生长因子家族。 CTGF 家族成员的特征在于 4 个保守的富含半胱氨酸结构域:胰岛素样生长因子结合结构域、von Willebrand 因子 C 型模块、血小板反应蛋白结构域和 C 末端胱氨酸结样结构域。 WISP2 蛋白缺乏与二聚化结合有关的 CT 结构域。 WISP2 可能参与骨重塑。 WISP2 在原代成骨细胞和成纤维细胞中表达。 WISP2 刺激成骨细胞粘附并抑制骨钙素的产生。 WISP2 在结肠肿瘤中的表达减少,而其他 2 个 WISP 成员在结肠肿瘤中过度表达。 WISP2 可能在调节骨转换中发挥重要作用。

Description

WISP2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing a total of 228 amino acids and having a molecular mass of 24.4kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid .

Solubility

It is recommended to reconstitute the lyophilized WISP-2 in sterile 10mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized WISP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution WISP-2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 WISP2 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重构后,WISP-2 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-IL6ST Human/CYT-1156

ProSpec-IL6ST Human,ProSpec CYT-1156,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在 Sf9 杆状病毒细胞中产生的 IL6ST Human 是单条糖基化多肽链,含有 605 个氨基酸,分子量为 68.9kDa。IL6ST 在 C 末端与 8 个氨基酸的 His 标签融合,并通过专有色谱技术进行纯化。

IL6ST Human
Interleukin-6 Signal Transducer Human Recombinant
CYT-1156
ProSpec-IL6ST Human/CYT-1156 1µg
ProSpec-IL6ST Human/CYT-1156 5µg
ProSpec-IL6ST Human/CYT-1156 50µg

Catalogue number

CYT-1156

Synonyms

Interleukin 6 signal transducer, oncostatin M receptor, IL6ST, CD130, CDw130, GP130, GP130-RAPS, IL6R-beta

Introduction

Interleukin-6 Signal Transducer or IL6ST is a receptor, part of the family of class 1 cytokine receptor. IL6ST binds to IL-6 through membrane-anchored or soluble IL-6R starts a connection between another complex of IL6ST and IL-6 , thus forming a homo-dimer and a signal transduction occurs.

Interleukin-6 Signal Transducer 或 IL6ST 是一种受体,是 1 类细胞因子受体家族的一部分。 IL6ST通过膜锚定或可溶性IL-6R与IL-6结合,启动IL6ST和IL-6的另一个复合物之间的连接,从而形成同源二聚体并发生信号转导。

Description

IL6ST Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 605 amino acids and having a molecular mass of 68.9kDa.
IL6ST is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 IL6ST Human 是单条糖基化多肽链,含有 605 个氨基酸,分子量为 68.9kDa。
IL6ST 在 C 末端与 8 个氨基酸的 His 标签融合,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The IL6ST solution contains 10% Glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

ELLDPCGYIS PESPVVQLHS NFTAVCVLKE KCMDYFHVNA NYIVWKTNHF TIPKEQYTII NRTASSVTFT DIASLNIQLT CNILTFGQLE QNVYGITIIS GLPPEKPKNL SCIVNEGKKM RCEWDRGRET HLETNFTLKS EWATHKFADC KAKRDTPTSC TVDYSTVYFV NIEVWVEAEN ALGKVTSDHI NFDPVYKVKP NPPHNLSVIN SEELSSILKL TWTNPSIKSV IILKYNIQYR TKDASTWSQI PPEDTASTRS SFTVQDLKPF TEYVFRIRCM KEDGKGYWSD WSEEASGITY EDRPSKAPSF WYKIDPSHTQ GYRTVQLVWK TLPPFEANGK ILDYEVTLTR WKSHLQNYTV NATKLTVNLT NDRYVATLTV RNLVGKSDAA VLTIPACDFQ ATHPVMDLKA FPKDNMLWVE WTTPRESVKK YILEWCVLSD KAPCITDWQQ EDGTVHRTYL RGNLAESKCY LITVTPVYAD GPGSPESIKA YLKQAPPSKG PTVRTKKVGK NEAVLEWDQL PVDVQNGFIR NYTIFYRTII GNETAVNVDS SHTEYTLSSL TSDTLYMVRM AAYTDEGGKD GPEFTFTTPK FAQGEIELEH HHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-NTRK2 Human/CYT-1159

ProSpec-NTRK2 Human,ProSpec CYT-1159,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines.神经营养受体酪氨酸激酶 2,也称为 NTRK2,是一种酪氨酸蛋白激酶受体,通过调节神经元存活、迁移、增殖、分化和突触形成,参与中枢和外周神经系统的发育和成熟。 可塑性。 NTRK2 通过调节短期突触功能和长期增强作用在学习和记忆中。 TRK 家族受体的已知底物是:SHC1、PI-3 激酶和 PLC-γ-1。

NTRK2 Human
Neurotrophic Receptor Tyrosine Kinase 2 Human Recombinant
CYT-1159
ProSpec-NTRK2 Human/CYT-1159 2µg
ProSpec-NTRK2 Human/CYT-1159 10µg
ProSpec-NTRK2 Human/CYT-1159 1mg

Catalogue number

CYT-1159

Synonyms

GP145-TrkB, trk-B, TRKB, NTRK2

Introduction

Neurotrophic Receptor Tyrosine Kinase 2, also referred to NTRK2, is a tyrosine-protein kinase receptor which takes part in the development & the maturation of the central and the peripheral nervous systems through regulation of neuron survival, migration, proliferation, differentiation and synapse formation & plasticity.  NTRK2 acts in learning and memory by regulating both short term synaptic function and long-term potentiation. The substrates that are known for the TRK family receptors are: SHC1, PI-3 kinase and PLC-gamma-1.

神经营养受体酪氨酸激酶 2,也称为 NTRK2,是一种酪氨酸蛋白激酶受体,通过调节神经元存活、迁移、增殖、分化和突触形成,参与中枢和外周神经系统的发育和成熟。 可塑性。 NTRK2 通过调节短期突触功能和长期增强作用在学习和记忆中。 TRK 家族受体的已知底物是:SHC1、PI-3 激酶和 PLC-γ-1。

Description

NTRK2 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 407 amino acids and having a molecular mass of 45.2kDa.
NTRK2 is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 NTRK2 Human 是一条糖基化多肽链,包含 407 个氨基酸,分子量为 45.2kDa。
NTRK2 在 C 末端与 8 个氨基酸的 His 标签融合,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The NTRK2 solution contains 10% Glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用,请在 4°C 下储存。 储存,在 -20°C 下冷冻更长时间。
对于长期储存,建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

CPTSCKCSAS RIWCSDPSPG IVAFPRLEPN SVDPENITEI FIANQKRLEI INEDDVEAYV GLRNLTIVDS GLKFVAHKAF LKNSNLQHIN FTRNKLTSLS RKHFRHLDLS ELILVGNPFT CSCDIMWIKT LQEAKSSPDT QDLYCLNESS KNIPLANLQI PNCGLPSANL AAPNLTVEEG KSITLSCSVA GDPVPNMYWD VGNLVSKHMN ETSHTQGSLR ITNISSDDSG KQISCVAENL VGEDQDSVNL TVHFAPTITF LESPTSDHHW CIPFTVKGNP KPALQWFYNG AILNESKYIC TKIHVTNHTE YHGCLQLDNP THMNNGDYTL IAKNEYGKDE KQISAHFMGW PGIDDGANPN YPDVIYEDYG TAANDIGDTT NRSNEIPSTD VTDKTGREHL EHHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • NTRK2 Mouse

ProSpec-PECAM1 Human/CYT-924

ProSpec-PECAM1 Human,ProSpec CYT-924,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在 Sf9 杆状病毒中产生的 PECAM1 Human Recombinant 是一个单一的、非糖基化的多肽链,含有 582 个氨基酸,分子量为 65.5kDa。PECAM1 在 C 端与 8 个氨基酸的 His 标签融合,并通过专有色谱技术进行纯化。

PECAM1 Human

Platelet Endothelial Cell Adhesion Molecule 1 Human Recombinant
CYT-924
ProSpec-PECAM1 Human/CYT-924 2µg
ProSpec-PECAM1 Human/CYT-924 10µg
ProSpec-PECAM1 Human/CYT-924 1mg

Catalogue number

CYT-924

Synonyms

Platelet endothelial cell adhesion molecule, PECAM1, CD31, CD31/EndoCAM, endoCAM, GPIIA’, PECA1, PECAM-1, Platelet Endothelial Cell Adhesion Molecule 1.
血小板内皮细胞粘附分子、PECAM1、CD31、CD31/EndoCAM、endoCAM、GPIIA’、PECA1、PECAM-1、血小板内皮细胞粘附分子 1。

Introduction

Platelet endothelial cell adhesion molecule induces susceptibility to atherosclerosis. PECAM1 averts phagocyte ingestion of closely apposed viable cells by transmitting detachment signals, and transforms function upon apoptosis, thus promoting tethering of dying cells to phagocytes. The encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces as a result causing the viable cell’s active repulsion from the phagocyte.
血小板内皮细胞粘附分子诱导动脉粥样硬化易感性。 PECAM1 通过传递分离信号来避免吞噬细胞摄入紧密并列的活细胞,并在细胞凋亡时转变功能,从而促进垂死细胞与吞噬细胞的结合。 通过 PECAM1 在两个细胞表面上的同源性相互作用,活细胞与吞噬细胞相遇,结果导致活细胞主动排斥吞噬细胞。

Description

PECAM1 Human Recombinant produced in Sf9 Baculovirus is a single, non-glycosylated polypeptide chain containing 582 amino acids and having a molecular mass of 65.5kDa .
PECAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒中产生的 PECAM1 Human Recombinant 是一个单一的、非糖基化的多肽链,含有 582 个氨基酸,分子量为 65.5kDa。
PECAM1 在 C 端与 8 个氨基酸的 His 标签融合,并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

PECAM1 protein solution containing Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

QENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQVLV EGVPSPRVTL DKKEAIQGGI VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQVTHLAQEF PEIIIQKDKA IVAHNRHGNK AVYSVMAMVE HSGNYTCKVE SSRISKVSSI VVNITELFSK PELESSFTHL DQGERLNLSC SIPGAPPANF TIQKEDTIVS QTQDFTKIAS KGTYICT AGIDKVVKKS NTVQIVVCEM LSQPRISYDA QFEVIKGQTI EVRCESISGT LPISYQLLKT SKVLENSTKN SNDPAVFKDN PTEDVEYQCV ADNCHSHAKM LSEVLRVKVI APVDEVQISI LSSKVVESGE DIVLQCAVNE GSGPITYKFY REKEGKPFYQ MTSNATQAFW TKQKASKEQE GEYYCTAFNR ANHASSVPRS KILTVRVILA PWKKVEHHHH HH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-TAFA2 Human/CYT-118

ProSpec-TAFA2 Human,ProSpec CYT-118,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Family with sequence similarity 19 -like) member A2, Chemokine-like protein TAFA-2, protein FAM19A2.

TAFA2 Human

Family with Sequence Similarity 19 Member A2 Human Recombinant
CYT-118
ProSpec-TAFA2 Human/CYT-118 5µg
ProSpec-TAFA2 Human/CYT-118 20µg
ProSpec-TAFA2 Human/CYT-118 1mg

Catalogue number

CYT-118

Synonyms

Family with sequence similarity 19 -like) member A2, Chemokine-like protein TAFA-2, protein FAM19A2.

Introduction

TAFA-2 is a 11 kDa secreted protein that belongs to the FAM19/TAFA family of chemokine-like proteins. Similar to other FAM19/TAFA family members, mature TAFA-1 contains 10 regularly spaced cysteine residues with the same pattern: CX7CCX13CXCX14CX11CX4CX5CX10C . Human TAFA-2 is 97% aa identical to mouse TAFA-2 and is expressed in the central nervous system , colon, heart, lung, spleen, kidney, and thymus, however its expression in the CNS is 50 to 1000 fold higher than in other tissues. The biological roles of TAFA family members have not yet been determined.
TAFA-2 是一种 11 kDa 的分泌蛋白,属于趋化因子样蛋白的 FAM19/TAFA 家族。 与其他 FAM19/TAFA 家族成员类似,成熟的 TAFA-1 包含 10 个具有相同模式的规则间隔的半胱氨酸残基:CX7CCX13CXCX14CX11CX4CX5CX10C。 人 TAFA-2 与小鼠 TAFA-2 有 97% 的氨基酸相同,并在中枢神经系统、结肠、心脏、肺、脾、肾和胸腺中表达,但其在中枢神经系统中的表达比在中枢神经系统中高 50 至 1000 倍 其他组织。 TAFA 家族成员的生物学作用尚未确定。

Description

TAFA2 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 101 amino acids and having a molecular mass of 11.2kDa.
The TAFA2 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TAFA2 Human Recombinant 是一种非糖基化的多肽链,含有 101 个氨基酸,分子量为 11.2kDa。
TAFA2 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized TAFA2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 TAFA2 溶解在不低于 100µg/ml 的无菌 18M-cm H2O 中,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TAFA2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TAFA2 should be stored at 4C between 2-7 days and for future use below -18C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TAFA2 虽然在室温下可稳定保存 3 周,但应在 -18C 以下干燥储存。 重组后,TAFA2 应在 4C 下储存 2-7 天,以备将来在 -18C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H

Biological Activity

Fully biologically active when compared to standard. Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.