标签： ProSpec CYT-814

CLI, AAG4, APOJ, KUB1, SGP2, SGP-2, SP-40, TRPM2, TRPM-2, MGC24903, Clusterin, ging-associated gene 4 protein, Apolipoprotein J,Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, CLU.

CLI、AAG4、APOJ、KUB1、SGP2、SGP-2、SP-40、TRPM2、TRPM-2、MGC24903、Clusterin、ging 相关基因 4 蛋白、载脂蛋白 J、补体细胞溶解抑制剂、补体相关蛋白 SP-40、 40，Ku70 结合蛋白 1，NA1/NA2，睾酮抑制的前列腺信息 2，CLU。

Clusterin also named Apolipoprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.
The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices.
Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen.
It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition.
A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates.
Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.

Clusterin 也称为载脂蛋白 J，是一种 75-80 kD 二硫键连接的异二聚体蛋白，含有约 30% 的富含唾液酸的 N 连接碳水化合物，但也已鉴定出靶向细胞核的截短形式。
前体多肽链被蛋白水解切割以去除 22-mer 分泌信号肽，随后在残基 227/228 之间生成 a 和 b 链。这些以反平行方式组装以产生异二聚体分子，其中富含半胱氨酸的中心通过五个二硫键连接，并且两侧是两个预测​​的卷曲螺旋α-螺旋和三个预测的两亲α-螺旋。
在广泛的物种中，clusterin 显示出高度的序列同源性，范围从 70% 到 80%。它几乎在大多数哺乳动物组织中普遍表达，可以在血浆、乳汁、尿液、脑脊液和精液中找到。
它能够与免疫球蛋白、脂质、肝素、细菌、补体成分、对氧磷酶、β淀粉样蛋白、瘦素等众多伙伴结合并形成复合物。凝聚素被赋予了过多的功能，例如吞噬细胞募集、聚集诱导、补体攻击预防、细胞凋亡抑制、膜重塑、脂质转运、激素转运和/或清除、基质金属蛋白酶抑制。
尚未定义 clusterin 的真正功能。一个诱人的假设说，凝聚素是一种细胞外伴侣蛋白，可保护细胞免受由降解和错误折叠的蛋白质沉淀物引起的应激损伤。
在包括癌症、器官再生、感染、阿尔茨海默病、色素性视网膜炎、心肌梗塞、肾小管损伤、自身免疫等在内的许多病理和临床相关情况下，Clusterin 的 mRNA 或蛋白质水平上调或下调。

Clusterin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 463 amino acids and having a molecular mass of 54.1kDa. Clusterin is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Clusterin Human Recombinant 是一种单一的、非糖基化的多肽链，含有 463 个氨基酸，分子量为 54.1kDa。 Clusterin 在 N 末端与 36 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile filtered colorless solution.

Clusterin protein solution contains 20mM Tris-HCl buffer , 0.15M NaCl, 10% glycerol and 1mM DTT.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 85% as determined by -PAGE.

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSDQTV SDNELQEMSN QGSKYVNKEI QNAVNGVKQI KTLIEKTNEE RKTLLSNLEE AKKKKEDALN ETRESETKLK ELPGVCNETM MALWEECKPC LKQTCMKFYA RVCRSGSGLV GRQLEEFLNQ SSPFYFWMNG DRIDSLLEND RQQTHMLDVM QDHFSRASSI IDELFQDRFF TREPQDTYHY LPFSLPHRRP HFFFPKSRIV RSLMPFSPYE PLNFHAMFQP FLEMIHEAQQ AMDIHFHSPA FQHPPTEFIR EGDDDRTVCR EIRHNSTGCL RMKDQCDKCR EILSVDCSTN NPSQAKLRRE LDESLQVAER LTRKYNELLK SYQWKMLNTS SLLEQLNEQF NWVSRLANLT QGEDQYYLRV TTVASHT DVPSGVTEVV VKLFDSDPIT VTVPVEVSRK NPKFMETVAE KALQEYRKKH REE.

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