ProSpec-IFN g Equine/CYT-739

ProSpec-IFN g Equine,ProSpec CYT-739,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

IFN g Equine

Interferon-Gamma Equine Recombinant
CYT-739
5µg
20µg
1mg

Catalogue number

CYT-739

Synonyms

Interferon gamma, IFN-gamma, IFNG.

Introduction

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
由特定抗原或有丝分裂原激活的淋巴细胞产生的 IFN-γ。
IFN-γ除具有抗病毒活性外,还具有重要的免疫调节功能,是巨噬细胞的强效激活剂,对转化细胞具有抗增殖作用,并能增强I型干扰素的抗病毒和抗肿瘤作用。

Description

Recombinant Equine Interferon-gamma produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids and having a molecular mass of 16.7kDa.
The IFN-gamma Equine is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的重组马干扰素-γ 是一种单一的、非糖基化的多肽链,含有 143 个氨基酸,分子量为 16.7kDa。
IFN-γ马通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 5% trehalose.
从 PBS、pH 7.4 和 5% 海藻糖中的 0.2 µm 过滤浓缩溶液冻干。

Solubility

It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中重新配制冻干的干扰素-γ,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的干扰素-γ 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重构后,IFN-γ 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

QAAFFKEIEN LKEYFNASNP DVGDGGPLFL DILKNWKEDS DKKIIQSQIV SFYFKLFENL KDNQVIQKSM DTIKEDLFVK FFNSSTSKLE DFQKLIQIPV NDLKVQRKAI SELIKVMNDL SPKANLRKRK RSQNPFRGRR ALQ.

Biological Activity

The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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