标签： ProSpec CYT-697

AIF-1, Allograft inflammatory factor 1, Em:AF129756.17, G1, IBA1, Ionized calcium-binding adapter molecule 1, IRT-1, Protein G1, AIF1.

Human AIF1 protein shares 98% homology/identity with that of rat. AIF1 is expressed in macrophages and neutrophils. The expression of AIF1 transcripts is upregulated by IFN-g in rat macrophages. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68 macrophages in the interstitial and perivascular spaces of human heart allografts. In quiescent cultured human vascular smooth muscle cells synthesis of AIF1 is induced by IFN-g, IL1b, and conditioned medium of T-cells. Overexpression of AIF1 in human VSMCs results in enhanced growth of these cells. AIF1 is expressed during apoptosis rat mammary gland and ventral prostate tissues. Allograft Inflammatory Factor 1 is expressed by several tumor-associated activated macrophages and microglial cells in rat and human gliomas. There is an evident relationship of AIF1-expressing activated macrophages and microglial cells with tumor malignancy in humans.

人类 AIF1 蛋白与大鼠具有 98% 的同源性/同一性。 AIF1 在巨噬细胞和中性粒细胞中表达。 AIF1 转录物的表达在大鼠巨噬细胞中被 IFN-g 上调。 AIF1 在人类巨噬细胞样细胞系和人类心脏同种异体移植物间质和血管周围空间的 CD68 巨噬细胞亚群中选择性表达。 在静止培养的人血管平滑肌细胞中，AIF1 的合成由 IFN-g、IL1b 和 T 细胞条件培养基诱导。 AIF1 在人类 VSMC 中的过表达导致这些细胞的生长增强。 AIF1 在大鼠乳腺和腹侧前列腺组织细胞凋亡期间表达。 同种异体移植炎症因子 1 由大鼠和人类神经胶质瘤中的几种肿瘤相关活化巨噬细胞和小胶质细胞表达。 表达 AIF1 的活化巨噬细胞和小胶质细胞与人类肿瘤恶性肿瘤有明显的关系。

The AIF1 Human Recombinant contains a total of 155 amino acids having a molecular Mass of 17.7kDa. The Human AIF1 is fused to a 9 amino acid long N-terminal His tag.

AIF1 Human Recombinant 共含有 155 个氨基酸，分子量为 17.7kDa。 人类 AIF1 融合到一个 9 个氨基酸长的 N 端 His 标签。

E. Coli.

Filtered White lyophilized powder.

Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.

Add deionized water and let the lyophilized pellet dissolve completely.

For long term, store lyophilized AIF1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable for 24 months when stored at -20°C.

长期保存冻干的 AIF1 在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。
冻干蛋白在 -20°C 下可保持稳定 24 个月。

MKHHHHHHASQTRDLQGGKAF­GLLKAQQEERLDE­INKQFLDDPKYSSDED­LPSKLEGFKEKYMEF­DLNGNGDIDIMSLKRMLEK­LGVPKTHLELKKLI­GEVSSGSGETFSYP­DFLRMMLGKRSAIL­KMILMYEEKAREKEK­PTGPPAKKAISELP.

Greater than 90% as determined by PAGE.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。