标签： ProSpec CYT-409

Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.

FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on -PAGE at 19kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.

在 Sf9 昆虫细胞中产生的 Flt3-Ligand Human Recombinant 是一种糖基化的多肽链，含有 155 个氨基酸，并在 -PAGE 上以 19kDa 迁移。
Flt3-Ligand 通过专有的色谱技术进行纯化。

Baculovirus Sf9 cells.

Sterile Filtered White lyophilized powder.

The protein was lyophilized with no additives.

It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-L should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt3-L 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.

The ED50, calculated by the dose-dependent stimulation of the proliferation of human MUTZ-2 cells is < 3.0 ng/ml.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard.

蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.42 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. RP-HPLC 分析，使用标准 Flt3-Ligand 作为参考标准。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.