标签： ProSpec CYT-398

Indian hedgehog protein, IHH, HHG-2, BDA1.

IHH belongs to the hedgehog family of secreted signaling molecules. Hedgehog proteins are vital regulators of various developmental processes including growth, patterning and morphogenesis. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog , Indian hedgehog , and Desert hedgehog . IHH has a specific role in bone growth and differentiation. In addition, IHH is involved in yolk sac vasculogenesis, having a central role in differentiation of epiblast cells into endothelial and red blood cells. IHH mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas and strongly in cartilage in growth regions of the developing bone. IHH gene mutations cause the brachydactyly type A1 which is characterized by shortening or malformation of the phalanges and also the acrocapitofemoral dysplasia.

IHH 属于分泌信号分子的刺猬家族。 Hedgehog 蛋白是各种发育过程的重要调节剂，包括生长、模式和形态发生。 Hh 的脊椎动物同源物包括几种蛋白质，包括声波刺猬、印度刺猬和沙漠刺猬。 IHH 在骨骼生长和分化中具有特定的作用。 此外，IHH 参与卵黄囊血管发生，在外胚层细胞分化为内皮细胞和红细胞中起核心作用。 在胎儿肺、肠、胃、肝、肾、胰腺中检测到 IHH mRNA 表达，并且在发育中骨骼的生长区域的软骨中强烈检测到。 IHH 基因突变导致 A1 型短指，其特征是指骨缩短或畸形以及顶股骨发育不良。

IHH Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8kDa.
The IHH is purified by proprietary chromatographic techniques.

在 E.Coli 中产生的 IHH Mouse Recombinant 是一条单一的、非糖基化的多肽链，包含 176 个氨基酸，分子量为 19.8kDa。
IHH 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

It is recommended to reconstitute the lyophilized IHH in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 IHH，然后可以进一步稀释成其他水溶液。

Lyophilized IHH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IHH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 IHH 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，IHH 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG.

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 cells. The expected ED₅₀ for this effect is 3.0-10.0μg/ml corresponding to a specific activity of 100-334units/mg.

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