标签： ProSpec CYT-389

CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.

Endoglin is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex.
The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts, and smooth muscle cells. Endoglin has been found to be part of the TGF-beta1 receptor complex. It thus may be involved in the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beside TGF-beta signaling endoglin may have other functions. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development . Experimental mice without the endoglin gene die due to cardiovascular abnormalities.

Endoglin 是一种位于细胞表面的 I 型膜糖蛋白，是 TGF β 受体复合物的一部分。
该蛋白质由具有二硫键的 180 kDA 同型二聚体组成。 它存在于内皮细胞、活化的巨噬细胞、成纤维细胞和平滑肌细胞上。 已发现内皮糖蛋白是 TGF-β1 受体复合物的一部分。 因此它可能参与 TGF-β1、TGF-β3、激活素 A、BMP-2 和 BMP-7 的结合。 除了 TGF-β 信号转导内皮糖蛋白可能还有其他功能。 据推测，内皮糖蛋白参与影响细胞形态和迁移的细胞骨架组织。 Endoglin 在心血管系统的发育和血管重塑中发挥作用。 它的表达在心脏发育过程中受到调节。 没有内皮糖蛋白基因的实验小鼠因心血管异常而死亡。

CD105 Human Recombinant extracellular domain produced in baculovirus is a homodimeric, glycosylated, Polypeptide containing 586 amino acids and having a molecular mass of 61 kDa but as a result of glycosylation, migrates at 90 kDa under reducing conditions in -PAGE. The CD105 is fused to a C-terminal His-tag and purified by proprietary chromatographic techniques.

杆状病毒中产生的 CD105 人重组细胞外结构域是一种同型二聚体、糖基化多肽，包含 586 个氨基酸，分子量为 61 kDa，但由于糖基化，在 -PAGE 的还原条件下以 90 kDa 迁移。 CD105 与 C 末端 His 标签融合，并通过专有色谱技术进行纯化。

Sf9 Insect Cells.

Sterile Filtered White lyophilized powder.

Endoglin was lyophilized from a concentrated sterile solution containing 1xPBS.

It is recommended to reconstitute the lyophilized CD-105 in sterile PBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 CD-105 溶解在不低于 100µg/ml 的无菌 PBS 中，然后可以进一步稀释成其他水溶液。

Lyophilized Endoglin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD105 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 Endoglin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CD105 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

MDRGTLPLAVALLLASCSLSPTSLAETVHCDLQPVGPERGEVTY TTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVL SVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPI TSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLE GVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLID ANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPL ASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKE LVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNI LSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSE FLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPI PKTGTLSCTVALRPKTGS.

Measured by its ability to bind with rhTGF-beta RII/Fc in a functional ELISA. Optimal dilutions should be determined by each laboratory for each application.

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