标签： ProSpec CYT-331

Fms-Related Tyrosine Kinase 3 Ligand, Flt3 Ligand, Flt-3 Ligand, SL Cytokine.

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.

Flt3-Ligand Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of approximately 17.6kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.

Escherichia Coli.

Sterile Filtered White lyophilized powder.

The protein was lyophilized with no additives.

It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Flt3-L，然后可以进一步稀释成其他水溶液。

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt3-Ligand 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA.

The ED₅₀ as calculated by the dose-dependent stimulation of the proliferation of human AML5 cells is less than 1.0 ng/ml, corresponding to a Specific Activity of 1.0×10⁶ IU/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

1.Title:Identification of Y589 and Y599 in the
juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2.
Publication:Published online before printMay 9, 2006, doi:10.1182/blood-2005-07-008896
Submitted: July 14, 2005Accepted: April 26, 2006 BloodSeptember 1, 2006vol. 108no. 51542-1550.
Link:http://bloodjournal.hematologylibrary.org/content/108/5/1542.full2. Title:A role of Gab2 association in Flt3 ITD mediated Stat5 phosphorylation and cell survival.
Publication:Received 2 February 2009; accepted for publication 30 March 2009 Correspondence: Lars Ro¨nnstrand,Experimental Clinical Chemistry, WallenbergLaboratory, Entr. 46, 5th fl, Malmo¨ University Hospital, SE-20502 Malmo¨, Sweden.E-mail: lars.ronnstrand@med.lu.se;http://www.expklkemi.mas.lu.se
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2141.2009.07725.x/pdf