标签： ProSpec CYT-292

IRF-3, IRF3, IFN Regulatory Factor 3.

Members of the IFN regulatory factor family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection. IRF-3 mediates IFN-stimulated response element promoter activation. Functions as a molecular switch for antiviral activity. Dsrna generated during the course of an viral infection leads to IRF3 phosphorylation on the c-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with creb binding protein to form dsrna-activated factor 1 , a complex which activates the transcription of genes under the control of isre. The complex binds to the ie and prdiii regions on the ifn-alpha and ifn-beta promoters respectively. IRF-3 does not have any transcription activation domains.

IFN 调节因子家族的成员调节对免疫反应、造血和增殖至关重要的基因表达。 IRF-3 是 IRF 家族的成员，与其他家族成员不同。其转录活性仅受翻译后修饰的调节。它在激活先天免疫和响应病毒感染的炎症中起着至关重要的作用。 IRF-3 介导 IFN 刺激的反应元件启动子激活。用作抗病毒活性的分子开关。在病毒感染过程中产生的 Dsrna 导致 C-末端丝氨酸/苏氨酸簇上的 IRF3 磷酸化。这会诱导构象变化，导致其二聚化、核定位并与 creb 结合蛋白结合以形成 dsrna 激活因子 1，这是一种在 isre 控制下激活基因转录的复合物。该复合物分别与 ifn-alpha 和 ifn-beta 启动子上的 ie 和 prdiii 区域结合。 IRF-3 没有任何转录激活域。

IRF-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 13 kDa.
The IRF3 is purified by proprietary chromatographic techniques.

在 E.Coli 中产生的 IRF-3 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 111 个氨基酸，分子量为 13 kDa。
IRF3 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered colorless solution.

1mg/ml in phosphate-buffered saline , pH 7.4.

Liquid IRF3 although stable at 10°C for 1 week, should be stored below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

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ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.