标签： ProSpec CYT-278

CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, Apo-J.

Clusterin also named Apolipoprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.
The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices.
Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen.
It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition.
A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates.
Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.

Clusterin 也称为载脂蛋白 J，是一种 75-80 kD 二硫键连接的异二聚体蛋白，含有约 30% 的富含唾液酸的 N 连接碳水化合物，但也已鉴定出靶向细胞核的截短形式。
前体多肽链被蛋白水解切割以去除 22-mer 分泌信号肽，随后在残基 227/228 之间生成 a 和 b 链。这些以反平行方式组装以产生异二聚体分子，其中富含半胱氨酸的中心通过五个二硫键连接，并且两侧是两个预测​​的卷曲螺旋α-螺旋和三个预测的两亲α-螺旋。
在广泛的物种中，clusterin 显示出高度的序列同源性，范围从 70% 到 80%。它几乎在大多数哺乳动物组织中普遍表达，可以在血浆、乳汁、尿液、脑脊液和精液中找到。
它能够与许多伙伴结合并形成复合物，例如免疫球蛋白、脂质、细菌、补体成分、对氧磷酶、β淀粉样蛋白、瘦素等。凝聚素被赋予了过多的功能，例如吞噬细胞募集、聚集诱导、补体攻击预防、细胞凋亡抑制、膜重塑、脂质转运、激素转运和/或清除、基质金属蛋白酶抑制。
尚未定义 clusterin 的真正功能。一个诱人的假设说，凝聚素是一种细胞外伴侣蛋白，可保护细胞免受由降解和错误折叠的蛋白质沉淀物引起的应激损伤。
在包括癌症、器官再生、感染、阿尔茨海默病、色素性视网膜炎、心肌梗塞、肾小管损伤、自身免疫等在内的许多病理和临床相关情况下，Clusterin 的 mRNA 或蛋白质水平上调或下调。

Clusterin Human Recombinant produced in HEK is a glycosylated, polypeptide chain containing 438 amino acids and having a molecular mass of 51.27 kDa.
Clusterin is fused to 11 a.a. flag tag at c-terminal and purified by proprietary chromatographic techniques.

在 HEK 中产生的 Clusterin Human Recombinant 是一种糖基化的多肽链，包含 438 个氨基酸，分子量为 51.27 kDa。
Clusterin 与 11 a.a. 融合。 在 c 端标记标签并通过专有色谱技术纯化。

293 cell line .

Filtered, White, Lyophilized powder.

Filtered and lyophilized PBS, pH 7.5.

Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

加入去离子水制备约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请使用合适的无菌过滤器过滤产品。

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEAAA DYKDDDDK.

Greater than 95% as determined by PAGE.

1. Tiltle: Clusterin Facilitates Exchange of Glycosyl Phosphatidylinositol-Linked SPAM1 Between Reproductive Luminal Fluids and Mouse and Human Sperm Membranes1.
Publication:BIOLOGY OF REPRODUCTION 81, 562–570 Published online before print 8 April 2009. DOI 10.1095/biolreprod.108.075739
Link:http://www.biolreprod.org/content/81/3/562.full.pdf

2. Tiltle: Mass spectrometry quantification of clusterin in the human brain.
Publication:2012 Chen et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the CreativeCommons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Link:http://link.springer.com/content/pdf/10.1186%2F1750-1326-7-41.pdf

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。