标签： ProSpec CYT-125

Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD95, FAS, APT1, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6.

Fas and Fas Ligand are members of the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas initiates apoptosis in Fas-bearing cells. The apoptosis mechanism involves the recruitment of pro-caspase 8 through an adaptor molecule named FADD followed by processing of the pro-enzyme to active forms. These active caspases subsequently cleave a variety of cellular substrates leading to the eventual cell death. sFasR is able to inhibit FasL-induced apoptosis by acting as a decoy receptor whicht serves as a sink for FasL. The full length Fas Receptor is a 319 a.a type I transmembrane protein, which contains a 157 a.a extracellular domain, a 17 a.a transmembrane domain, and 145 a.a cytoplasmic domain. The mature human Fas ECD shares 55%, 58%, a.a sequence identity with the mouse, rat, Fas, respectively.

Fas 和 Fas 配体是 TNF 超家族的成员，分别是 I 型和 II 型跨膜蛋白。 FasL 与 Fas 的结合启动了携带 Fas 的细胞的凋亡。 细胞凋亡机制涉及通过称为 FADD 的衔接分子募集半胱天冬酶 8 前体，然后将酶原加工成活性形式。 这些活性半胱天冬酶随后切割多种细胞底物，导致最终的细胞死亡。 sFasR 能够通过充当诱饵受体来抑制 FasL 诱导的细胞凋亡，该诱饵受体充当 FasL 的汇。 全长 Fas 受体是一个 319 a.a 的 I 型跨膜蛋白，它包含一个 157 a.a 的胞外结构域、一个 17 a.a 的跨膜结构域和一个 145 a.a 的胞质结构域。 成熟的人 Fas ECD 分别与小鼠、大鼠、Fas 共享 55%、58%、a.a 序列同一性。

sFas Receptor Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 17.6kDa.
The FAS is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 sFas Receptor Human Recombinant 是一条单一的、非糖基化的多肽链，包含 157 个氨基酸，分子量为 17.6kDa。
FAS 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

FAS protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

It is recommended to reconstitute the lyophilized FAS in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 FAS，然后可以进一步稀释成其他水溶液。

Lyophilized FAS although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FAS should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 FAS 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，FAS 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS.

The ED₅₀ was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2ng/ml of hFasL.

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