Acrp30 Mouse
Catalogue number
CYT-1168
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Introduction
Adiponectin, also known as Acrp30, AdipoQ and GBP-28, is a 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . Adiponectin is a part of the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
脂联素又名Acrp30、AdipoQ和GBP-28,是一种244个氨基酸的蛋白质,是apM1基因的产物,具有生理活性,在脂肪细胞中特异性高表达。 脂联素是可溶性防御胶原蛋白超家族的一部分; 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体,它们是血清中循环的高阶复合物的组成部分。
Description
Acrp30 Mouse Recombinant is a single, glycosylated polypeptide chain containing 230 amino acids and having a molecular mass of 24.9kDa
Acrp30 Mouse Recombinant 是单条糖基化多肽链,包含 230 个氨基酸,分子量为 24.9kDa
Source
HEK293 cells.
Physical Appearance
Formulation
Acrp30 filtered and lyophilized from 0.5mg/ml in 20mM Tris 50mM NaCl and 1mM CaCl2, pH 7.5.
Acrp30 在 20mM Tris 50mM NaCl 和 1mM CaCl2,pH 7.5 中从 0.5mg/ml 过滤和冻干。
Solubility
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.
建议加入去离子水配制约 0.5mg/ml 的工作原液,让冻干颗粒完全溶解。
Stability
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间; 在 4°C 下两周后它没有显示任何变化。
Purity
Greater than 90.0% as determined by -PAGE.
Biological Activity
Measured by its ability to inhibit proliferation of mouse 3T3 fibroblasts stimulated by PDGF. The ED50 for the effect is typically 5-15 μg/mL.
Amino acid sequence
EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYVYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN.
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。
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