IFNG Monkey
CYT-1122
ProSpec-IFNG Monkey/CYT-1122 10µg
ProSpec-IFNG Monkey/CYT-1122 50µg
ProSpec-IFNG Monkey/CYT-1122 1mg
Catalogue number
CYT-1122
Synonyms
Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Introduction
IFN-gamma produced by lymphocytes activated by specific antigens / mitogens.
IFN-gamma, on top of having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
由特定抗原/有丝分裂原激活的淋巴细胞产生的 IFN-γ。
IFN-γ除具有抗病毒活性外,还具有重要的免疫调节功能,是巨噬细胞的强效激活剂,对转化细胞具有抗增殖作用,并能增强I型干扰素的抗病毒和抗肿瘤作用。
Description
Interferon-gamma Rhesus Macaque Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 142 amino acid and having a molecular mass of approximately 16.8kDa.
IFNG is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的干扰素-γ恒河猴重组体是一种非糖基化多肽链,含有 142 个氨基酸,分子量约为 16.8kDa。
IFNG 通过专有的色谱技术进行纯化。
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Solubility
It is recommended to reconstitute the lyophilized Interferon-gamma Rhesus Macaque in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中重新配制冻干的干扰素-γ恒河猴,然后可以进一步稀释成其他水溶液。
Stability
Lyophilized IFNG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interferon-gamma Rhesus Macaque should be stored at 4°C between 2-7 days and for future use below -18°C.
冻干的 IFNG 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重组后,γ-干扰素恒河猴应在 4°C 下储存 2-7 天,以备将来在低于 -18°C 的温度下使用。
Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.
Amino acid sequence
QDPYVKEAEN LKKYFNAGDP DVADNGTLFL DILRNWKEES DRKIMQSQIV SFYFKLFKNF KDDQRIQKSV ETIKEDINVK FFNSNKKKRD DFEKLTNYSV TDSNVQRKAV HELIQVMAEL SPAAKIGKRK RSQMFRGRRA SQ.
Biological Activity
The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis virus is < 20.0 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg.
使用感染了脑心肌炎病毒的人 HeLa 细胞通过抗病毒测定确定的 ED50 为 < 20.0 ng/ml,对应于 > 5.0 × 104 IU/mg 的比活性。
Usage
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