APOB Human
Apolipoprotein-B Human
CYT-1042
ProSpec-APOB Human/CYT-1042 100µg
ProSpec-APOB Human/CYT-1042 1mg
ProSpec-APOB Human/CYT-1042 5mg
Catalogue number
CYT-1042
Synonyms
APOB, APO-B, Apolipoprotein B.
Introduction
ApoB is the main apolipoprotein of LDL, IDL, VLDL and chylomicrons particles that serves as the carrier of lipids , as well as cholesterol, in the water surrounding the cells within every tissue across the body. Though all the functional aspects of Apolipoprotein B within the LDL and other elements are considered to be rather uncertain, it serves as the key organizing protein of all other carriers of lipids. across LDL membranes, apolipoprotein B also serves as a ligand for LDL receptors in many cells across the body, meaning, it shows that lipid carriers that are set to cross into cells with Apolipoprotein B receptors, this is the way that lipids are transported within and into cells.
ApoB 是 LDL、IDL、VLDL 和乳糜微粒的主要载脂蛋白，在全身每个组织内的细胞周围的水中充当脂质和胆固醇的载体。 尽管 LDL 和其他元素中载脂蛋白 B 的所有功能方面都被认为是相当不确定的，但它是所有其他脂质载体的关键组织蛋白。 穿过 LDL 膜，载脂蛋白 B 还充当全身许多细胞中 LDL 受体的配体，这意味着，它表明脂质载体将进入带有载脂蛋白 B 受体的细胞，这是脂质在体内和 进入细胞。
Description
Human APOB produced from Human plasma having a molecular mass of 550 kDa.
Source
Human Plasma.
Physical Appearance
Sterile Filtered lyophilized powder.
Formulation
The protein was lyophilized from a 0.5mg/ml solution of 50mM Na2CO3 pH-10 containing 50mM NaCl and 10mM sodium deoxycholate.
从含有 50mM NaCl 和 10mM 脱氧胆酸钠的 50mM Na2CO3 pH-10 的 0.5mg/ml 溶液中冻干蛋白质。
Solubility
It is recommended to reconstitute the lyophilized APOB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干 APOB，然后可以进一步稀释成其他水溶液。
Stability
Lyophilized APOB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, APOB should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage, it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.
冻干的 APOB 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，APOB 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。 请防止冻融循环。
Purity
Greater than 95.0%.
Human Virus Test
Starting material donor has been tested and certified negative for antibodies to HIV-1,HIV-2, HCV, HBSAG.
起始材料供体已经过 HIV-1、HIV-2、HCV、HBSAG 抗体检测并证明为阴性。