标签： ProSpec CYT-1021

Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14.

The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.

TNFRSF12A 基因最初被认为是小鼠 NIH 3T3 成纤维细胞中的一种成纤维细胞生长因子诱导型即刻早期反应基因 Fn14。 人 TNFRSF12A cDNA 编码一个 129 个氨基酸残基的 I 型跨膜蛋白，具有 27 个氨基酸的信号肽、一个 53 个氨基酸的胞外结构域、一个 21 个氨基酸的跨膜结构域和一个 28 个氨基酸的胞质结构域。 人和小鼠 TNFRSF12A 具有 82% 的 aa 序列同一性。 TNFRSF12 是 TNF 受体超家族中最小的成员，在其细胞外结构域中只有一个富含半胱氨酸的区域。 TNFRSF12A 细胞质结构域含有一个结合 TRAF 1、2 和 3 的 TRAF 结合基序。TNFRSF12A 以高亲和力结合其配体 TWEAK/TNFSF12A 以启动信号转导级联反应，该级联反应受细胞类型的影响，导致不同的细胞反应，例如细胞死亡 、细胞增殖和血管生成。

TNFRSF12A Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 292 amino acids and having a molecular mass of 32.6kDa . TNFRSF12A is expressed with a 239 amino acids hIgG- His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF12A 人重组体是单条糖基化多肽链，含有 292 个氨基酸，分子量为 32.6kDa。 TNFRSF12A 在 C 末端用 239 个氨基酸的 hIgG-His 标签表达，并通过专有色谱技术进行纯化。

Sf9, Baculovirus cells.

TNFRSF12A protein solution contains Phosphate Buffered Saline , 1mM DTT and 20% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90% as determined by -PAGE.

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWPLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.