标签： ProSpec CYT-051

Fas ligand , APT1LG1, FASL, TNFSF6, CD178, tumor necrosis factor superfamily member 6, Apoptosis antigen ligand, Fas antigen ligand, APTL, CD95-L.

The type II transmembrane protein FASLG is a member of the tumor necrosis factor superfamily. A fas ligand/receptor interaction has a significant part in the regulation of the immune system and the advancement of cancer. FASLG is expressed on the activated T cell surface as a nondisulfidelinked homotrimer. FASLG binding to Fas/CD95/TNFRSF6 on a nearby cell prompts apoptosis in the Fas expressing cell. FASLG is released from the cell surface by metalloproteinases as a soluble molecule that stays trimeric and is able to bind with Fas, but its capability to activate apoptosis is radically reduced. In addition, FASLG binds to DcR3 – a soluble trap receptor with no signal transduction capabilities. Flawed Fas-mediated apoptosis causes oncogenesis in addition to drug resistance in existing tumors. Constitutive expression of FASLG in a variety of tumors enables their immune evasion. Both mouse and human FASLG are active on mouse and human cells.

II型跨膜蛋白FASLG是肿瘤坏死因子超家族的成员。 fas 配体/受体相互作用在免疫系统调节和癌症进展中具有重要作用。 FASLG 在活化的 T 细胞表面以非二硫键同源三聚体的形式表达。 FASLG 与附近细胞上的 Fas/CD95/TNFRSF6 结合会促使表达 Fas 的细胞发生凋亡。 FASLG 作为可溶性分子通过金属蛋白酶从细胞表面释放，保持三聚体并能够与 Fas 结合，但其激活细胞凋亡的能力从根本上降低。 此外，FASLG 与 DcR3 结合 – 一种没有信号转导能力的可溶性陷阱受体。 除了现有肿瘤的耐药性外，有缺陷的 Fas 介导的细胞凋亡还会导致肿瘤发生。 FASLG 在多种肿瘤中的组成型表达使其能够逃避免疫。 小鼠和人类 FASLG 都对小鼠和人类细胞具有活性。

Recombinant Human FAS Ligand produced in HEK293 cells is a polypeptide chain containing 147 amino acids .
FASLG is fused to a 6 amino acid His-tag at N-terminus and purified by proprietary chromatographic techniques.

HEK293细胞产生的重组人FAS配体是一条含有147个氨基酸的多肽链。
FASLG 在 N 末端与 6 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

HEK293 cells.

Sterile Filtered colorless solution.

The FASLG solution contains 1xPBS.

FASLG Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.

FASLG Human Recombinant 虽然在 4°C 下稳定 1 周，但应在 -18°C 以下储存。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by SEC-HPLC.
Analysis by -PAGE.

Fas ligand is biologically active as determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The expected ED₅₀< 10 ng/ml, corresponding to a specific activity of 1×10⁵ units/mg.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.