标签： ProSpec CYT-043

Tumor necrosis factor receptor superfamily member 12A, FN14, CD266 antigen, TweakR, tweak-receptor, Fibroblast growth factor-inducible immediate-early response protein 14, FGF-inducible 14, type I transmembrane protein Fn14.

The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.

TNFRSF12A 基因最初被认为是小鼠 NIH 3T3 成纤维细胞中的一种成纤维细胞生长因子诱导型即刻早期反应基因 Fn14。 人 TNFRSF12A cDNA 编码一个 129 个氨基酸残基的 I 型跨膜蛋白，具有 27 个氨基酸的信号肽、一个 53 个氨基酸的胞外结构域、一个 21 个氨基酸的跨膜结构域和一个 28 个氨基酸的胞质结构域。 人和小鼠 TNFRSF12A 具有 82% 的 aa 序列同一性。 TNFRSF12 是 TNF 受体超家族中最小的成员，在其细胞外结构域中只有一个富含半胱氨酸的区域。 TNFRSF12A 细胞质结构域含有一个结合 TRAF 1、2 和 3 的 TRAF 结合基序。TNFRSF12A 以高亲和力结合其配体 TWEAK/TNFSF12A 以启动信号转导级联反应，该级联反应受细胞类型的影响，导致不同的细胞反应，例如细胞死亡 、细胞增殖和血管生成。

TNFRSF12A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 5.6 KDa.
The TNFRSF12A is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFRSF12A 人重组体是单条非糖基化多肽链，含有 53 个氨基酸，分子量为 5.6 KDa。
TNFRSF12A 通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

It is recommended to reconstitute the lyophilized TNFRSF12A in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 TNFRSF12A 溶解在不低于 100µg/ml 的无菌 18M-cm H2O 中，然后可以进一步稀释成其他水溶液。

Lyophilized TNFRSF12A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF12A should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFRSF12A 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后 TNFRSF12A 应在 4°C 下储存 2-7 天，以备将来在低于 -18°C 的温度下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP.

The TNFRSF12A biological activity is determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED50 for this effect is 1.0-5.0ug/ml in the presence of 1ug/ml rhTWEAK.

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