BMP 2 Protein Human
Bone Morphogenetic Protein-2 Human Recombinant
CYT-261
ProSpec-BMP 2 Protein Human/CYT-261 2µg
ProSpec-BMP 2 Protein Human/CYT-261 10µg
ProSpec-BMP 2 Protein Human/CYT-261 1mg
Catalogue number
CYT-261
Synonyms
BMP-2, BMP2A.
Introduction
BMP2 belongs to the transforming growth factor-beta superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia ossificans progressiva.
BMP2属于转化生长因子-β超家族。 骨形态发生蛋白诱导骨形成。 BMP2 是进行性骨化纤维发育不良的常染色体显性遗传病的候选基因。
Description
Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 2×115 amino acids and having a molecular mass of 26kDa.
The BMP-2 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Bone Morphogenetic Protein-2 Human Recombinant 是一种同源二聚体、非糖基化多肽链，含有 2×115 个氨基酸，分子量为 26kDa。
BMP-2 通过专有的色谱技术进行纯化。
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
BMP2 was lyophilized from a concentrated sterile solution containing 10mM sodium citrate pH=3.5.
Solubility
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 Bone Morphogenetic Protein-2 溶解在不低于 100µg/ml 的无菌 20mM AcOH 中，然后可以进一步稀释成其他水溶液。
Stability
Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干骨形态发生蛋白 2 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，BMP2 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。
Purity
Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.
Amino acid sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR.
Biological Activity
The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 µg/ml.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.4 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. RP-HPLC 分析，使用 BMP-2 标准溶液作为参考标准。
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。
References
Title:β-Catenin Signaling Pathway Is Crucial for Bone Morphogenetic Protein 2 to Induce New Bone Formation.
Publication:First Published on November 3, 2006, doi: 10.1074/jbc.M602700200
January 5, 2007 The Journal of Biological Chemistry, 282, 526-533.
Link:http://www.jbc.org/content/282/1/526.full
