标签： ProSpec Apo D Human

Apolipoprotein D, Apo-D, ApoD.

Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid.
Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimer’s disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue.

载脂蛋白-D主要与人血浆中的高密度脂蛋白有关。载脂蛋白-D 是一种非典型载脂蛋白，根据其一级结构，载脂蛋白-D 是载脂蛋白家族的成员。脂质运载蛋白采用β-桶三级结构并运输小的疏水配体。载脂蛋白-D 结合胆固醇、孕酮、孕烯醇酮、胆红素和花生四烯酸。
载脂蛋白-D在脾脏、睾丸和脑中具有高水平表达的许多组织中表达。载脂蛋白-D 以高浓度存在于患有严重乳腺囊性疾病的女性的囊液中，这种疾病与乳腺癌风险增加有关。载脂蛋白-D 积聚在再生的周围神经和患有神经退行性疾病（如阿尔茨海默病）患者的脑脊液中。载脂蛋白-D 参与中枢和外周神经系统的维护和修复。载脂蛋白-D 是一种多配体、多功能转运蛋白，可将配体从一个细胞转运到器官内的另一个细胞，清除器官内的配体以转运到血液中，或者可以将配体从循环转运到器官内的特定细胞组织。

Apolipoprotein-D Human Recombinant His Tag fusion protein at C-terminus produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.82kDa.
The protein a.a sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.
The Following gene modifications were made:
Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apolipoprotein-D to enhance the protein’s solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchanges which facilitate its genetic manipulation. The Apolipoprotein-D is purified by proprietary chromatographic techniques.

载脂蛋白-D 人重组 His Tag 融合蛋白在 C 端产生于大肠杆菌中，是一种单一的、非糖基化的多肽链，含有 174 个氨基酸，分子量为 19.82kDa。
蛋白质 a.a 序列对应于 UniProtKB/Swiss-Prot 条目 P05090。
进行了以下基因修饰：
在载脂蛋白-D 表面引入 Trp99His、Cys116Ser、Ile118Ser、Leu120Ser 氨基酸交换以增强蛋白质的溶解度，另外三个 Leu23Pro、Pro133Val、Asn134Ala 氨基酸交换有助于其遗传操作。 载脂蛋白-D 是通过专有的色谱技术纯化的。

Escherichia Coli.

Filtered White lyophilized powder.

Filtered and lyophilized from 1mg/ml in 4mM KH₂PO₄, 16mM Na₂HPO₄ and 115mM NaCl pH 7.5.

It is recommended to add deionized H₂O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by an appropriate sterile filter before using it in the cell culture.

建议加入去离子水至 0.5mg/ml 的工作体积，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤本产品。

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Greater than 95% as determined by -PAGE.

FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。