标签： ProSpec ANGPTL3 Human

Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.

ANGPTL3 and ANGPTL4 are angiopoietin-like proteins secreted and expressed mainly by the liver, their role being the regulation of triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. During different nutritional states the levels of the circulating triglycerides are regulated by Angptl3 and Angptl4 through differential inhibition of Lipoprotein lipase as shown by the experimental data. The molecular structure of ANGPTL3 is similar to that of the angiopoietins . Deletion mutants of human Angiopoietin 5 were used in order to demonstrate that the N-terminal domain and not the C-terminal fibrinogen-like domain increased the plasma triglyceride levels in mice.

ANGPTL3和ANGPTL4是主要由肝脏分泌和表达的血管生成素样蛋白，它们的作用是通过抑制富含甘油三酯的脂蛋白的脂解来调节甘油三酯代谢。 如实验数据所示，在不同的营养状态期间，循环甘油三酯的水平由 Angptl3 和 Angptl4 通过对脂蛋白脂肪酶的差异抑制来调节。 ANGPTL3 的分子结构与血管生成素相似。 使用人血管生成素 5 的缺失突变体来证明 N 端结构域而不是 C 端纤维蛋白原样结构域增加了小鼠血浆甘油三酯水平。

The ANGPTL3 Human Recombinant is produced with N-terminal fusion of His-Tag. The Angiopoietin-like protein 3 His Tagged Fusion Protein is 26kDa containing 207 amino acid residues of the ANGPTL3 Human and 16 additional amino acid residues – His-Tag .

ANGPTL3 Human Recombinant 是由 His-Tag 的 N 端融合产生的。 血管生成素样蛋白 3 His 标签的融合蛋白是 26kDa，含有 ANGPTL3 Human 的 207 个氨基酸残基和 16 个额外的氨基酸残基 – His-Tag。

Escherichia Coli.

ANGPTL3 Human filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Angiopoietin 5 purity is greater than 95% as determined by -PAGE.

Add 0.1M Acetate buffer pH-4 and let the lyophilized pellet of ANGPTL3 Human dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of Angiopoietin 5 is limited.

加入 0.1M 醋酸缓冲液 pH-4，让 ANGPTL3 Human 冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10μg/ml 的浓度。 在较高浓度下，血管生成素 5 的溶解度是有限的。

Store lyophilized ANGPTL3 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Angiopoietin 5 can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP.

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