标签： ProSpec ANGPT1 Human

Angiopoietin 1, KIAA0003, ANG-1, AGP1, AGPT.

ANGPT1 is an angiogenic factor which intervenes in blood vessel maturation and takes part in endothelial development. ANGPT1 is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. The glycosylated ANGPT1 protein has a coiled-coil region in the amino terminus and a fibrinogen-like domain at the carboxy terminus.

ANGPT1是一种血管生成因子，可干预血管成熟并参与内皮发育。 ANGPT1 是 Tie-2 的分泌配体，Tie-2 是一种在内皮细胞和造血细胞中表达的细胞表面受体酪氨酸激酶。 糖基化的 ANGPT1 蛋白在氨基末端有一个卷曲螺旋区，在羧基末端有一个纤维蛋白原样结构域。

ANGPT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 480 amino acids and having a molecular mass of 55.6 kDa.
The ANGPT1 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 ANGPT1 Human Recombinant 是单条非糖基化多肽链，包含 480 个氨基酸，分子量为 55.6 kDa。
ANGPT1 通过专有的色谱技术进行纯化。

E.coli.

Sterile Filtered colorless solution.

The ANGPT1 solution contains 20mM Tris-HCl buffer , 1M Urea and 5% glycerol.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 85% as determined by -PAGE.

MSNQRRSPEN SGRRYNRIQH GQCAYTFILP EHDGNCREST TDQYNTNALQ RDAPHVEPDF SSQKLQHLEH VMENYTQWLQ KLENYIVENM KSEMAQIQQN AVQNHTATML EIGTSLLSQT AEQTRKLTDV ETQVLNQTSR LEIQLLENSL STYKLEKQLL QQTNEILKIH EKNSLLEHKI LEMEGKHKEE LDTLKEEKEN LQGLVTRQTY IIQELEKQLN RATTNNSVLQ KQQLELMDTV HNLVNLCTKE GVLLKGGKRE EEKPFRDCAD VYQAGFNKSG IYTIYINNMP EPKKVFCNMD VNGGGWTVIQ HREDGSLDFQ RGWKEYKMGF GNPSGEYWLG NEFIFAITSQ RQYMLRIELM DWEGNRAYSQ YDRFHIGNEK QNYRLYLKGH TGTAGKQSSL ILHGADFSTK DADNDNCMCK CALMLTGGWW FDACGPSNLN GMFYTAGQNH GKLNGIKWHY FKGPSYSLRS TTMMIRPLDF

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

This factor was found in the conditioned medium of the human neuroepithelioma cell line SHEP1 and the mouse myoblast cell line C2C12ras. The cDNA encoding a protein of 498 amino acids was isolated by using a secretion-trap expression cloning procedure exploiting the presence of a signal sequence present in growth factors that are secreted by producer cells . Murine and human factors show 97 % identity at the amino acid level. For a related factor see: CDT6. The human gene has been mapped to chromosome 8q22.3-q23 .

The expression of angiopoietin-1 mRNA is downregulated by PDGF, EGF, IL1-beta, and TGF-beta .

Angiopoietin-1 is a ligand for the receptor-like tyrosine kinase designated TIE-2 . Binding of Angiopoietin-1 to its receptor induces tyrosine phosphorylation of the cytoplasmic receptor domain. A naturally occuring antagonist of Angiopoietin-1 binding to TIE-2 is Angiopoietin-2. Angiopoietin-1 also binds to the TIE-1 receptor and this interaction appears to be critical for the development of the right-hand side venous system. It is dispensable for the left-hand side venous system, suggesting that right-hand and left-hand side vascular networks are established early before asymmetrical features of the network become morphologically discernible Loughna and Sato, 2001).

Angiopoietin-1 does not directly promote the growth of cultured endothelial cells. Angiopoietin-1 is chemotactic for endothelial cells . Excess soluble TIE-2 receptors abolish the chemotactic response of endothelial cells toward angiopoietin-1. Angiopoietin-1 has been shown to counteract cell death by apoptosis in cultured endothelial cells . Angiopoietin-1 also acts as an apoptosis survival factor for endothelial cells and this effect is augmented by the presence of VEGF .

该因子存在于人神经上皮瘤细胞系 SHEP1 和小鼠成肌细胞系 C2C12ras 的条件培养基中。编码 498 个氨基酸的蛋白质的 cDNA 是通过使用分泌陷阱表达克隆程序分离的，该程序利用存在于由生产细胞分泌的生长因子中存在的信号序列。鼠科动物和人类因素在氨基酸水平上显示出 97% 的同一性。有关相关因素，请参阅：CDT6。人类基因已被映射到染色体 8q22.3-q23 。

血管生成素-1 mRNA 的表达被 PDGF、EGF、IL1-β 和 TGF-β 下调。

Angiopoietin-1 是受体样酪氨酸激酶的配体，称为 TIE-2。 Angiopoietin-1 与其受体的结合诱导细胞质受体结构域的酪氨酸磷酸化。血管生成素-1 与 TIE-2 结合的天然拮抗剂是血管生成素-2。 Angiopoietin-1 也与 TIE-1 受体结合，这种相互作用似乎对右侧静脉系统的发育至关重要。它对于左侧静脉系统是可有可无的，这表明右侧和左侧血管网络在网络的不对称特征成为形态学上可辨别的早期建立之前 Loughna 和 Sato，2001）。

Angiopoietin-1 不直接促进培养的内皮细胞的生长。 Angiopoietin-1 对内皮细胞具有趋化作用。过量的可溶性 TIE-2 受体消除了内皮细胞对血管生成素-1 的趋化反应。 Angiopoietin-1 已被证明可通过培养的内皮细胞中的细胞凋亡来抵消细胞死亡。 Angiopoietin-1 还充当内皮细胞的凋亡存活因子，并且这种作用因 VEGF 的存在而增强。

Angiopoietin-2 dose-dependently blocks directed migration toward Angiopoietin-1. Carlson et al have shown that Ang-1 binds rather selectively to vitronectin and that Ang-1 can directly support adhesion of human umbilical vein endothelial cells and fibroblasts in a process mediated by integrins.

The physiologic roles of Angiopoietin-1 and its receptor are limited to angiogenic processes that occur subsequent to the earlier vasculogenic and angiogenic actions of the VEGF family and their receptors. However, it is unlike most of the known angiogenesis factors such as VEGF and other classical endothelial cell growth factors in that addition of the factor to cultures of endothelial cells does not directly promote cell growth even though the TIE-2 receptor becomes activated. Angiopoietin-1 also appears to be incapable of inducing the formation of tubules by endothelial cells.

Angiopoietins can potentiate the effects of other angiogenic cytokines. An investigation of the impact of angiopoietins on neovascularization in vivo in the cornea micropocket assay of neovascularization demonstrates that neither Angiopoietin-1 nor Angiopoietin-2 alone promote neovascularization. Holash et al have shown that a subset of tumors initially grows by coopting existing host vessels. Regression of these vessels via a process that involves disruption of interactions between endothelial cells and smooth muscle cells as well as cell death by apoptosis of endothelial cells first causes loss of tumour cells before angiogenesis begins at the tumor margin and the tumor is rescued under the influence of VEGF, Angiopoietin-1, and probably other angiogenic stimuli.

The embryonic expression pattern of Angiopoietin-1 suggests that it plays a particularly important role in the developing heart. Angiopoietin-1 is expressed highly in the myocardial wall surrounding the endocardium expressing TIE-2. Expression of Angiopoietin-1 becomes much more widespread later in development.

血管生成素可以增强其他血管生成细胞因子的作用。在新血管形成的角膜微袋试验中对血管生成素对体内新血管形成的影响的研究表明，Angiopoietin-1 和 Angiopoietin-2 都不能单独促进新血管形成。 Holash 等人已经表明，一部分肿瘤最初是通过吸收现有的宿主血管来生长的。通过涉及破坏内皮细胞和平滑肌细胞之间的相互作用以及内皮细胞凋亡引起的细胞死亡的过程使这些血管退化，首先导致肿瘤细胞在肿瘤边缘开始血管生成之前损失，并且在影响下挽救肿瘤VEGF、血管生成素-1 和可能的其他血管生成刺激。

Angiopoietin-1 的胚胎表达模式表明它在发育中的心脏中起着特别重要的作用。血管生成素-1 在表达 TIE-2 的心内膜周围的心肌壁中高度表达。 Angiopoietin-1 的表达在发育后期变得更加普遍。