标签： ProSpec-Acrp30 Human HEK

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

脂联素是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag.

脂联素是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

HEK293 .

In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED50 was ~6 µg/ml.

在原代肝细胞中进行了体外糖异生测定，表明源自哺乳动物细胞的人脂联素可以抑制葡萄糖的产生。 ED50 为~6 µg/ml。

White lyophilized powder.

Filtered and lyophilized from 0.5mg/ml in 50mM phosphate Buffer, 75mM NaCl, pH 7.4.

Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

加入去离子水制备约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请使用合适的无菌过滤器过滤产品。

For long term, store lyophilized AdipoQ at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable for 24 months when stored at -20°C.

长期保存冻干的 AdipoQ 于 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。 冻干蛋白在 -20°C 下可保持稳定 24 个月。

Greater than 98% as determined by HPLC and PAGE.

ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。