标签： ProSpec产品

ProSpec-RELM a Mouse, His/CYT-453

ProSpec-RELM a Mouse His,ProSpec CYT-453,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,RELM-alpha Mouse Recombinant 是用噬菌体 fd 的信号序列和 flagTag 的 C 端融合物制造的。 RELM-alpha Flag-Tagged 融合蛋白是一种 13.3 kDa 的蛋白质，含有 91 个氨基酸残基和 30 个额外的氨基酸残基——噬菌体 fd 的信号序列 flagTag 。

RELM a Mouse, His
RELM-Alpha Mouse Recombinant, His Tag
CYT-453
5µg
25µg
1mg

Catalogue number

CYT-453

Synonyms

Resistin-like alpha, RELMalpha, Cysteine-rich secreted protein FIZZ1, Parasite-induced macrophage novel gene 1 protein, Cysteine-rich secreted protein A12-gamma, RELM-a.

Introduction

Bronchoalveolar lavage fluid from mice with experimentally induced allergic pulmonary inflammation contains a novel 9.4 kDa cysteine-rich secreted protein, RELM-alpha . RELM-alpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue stroma. Murine RELM-alpha is the founding member of a new gene family including two other murine genes expressed, respectively, in intestinal crypt epithelium and white adipose tissue , and two related human genes.
RELMalpha inhibits the differentiation of 3T3-L1 preadipocytes into adipocytes but has no effect on proliferation of 3T3-L1 preadipocytes. RELMalpha is able to form heterooligomers with resistin but not RELMbeta. Since RELMalpha is expressed by adipose tissue and it is a secreted factor, our findings suggest that RELMalpha may be involved in the control of the adipogenesis as well as in the process of muscle differentiation.
In the lung, RELM-alpha is induced by hypoxia and was renamed as hypoxia-induced mitogenic factor . HIMF strongly activated Akt phosphorylation. The phosphatidylinositol 3-kinase inhibitor LY294002 inhibited HIMF-activated Akt phosphorylation. It also inhibited HIMFstimulated RPSM proliferation. Thus, the PI3K/Akt pathway, at least in part, mediates the proliferative effect of HIMF. Further studies showed that HIMF had angiogenic and vasoconstrictive properties. HIMF increased pulmonary arterial pressure and vascular resistance. Further studies suggest that HIMF regulates apoptosis and may participate in lung alveolarization and maturation.
来自患有实验性过敏性肺部炎症的小鼠的支气管肺泡灌洗液含有一种新型的 9.4 kDa 富含半胱氨酸的分泌蛋白 RELM-α。 RELM-α 是一种分泌蛋白，其组织分布受限，在脂肪组织基质中含量最高。鼠类 RELM-α 是一个新基因家族的创始成员，该家族包括另外两个分别在肠隐窝上皮和白色脂肪组织中表达的鼠类基因，以及两个相关的人类基因。
RELMalpha 抑制 3T3-L1 前脂肪细胞分化为脂肪细胞，但对 3T3-L1 前脂肪细胞的增殖没有影响。 RELMalpha 能够与抵抗素形成异源寡聚体，但不能与 RELMbeta 形成。由于 RELMalpha 由脂肪组织表达并且它是一种分泌因子，我们的研究结果表明 RELMalpha 可能参与控制脂肪生成以及肌肉分化过程。
在肺中，RELM-α是由缺氧诱导的，并被重新命名为缺氧诱导的有丝分裂因子。 HIMF 强烈激活 Akt 磷酸化。磷脂酰肌醇 3-激酶抑制剂 LY294002 抑制 HIMF 激活的 Akt 磷酸化。它还抑制 HIMF 刺激的 RPSM 增殖。因此，PI3K/Akt 通路至少部分介导了 HIMF 的增殖作用。进一步的研究表明，HIMF 具有血管生成和血管收缩特性。 HIMF 增加肺动脉压和血管阻力。进一步的研究表明，HIMF 调节细胞凋亡并可能参与肺泡化和成熟。

Description

RELM-alpha Mouse Recombinant is manufactured with a signal sequence of phage fd and C-terminal fusion of flagTag . The RELM-alpha Flag-Tagged Fusion Protein is a 13.3 kDa protein containing 91 amino acid residues with 30 additional amino acid residues – signal sequence of phage fd, flagTag .
RELM-alpha Mouse Recombinant 是用噬菌体 fd 的信号序列和 flagTag 的 C 端融合物制造的。 RELM-alpha Flag-Tagged 融合蛋白是一种 13.3 kDa 的蛋白质，含有 91 个氨基酸残基和 30 个额外的氨基酸残基——噬菌体 fd 的信号序列 flagTag 。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5 mg/ml in 5mM Tris pH 7.5, 25mM NaCl.

Solubility

Add deionized H2O and let the lyophilized pellet dissolve completely.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MKKLLFAIPL VVPFYSHSTM VNTDETIEII VENKVKELLA NPANYPSTVT TLSCTSVKT MNRWASCPAG MTATGCACGF ACGSWEIQSG DTCNCLCLLV DWTTARCCQL SLEDYKDDDD K.

Purity

Greater than 95% as determined by -PAGE.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • RELM a Mouse

ProSpec-RELM b Human/CYT-780

ProSpec-RELM b Human,ProSpec CYT-780,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.

RELM b Human
RELM-Beta Human Recombinant
CYT-780
5µg
25µg
1mg

Catalogue number

CYT-780

Synonyms

Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.

Introduction

RELM-beta is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin , RELM-alfa , RELM-beta and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents.
RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant.
The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
RELM-beta 是最近发现的一个分泌蛋白家族的成员，该家族含有一个保守的富含半胱氨酸的 C 末端。 RELM 家族由抵抗素、RELM-α、RELM-β 和 RELM-γ 组成。 在人类中仅发现了抵抗素和 RELM-beta，而在啮齿动物中发现了所有四个 RELM 家族成员。
RELM-beta 似乎仅由肠杯状细胞以同源二聚体形式产生，并且可以在粪便中大量发现。 值得注意的是，在无菌小鼠进入自然环境后发生细菌定植之前，展示无菌肠道的无菌小鼠粪便中不含 RELM-β。 一些（但不是全部）结肠癌细胞系将 RELM-β 分泌到细胞培养上清液中。
RELM-beta 的生理功能尚不清楚。 高剂量的重组 RELM-beta 显示出高血糖作用，包括降低小鼠的葡萄糖处理和增加肝葡萄糖的产生。

Description

RELM-b Human Recombinant produced in E.Coli is a disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 x 89 amino acids and having a total molecular mass of 19kDa.
RELM-b is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 RELM-b Human Recombinant 是一种二硫键连接的同型二聚体、非糖基化多肽链，含有 2 x 89 个氨基酸，总分子量为 19kDa。
RELM-b 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

RELM-b was lyophilized from a solution containing 0.1% Trifluoroacetic Acid .

Solubility

It is recommended to reconstitute the lyophilized RELM-b in sterile 0.1% TFA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized RELM-b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RELM-b Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

Amino acid sequence

MQCSLDSVMD KKIKDVLNSL EYSPSPISKK LSCASVKSQG RPSSCPAGMA VTGCACGYGC GSWDVQLETT CHCQCSVVDW TTARCCHLT.

Purity

Greater than 90.0% as determined by -PAGE.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • RELM b Mouse
  • RELM b Human, His

ProSpec-RELM b Mouse/CYT-413

ProSpec-RELM b Mouse,ProSpec CYT-413,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.

RELM b Mouse
RELM-Beta Mouse Recombinant
CYT-413
ProSpec-RELM b Mouse/CYT-413 5µg
ProSpec-RELM b Mouse/CYT-413 25µg
ProSpec-RELM b Mouse/CYT-413 1mg

Catalogue number

CYT-413

Synonyms

Resistin-like beta, RELM beta, Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2.

Introduction

RELM-beta is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin , RELM-alfa , RELM-beta and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents.
RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant.
The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
RELM-beta 是最近发现的一个分泌蛋白家族的成员，该家族含有一个保守的富含半胱氨酸的 C 末端。 RELM 家族由抵抗素、RELM-α、RELM-β 和 RELM-γ 组成。 在人类中仅发现了抵抗素和 RELM-beta，而在啮齿动物中发现了所有四个 RELM 家族成员。
RELM-beta 似乎仅由肠杯状细胞以同源二聚体形式产生，并且可以在粪便中大量发现。 值得注意的是，在无菌小鼠进入自然环境后发生细菌定植之前，展示无菌肠道的无菌小鼠粪便中不含 RELM-β。 一些（但不是全部）结肠癌细胞系将 RELM-β 分泌到细胞培养上清液中。
RELM-beta 的生理功能尚不清楚。 高剂量的重组 RELM-beta 显示出高血糖作用，包括降低小鼠的葡萄糖处理和增加肝葡萄糖的产生。

Description

Mouse RELM-b Recombinant produced in E.Coli is a monomeric, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 8.9kDa.
The Mouse RETNLB is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的小鼠 RELM-b 重组体是一种单体的、非糖基化的多肽链，含有 83 个氨基酸，分子量为 8.9kDa。
Mouse RETNLB 是通过专有的色谱技术纯化的。

Source

Escherichia Coli.

Physical Appearance

Brownish lyophilized powder.

Formulation

The protein was lyophilized from a concentrated protein solution containing 10mM Acetic Acid with 2:1 mannitol to protein.

Solubility

Reconstitute at 0.1 mg/ml with sterile pyrogen free water.

Stability

Lyophilized RETNLB is stable at -20°C. After reconstitution the protein should be kept at all times at -20°C. It is recommended to add a carrier protein for long term storage.
冻干的RETNLB在-20°C下是稳定的。 重组后的蛋白质应始终保持在-20°C。 建议添加载体蛋白以进行长期储存。

Purity

Greater than 97% as determined by -PAGE.

Amino acid sequence

MQCSFESLVD QRIKEALSRQ EPKTISCTSV TSSGRLASCP AGMVVTGCAC GYGCGSWDIR NGNTCHCQCS VMDWASARCC RMA.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • RELM b Human, His
  • RELM b Human

ProSpec-RELM g Mouse/CYT-1035

ProSpec-RELM g Mouse,ProSpec CYT-1035,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,建议在无菌 18MΩ-cm H2O 中以 100µg/ml 的浓度重新配制冻干的 RELM g，然后可以将其进一步稀释成其他水溶液。

RELM g Mouse
RELM-Gamma Mouse Recombinant
CYT-1035
5µg
25µg
1mg

Catalogue number

CYT-1035

Synonyms

Resistin-like gamma, RELMgamma,RELM-γ, RELM-g.

Introduction

RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone family in mice and rats. Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.

RELM-γ是抵抗素样分子的新成员/发现于小鼠和大鼠的炎症区家族。 微阵列和实时 RT-PCR 实验揭示了香烟烟雾暴露与未处理大鼠的鼻呼吸道上皮细胞中 RELMgamma mRNA 的抑制。 生理组织特异性表达的分析揭示了造血组织中的最高表达，表明 RELM-γ 具有细胞因子样作用。 在骨髓、脾脏和肺以及外周血粒细胞中可检测到 RELM-γ-mRNA。 与模拟转染的细胞相比，用 RELM-γ 表达质粒转染的早幼粒细胞 HL60 细胞具有增加的增殖率，并显示出对视黄酸诱导的粒细胞分化的反应改变。 总之，这些数据提供了第一个实验证据，证明 RELM-γ 是一种具有受限表达模式的分泌分子，可能在早幼粒细胞分化中起作用。

Description

RELM g Mouse Recombinant produced in E.Coli is a non glycosylated, homodimeric polypeptide chain containing 2 x 89 amino acids and having a total molecular mass of 18.9kDa.

The RELM is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 RELM g 小鼠重组体是一种非糖基化的同型二聚体多肽链，含有 2 x 89 个氨基酸，总分子量为 18.9kDa。

RELM 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid .

Solubility

It is recommended to reconstitute the lyophilized RELM g in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.建议在无菌 18MΩ-cm H2O 中以 100µg/ml 的浓度重新配制冻干的 RELM g，然后可以将其进一步稀释成其他水溶液。

Stability

Lyophilized RELM g although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RELM g Mouse should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MEGTLESIVE KKVKELLANR DDCPSTVTKT FSCTSITASG RLASCPSGMT VTGCACGYGC GSWDIRDGNT CHCQCSTMDW ATARCCQLA.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • RELM g Mouse, His

ProSpec-RELM g Mouse, His/CYT-455

ProSpec-RELM g Mouse, His,ProSpec CYT-455,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

RELM g Mouse, His
RELM-Gamma Mouse Recombinant, His Tag
CYT-455
5µg
25µg
1mg

Catalogue number

CYT-455

Synonyms

Resistin-like gamma, RELMgamma,RELM-γ, RELM-g.

Introduction

RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone family in mice and rats. Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.
RELM-γ是抵抗素样分子的新成员/发现于小鼠和大鼠的炎症区家族。 微阵列和实时 RT-PCR 实验揭示了香烟烟雾暴露与未处理大鼠的鼻呼吸道上皮细胞中 RELMgamma mRNA 的抑制。 生理组织特异性表达的分析揭示了造血组织中的最高表达，表明 RELM-γ 具有细胞因子样作用。 在骨髓、脾脏和肺以及外周血粒细胞中可检测到 RELM-γ-mRNA。 与模拟转染的细胞相比，用 RELM-γ 表达质粒转染的早幼粒细胞 HL60 细胞具有增加的增殖率，并显示出对视黄酸诱导的粒细胞分化的反应改变。 总之，这些数据提供了第一个实验证据，证明 RELM-γ 是一种具有受限表达模式的分泌分子，可能在早幼粒细胞分化中起作用。

Description

RELM-gamma Mouse Recombinant is a His -Tagged Fusion Protein having a molecular weight of 11 kDa containing 86 amino acid residues of the RELM-gamma Mouse and 16 additional amino acid residues – HisTag .
RELM-gamma Mouse Recombinant 是一种带 His 标签的融合蛋白，分子量为 11 kDa，含有 RELM-gamma Mouse 的 86 个氨基酸残基和 16 个额外的氨基酸残基——HisTag。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Solubility

It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入 0.1M 醋酸盐缓冲液 pH4 制备约 0.5 mg/ml 的工作原液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10µg/ml 的浓度。 在较高浓度下，这种抗原的溶解度是有限的。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。
在 -20°C 下储存时，冻干蛋白在有效期内保持稳定。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

MRGSHHHHHH GMASHMTLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • RELM g Mouse

ProSpec-Resistin Human/CYT-456

ProSpec-Resistin Human,ProSpec CYT-456,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割

Resistin Human
Resistin Human Recombinant
CYT-456
5µg
25µg
1mg

Catalogue number

CYT-456

Synonyms

Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Introduction

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF and FIZZ3 . Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity. Steppan et al. have suggested that resistin suppresses the ability to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。抵抗素以二聚体蛋白的形式在人体血液中循环，该二聚体蛋白由两个 92 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们的敏感性。 Steppan 等人。已经表明抵抗素抑制刺激葡萄糖摄取的能力。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且通过禁食和抗糖尿病药物下调。另一方面，Way 等人发现，抵抗素的表达在肥胖症中受到严重抑制，并受到几种抗糖尿病药物的刺激。
其他研究表明，小鼠抵抗素在脂肪细胞分化期间增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

Description

Resistin Human Recombinant produced in e.coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 93 amino acids and having a total molecular weight of 19.7kDa.
The Resistin Human Recombinant protein is purified by standard chromatographic techniques.

在大肠杆菌中产生的 Resistin Human Recombinant 是一种同型二聚体、非糖基化多肽链，含有 2 x 93 个氨基酸，总分子量为 19.7kDa。
Resistin Human Recombinant 蛋白通过标准色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Purity

Greater than 95% as determined by -PAGE.

Formulation

Sterile filtered and lyophilized from 0.1% Trifluoroacetic Acis .

Solubility

It is recommended to reconstitute the lyophilized Resistin in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在无菌 18MΩ-cm H2O 中以 100µg/ml 的浓度重构冻干的 Resistin，然后可以将其进一步稀释成其他水溶液。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MSSKTLCSME EAINERIQEV AGSLIFRAIS SIGLECQSVT SRGDLATCPR GFAVTGCTCG SACGSWDVRA ETTCHCQCAG MDWTGARCCR VQP

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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  • Resistin Human, HEK

ProSpec-Resistin Human, HEK/CYT-1183

ProSpec-Resistin Human HEK,ProSpec CYT-1183,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Resistin Human, HEK
Resistin Human Recombinant, HEK
CYT-1183
2µg
10µg
1mg

Catalogue number

CYT-1183

Synonyms

RETN,ADSF,FIZZ3,RETN1,RSTN,XCP1,resistin precursor, Adipose tissue-specific secretory factor, ADSFMGC126609, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3; FIZZ3MGC126603, found in inflammatory zone 3, HXCP1.

Introduction

Resistin, also known as adipose tissue-specific secretory factor is a cysteine-rich peptide derived from adipose tissue. Resistin takes part in the inflammatory response, glucose metabolism, and angiogenesis.Resistin blocks insulinstimulated uptake of glucose by adipocytes and promote glucose release by hepatocytes. As such,Resistin considered to participate in diet‑induced insulin-sensitivity. Resistin causes high levels of lowdensity lipoprotein , increasing the risk of heart disease.

抵抗素，也称为脂肪组织特异性分泌因子，是一种来源于脂肪组织的富含半胱氨酸的肽。 抵抗素参与炎症反应、葡萄糖代谢和血管生成。抵抗素阻断胰岛素刺激的脂肪细胞摄取葡萄糖并促进肝细胞释放葡萄糖。 因此，抵抗素被认为参与了饮食诱导的胰岛素敏感性。 抵抗素会导致高水平的低密度脂蛋白，增加患心脏病的风险。

Description

Resistin Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain containing 96 amino acids and having a molecular mass of 10.3 kDa.
Resistin is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

在 HEK293 细胞中产生的 Resistin Human Recombinant 是单条糖基化多肽链，含有 96 个氨基酸，分子量为 10.3 kDa。
抵抗素在 C 末端与 6 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

HEK293 cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

Resistin protein contains 20% glycerol and 20mM Sodium citrate .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE. 

Amino acid sequence

KTLCSMEEAI NERIQEVAGS LIFRAISSIG LECQSVTSRG DLATCPRGFA VTGCTCGSAC GSWDVRAETT CHCQCAGMDW TGARCCRVQP HHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Resistin Human, His/CYT-256

ProSpec-Resistin Human His,ProSpec CYT-256,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Greater than 95% as determined by -PAGE.

Resistin Human, His
Resistin Human Recombinant, His Tag
CYT-256
5µg
25µg
1mg

Catalogue number

CYT-256

Synonyms

Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Introduction

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF and FIZZ3 . Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。抵抗素以二聚体蛋白的形式在人体血液中循环，该二聚体蛋白由两个 92 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
抵抗素可能是肥胖和胰岛素抵抗之间的重要联系。由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们对胰岛素的敏感性。 Steppan 等人。已经提出抵抗素抑制胰岛素刺激葡萄糖摄取的能力。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且被禁食和抗糖尿病药物下调。另一方面，Way 等人发现抵抗素的表达在肥胖症中受到严重抑制，并受到几种抗糖尿病药物的刺激。
其他研究表明，小鼠抵抗素在脂肪细胞分化过程中增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

Description

Resistin Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing a 92 amino acids fragment of the mature Human Resistin, having a total molecular mass of 14.23kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The Resistin is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Resistin Human Recombinant 是一种单一的、非糖基化的多肽链，含有成熟的 Human Resistin 的 92 个氨基酸片段，总分子量为 14.23kDa，并与 4.5kDa 的氨基末端六组氨酸标签融合。
Resistin 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

Resistin protein solution is supplied in 20mM Tris-HCl pH 8.0, 5mM EDTA and 50% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
请避免冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Usage

Prospec’s products are furnished forLABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • Resistin Rat, His
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ProSpec-Resistin Mouse/CYT-1034

ProSpec-Resistin Mouse,ProSpec CYT-1034,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。

Resistin Mouse

Resistin Mouse Recombinant
CYT-1034
ProSpec-Resistin Mouse/CYT-1034 5µg
ProSpec-Resistin Mouse/CYT-1034 25µg
ProSpec-Resistin Mouse/CYT-1034 1mg

Catalogue number

CYT-1034

Synonyms

Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Introduction

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF and FIZZ3 . Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。抵抗素以二聚体蛋白的形式在人体血液中循环，该二聚体蛋白由两个 92 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
抵抗素可能是肥胖和胰岛素抵抗之间的重要联系。由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们对胰岛素的敏感性。 Steppan 等人。已经提出抵抗素抑制胰岛素刺激葡萄糖摄取的能力。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且被禁食和抗糖尿病药物下调。另一方面，Way 等人发现抵抗素的表达在肥胖症中受到严重抑制，并受到几种抗糖尿病药物的刺激。
其他研究表明，小鼠抵抗素在脂肪细胞分化过程中增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

Description

Resistin Mouse Recombinant produced in E.Coli is a non glycosylated, homodimeric polypeptide chain containing 2 x 95 amino acids and having a total molecular mass of 20.6kDa.

The Resistin is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Resistin Mouse Recombinant 是一种非糖基化的同型二聚体多肽链，含有 2 x 95 个氨基酸，总分子量为 20.6kDa。

Resistin 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid .

Solubility

It is recommended to reconstitute the lyophilized Resistin in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在无菌 18MΩ-cm H2O 中以 100µg/ml 的浓度重构冻干的 Resistin，然后可以将其进一步稀释成其他水溶液。

Stability

Lyophilized Resistin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Resistin Mouse should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein .

冻干的抵抗素虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组 Resistin 后，小鼠应在 4°C 下保存 2-7 天，以备将来在 -18°C 以下使用。

对于长期储存，建议添加载体蛋白。

Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MSSMPLCPID EAIDKKIKQD FNSLFPNAIK NIGLNCWTVS SRGKLASCPE GTAVLSCSCG SACGSWDIRE EKVCHCQCAR IDWTAARCCK LQVAS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Resistin Mouse, Flag/CYT-457

ProSpec-Resistin Mouse, Flag,ProSpec CYT-457,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。

Resistin Mouse, Flag

Resistin Mouse Recombinant, Flag Tag
CYT-457
ProSpec-Resistin Mouse, Flag/CYT-457 5µg
ProSpec-Resistin Mouse, Flag/CYT-457 25µg
ProSpec-Resistin Mouse, Flag/CYT-457 1mg

Catalogue number

CYT-457

Synonyms

Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Introduction

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF and FIZZ3 . Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。抵抗素以二聚体蛋白的形式在人体血液中循环，该二聚体蛋白由两个 92 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
抵抗素可能是肥胖抵抗之间的重要联系。由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们的敏感性。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且被禁食和抗糖尿病药物下调。另一方面，Way 等人发现抵抗素的表达在肥胖症中受到严重抑制，并受到几种抗糖尿病药物的刺激。
其他研究表明，小鼠抵抗素在脂肪细胞分化过程中增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

Description

Resistin Mouse is manufactured with signal sequence of phage fd and C-terminal fusion of flagTag . Resistin Mouse Recombinant Flag-Tagged Fusion Protein is 13.7 kDa protein containing 93 amino acid residues of the Resistin Mouse and 31 additional amino acid residues – signal sequence of phage fd, flagTag .
Resistin Mouse 是用 phage fd 的信号序列和 flagTag 的 C 端融合制造的。 Resistin Mouse Recombinant Flag-Tagged Fusion Protein 是一种 13.7 kDa 的蛋白质，含有 Resistin Mouse 的 93 个氨基酸残基和 31 个额外的氨基酸残基 – 噬菌体 fd，flagTag 的信号序列。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Solubility

It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入 0.1M 醋酸盐缓冲液 pH4 配制约 0.5 mg/mL 的工作原液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10μg/ml 的浓度。 在较高浓度下，该抗原的溶解度是有限的。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Amino acid sequence

MKKLLFAIPL VVPFYSHSTM ASMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVASLEDYKD DDDK.

Purity

Greater than 95% as determined by -PAGE.

Usage

Prospec’s products are furnished forLABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • Resistin Human
  • Resistin Mutant Human
  • Resistin Rat, His
  • Resistin Human, His
  • Resistin Mouse
  • Resistin Human, HEK

ProSpec-Resistin Mutant Human/CYT-585

ProSpec-Resistin Mutant Human,ProSpec CYT-585,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,抵抗素、富含半胱氨酸的分泌蛋白 FIZZ3、脂肪组织特异性分泌因子、ADSF、C/EBP-ε-调节骨髓特异性分泌的富含半胱氨酸蛋白、富含半胱氨酸的分泌蛋白 A12-α 样 2、RSTN、XCP1、 RETN1、MGC126603、MGC126609。

Resistin Mutant Human
Resistin Mutant Human Recombinant
CYT-585
ProSpec-Resistin Mutant Human/CYT-585 2µg
ProSpec-Resistin Mutant Human/CYT-585 10µg
ProSpec-Resistin Mutant Human/CYT-585 1mg

Catalogue number

CYT-585

Synonyms

Resistin, Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.
抵抗素、富含半胱氨酸的分泌蛋白 FIZZ3、脂肪组织特异性分泌因子、ADSF、C/EBP-ε-调节骨髓特异性分泌的富含半胱氨酸蛋白、富含半胱氨酸的分泌蛋白 A12-α 样 2、RSTN、XCP1、 RETN1、MGC126603、MGC126609。

Introduction

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF and FIZZ3 . Human resistin contains 108 amino acids as a prepeptide, and its hydrophobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
抵抗素是RSTN基因的产物，是一种肽激素，属于富含半胱氨酸的分泌蛋白类，被称为RELM家族，也被描述为ADSF和FIZZ3。人抵抗素含有108个氨基酸的前肽，其疏水信号肽在分泌前被切割。抵抗素以二聚体蛋白的形式在人体血液中循环，该二聚体蛋白由两个 92 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
抵抗素可能是肥胖和胰岛素抵抗之间的重要联系。由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们对胰岛素的敏感性。 Steppan 等人。已经提出抵抗素抑制胰岛素刺激葡萄糖摄取的能力。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且通过禁食和抗糖尿病药物下调。另一方面，Way 等人发现抵抗素的表达在肥胖症中受到严重抑制，并且受到几种抗糖尿病药物的刺激。
其他研究表明，小鼠抵抗素在脂肪细胞分化期间增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

Description

9.9 kDa protein containing 93 amino acid residues, produced in E.coli. Mutant-Resistin has had a Cysteine residue mutated to prevent dimerization and possibly acts as an antagonist.
含有 93 个氨基酸残基的 9.9 kDa 蛋白质，在大肠杆菌中产生。 Mutant-Resistin 有一个半胱氨酸残基突变以防止二聚化并可能充当拮抗剂。

Source

Escherichia Coli.

Formulation

Recombinant Human Resistin was lyophilized from a concentrated solution containing 0.1% TFA.

Solubility

Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Amino acid sequence

MSSKTLCSME EAINERIQEV AGSLIFRAIS SIGLECQSVT SRGDLATCPR GFAVTGCTCG SACGSWDVRA ETTCHCQCAG MDWTGARCCR VQP.

Purity

Greater than 95% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Resistin Rat, His/CYT-458

ProSpec-Resistin Rat His,ProSpec CYT-458,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,抵抗素是 RSTN 基因的产物，是一种肽激素，属于称为 RELM 家族的富含半胱氨酸的分泌蛋白类，也被描述为 ADSF 或 FIZZ3。小鼠抵抗素表达为 114 个氨基酸的前肽；它的疏水性 N 末端 20 个氨基酸信号肽在其分泌之前被切割。

Resistin Rat, His
Resistin Rat Recombinant, His Tag
CYT-458
ProSpec-Resistin Rat, His/CYT-458 5µg
ProSpec-Resistin Rat, His/CYT-458 25µg
ProSpec-Resistin Rat, His/CYT-458 1mg

Catalogue number

CYT-458

Synonyms

Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Introduction

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins referred to as the RELM family, and is also described as ADSF or FIZZ3 . Mouse resistin is expressed as a 114 amino acid prepeptide; its hydrofobic Nterminal 20 amino acid signal peptide is cleaved before its secretion. Mouse resistin circulates in blood as a homodimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity. Steppan et al. have suggested that resistin suppressed the ability to stimulate glucose uptake. They have also suggested that resistin was present at elevated levels in blood of obese mice, and was down regulated by fasting and by antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in obesity.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

抵抗素是 RSTN 基因的产物，是一种肽激素，属于称为 RELM 家族的富含半胱氨酸的分泌蛋白类，也被描述为 ADSF 或 FIZZ3。小鼠抵抗素表达为 114 个氨基酸的前肽；它的疏水性 N 末端 20 个氨基酸信号肽在其分泌之前被切割。小鼠抵抗素作为同源二聚体蛋白在血液中循环，该蛋白由两个 94 个氨基酸的多肽组成，它们通过 Cys26 二硫键连接。
抵抗素可能是肥胖之间的重要联系。由脂肪细胞特异性产生和分泌的小鼠抵抗素作用于骨骼肌肌细胞、肝细胞和脂肪细胞本身，从而降低它们的敏感性。 Steppan 等人。已经表明抵抗素抑制了刺激葡萄糖摄取的能力。他们还提出，肥胖小鼠血液中的抵抗素水平升高，并且通过禁食和抗糖尿病药物下调。另一方面，Way 等人发现抵抗素的表达在肥胖症中受到严重抑制。
其他研究表明，小鼠抵抗素在脂肪细胞分化期间增加，但它似乎也抑制脂肪生成。相反，人类脂肪形成分化可能与抵抗素基因表达的下调有关。

Description

Resistin Rat Recombinant is manufactured with N-terminal fusion of His tag.
Resistin Rat Recombinant His-Tagged Fusion Protein is an 11.9 kDa protein containing 94 amino acid residues of the Resistin Rat and 16 additional amino acid residues – His Tag .
Resistin Rat Recombinant 是用 His 标签的 N 端融合制造的。
Resistin Rat Recombinant His-Tagged Fusion Protein 是一种 11.9 kDa 的蛋白质，含有 Resistin Rat 的 94 个氨基酸残基和 16 个额外的氨基酸残基 – His 标签。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5mg/ml in 20mM Tris pH 8.0.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

MRGSHHHHHH GMASHMPSMS LCPMDEAISK KINQDFSSLL PAAMKNTVLH CWSVSSRGRL ASCPEGTTVT SCSCGSGCGS WDVREDTMCH CQCGSIDWTA ARCCTLRVGS.

Usage

Prospec’s products are furnished forLABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-SAA Human/CYT-942

ProSpec-SAA Human,ProSpec CYT-942,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Human SAA was lyophilized from a concentrated solution in 20mM Tris-HCl, pH 9.0 and 150mM NaCl.

SAA Human
Serum Amyloid A Human Recombinant
CYT-942
ProSpec-SAA Human/CYT-942 10µg
ProSpec-SAA Human/CYT-942 50µg
ProSpec-SAA Human/CYT-942 1mg

Catalogue number

CYT-942

Introduction

Serum amyloid A protein which is a member of the SAA family is encoded by the SAA gene in humans. SAA is expressed by the liver and secreted in plasma. Serum amyloid A is the main acute phase reactant and apolipoprotein of the HDL complex. Elevated serum SAA protein levels may be linked with lung cancer. The level of Apo-SAA, which is ordinarily 1-5 ?g/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL Apolipoprotein.
作为SAA家族成员的血清淀粉样蛋白A由人类的SAA基因编码。 SAA由肝脏表达并分泌到血浆中。 血清淀粉样蛋白 A 是 HDL 复合物的主要急性期反应物和载脂蛋白。 血清SAA蛋白水平升高可能与肺癌有关。 血浆中的 Apo-SAA 水平通常为 1-5 μg/ml，在炎症刺激后 24 小时内增加 500-1000 倍，在这些条件下，它是最丰富的 HDL 载脂蛋白。

Description

APO-SAA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 104 amino acids and having a molecular mass of 11.7kDa. Recombinant Apo-SAA is a consensus SAA molecule corresponding to human Apo-SAA1a, except for the presence of an N-terminal methionine, the substitution of asparagine for aspartic acid at position 60, and arginine for histidine at position 71 .
The Human APO-SAA is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 APO-SAA Human Recombinant 是一条单一的、非糖基化的多肽链，包含 104 个氨基酸，分子量为 11.7kDa。 重组 Apo-SAA 是一种与人 Apo-SAA1a 相对应的共有 SAA 分子，除了存在一个 N 端甲硫氨酸、60 位天冬氨酸取代天冬氨酸和 71 位精氨酸取代组氨酸。
人 APO-SAA 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Human SAA was lyophilized from a concentrated solution in 20mM Tris-HCl, pH 9.0 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized SAA Human in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中重新配制冻干的 SAA Human，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Human SAA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human SAA should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
冻干的人 SAA 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，人 SAA 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISN ARENIQRFFG RGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY.

Biological Activity

The biological activity determined by its ability to down-regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4µM.
由其下调主动脉平滑肌细胞中脂质生物合成的能力决定的生物活性。 发现有效浓度为4μM。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec-SAA1 Human/CYT-787

ProSpec-SAA1 Human,ProSpec CYT-787,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,通过使用人单核细胞的化学吸引生物测定法确定的生物活性在 10-100 ng/ml 的浓度范围内。

SAA1 Human

Serum Amyloid A Human Recombinant
CYT-787
ProSpec-SAA1 Human/CYT-787 10µg
ProSpec-SAA1 Human/CYT-787 50µg
ProSpec-SAA1 Human/CYT-787 1mg

Catalogue number

CYT-787

Synonyms

Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.

Introduction

SAA1 protein is an acute phase apolipoprotein reactant which is produced mostly by hepatocytes and under regulation of inflammatory cytokines. SAA1 protein is produced mainly in the liver and circulates in low levels in the blood. The SAA1 seems to have a role in the immune system. SAA1 protein levels increase in the blood and other tissues under conditions of inflammation. SAA1 may facilitate the repair of injured tissues; it also acts as an antibacterial agent, and signals the migration of germ-fighting cells to sites of infection. SAA1 also functions as an apolipoprotein of the HDL complex.
Elevated levels of SAA1 ultimately affect secondary amyloidosis, extracellular amassing of amyloid fibrils, resulting from a circulating precursor, in a variety of tissues and organs. The most widespread type of amyloidosis appears secondary to chronic inflammatory disease, mainly rheumatoid arthritis. The SAA1 cleavage product a designated amyloid protein A is deposited systemically as amyloid in vital organs such as the liver, spleen, and kidneys in chronic inflammatory diseases patients. These deposits are extremely insoluble and resistant to proteolysis; they disrupt tissue structure and compromise performance.
SAA1蛋白是一种急性期载脂蛋白反应物，主要由肝细胞产生，在炎性细胞因子的调节下。 SAA1 蛋白主要在肝脏中产生，并在血液中以低水平循环。 SAA1 似乎在免疫系统中起作用。在炎症条件下，血液和其他组织中的 SAA1 蛋白水平会升高。 SAA1 可促进受损组织的修复；它还可以作为一种抗菌剂，并发出抗细菌细胞迁移到感染部位的信号。 SAA1 还作为 HDL 复合物的载脂蛋白发挥作用。
SAA1 水平升高最终会影响多种组织和器官中的继发性淀粉样变性、淀粉样蛋白原纤维的细胞外聚集，这是由循环前体引起的。最普遍的淀粉样变性类型似乎继发于慢性炎症性疾病，主要是类风湿性关节炎。 SAA1 裂解产物称为淀粉样蛋白 A 以淀粉样蛋白的形式全身沉积在慢性炎性疾病患者的肝脏、脾脏和肾脏等重要器官中。这些沉积物极难溶解，对蛋白水解有抵抗力；它们会破坏组织结构并损害性能。

Description

SAA1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 104 amino acids and having a molecular mass of 11.7kDa.
The SAA1 is purified by proprietary chromatographic teichnques.
在大肠杆菌中产生的 SAA1 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 104 个氨基酸，分子量为 11.7kDa。
SAA1 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

SAA1 was lyophilized from a 0.2µm filtered concentrated solution in 20mM Tris-HCl, pH 9.0 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized SAA1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 SAA1，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized SAA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SAA1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SAA1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，SAA1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY.

Biological Activity

The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
通过使用人单核细胞的化学吸引生物测定法确定的生物活性在 10-100 ng/ml 的浓度范围内。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-SAA1 Human, His/CYT-675

ProSpec-SAA1 Human His,ProSpec CYT-675,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,SAA1蛋白是一种急性期载脂蛋白反应物，主要由肝细胞产生，在炎性细胞因子的调节下。 SAA1 蛋白主要在肝脏中产生，并在血液中以低水平循环。

SAA1 Human, His

Serum Amyloid A Human Recombinant , His Tag
CYT-675
ProSpec-SAA1 Human, His/CYT-675 10µg
ProSpec-SAA1 Human, His/CYT-675 50µg
ProSpec-SAA1 Human, His/CYT-675 1mg

Catalogue number

CYT-675

Synonyms

Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.

Introduction

SAA1 protein is an acute phase apolipoprotein reactant which is produced mostly by hepatocytes and under regulation of inflammatory cytokines. SAA1 protein is produced mainly in the liver and circulates in low levels in the blood. The SAA1 seems to have a role in the immune system. SAA1 protein levels increase in the blood and other tissues under conditions of inflammation. SAA1 may facilitate the repair of injured tissues; it also acts as an antibacterial agent, and signals the migration of germ-fighting cells to sites of infection. SAA1 also functions as an apolipoprotein of the HDL complex.
Elevated levels of SAA1 ultimately affect secondary amyloidosis, extracellular amassing of amyloid fibrils, resulting from a circulating precursor, in a variety of tissues and organs. The most widespread type of amyloidosis appears secondary to chronic inflammatory disease, mainly rheumatoid arthritis. The SAA1 cleavage product a designated amyloid protein A is deposited systemically as amyloid in vital organs such as the liver, spleen, and kidneys in chronic inflammatory diseases patients. These deposits are extremely insoluble and resistant to proteolysis; they disrupt tissue structure and compromise performance.
SAA1蛋白是一种急性期载脂蛋白反应物，主要由肝细胞产生，在炎性细胞因子的调节下。 SAA1 蛋白主要在肝脏中产生，并在血液中以低水平循环。 SAA1 似乎在免疫系统中起作用。在炎症条件下，血液和其他组织中的 SAA1 蛋白水平会升高。 SAA1 可促进受损组织的修复；它还可以作为一种抗菌剂，并发出抗细菌细胞迁移到感染部位的信号。 SAA1 还作为 HDL 复合物的载脂蛋白发挥作用。
SAA1 水平升高最终会影响多种组织和器官中的继发性淀粉样变性、淀粉样蛋白原纤维的细胞外聚集，这是由循环前体引起的。最普遍的淀粉样变性类型似乎继发于慢性炎症性疾病，主要是类风湿性关节炎。 SAA1 裂解产物称为淀粉样蛋白 A 以淀粉样蛋白的形式全身沉积在慢性炎性疾病患者的肝脏、脾脏和肾脏等重要器官中。这些沉积物极难溶解，对蛋白水解有抵抗力；它们会破坏组织结构并损害性能。

Description

SAA1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a total molecular mass of 13.9 kDa.
SAA1 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 SAA1 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 125 个氨基酸，总分子量为 13.9 kDa。
SAA1 在 N 末端与 20 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The SAA1 solution contains 20mM Tris buffer and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS DARENIQRFF GHGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • SAA1 Monkey
  • SAA1 Human

ProSpec-SAA4 Human/CYT-002,Serum Amyloid A4 Human Recombinant

ProSpec-SAA4 Human/CYT-002,Serum Amyloid A4 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,SAA4 Human Recombinant 在 N 末端融合了 21 个氨基酸的 His 标签，在大肠杆菌中产生，是一条含有 131 个氨基酸且分子量为 14.9kDa 的单一非糖基化多肽链。 SAA4 通过专有的色谱技术进行纯化。

SAA4 Human
Serum Amyloid A4 Human Recombinant
CYT-002
2µg
10µg
1mg

Catalogue number

CYT-002

Synonyms

Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein, C-SAA, SAA4, CSAA.

Introduction

SAA4 is a constitutively expressed protein which is a member of the SAA family. SAA4 is a major acute phase reactant and an apolipoprotein of the HDL complex. SAA4 is constitutively expressed only in humans and mice; it is connected almost completely with lipoproteins of the high density range. The physiological function of SAA4 is unknown, and its serum concentration has no association with those of other major apolipoproteins. SAA4 mRNA and protein occurrence in macrophage derived foam cells of coronary and carotid arteries implied a specific role of human SAA4 during inflammation including atherosclerosis.
SAA4是组成型表达的蛋白质，是SAA家族的成员。 SAA4 是主要的急性期反应物和 HDL 复合物的载脂蛋白。 SAA4 仅在人和小鼠中组成型表达； 它几乎完全与高密度范围的脂蛋白相连。 SAA4 的生理功能未知，其血清浓度与其他主要载脂蛋白的浓度无关。 SAA4 mRNA 和蛋白质在冠状动脉和颈动脉巨噬细胞衍生的泡沫细胞中的出现暗示了人类 SAA4 在炎症（包括动脉粥样硬化）过程中的特定作用。

Description

SAA4 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 131 amino acids and having a molecular mass of 14.9kDa. The SAA4 is purified by proprietary chromatographic techniques.
SAA4 Human Recombinant 在 N 末端融合了 21 个氨基酸的 His 标签，在大肠杆菌中产生，是一条含有 131 个氨基酸且分子量为 14.9kDa 的单一非糖基化多肽链。 SAA4 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The SAA4 solution contains 20mM Tris-HCl buffer , 20% glycerol, 5mM DTT, 0.2M NaCl and 0.5mM EDTA.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MWRSFFKEAL QGVGDMGRAY WDIMISNHQN SNRYLYARGN YDAAQRGPGG VWAAKLISRS RVYLQGLIDY YLFGNSSTVL EDSKSNEKAE EWGRSGKDPD RFRPDGLPKK Y.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-Thrombopoietin Human/CYT-1178,Thrombopoietin Human Recombinant

ProSpec-Thrombopoietin Human/CYT-1178,Thrombopoietin Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,血小板生成素是一种糖蛋白激素，主要由肝脏和肾脏产生，可调节骨髓的血小板生成。 TPO 刺激巨核细胞的产生和分化，巨核细胞是分裂成大量血小板的骨髓细胞。

Thrombopoietin Human

Thrombopoietin Human Recombinant
CYT-1178
2µg
10µg
1mg

Catalogue number

CYT-1178

Synonyms

Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO

Introduction

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets.

血小板生成素是一种糖蛋白激素，主要由肝脏和肾脏产生，可调节骨髓的血小板生成。 TPO 刺激巨核细胞的产生和分化，巨核细胞是分裂成大量血小板的骨髓细胞。

Description

TPO Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain containing 343 amino acids and having a molecular mass of 36.8kDa.
TPO  is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

在 HEK293 细胞中产生的 TPO Human Recombinant 是单条糖基化多肽链，含有 343 个氨基酸，分子量为 36.8kDa。
TPO 在 C 端与 6 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

HEK293 cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TPO protein contains 10% glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

The ED50 range is ≤10ng/ml. It is measured by cell proliferation assay using MO7e human megakaryocytic leukemic cells.

Amino acid sequence

DGSHMSPAPP ACDLRVLSKL LRDSHVLHSR LSQCPEVHPL PTPVLLPAVD FSLGEWKTQM EETKAQDILG AVTLLLEGVM AARGQLGPTC LSSLLGQLSG QVRLLLGALQ SLLGTQLPPQ GRTTAHKDPN AIFLSFQHLL RGKVRFLMLV GGSTLCVRRA PPTTAVPSRT SLVLTLNELP NRTSGLLETN FTASARTTGS GLLKWQQGFR AKIPGLLNQT SRSLDQIPGY LNRIHELLNG TRGLFPGPSR RTLGAPDISS GTSDTGSLPP NLQPGYSPSP THPPTGQYTL FPLPPTLPTP VVQLHPLLPD PSAPTPTPTS PLLNTSYTHS QNLSQEGHHH HHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TPO Human, HEK
  • TPO Human, His
  • TPO Mouse
  • TPO Mouse, HEK
  • TPO Human
  • TPO Human, Biotin
  • TPO Human, CHO

ProSpec-TPO Human, CHO/CYT-1070,Thrombopoietin Human Recombinant, CHO

ProSpec-TPO Human, CHO/CYT-1070,Thrombopoietin Human Recombinant, CHO,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,通过 MO7e 细胞的剂量依赖性刺激确定的 ED50 对应于 3×10 5 单位/mg 的比活性。

TPO Human, CHO
Thrombopoietin Human Recombinant, CHO
CYT-1070
1µg
5µg
50µg

Catalogue number

CYT-1070

Synonyms

MPL Ligand, THCYT1, MPLLG, TPO, ML, Thrombopoietin Nirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth and Development Factor, Megakaryocyte Colony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte Stimulating Factor, Prepro-Thrombopoietin.

Introduction

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets.

血小板生成素是一种糖蛋白激素，主要由肝脏和肾脏产生，可调节骨髓的血小板生成。 TPO 刺激巨核细胞的产生和分化，巨核细胞是分裂成大量血小板的骨髓细胞。

Description

Thrombopoietin Human Recombinant produced in CHO cells has a molecular weight range of 80-90kDa due to glycosylation.
The TPO is purified by proprietary chromatographic techniques.

由于糖基化，在 CHO 细胞中产生的 Thrombopoietin Human Recombinant 具有 80-90kDa 的分子量范围。
TPO 通过专有的色谱技术进行纯化。

Source

Chinese Hamster Ovary Cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TPO protein solution contains phosphate buffered saline and 2% albumin.

Stability

Thrombopoietin although stable at room temperature for 1 week, should be stored between 2-8°C.

Purity

Greater than 98% as determined by -PAGE.

Biological Activity

The ED50 as determined by the dose-dependent stimulation of MO7e cells corresponding to a specific activity of 3×105 units/mg.

通过 MO7e 细胞的剂量依赖性刺激确定的 ED50 对应于 3×10 5 单位/mg 的比活性。

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TPO Human, HEK
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  • TPO Human, His
  • TPO Human
  • TPO Mouse, HEK
  • Thrombopoietin Human

ProSpec-TPO Human, HEK/CYT-115,Thrombopoietin Human Recombinant, HEK

ProSpec-TPO Human, HEK/CYT-115,Thrombopoietin Human Recombinant, HEK,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,冻干的血小板生成素虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TPO 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

TPO Human, HEK
Thrombopoietin Human Recombinant, HEK
CYT-115
2µg
10µg
1mg

Catalogue number

CYT-115

Synonyms

Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.
巨核细胞集落刺激因子、骨髓增殖性白血病病毒癌基因配体、C-mpl配体、ML、巨核细胞生长发育因子、MGDF、TPO、MKCSF、MPLLG、MGC163194、THPO。

Introduction

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets.
血小板生成素是一种糖蛋白激素，主要由肝脏和肾脏产生，可调节骨髓的血小板生成。 它刺激巨核细胞的产生和分化，巨核细胞是分裂成大量血小板的骨髓细胞。

Description

Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.
The TPO is purified by proprietary chromatographic techniques.
HEK 细胞中产生的血小板生成素人重组体是一种糖基化单体，由于糖基化，其分子量范围为 80-85kDa。
TPO 通过专有的色谱技术进行纯化。

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The TPO protein was filtered and lyophilized from 0.74mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized Thrombopoietin in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Stability

Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的血小板生成素虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TPO 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95% as obsereved by -PAGE.

Biological Activity

The activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells, the ED50 is 1.15ng/ml.

Amino acid sequence

SPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLG EWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQV RLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGG STLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLK WQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAP DISSGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPTPVVQLHPLLPDPSAPTPT

PTSPLLNTSYTHSQNLSQEG

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-TPO Human, His/CYT-890,Thrombopoietin Human Recombinant, His Tag

ProSpec-TPO Human, His/CYT-890,Thrombopoietin Human Recombinant, His Tag,MPL 配体、THCYT1、MPLLG、TPO、ML、血小板生成素 Nirs 变体 1、MKCSF、血小板生成素、骨髓增殖性白血病病毒癌基因配体、巨核细胞生长和发育因子、巨核细胞集落刺激因子、C-Mpl 配体、MGDF、巨核细胞刺激因子、前体 – 血小板生成素。

TPO Human, His
Thrombopoietin Human Recombinant, His Tag
CYT-890
2µg
10µg
1mg

Catalogue number

CYT-890

Synonyms

MPL Ligand, THCYT1, MPLLG, TPO, ML, Thrombopoietin Nirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth and Development Factor, Megakaryocyte Colony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte Stimulating Factor, Prepro-Thrombopoietin.
MPL 配体、THCYT1、MPLLG、TPO、ML、血小板生成素 Nirs 变体 1、MKCSF、血小板生成素、骨髓增殖性白血病病毒癌基因配体、巨核细胞生长和发育因子、巨核细胞集落刺激因子、C-Mpl 配体、MGDF、巨核细胞刺激因子、前体 – 血小板生成素。

Introduction

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets.

Description

TPO produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 340 amino acids and having a molecular mass of 36.5kDa. .
TPO is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques..
在 Sf9 杆状病毒细胞中产生的 TPO 是单条糖基化多肽链，含有 340 个氨基酸，分子量为 36.5kDa。 .
TPO 在 C 末端用 8 个氨基酸 His 标签表达，并通过专有色谱技术纯化。

Source

Sf9, Insect cell.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TPO protein solution contains phosphate buffered saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG
LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGLEHHHHHH.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

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ProSpec-TPO Mouse/CYT-346,Thrombopoietin Mouse Recombinant

ProSpec-TPO Mouse/CYT-346,Thrombopoietin Mouse Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,血小板生成素是一种糖蛋白激素，主要由肝脏产生，肾帽调节骨髓血小板的产生。 它刺激巨核细胞的产生和分化，巨核细胞是分裂成大量血小板的骨髓细胞。

TPO Mouse
Thrombopoietin Mouse Recombinant
CYT-346
2µg
10µg
1mg

Catalogue number

CYT-346

Synonyms

Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.
巨核细胞集落刺激因子、骨髓增殖性白血病病毒癌基因配体、C-mpl配体、ML、巨核细胞生长发育因子、MGDF、TPO、MKCSF、MPLLG、MGC163194、THPO。

Introduction

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidneyt hat regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets.
血小板生成素是一种糖蛋白激素，主要由肝脏产生，肾帽调节骨髓血小板的产生。 它刺激巨核细胞的产生和分化，巨核细胞是分裂成大量血小板的骨髓细胞。

Description

Thrombopoietin Mouse Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a molecular mass of 18704 Dalton. The TPO is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的血小板生成素小鼠重组体是一种单一的、非糖基化的可溶性多肽链，含有 174 个氨基酸，分子量为 18704 道尔顿。 TPO 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution in water containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Thrombopoietin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的血小板生成素，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO Mouse should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的血小板生成素虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TPO 小鼠应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF.

Biological Activity

ED50 range is less than 0.8ng/ml as determined by the dose dependent stimulation of Mo7e cells.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TPO Human, His
  • TPO Human, HEK
  • Thrombopoietin Human

ProSpec-TPO Mouse, HEK/CYT-1157,Thrombopoietin Mouse Recombinant, HEK

ProSpec-TPO Mouse, HEK/CYT-1157,Thrombopoietin Mouse Recombinant, HEK,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,血小板生成素或 TPO 或 MGDF，是一种可以在肝脏和肾脏组织中找到的蛋白质。 TPO 调节血小板的产生。 该蛋白质增强细胞的产生和分化，例如巨核细胞，巨核细胞是分泌大量血小板的骨髓细胞的一部分。

TPO Mouse, HEK
Thrombopoietin Mouse Recombinant, HEK
CYT-1157
2µg
10µg
1mg

Catalogue number

CYT-1157

Synonyms

Thpo, C-mpl ligand,ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1.

Introduction

Thrombopoietin or TPO or MGDF, is a protein that can be found in the liver and kidney tissues. TPO regulates the creation of platelets. The protein enhances the production & differentiation of cells such as megakaryocytes that are part of the bone marrow cells that secretes a wide number of platelets. The cellular development process that ends up in the production of platelet calls . Humoral growth factor is needed for the megakaryocyte proliferation & maturation of megakaryocyte, not apart from thrombopoiesis.

血小板生成素或 TPO 或 MGDF，是一种可以在肝脏和肾脏组织中找到的蛋白质。 TPO 调节血小板的产生。 该蛋白质增强细胞的产生和分化，例如巨核细胞，巨核细胞是分泌大量血小板的骨髓细胞的一部分。 最终产生血小板的细胞发育过程。 巨核细胞的增殖和成熟需要体液生长因子，除了血小板生成。

Description

TPO Mouse Recombinant produced in HEK293 cells is a single, non-glycosylated polypeptide chain containing  341 amino acids and having a molecular mass of  36.4kDa. TPO is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

在 HEK293 细胞中产生的 TPO 小鼠重组体是一条含有 341 个氨基酸且分子量为 36.4kDa 的单一非糖基化多肽链。 TPO 在 C 端与 6 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

HEK293.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TPO protein contains  Phosphate-Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤4ng/ml.

Biological Activity

Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is ≥ 25ug/ml.

Amino acid sequence

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKFPNRTSG LLETNFSVTA RTAGPGLLSR LQGFRVKITP GQLNQTSRSP VQISGYLNRT HGPVNGTHGL FAGTSLQTLE ASDISPGAFN KGSLAFNLQG GLPPSPSLAP DGHTPFPPSP ALPTTHGSPP QLHPLFPDPS TTMPNSTAPH PVTMYPHPRN LSQETHHHHH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TPO Human
  • TPO Human, Biotin
  • TPO Human, CHO
  • TPO Human, HEK
  • Thrombopoietin Human

ProSpec-TFF1 Human/CYT-586,Trefoil Factor-1 Human Recombinant

ProSpec-TFF1 Human/CYT-586,Trefoil Factor-1 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,三叶因子肽是在胃肠道中表达的稳定分泌蛋白，参与肠黏膜的防御和修复。 TFF1 是胃窦和幽门胃粘膜正常分化的必需蛋白，并作为胃特异性肿瘤抑制基因发挥作用。

TFF1 Human

Trefoil Factor-1 Human Recombinant
CYT-586
5µg
20µg
1mg

Catalogue number

CYT-586

Synonyms

TFF-1, TFF1, pS2, BCEI, HPS, HP1.A, pNR-2, D21S21, pS2 protein, Trefoil factor 1, Breast cancer estrogen-inducible protein.

Introduction

The Trefoil Factor peptides are stable secretory proteins expressed in the gastrointestinal tract , and are involved in intestinal mucosal defense and repair. TFF1 is an essential protein for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene. TFF1 is a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. TFF1 protects the mucosa from isults, stabilizes the mucus layer, & affects healing of the epithelium. TFF1 is commonly expressed in tumors. TFF1 is related with the cell membrane of MCF-7 cells. High levels of TFF1 and TFF2 are found in serum from inflammatory bowel disease.
三叶因子肽是在胃肠道中表达的稳定分泌蛋白，参与肠黏膜的防御和修复。 TFF1 是胃窦和幽门胃粘膜正常分化的必需蛋白，并作为胃特异性肿瘤抑制基因发挥作用。 TFF1 是覆盖在胃肠道黏膜上的黏液凝胶的稳定剂，可提供抵抗各种有害物质的物理屏障。 TFF1 保护粘膜免受损伤，稳定粘液层，并影响上皮的愈合。 TFF1 通常在肿瘤中表达。 TFF1与MCF-7细胞的细胞膜有关。 在炎症性肠病的血清中发现了高水平的 TFF1 和 TFF2。

Description

TFF-1 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 60 amino acids which includes a 40 amino acid trefoil motif containing 3 conserved intramolecular disulfide bonds and having a total molecular mass of 13.2 kDa.
TFF-1 Human Recombinant is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TFF-1 Human Recombinant 是一种同源二聚体、非糖基化多肽链，含有 2 x 60 个氨基酸，其中包括一个 40 个氨基酸的三叶基序，该基序含有 3 个保守的分子内二硫键，总分子量为 13.2 kDa。
TFF-1 Human Recombinant 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The Human TFF1 protein was lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized TFF1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized TFF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TFF1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TFF1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

EAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFY
PNTIDVPPEEECEF.

Biological Activity

The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10µg/ml, corresponding to a specific activity of >100 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TFF3 Human
  • TFF3 Human, His
  • TFF2 Human, His
  • TFF2 Human
  • TFF1 Human, His

ProSpec-TFF1 Human, His/CYT-610,Trefoil Factor-1 Human Recombinant, His Tag

ProSpec-TFF1 Human, His/CYT-610,Trefoil Factor-1 Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines三叶因子肽是在胃肠道中表达的稳定分泌蛋白，参与肠黏膜的防御和修复。 TFF1 是胃窦和幽门胃粘膜正常分化的必需蛋白，并作为胃特异性肿瘤抑制基因发挥作用。

TFF1 Human, His
Trefoil Factor-1 Human Recombinant, His Tag
CYT-610
5µg
20µg
1mg

Catalogue number

CYT-610

Synonyms

TFF-1, TFF1, pS2, BCEI, HPS, HP1.A, pNR-2, D21S21, pS2 protein, Trefoil factor 1, Breast cancer estrogen-inducible protein.

Introduction

The Trefoil Factor peptides are stable secretory proteins expressed in the gastrointestinal tract , and are involved in intestinal mucosal defense and repair. TFF1 is an essential protein for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene. TFF1 is a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. TFF1 protects the mucosa from isults, stabilizes the mucus layer, & affects healing of the epithelium. TFF1 is commonly expressed in tumors. TFF1 is related with the cell membrane of MCF-7 cells. High levels of TFF1 and TFF2 are found in serum from inflammatory bowel disease.
三叶因子肽是在胃肠道中表达的稳定分泌蛋白，参与肠黏膜的防御和修复。 TFF1 是胃窦和幽门胃粘膜正常分化的必需蛋白，并作为胃特异性肿瘤抑制基因发挥作用。 TFF1 是覆盖在胃肠道黏膜上的黏液凝胶的稳定剂，可提供抵抗各种有害物质的物理屏障。 TFF1 保护粘膜免受损伤，稳定粘液层，并影响上皮的愈合。 TFF1 通常在肿瘤中表达。 TFF1与MCF-7细胞的细胞膜有关。 在炎症性肠病的血清中发现了高水平的 TFF1 和 TFF2。

Description

TFF-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids which includes a 10 amino acid His Tag and having a total molecular mass of 7.9 kDa.
TFF-1 Human Recombinant is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TFF-1 Human Recombinant 是一条单一的、非糖基化的多肽链，含有 70 个氨基酸，其中包括一个 10 个氨基酸的 His 标签，总分子量为 7.9 kDa。
TFF-1 Human Recombinant 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

The TFF1 His Tag protein was lyophilized from 0.4μm filtered solution at a concentration of 0.5mg/ml containing 20mM Tris pH-7.5 and 20mM NaCl.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Lyophilized TFF1 His Tag although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF1 His Tag should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TFF1 His 标签虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组 TFF1 后，他的标签应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHAS EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • TFF1 Human
  • TFF3 Human
  • TFF2 Human
  • TFF3 Human, His
  • TFF2 Human, His

ProSpec-TFF2 Human/CYT-612,Trefoil Factor-2 Human Recombinant

ProSpec-TFF2 Human/CYT-612,Trefoil Factor-2 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,The TFF2 protein was lyophilized from 0.4μm filtered solution at a concentration of 1mg/mL containing 1x PBS pH-7.4.

TFF2 Human
Trefoil Factor-2 Human Recombinant
CYT-612
5µg
20µg
1mg

Catalogue number

CYT-612

Synonyms

TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.

Introduction

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains.
TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠黏膜中表达的稳定分泌蛋白，可保护黏膜免受损伤、稳定黏液层并影响上皮细胞的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。

Description

TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 106 amino acids and having a total molecular mass of 12 kDa. TFF2 Human Recombinant includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds and was purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TFF-2 Human Recombinant 是单一的、非糖基化的多肽链，包含 106 个氨基酸，总分子量为 12 kDa。 TFF2 Human Recombinant 包含一个 40 个氨基酸的三叶基序，其中包含三个保守的分子内二硫键，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The TFF2 protein was lyophilized from 0.4μm filtered solution at a concentration of 1mg/mL containing 1x PBS pH-7.4.

Solubility

It is recommended to reconstitute the lyophilized TFF2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TFF2，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TFF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF2 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TFF2 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TFF2 应在 4°C 下储存 2-7 天，以备将来使用
-18°C。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Biological Activity

Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.

Amino acid sequence

EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKSVEDCHY.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

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  • TFF1 Human
  • TFF1 Human, His

ProSpec-TFF2 Human, His/CYT-611,Trefoil Factor-2 Human Recombinant, His Tag

ProSpec-TFF2 Human, His/CYT-611,Trefoil Factor-2 Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

TFF2 Human, His
Trefoil Factor-2 Human Recombinant, His Tag
CYT-611
5µg
20µg
1mg

Catalogue number

CYT-611

Synonyms

TFF-2, Spasmolytic polypeptide, Spasmolysin, SML1, Trefoil factor 2, SP, TFF2.

Introduction

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains.
TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠黏膜中表达的稳定分泌蛋白，可保护黏膜免受损伤、稳定黏液层并影响上皮细胞的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。

Description

TFF-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 13.2 kDa.
TFF2 Human Recombinant is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TFF-2 Human Recombinant 是一个单一的、非糖基化的多肽链，含有 116 个氨基酸，其中包括一个在 N 端融合的 10 个氨基酸的 His 标签，总分子量为 13.2 kDa。
TFF2 Human Recombinant 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

The TFF2 protein was lyophilized from 0.4μm filtered solution at a concentration of 0.5mg/ml containing 20mM Tris pH-7.5, and 20mM NaCl.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Lyophilized TFF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF2 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TFF2 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TFF2 应在 4°C 下储存 2-7 天，以备将来使用
-18°C。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHAS EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKSVEDCHY.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TFF1 Human
  • TFF3 Human
  • TFF2 Human
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  • TFF3 Human, His

ProSpec-TFF3 Human/CYT-005,Trefoil Factor-3 Human Recombinant

ProSpec-TFF3 Human/CYT-005,Trefoil Factor-3 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。

TFF3 Human

Trefoil Factor-3 Human Recombinant
CYT-005
5µg
20µg
1mg

Catalogue number

CYT-005

Synonyms

TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.

Introduction

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.
TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。 TFF3 相对通过表皮生长因子受体依赖性途径诱导纤毛发生并促进气道上皮纤毛细胞分化。 TFF3 过表达对于小鼠和人类肝细胞癌发生的进展至关重要。 TFF-3通常在肝细胞癌中表达，其表达与肿瘤分级相关。

Description

TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa.
TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TFF-3 Human Recombinant 是一种同型二聚体、非糖基化多肽链，含有 2 x 59 个氨基酸链，其中包括一个 40 个氨基酸的三叶基序，该基序含有 3 个保守的分子间二硫键，总分子量为 13.2kDa .
TFF-3 Human Recombinant 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized TFF3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TFF3，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TFF3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TFF3 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by RP-HPLC and -PAGE analysis.

Amino acid sequence

EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.

Biological Activity

The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10µg/ml, corresponding to a specific activity of > 100IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TFF2 Human
  • TFF3 Rat
  • TFF3 Human, His
  • TFF2 Human, His
  • TFF1 Human, His
  • TFF1 Human

ProSpec-TFF3 Human, His/CYT-616,Trefoil Factor-3 Human Recombinant, His Tag

ProSpec-TFF3 Human, His/CYT-616,Trefoil Factor-3 Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,加入去离子水至工作浓度约为 0.5 mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

TFF3 Human, His

Trefoil Factor-3 Human Recombinant, His Tag
CYT-616
5µg
20µg
1mg

Catalogue number

CYT-616

Synonyms

TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.

Introduction

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.
TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。 TFF3 相对通过表皮生长因子受体依赖性途径诱导纤毛发生并促进气道上皮纤毛细胞分化。 TFF3 过表达对于小鼠和人类肝细胞癌发生的进展至关重要。 TFF-3通常在肝细胞癌中表达，其表达与肿瘤分级相关。

Description

TFF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 7.82 kDa.
TFF3 Human Recombinant is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TFF3 Human Recombinant 是一个单一的、非糖基化的多肽链，含有 69 个氨基酸，其中包括一个在 N 端融合的 10 个氨基酸的 His 标签，总分子量为 7.82 kDa。
TFF3 Human Recombinant 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

White lyophilized powder.

Formulation

The TFF3 protein was lyophilized from 0.4μm filtered solution at a concentration of 0.5mg/mL containing 20mM Tris pH-7.5 and 150mM NaCl.

Solubility

Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
加入去离子水至工作浓度约为 0.5 mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TFF3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TFF3 应在 4°C 下储存 2-7 天，以备将来使用
-18°C。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as by -PAGE.

Amino acid sequence

MKHHHHHHAS EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TFF1 Human
  • TFF1 Human, His
  • TFF3 Rat
  • TFF3 Human
  • TFF2 Human
  • TFF2 Human, His

ProSpec-TFF3 Rat/CYT-781,Trefoil Factor-3 Rat Recombinant

ProSpec-TFF3 Rat/CYT-781,Trefoil Factor-3 Rat Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。

TFF3 Rat

Trefoil Factor-3 Rat Recombinant
CYT-781
2µg
10µg
1mg

Catalogue number

CYT-781

Synonyms

Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1.B, rP1.B, Tff3, Itf.

Introduction

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.
TFF 家族的蛋白质的特征在于获得至少 1 个三叶基序拷贝，这是一个包含 3 个保守二硫键的 40 个氨基酸结构域。 三叶因子是在胃肠道粘膜中表达的稳定分泌蛋白，可保护粘膜免受损伤，稳定粘液层并影响上皮的愈合。TFF2 抑制胃酸运动和分泌。 TFF2 通过与碳水化合物侧链的相互作用稳定粘液凝胶中的糖蛋白。 TFF3 相对通过表皮生长因子受体依赖性途径诱导纤毛发生并促进气道上皮纤毛细胞分化。 TFF3 过表达对于小鼠和人类肝细胞癌发生的进展至关重要。 TFF-3通常在肝细胞癌中表达，其表达与肿瘤分级相关。

Description

The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a.a C-terminal fusion of Flag-Tag . The TFF3 Rat produced in E.coli, is 7.7kDa protein containing a total of 68 amino acid residues.
Trefoil Factor-3 Rat 作为重组蛋白构建，具有 9 a.a C 末端融合的 Flag-Tag。 在大肠杆菌中产生的 TFF3 大鼠是 7.7kDa 的蛋白质，总共含有 68 个氨基酸残基。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

TFF3 protein filtered and lyophilized in 0.5mg/ml in 20mM TRIS and 50mM NaCl, pH 7.5.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TFF3 Human
  • TFF3 Human, His

ProSpec-TSLP Human/CYT-572,Thymic Stromal Lymphopoietin Human Recombinant

ProSpec-TSLP Human/CYT-572,Thymic Stromal Lymphopoietin Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TSLP 蛋白是一种造血细胞因子，通过由胸腺基质淋巴细胞生成素受体和白细胞介素 7 受体α链组成的异二聚体受体复合物发出信号。 TSLP 影响骨髓细胞，从而诱导 T 细胞吸引趋化因子从单核细胞中释放并增加 CD11c 树突状细胞的生长。 TSLP主要在心脏、肝脏和前列腺中表达。

TSLP Human
Thymic Stromal Lymphopoietin Human Recombinant
CYT-572
2µg
10µg
1mg

Catalogue number

CYT-572

Synonyms

Thymic Stromal Lymphopoietin, TSLP.

Introduction

TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-alpha whereas down-regulating Interleukin-10 and IFN-gamma play a role in the initiation of allergic inflammation.
TSLP 蛋白是一种造血细胞因子，通过由胸腺基质淋巴细胞生成素受体和白细胞介素 7 受体α链组成的异二聚体受体复合物发出信号。 TSLP 影响骨髓细胞，从而诱导 T 细胞吸引趋化因子从单核细胞中释放并增加 CD11c 树突状细胞的生长。 TSLP主要在心脏、肝脏和前列腺中表达。 TSLP 的生物活性与 IL-7 相关，并与由 IL-7 受体 α 链和 TSLPR 组成的异二聚体受体复合物结合。与 IL-7 类似，TSLP 增强 STAT3 和 STAT5 的磷酸化，但使用不包括 JAK 的激酶进行激活。 TSLP 诱导 T 细胞吸引趋化因子如 TARC 和 MDC 从单核细胞中释放并触发 CD11c 树突状细胞。 TSLP 激活的树突状细胞启动幼稚 T 细胞以制造促过敏细胞因子，如 Iinterleukin-4、Interleukin-5、Interleukin-13 和 TNF-α，而下调 Interleukin-10 和 IFN-γ 在过敏的起始中发挥作用炎。

Description

TSLP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa.
The TSLP is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TSLP Human Recombinant 是单一的非糖基化多肽链，包含 132 个氨基酸，分子量为 15 kDa。
TSLP 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution contains 130mM NaCl, and 20mM sodium phosphate, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized TSLP in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TSLP，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TSLP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TSLP should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TSLP 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后 TSLP 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ.

Biological Activity

The ED50 is determined by the ability to induce proliferation of human Interleukin-7 receptor alpha & human TSLP Receptor co-transfected with mouse BaF3 cells which was found to be less than 0.3ng/ml.

ED50 由诱导共转染小鼠 BaF3 细胞的人白介素 7 受体 α 和人 TSLP 受体增殖的能力确定，发现低于 0.3ng/ml。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TSLPR Human
  • TSLPR Human, Sf9
  • TSLP Human, His
  • TSLP Mouse

ProSpec-TSLP Human, His/CYT-836,Thymic Stromal Lymphopoietin Human Recombinant, His Tag

ProSpec-TSLP Human, His/CYT-836,Thymic Stromal Lymphopoietin Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在大肠杆菌中产生的 TSLP Human Recombinant 是一种单一的、非糖基化的多肽链，含有 141 个氨基酸，包括 N 端的 10 个氨基酸的 His 标签。 总计算分子量为 16.2kDa。

TSLP Human, His
Thymic Stromal Lymphopoietin Human Recombinant, His Tag
CYT-836
2µg
10µg
1mg

Catalogue number

CYT-836

Synonyms

Thymic Stromal Lymphopoietin, TSLP.

Introduction

TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-alpha whereas down-regulating Interleukin-10 and IFN-gamma play a role in the initiation of allergic inflammation.
TSLP 蛋白是一种造血细胞因子，通过由胸腺基质淋巴细胞生成素受体和白细胞介素 7 受体α链组成的异二聚体受体复合物发出信号。 TSLP 影响骨髓细胞，从而诱导 T 细胞吸引趋化因子从单核细胞中释放并增加 CD11c 树突状细胞的生长。 TSLP主要在心脏、肝脏和前列腺中表达。 TSLP 的生物活性与 IL-7 相关，并与由 IL-7 受体 α 链和 TSLPR 组成的异二聚体受体复合物结合。与 IL-7 类似，TSLP 增强 STAT3 和 STAT5 的磷酸化，但使用不包括 JAK 的激酶进行激活。 TSLP 诱导 T 细胞吸引趋化因子如 TARC 和 MDC 从单核细胞中释放并触发 CD11c 树突状细胞。 TSLP 激活的树突状细胞启动幼稚 T 细胞以制造促过敏细胞因子，如 Iinterleukin-4、Interleukin-5、Interleukin-13 和 TNF-α，而下调 Interleukin-10 和 IFN-γ 在过敏的起始中发挥作用炎。

Description

TSLP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 16.2kDa.
在大肠杆菌中产生的 TSLP Human Recombinant 是一种单一的、非糖基化的多肽链，含有 141 个氨基酸，包括 N 端的 10 个氨基酸的 His 标签。 总计算分子量为 16.2kDa。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

TSLP was filtered and lyophilized in phosphate buffered saline.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. TSLP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 TSLP 不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间。

Amino acid sequence

MKHHHHHHASYDFTNCDFEK IKAAYLSTIS KDLITYMSGT KSTEFNNTVS CSNRPHCLTE IQSLTFNPTA GCASLAKEMF AMKTKAALAI WCPGYSETQI NATQAMKKRR KRKVTTNKCL EQVSQLQGLW RRFNRPLLKQ Q.

Purity

Greater than 95.0% as determined by -PAGE.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TSLPR Human, Sf9
  • TSLP Human
  • TSLP Mouse

ProSpec-TSLP Mouse/CYT-1135,Thymic Stromal Lymphopoietin Mouse Recombinant

ProSpec-TSLP Mouse/CYT-1135,Thymic Stromal Lymphopoietin Mouse Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

TSLP Mouse
Thymic Stromal Lymphopoietin Mouse Recombinant
CYT-1135
1µg
5µg
50µg

Catalogue number

CYT-1135

Synonyms

Thymic Stromal Lymphopoietin, TSLP.

Introduction

TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-alpha whereas down-regulating Interleukin-10 and IFN-gamma play a role in the initiation of allergic inflammation.

TSLP 蛋白是一种造血细胞因子，通过由胸腺基质淋巴细胞生成素受体和白细胞介素 7 受体α链组成的异二聚体受体复合物发出信号。 TSLP 影响骨髓细胞，从而诱导 T 细胞吸引趋化因子从单核细胞中释放并增加 CD11c 树突状细胞的生长。 TSLP主要在心脏、肝脏和前列腺中表达。 TSLP 的生物活性与 IL-7 相关，并与由 IL-7 受体 α 链和 TSLPR 组成的异二聚体受体复合物结合。与 IL-7 类似，TSLP 增强 STAT3 和 STAT5 的磷酸化，但使用不包括 JAK 的激酶进行激活。 TSLP 诱导 T 细胞吸引趋化因子如 TARC 和 MDC 从单核细胞中释放并触发 CD11c 树突状细胞。 TSLP 激活的树突状细胞启动幼稚 T 细胞以制造促过敏细胞因子，如 Iinterleukin-4、Interleukin-5、Interleukin-13 和 TNF-α，而下调 Interleukin-10 和 IFN-γ 在过敏的起始中发挥作用炎。

Description

TSLP Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15.0kDa.
TSLP is fused to a 29 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在杆状病毒中产生的 TSLP 小鼠重组体是一条含有 130 个氨基酸的单糖基化多肽链，分子量为 15.0kDa。
TSLP 在 C 末端与 29 个氨基酸的 His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The TSLP solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ADPYNFSNCN FTSITKIYCN IIFHDLTGDL KGAKFEQIED CESKPACLLK IEYYTLNPIP
GCPSLPDKTF ARRTREALND HCPGYPETER NDGTQEMAQE VQNICLNQTS QILRLWYSFM QSPEHHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • TSLP Human
  • TSLP Human, His

ProSpec-TSLPR Human/CYT-742,Thymic Stromal Lymphopoietin Receptor Human Recombinant

ProSpec-TSLPR Human/CYT-742,Thymic Stromal Lymphopoietin Receptor Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TSLPR protein solution contains 20mM Tris-HCl buffer, , 0.4M UREA and 10% glycerol.

TSLPR Human
Thymic Stromal Lymphopoietin Receptor Human Recombinant
CYT-742
5µg
20µg
1mg

Catalogue number

CYT-742

Synonyms

CRL2, CRLF2Y, Cytokine receptor-like factor 2, Cytokine receptor-like 2, IL-XR, ILXR.

Introduction

Thymic Stromal Lymphopoietin Receptor is a member of the type I cytokine receptor family. TSLPR alongside the interleukin 7 receptor activate STAT3, STAT5, and JAK2 pathways, which is regulated processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding dissimilar isoforms have been found for this gene. TSLPR is expressed in heart, skeletal muscle, kidney and adult and fetal liver.
胸腺基质淋巴细胞生成素受体是 I 型细胞因子受体家族的成员。 TSLPR 与白细胞介素 7 受体一起激活 STAT3、STAT5 和 JAK2 通路，这是细胞增殖和造血系统发育等调节过程。 已经为该基因发现了两个编码不同同种型的转录变体。 TSLPR 在心脏、骨骼肌、肾脏以及成人和胎儿肝脏中表达。

Description

TSLPR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 232 amino acids and having a molecular mass of 26.6kDa.
TSLPR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TSLPR Human Recombinant 是一条单一的、非糖基化的多肽链，包含 232 个氨基酸，分子量为 26.6kDa。
TSLPR 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

TSLPR protein solution contains 20mM Tris-HCl buffer, , 0.4M UREA and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSQGGAAEG VQIQIIYFNL ETVQVTWNAS KYSRTNLTFH YRFNGDEAYD QCTNYLLQEG HTSGCLLDAE QRDDILYFSI RNGTHPVFTA SRWMVYYLKP SSPKHVRFSW HQDAVTVTCS DLSYGDLLYE VQYRSPFDTE WQSKQENTCN VTIEGLDAEK CYSFWVRVKA MEDVYGPDTY PSDWSEVTCW QRGEIRDACA ETPTPPKPKL SK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TSLPR Human, Sf9
  • TSLPR Antibody
  • TSLP Human

ProSpec-TSLPR Human, Sf9/CYT-995,Thymic Stromal Lymphopoietin Receptor Human Recombinant, Sf9

ProSpec-TSLPR Human, Sf9/CYT-995,Thymic Stromal Lymphopoietin Receptor Human Recombinant, Sf9,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Cytokine receptor-like factor 2, CRLF2, Cytokine receptor-like factor 2, Cytokine receptor-like 2, IL-XR, Thymic stromal lymphopoietin protein receptor, TSLP receptor, CRL2, ILXR, TSLPR, Cytokine receptor-like factor 2 isoform 1, CRLF2Y.

TSLPR Human, Sf9
Thymic Stromal Lymphopoietin Receptor Human Recombinant, Sf9
CYT-995
2µg
10µg
1mg

Catalogue number

CYT-995

Synonyms

Cytokine receptor-like factor 2, CRLF2, Cytokine receptor-like factor 2, Cytokine receptor-like 2, IL-XR, Thymic stromal lymphopoietin protein receptor, TSLP receptor, CRL2, ILXR, TSLPR, Cytokine receptor-like factor 2 isoform 1, CRLF2Y.

Introduction

Thymic Stromal Lymphopoietin Receptor is a member of the type I cytokine receptor family. TSLPR alongside the interleukin 7 receptor activate STAT3, STAT5, and JAK2 pathways, which is regulated processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding dissimilar isoforms have been found for this gene. TSLPR is expressed in heart, skeletal muscle, kidney and adult and fetal liver.
胸腺基质淋巴细胞生成素受体是 I 型细胞因子受体家族的成员。 TSLPR 与白细胞介素 7 受体一起激活 STAT3、STAT5 和 JAK2 通路，这是细胞增殖和造血系统发育等调节过程。 已经为该基因发现了两个编码不同同种型的转录变体。 TSLPR 在心脏、骨骼肌、肾脏以及成人和胎儿肝脏中表达。

Description

TSLPR Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 218 amino acids and having a molecular mass of 25.2kDa .
TSLPR is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 TSLPR Human Recombinant 是单条糖基化多肽链，包含 218 个氨基酸，分子量为 25.2kDa。
TSLPR 在 C 端与 6 个氨基酸的 His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TSLPR protein solution containing Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ADPQGGAAEG VQIQIIYFNL ETVQVTWNAS KYSRTNLTFH YRFNGDEAYD QCTNYLLQEG HTSGCLLDAE QRDDILYFSI RNGTHPVFTA SRWMVYYLKP SSPKHVRFSW HQDAVTVTCS DLSYGDLLYE VQYRSPFDTE WQSKQENTCN VTIEGLDAEK CYSFWVRVKA MEDVYGPDTY PSDWSEVTCW QRGEIRDACA ETPTPPKPKL SKHHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TSLPR Human
  • TSLP Human, His
  • TSLPR Antibody
  • TSLP Human

ProSpec-TNFRSF10A Human/CYT-1043,TRAIL Receptor-1 Human Recombinant

ProSpec-TNFRSF10A Human/CYT-1043,TRAIL Receptor-1 Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNFRSF10A, TNF Receptor Superfamily Member 10a, Tumor Necrosis Factor Receptor Superfamily, Member 10a, TNF-Related Apoptosis-Inducing Ligand Receptor 1, Death Receptor 4, TRAIL Receptor 1, TRAIL-R1, TRAILR1, APO2, DR4, Tumor Necrosis Factor Receptor Superfamily Member 10a Variant 2, Tumor Necrosis Factor Receptor Superfamily Member 10A, Cytotoxic TRAIL Receptor, CD261 Antigen, TRAILR-1, CD261.

TNFRSF10A Human

TRAIL Receptor-1 Human Recombinant
CYT-1043
2µg
10µg
1mg

Catalogue number

CYT-1043

Synonyms

TNFRSF10A, TNF Receptor Superfamily Member 10a, Tumor Necrosis Factor Receptor Superfamily, Member 10a, TNF-Related Apoptosis-Inducing Ligand Receptor 1, Death Receptor 4, TRAIL Receptor 1, TRAIL-R1, TRAILR1, APO2, DR4, Tumor Necrosis Factor Receptor Superfamily Member 10a Variant 2, Tumor Necrosis Factor Receptor Superfamily Member 10A, Cytotoxic TRAIL Receptor, CD261 Antigen, TRAILR-1, CD261.

Introduction

Human TNFRSF10A is a type 1 transmembrane protein in the TNF R superfamily. TNFRSF10A is not expressed in rodents. The trimeric ligand Trail binds TNFRSF10A and induces apoptosis, and recombinant, soluble forms of the receptor inhibit Trail-induced apoptosis. TNFRSF10A is expressed generally in damaged, infected, and malignant cells. TNFRSF10A functions in immune surveillance, inducing apoptosis in cancer cells but not normal cells.

人 TNFRSF10A 是 TNF R 超家族中的 1 型跨膜蛋白。 TNFRSF10A 在啮齿动物中不表达。 三聚体配体 Trail 结合 TNFRSF10A 并诱导细胞凋亡，而重组的可溶性受体形式可抑制 Trail 诱导的细胞凋亡。 TNFRSF10A 通常在受损、感染和恶性细胞中表达。 TNFRSF10A 在免疫监视中起作用，诱导癌细胞而非正常细胞的凋亡。

Description

TNFRSF10A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 224 amino acids and having a molecular mass of 23.9kDa. TNFRSF10A is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNFRSF10A 是单条糖基化多肽链，包含 224 个氨基酸，分子量为 23.9kDa。 TNFRSF10A 在 C 末端带有 6 个氨基酸的 His 标签，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

TNFRSF10A protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。

对于长期储存，建议添加载体蛋白。

避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ASGTEAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSEHPG ACNRCTEGVG YTNASNNLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSRGCPR GMVKVKDCTP WSDIECVHKE SGNGHNLEHH HHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TNFRSF10A Human Fc

ProSpec-LTBR Human/CYT-853

ProSpec-LTBR Human,ProSpec CYT-853,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Lymphotoxin Beta Receptor，也称为 LTBR，参与淋巴和其他器官发育、脂质代谢、免疫反应和程序性细胞死亡过程中的信号传导。 此外，这种受体的活性与致癌作用有关。 对于 LTBR，已观察到编码多种同种型的选择性剪接转录变体。

LTBR Human

Lymphotoxin Beta Receptor Human Recombinant
CYT-853
ProSpec-LTBR Human/CYT-853 2µg
ProSpec-LTBR Human/CYT-853 10µg
ProSpec-LTBR Human/CYT-853 1mg

Catalogue number

CYT-853

Synonyms

Lymphotoxin Beta Receptor , TNFCR,Tumor Necrosis Factor Receptor 2-Related Protein,Tumor Necrosis Factor Receptor Type III, Tumor Necrosis Factor C Receptor,D12S370, TNFRSF3,TNFR3,Tumor Necrosis Factor Receptor Superfamily Member 3,Lymphotoxin-Beta Receptor,Lymphotoxin B Receptor, LT-BETA-R,TNF-R-III, TNFR2-RP, TNF-RIII,TNFR-III, TNFR-RP, CD18, LTBR.

Introduction

Lymphotoxin Beta Receptor, also known as LTBR takes part in signaling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. In addition, the activity of this receptor has been associated to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for LTBR.
Lymphotoxin Beta Receptor，也称为 LTBR，参与淋巴和其他器官发育、脂质代谢、免疫反应和程序性细胞死亡过程中的信号传导。 此外，这种受体的活性与致癌作用有关。 对于 LTBR，已观察到编码多种同种型的选择性剪接转录变体。

Description

LTBR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 224 amino acids and having a molecular mass of 24.6kDa.
LTBR is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 LTBR Human Recombinant 是单一的非糖基化多肽链，包含 224 个氨基酸，分子量为 24.6kDa。
LTBR 在 N 端与 24 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

LTBR protein solution containing PBS buffer 10% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMSQPQAV PPYASENQTC RDQEKEYYEP QHRICCSRCP PGTYVSAKCS RIRDTVCATC AENSYNEHWN YLTICQLCRP CDPVMGLEEI APCTSKRKTQ CRCQPGMFCA AWALECTHCE LLSDCPPGTE AELKDEVGKG NNHCVPCKAG HFQNTSSPSA RCQPHTRCEN QGLVEAAPGT AQSDTTCKNP LEPLPPEMSG TMLM

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-Mouse OX40L/CYT-1191

ProSpec-Mouse OX40L,ProSpec CYT-1191,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,肿瘤坏死因子配体超家族成员 4、OX40 配体、OX40L、OX-40L、CD252、Tnfsf4、Ox40l、Txgp1l、gp3、OX4、Ath-1、Ath1、CD134L、gp34、Tnlg2b、Txgp1l

Mouse OX40L

OX40 Ligand Mouse Recombinant
CYT-1191
ProSpec-Mouse OX40L/CYT-1191 2µg
ProSpec-Mouse OX40L/CYT-1191 10µg
ProSpec-Mouse OX40L/CYT-1191 100µg

Catalogue number

CYT-1191

Synonyms

Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, OX-40L, CD252, Tnfsf4, Ox40l, Txgp1l, gp3, OX4, Ath-1, Ath1, CD134L, gp34, Tnlg2b, Txgp1l

肿瘤坏死因子配体超家族成员 4、OX40 配体、OX40L、OX-40L、CD252、Tnfsf4、Ox40l、Txgp1l、gp3、OX4、Ath-1、Ath1、CD134L、gp34、Tnlg2b、Txgp1l

Introduction

OX40 Ligandis a part of the tumor necrosis factor receptor family. OX40L receptor is OX40 which is expressed predominantly on activated CD4+ T cells.OX40 Ligandis expressed on various antigen-presenting cells such asmacrophages, DC2sand activated Blymphocytes. Expression of OX40 is momentary following engagement of T cell receptors. The ligation of OX40-OX40L stimulates proliferation anddifferentiation of activated B cells,increases immunoglobulin secretion and is also a sourceof survival signal for T cells and enables the development of memory T cells.

OX40 配体是肿瘤坏死因子受体家族的一部分。 OX40L受体是主要在活化的CD4+ T细胞上表达的OX40。OX40配体在各种抗原呈递细胞如巨噬细胞、DC2和活化的淋巴细胞上表达。 OX40 的表达是 T 细胞受体结合后的瞬间。 OX40-OX40L的连接刺激活化B细胞的增殖和分化，增加免疫球蛋白分泌，也是T细胞生存信号的来源，使记忆T细胞发育成为可能。

Description

OX40L Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 17.7kDa.
OX40L  is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 OX40L 小鼠是一条糖基化多肽链，包含 157 个氨基酸，分子量为 17.7kDa。
OX40L 在 C 端与 6 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The OX40L solution contains 10% Glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Biological Activity

Determined by its binding ability in a functional ELISA with Human TNFRSF4 .

Amino acid sequence

ADPSSSPAKD PPIQRLRGAV TRCEDGQLFI SSYKNEYQTM EVQNNSVVIK CDGLYIIYLK GSFFQEVKID LHFREDHNPI SIPMLNDGRR IVFTVVASLA FKDKVYLTVN APDTLCEHLQ INDGELIVVQ LTPGYCAPEG SYHSTVNQVP LHHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-RANK Mouse/CYT-1161,RANK Mouse Recombinant

ProSpec-RANK Mouse/CYT-1161,RANK Mouse Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, ODFR, Receptor activator of NF-KB, activator of NFKB, FEO, OFE, OSTS, PDB2, RANK, RANKLOH18CR1, CD265, CD265 antigen, OPTB7, TRANCER, LOH18CR1, receptor activator of nuclear factor-kappa B.

RANK Mouse
RANK Mouse Recombinant
CYT-1161
2µg
10µg
1mg

Catalogue number

CYT-1161

Synonyms

Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, ODFR, Receptor activator of NF-KB, activator of NFKB, FEO, OFE, OSTS, PDB2, RANK, RANKLOH18CR1, CD265, CD265 antigen, OPTB7, TRANCER, LOH18CR1, receptor activator of nuclear factor-kappa B.

Introduction

Tumor necrosis factor receptor superfamily member 11A or TNFRSF11A or RANK, is a protein, part of the tumor necrosis factor receptor group of proteins. RANK acts as the receptor of RANK-Ligand and regulates osteoclast activation & differentiation. RANK protein has been linked to activation of NF-kappa B & c-jun N-terminal kinase, enhancement of T cell growth, bone remodeling and repair, dendritic cell function, lymph node development, immune cell function, mammary gland development  & thermal regulation.

肿瘤坏死因子受体超家族成员11A或TNFRSF11A或RANK，是一种蛋白质，是肿瘤坏死因子受体蛋白组的一部分。 RANK 作为 RANK-Ligand 的受体并调节破骨细胞的活化和分化。 RANK 蛋白与激活 NF-kappa B 和 c-jun N-末端激酶、增强 T 细胞生长、骨重塑和修复、树突细胞功能、淋巴结发育、免疫细胞功能、乳腺发育和热调节有关 .

Description

RANK Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 426 amino acids and having a molecular mass of 47.5kDa.
RANK is fused to a 242 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在杆状病毒中产生的RANK小鼠重组体是一条含有426个氨基酸的单糖基化多肽链，分子量为47.5kDa。
RANK 在 C 端与 242 个氨基酸的 hIgG-His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

RANK protein contains 10% glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ADLVTPPCTQ ERHYEHLGRC CSRCEPGKYL SSKCTPT VCLPCGPDEY LDTWNEEDKC LLHKVCDAGK ALVAVDPGNH TAPRRCACTA GYHWNSDCEC CRRNTECAPG FGAQHPLQLN KDTVCTPCLL GFFSDVFSST DKCKPWTNCT LLGKLEAHQG TTESDVVCSS SMTLRRPPKE AQAYLPSLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI VEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • RANK Human
  • RANK Human, Sf9

ProSpec-TACI Human, Sf9/CYT-982,Tumor Necrosis Factor Receptor 13B Human Recombinant, Sf9

ProSpec-TACI Human, Sf9/CYT-982,Tumor Necrosis Factor Receptor 13B Human Recombinant, Sf9,肿瘤坏死因子受体超家族成员 13B、肿瘤坏死因子受体超家族成员 13B、跨膜激活因子和 CAML 相互作用因子、TACI、肿瘤坏死因子受体 13B、CD267 抗原、TNFRSF14B、CD267、CVID2、IGAD2、CVID、RYZN。

TACI Human, Sf9
Tumor Necrosis Factor Receptor 13B Human Recombinant, Sf9
CYT-982
2µg
10µg
1mg

Catalogue number

CYT-982

Synonyms

Tumor Necrosis Factor Receptor Superfamily Member 13B, Tumor Necrosis Factor Receptor Superfamily, Member 13B, Transmembrane Activator And CAML Interactor ,TACI , Tumor Necrosis Factor Receptor 13B, CD267 Antigen, TNFRSF14B, CD267, CVID2, IGAD2, CVID, RYZN.
肿瘤坏死因子受体超家族成员 13B、肿瘤坏死因子受体超家族成员 13B、跨膜激活因子和 CAML 相互作用因子、TACI、肿瘤坏死因子受体 13B、CD267 抗原、TNFRSF14B、CD267、CVID2、IGAD2、CVID、RYZN。

Introduction

TNFRSF13B is a transmembrane receptor protein found predominantly on the surface of B cells . TACI was at first discovered owing to its ability to interact with calcium-modulator and cyclophilin ligand . Later on, it was found that TACI plays a key role in humoral immunity by interacting with two members of the TNF family. Also, TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis.
TNFRSF13B 是一种跨膜受体蛋白，主要存在于 B 细胞表面。 最初发现 TACI 是因为它能够与钙调节剂和亲环蛋白配体相互作用。 后来发现TACI通过与TNF家族的两个成员相互作用在体液免疫中起关键作用。 此外，TACI 控制不依赖 T 细胞的 B 细胞抗体反应、同种型转换和 B 细胞稳态。

Description

TACI produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 407 amino acids and having a molecular mass of 45.8kDa . TACI is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

 

 

Source

Sf9, Insect cells.

Physical Appearance

Sterile filtered colorless soltion.u

Formulation

TACI a protein solution contains Phosphate Buffered Saline , 30% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ADLMSGLGRS RRGGRSRVDQ EERFPQGLWT GVAMRSCPEE QYWDPLLGTC MSCKTICNHQ SQRTCAAFCR SLSCRKEQGK FYDHLLRDCI SCASICGQHP KQCAYFCENK LRSPVNLPPE LRRQRSGEVE NNSDNSGRYQ GLEHRGSEAS PALPGLKLSA DQVALVYSLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TACI Human, His
  • TACI Human

ProSpec-TACI Human/CYT-819,Tumor Necrosis Factor Receptor 13B Human Recombinant

ProSpec-TACI Human/CYT-819,Tumor Necrosis Factor Receptor 13B Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,与标准相比具有完全的生物活性。 生物活性取决于其阻断人 BAFF 诱导的 T2B 细胞存活的能力，使用 1.0-3.0 µg/ml 的浓度范围，对应于 334-1000 IU/mg 的比活性。

TACI Human
Tumor Necrosis Factor Receptor 13B Human Recombinant
CYT-819
5µg
20µg
1mg

Catalogue number

CYT-819

Synonyms

CD267, CVID, CVID2, TACI, TNFRSF14B, Tumor necrosis factor receptor superfamily, member 13B, Tumor necrosis factor receptor superfamily, member 13B, isoform CRA_a, TNFRSF13B.

Introduction

TNFRSF13B is a transmembrane receptor protein found predominantly on the surface of B cells . TACI was at first discovered owing to its ability to interact with calcium-modulator and cyclophilin ligand . Later on, it was found that TACI plays a key role in humoral immunity by interacting with two members of the TNF family. Also, TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis.

Description

TACI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 160 amino acids and having a molecular mass of 18.0kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TACI protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized TACI in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TACI，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TACI although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TACI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TACI 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TACI 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MSGLGRSRRG GRSRVDQEER FPQGLWTGVA MRSCPEEQYW DPLLGTCMSC KTICNHQSQR TCAAFCRSLS CRKEQGKFYD HLLRDCISCA SICGQHPKQC AYFCENKLRS PVNLPPELRR QRSGEVENNS DNSGRYQGLE HRGSEASPAL PGLKLSADQV

Biological Activity

Fully biologically active when compared to standard. The biological activity is determined by its ability to block human BAFF induced T2B cell survival using a concentration range of 1.0-3.0 µg/ml corresponding to a specific activity of 334-1000 IU/mg.
与标准相比具有完全的生物活性。 生物活性取决于其阻断人 BAFF 诱导的 T2B 细胞存活的能力，使用 1.0-3.0 µg/ml 的浓度范围，对应于 334-1000 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TACI Human, His
  • TACI Human, Sf9

ProSpec-TACI Human, His/CYT-032,Tumor Necrosis Factor Receptor 13B Human Recombinant, His Tag

ProSpec-TACI Human, His/CYT-032,Tumor Necrosis Factor Receptor 13B Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNFRSF13B 是一种跨膜受体蛋白，主要存在于 B 细胞表面。 最初发现 TACI 是因为它能够与钙调节剂和亲环蛋白配体相互作用。 后来发现TACI通过与TNF家族的两个成员相互作用在体液免疫中起关键作用。 此外，TACI 控制不依赖 T 细胞的 B 细胞抗体反应、同种型转换和 B 细胞稳态。

TACI Human, His
Tumor Necrosis Factor Receptor 13B Human Recombinant, His Tag
CYT-032
5µg
20µg
1mg

Catalogue number

CYT-032

Synonyms

CD267, CVID, CVID2, TACI, TNFRSF14B, Tumor necrosis factor receptor superfamily, member 13B, Tumor necrosis factor receptor superfamily, member 13B, isoform CRA_a, TNFRSF13B.

Introduction

TNFRSF13B is a transmembrane receptor protein found predominantly on the surface of B cells . TACI was at first discovered owing to its ability to interact with calcium-modulator and cyclophilin ligand . Later on, it was found that TACI plays a key role in humoral immunity by interacting with two members of the TNF family. Also, TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis.
TNFRSF13B 是一种跨膜受体蛋白，主要存在于 B 细胞表面。 最初发现 TACI 是因为它能够与钙调节剂和亲环蛋白配体相互作用。 后来发现TACI通过与TNF家族的两个成员相互作用在体液免疫中起关键作用。 此外，TACI 控制不依赖 T 细胞的 B 细胞抗体反应、同种型转换和 B 细胞稳态。

Description

TACI Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of 20.9kDa. The TACI is purified by proprietary chromatographic techniques.
TACI Human Recombinant 在 N 末端融合了 23 个氨基酸的 His 标签，在大肠杆菌中产生，是一个单一的、非糖基化的多肽链，包含 188 个氨基酸，分子量为 20.9kDa。 TACI 通过专有的色谱技术进行纯化。

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The TACI solution contains 20mM Tris-HCl buffer , 10% glycerol and 0.4M Urea.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMSGLGRS RRGGRSRVDQ EERFPQGLWT GVAMRSCPEE QYWDPLLGTC MSCKTICNHQ SQRTCAAFCR SLSCRKEQGK FYDHLLRDCI SCASICGQHP KQCAYFCENK LRSPVNLPPE LRRQRSGEVE NNSDNSGRYQ GLEHRGSEAS PALPGLKLSA DQVALVYS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • TACI Human
  • TACI Human, Sf9

ProSpec-TNF a Canine/CYT-140,Tumor Necrosis Factor-Alpha Canine Recombinant

ProSpec-TNF a Canine/CYT-140,Tumor Necrosis Factor-Alpha Canine Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Filtered and lyophilized from a concentrated solution in 1×PBS, pH7.4.

TNF a Canine

Tumor Necrosis Factor-Alpha Canine Recombinant
CYT-140
5µg
20µg
1mg

Catalogue number

CYT-140

Synonyms

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNF-a Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 17.3 kDa.
The TNF-a is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNF-a Canine Recombinant 是一条单一的、非糖基化的多肽链，包含 157 个氨基酸，分子量为 17.3 kDa。
TNF-a 是通过专有的色谱技术纯化的。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from a concentrated solution in 1×PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的肿瘤坏死因子-α，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子-a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后的 TNF-a 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

VKSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLIV PSDGLYLIYS QVLFKGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGTE AKPWYEPIYL GGVFQLEKGD RLSAEINLPN YLDFAESGQV YFGIIAL.

Biological Activity

The Specific Activity is >3.3×105 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNF a Mutant Human
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  • TNF a Human, Sf9
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  • TNF a Human, His
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ProSpec-TNF a Human, HEK/CYT-114,Tumor Necrosis Factor-alpha Human Recombinant, HEK

ProSpec-TNF a Human, HEK/CYT-114,Tumor Necrosis Factor-alpha Human Recombinant, HEK,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D and is typically 0.05-0.5ng/ml.

TNF a Human, HEK
Tumor Necrosis Factor-alpha Human Recombinant, HEK
CYT-114
2µg
10µg
1mg

Catalogue number

CYT-114

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNF-a Human Recombinant produced in HEK cells is a glycosylated non-disulfide linked homotrimer, containing 157 and having total Mw of 17kDa.
The TNF-a is purified by proprietary chromatographic techniques.
在 HEK 细胞中产生的 TNF-a Human Recombinant 是一种糖基化的非二硫键连接的同源三聚体，含有 157 个，总 Mw 为 17kDa。
TNF-a 是通过专有的色谱技术纯化的。

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The TNF-a protein was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized TNF-a in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 TNF-a 溶解在不低于 100μg/ml 的无菌水中，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TNF-a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后的 TNF-a 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95% as obsereved by -PAGE.

Biological Activity

The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D and is typically 0.05-0.5ng/ml.

Amino acid sequence

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLI
YSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYL
GGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNF alpha human
  • TNF a Human, Sf9
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  • TNF a Human, His
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  • TNF a Antibody
  • TNF a Mouse
  • TNF a Rat, His
  • TNF a Canine
  • TNF a Mutant Human
  • Recombinant TNF-a Antibody
  • TNFA Bovine

ProSpec-TNF a Human, His/CYT-494,Tumor Necrosis Factor-Alpha Human Recombinant, His Tag

ProSpec-TNF a Human, His/CYT-494,Tumor Necrosis Factor-Alpha Human Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

TNF a Human, His
Tumor Necrosis Factor-Alpha Human Recombinant, His Tag
CYT-494
10µg
50µg
1mg

Catalogue number

CYT-494

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

Tumor Necrosis Factor-α Human Recombinant His produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids fragment and having a molecular mass of 18.3kDa with an N-terminal hexahistidine tag. The TNF-alpha His is purified by standard chromatographic techniques.

Tumor Necrosis Factor-α Human Recombinant His 在大肠杆菌中产生，是一种单一的、非糖基化的多肽链，包含 164 个氨基酸片段，分子量为 18.3kDa，带有 N 端六组氨酸标签。 TNF-α His 通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Solubility

It is recommended to reconstitute the lyophilized TNF-α in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 TNF-α，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TNF-α although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-α should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by -PAGE and HPLC analyses.

Amino acid sequence

MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL.

Biological Activity

The ED50 was determined in the presence of actinomycin D by a cytotoxicity assay using murine L929 cells is <0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF a Human, Sf9/CYT-903,Tumor Necrosis Factor-alpha Human Recombinant, Sf9

ProSpec-TNF a Human, Sf9/CYT-903,Tumor Necrosis Factor-alpha Human Recombinant, Sf9,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor Necrosis Factor, TNFA, Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin, TNF-Alpha, TNFSF2, TNF-A, Tumor Necrosis Factor , Tumor Necrosis Factor-Alpha, TNF, Macrophage-Derived, TNF, Monocyte-Derived, TNF Superfamily, APC1 Protein, Member 2, DIF, Tumor necrosis factor.

TNF a Human, Sf9
Tumor Necrosis Factor-alpha Human Recombinant, Sf9
CYT-903
2µg
10µg
1mg

Catalogue number

CYT-903

Synonyms

Tumor Necrosis Factor, TNFA, Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin, TNF-Alpha, TNFSF2, TNF-A, Tumor Necrosis Factor , Tumor Necrosis Factor-Alpha, TNF, Macrophage-Derived, TNF, Monocyte-Derived, TNF Superfamily, APC1 Protein, Member 2, DIF, Tumor necrosis factor.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNF a produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 163 amino acids and having a molecular mass of 18.1kDa.

TNF a is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 TNF a 是单条糖基化多肽链，含有 163 个氨基酸，分子量为 18.1kDa。

TNF a 在 C 末端用 6 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNF a protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Please avoid freeze thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
请避免冻融循环。

Purity

Greater than 90.0% as determined by-PAGE.

Biological Activity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

The ED50 for this effect is ≤ 0.2 ng/ml.

在代谢抑制剂放线菌素 D 存在下，使用 L929 小鼠纤维肉瘤细胞在细胞毒性试验中测量。

这种效应的 ED50 ≤ 0.2 ng/ml。

Amino acid sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • TNF alpha human
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ProSpec-TNF a Mouse/CYT-252,Tumor Necrosis Factor-Alpha Mouse Recombinant

ProSpec-TNF a Mouse/CYT-252,Tumor Necrosis Factor-Alpha Mouse Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor Necrosis Factor-a Mouse Recombinant 在大肠杆菌中产生是一种单一的、非糖基化的多肽链，含有 157 个氨基酸，分子量为 17301.32 道尔顿。

TNF a Mouse
Tumor Necrosis Factor-Alpha Mouse Recombinant
CYT-252
5µg
20µg
1mg

Catalogue number

CYT-252

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

Tumor Necrosis Factor-a Mouse Recombinant produced in E. coli is a single, non glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17301.32 Dalton.
The TNF-alpha is purified by standard chromatographic techniques.
Tumor Necrosis Factor-a Mouse Recombinant 在大肠杆菌中产生是一种单一的、非糖基化的多肽链，含有 157 个氨基酸，分子量为 17301.32 道尔顿。
TNF-α通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.2.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的肿瘤坏死因子-α，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子-a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后的 TNF-a 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Biological Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.1ng/ml, corresponding to a Specific Activity of 10,000,000 Units/mg.

Protein content

Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.

References

Title:PUMA is directly activated by NF-?B and contributes to TNF-?-induced apoptosis.
Publication:Received 13 October 2008; Revised 3 April 2009; Accepted 3 April 2009; Published online 15 May 2009.
Link:http://www.nature.com/cdd/journal/v16/n9/full/cdd200951a.html

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF a Mutant Human/CYT-384,Tumor Necrosis Factor-Alpha Mutant Human Recombinant

ProSpec-TNF a Mutant Human/CYT-384,Tumor Necrosis Factor-Alpha Mutant Human Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor Necrosis Factor-a Variant Human Recombinant 产生于 E.Coli 是一种单一的、非糖基化的多肽链，含有 151 个氨基酸，分子量为 16598 道尔顿

TNF a Mutant Human
Tumor Necrosis Factor-Alpha Mutant Human Recombinant
CYT-384
10µg
50µg
1mg

Catalogue number

CYT-384

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

The clinical use of the potent anti-tumor activity of TNF-a has been limited by the proinflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been an intense pharmacological interest. Human TNF-a, which binds to the murine TNF-R55 but not to the mouse TNF-R75, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-a, which binds to both murine TNF receptors. Based on these results, many TNF-? mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo.
Recombinant Human TNF-a Variant/Mutant compared with the wild-type, has an amino acid sequence deletion from a.a. 1-7, and the following a.a. substitutes Arg8, Lys9, Arg10 and Phe157 which is proven tohave more activity and with less inflammatory side effect in vivo.
TNF-α 强效抗肿瘤活性的临床应用受到促炎副作用的限制，包括发热、剂量限制性低血压、肝毒性、血管内血栓形成和出血。设计具有低全身毒性的临床适用的 TNF-α 突变体一直是一个强烈的药理学兴趣。与小鼠 TNF-a 结合的小鼠 TNF-a 与小鼠 TNF-R55 结合但不与小鼠 TNF-R75 结合，在小鼠中表现出保留的抗肿瘤活性和降低的全身毒性，后者与两种小鼠 TNF 受体结合。基于这些结果，许多 TNF-?已经设计了选择性结合 TNF-R55 的突变体。这些突变体在体外对肿瘤细胞系表现出细胞毒活性，在体内表现出较低的全身毒性。
与野生型相比，重组人 TNF-a 变体/突变体具有来自 a.a. 的氨基酸序列缺失。 1-7，以及以下 a.a.替代 Arg8、Lys9、Arg10 和 Phe157，其在体内被证明具有更高的活性和更少的炎症副作用。

Description

Tumor Necrosis Factor-a Variant Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 151 amino acids and having a molecular mass of 16598 Dalton.
The TNF-alpha Variant is purified by standard chromatographic techniques.
Tumor Necrosis Factor-a Variant Human Recombinant 产生于 E.Coli 是一种单一的、非糖基化的多肽链，含有 151 个氨基酸，分子量为 16598 道尔顿。
TNF-α变体通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized after extensive dialysis against 0.5x PBS pH -7.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha Variant in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中重新配制冻干的肿瘤坏死因子-α 变异体，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Tumor Necrosis Factor-a Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a Variant should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子-a 变体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后 TNF-a 变体应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN
QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIAF.

Biological Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000 units/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF a Rabbit/CYT-008,Tumor Necrosis Factor-Alpha Rabbit Recombinant

ProSpec-TNF a Rabbit/CYT-008,Tumor Necrosis Factor-Alpha Rabbit Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。

TNF a Rabbit
Tumor Necrosis Factor-Alpha Rabbit Recombinant
CYT-008
5µg
20µg
1mg

Catalogue number

CYT-008

Synonyms

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E.Coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit.
The TNF-alpha is purified by standard chromatographic techniques.
Tumor Necrosis Factor-a Rabbit Recombinant 由三个相同的 158 个氨基酸的多肽链组成，结合形成紧凑的钟形同源三聚体。 TNF-α 在大肠杆菌中产生，是一种非糖基化的多肽链，单个亚基的分子量为 17.4 kDa。
TNF-α通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的肿瘤坏死因子-α，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子-a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后的 TNF-a 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg.

Biological Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30,000,000 IU/mg.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF a Rat/CYT-393,Tumor Necrosis Factor-Alpha Rat Recombinant

ProSpec-TNF a Rat/CYT-393,Tumor Necrosis Factor-Alpha Rat Recombinant,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在大肠杆菌中产生的肿瘤坏死因子-a 大鼠重组体是一个单一的、非糖基化的多肽链，含有 157 个氨基酸，分子量为 17339.44 道尔顿。 TNF-α通过标准色谱技术纯化。

TNF a Rat
Tumor Necrosis Factor-Alpha Rat Recombinant
CYT-393
5µg
20µg
1mg

Catalogue number

CYT-393

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, and cancer.

肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病——自身免疫疾病和癌症有关。

Description

Tumor Necrosis Factor-a Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17339.44 Dalton. The TNF-alpha is purified by standard chromatographic techniques.
在大肠杆菌中产生的肿瘤坏死因子-a 大鼠重组体是一个单一的、非糖基化的多肽链，含有 157 个氨基酸，分子量为 17339.44 道尔顿。 TNF-α通过标准色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The concentrated protein solution was lyophilized from 20mM phosphate buffer and 0.1M NaCl.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的肿瘤坏死因子-a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后的 TNF-a 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MLRSSSQNSS DKPVVHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL.

Biological Activity

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000 IU/mg.

通过在放线菌素 D 存在下对鼠 L929 细胞的细胞溶解测定的 ED50 为 < 0.05ng/ml，对应于 20,000,000 IU/mg 的比活性。

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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ProSpec-TNF a Rat, His/CYT-900,Tumor Necrosis Factor-alpha Rat Recombinant, His Tag

ProSpec-TNF a Rat, His/CYT-900,Tumor Necrosis Factor-alpha Rat Recombinant, His Tag,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Tumor Necrosis Factor-alpha, TNF a His, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.

TNF a Rat, His
Tumor Necrosis Factor-alpha Rat Recombinant, His Tag
CYT-900
5µg
20µg
1mg

Catalogue number

CYT-900

Synonyms

Tumor Necrosis Factor-alpha, TNF a His, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, N-terminal fragment, NTF, Intracellular domain 1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnfa, Tnfsf2, RATTNF, Tnfa.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
肿瘤坏死因子是一种参与全身炎症的细胞因子，是所有刺激急性期反应的一组细胞因子中的一员。 TNF主要由巨噬细胞分泌。
TNF导致凋亡细胞死亡、细胞增殖、分化、炎症、肿瘤发生和病毒复制，TNF还参与脂质代谢和凝血。 TNF的主要作用是调节免疫细胞。
失调，特别是 TNF 的过度产生与多种人类疾病有关——自身免疫疾病、胰岛素抵抗和癌症。

Description

TNF a Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids and having a molecular mass of 19.9kDa.
TNF a is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNF a Rat Recombinant 是一种单一的、非糖基化的多肽链，含有 181 个氨基酸，分子量为 19.9kDa。
TNF a 在 N 末端与 25 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

TNF a protein solution containing Phosphate Buffer Saline , 10% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by analysis by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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