标签： ProSpec产品

ProSpec-Clusterin/CYT-548

ProSpec Clusterin,ProSpec CYT-548,ProSpec Clusterin Rat,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Clusterin 也称为载脂蛋白 J，是一种 75-80 kD 二硫键连接的异二聚体蛋白，含有约 30% 的富含唾液酸的 N 连接碳水化合物，但也已鉴定出靶向细胞核的截短形式。

Clusterin

Human Clusterin
CYT-548
ProSpec-Clusterin/CYT-548 2µg
ProSpec-Clusterin/CYT-548 10µg
ProSpec-Clusterin/CYT-548 1mg

Catalogue number

CYT-548

Synonyms

CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, Apo-J.

Introduction

Clusterin also named Apolipoprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.
The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices.
Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen.
It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition.
A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates.
Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Clusterin 也称为载脂蛋白 J，是一种 75-80 kD 二硫键连接的异二聚体蛋白，含有约 30% 的富含唾液酸的 N 连接碳水化合物，但也已鉴定出靶向细胞核的截短形式。
前体多肽链被蛋白水解切割以去除 22-mer 分泌信号肽，随后在残基 227/228 之间生成 a 和 b 链。这些以反平行方式组装以产生异二聚体分子，其中富含半胱氨酸的中心通过五个二硫键连接，并且两侧是两个预测​​的卷曲螺旋α-螺旋和三个预测的两亲α-螺旋。
在广泛的物种中，clusterin 显示出高度的序列同源性，范围从 70% 到 80%。它几乎在大多数哺乳动物组织中普遍表达，可以在血浆、乳汁、尿液、脑脊液和精液中找到。
它能够与免疫球蛋白、脂质、肝素、细菌、补体成分、对氧磷酶、β淀粉样蛋白、瘦素等众多伙伴结合并形成复合物。凝聚素被赋予了过多的功能，例如吞噬细胞募集、聚集诱导、补体攻击预防、细胞凋亡抑制、膜重塑、脂质转运、激素转运和/或清除、基质金属蛋白酶抑制。
尚未定义 clusterin 的真正功能。一个诱人的假设说，凝聚素是一种细胞外伴侣蛋白，可保护细胞免受由降解和错误折叠的蛋白质沉淀物引起的应激损伤。
在包括癌症、器官再生、感染、阿尔茨海默病、色素性视网膜炎、心肌梗塞、肾小管损伤、自身免疫等在内的许多病理和临床相关情况下，Clusterin 的 mRNA 或蛋白质水平上调或下调。

Source

Plasma.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Human native Clusterin was filtered and lyophilized from 0.5mg/ml in 0.1M phosphate buffer, 0.15M NaCl pH 7.5.

Solubility

It is recommended to add deionized H2O to prepare a working stock solution of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入去离子水配制 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized Clusterin at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 Clusterin 储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95% as determined by PAGE.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • Clusterin Antibody
  • Clusterin Rat
  • Clusterin Human
  • Clusterin Canine
  • Clusterin Canine, HEK
  • Clusterin Human, Serum
  • Clusterin Mouse

ProSpec-Clusterin Canine, HEK/CYT-618

ProSpec Clusterin Canine, HEK,ProSpec CYT-618,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在 HEK293 细胞中产生的 Clusterin Canine Recombinant 是一种糖基化的多肽链，含有 436 个氨基酸，分子量为 50.72 kDa。 该蛋白在 N 末端融合了 13 个氨基酸的 Flag 标签。

Clusterin Canine, HEK

Clusterin Canine Recombinant, HEK
CYT-618
2µg
10µg
1mg

Catalogue number

CYT-618

Synonyms

CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Glycoprotein 80, Gp80, CLU.

Introduction

Clusterin mRNA and Clusterin protein are shown to increase with androgen treatment. Binding of clusterin to the LDL-Receptor plays a role in the pathogenesis of membranous glomerulonephritis. Clusterin is down regulated in CaP in association with matched benign controls. Clusterin is involved in cellular senescence and tumorigenesis. Clusterin is involved in photo-oxidative cell death pathway. Clusterin is a functional tumor marker for the diagnosis of pediatric large cell lymphoma. Clusterin is activated in low pH. Clusterin is involved in the inhibition of NF-kappaB signaling through stabilization of IkappaBs thus results in suppression of tumor cell motility. N-terminal deletion of clusterin is vital for its alterations of biogenesis in esophageal squamous cell carcinoma.
Clusterin mRNA 和 Clusterin 蛋白显示随着雄激素治疗而增加。 凝聚素与 LDL 受体的结合在膜性肾小球肾炎的发病机制中起作用。 与匹配的良性对照相关，Clusterin 在 CapP 中被下调。 凝聚素参与细胞衰老和肿瘤发生。 凝聚素参与光氧化细胞死亡途径。 Clusterin 是诊断小儿大细胞淋巴瘤的功能性肿瘤标志物。 凝聚素在低 pH 值下被激活。 Clusterin 通过稳定 IkappaB 参与抑制 NF-kappaB 信号传导，因此导致抑制肿瘤细胞运动。 簇蛋白的 N 末端缺失对其在食管鳞状细胞癌中的生物发生改变至关重要。

Description

Clusterin Canine Recombinant produced in HEK293 cells is a glycosylated, Polypeptide chain containing 436 amino acids and having a molecular mass of 50.72 kDa. The protein is fused with 13 amino acid Flag tag at N-Terminus.
The Apolipoprotein-J Canine is purified by proprietary chromatographic techniques.
在 HEK293 细胞中产生的 Clusterin Canine Recombinant 是一种糖基化的多肽链，含有 436 个氨基酸，分子量为 50.72 kDa。 该蛋白在 N 末端融合了 13 个氨基酸的 Flag 标签。
载脂蛋白-J Canine 通过专有的色谱技术进行纯化。

Source

Human Embryonic Kidney 293 Cells.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Canine Clusterin was filtered and lyophilized from 0.5mg/ml solution containing 20mM Tris buffer and 20mM NaCl, pH 7.5.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95% as determined by PAGE.

Amino acid sequence

PGDYKDDDDK PAGDQAVSDT ELQEMSTEGS KYINKEIKNA LKGVKQIKTL IEQTNEERKS LLSNLEEAKK KKEDALNDTK DSETKLKASQ GVCNDTMMAL WEECKPCLKQ TCMKFYARVC RSGSGLVGHQ LEEFLNQSSP FYFWMNGDRI DSLLENDRQQ THALDVMQDS FNRASSIMDE LFQDRFFTRE PQDTYHYSPF SLFQRRPFFN PKFRIARNII PFPRFQPLNF HDMFQPFFDM IHQAQQAMDV NLHRIPYHFP IEFPEEDNRT VCKEIRHNST GCLKMKDQCE KCQEILSVDC SSNNPAQVQL RQELSNSLQI AEKFTKLYDE LLQSYQEKMF NTSSLLKQLN EQFSWVSQLA NLTQSEDPFY LQVTTVGSQT NVPVGFT KVVVKLFDSD PITVMIPEAV SRNNPKFMET VAEKALQEYRQKHREE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines

Related Products

  • Clusterin
  • Clusterin Rat
  • Clusterin Canine
  • Clusterin Human
  • Clusterin Mouse

ProSpec-Clusterin Human, His/CYT-814

ProSpec Clusterin Human His,ProSpec CYT-814,ProSpec Clusterin Mouse,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,CLI, AAG4, APOJ, KUB1, SGP2, SGP-2, SP-40, TRPM2, TRPM-2, MGC24903, Clusterin, ging-associated gene 4 protein, Apolipoprotein J,Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, CLU.

Clusterin Human, His

Apolipoprotein-J Human Recombinant, His Tag
CYT-814
5µg
20µg
1mg

Catalogue number

CYT-814

Synonyms

CLI, AAG4, APOJ, KUB1, SGP2, SGP-2, SP-40, TRPM2, TRPM-2, MGC24903, Clusterin, ging-associated gene 4 protein, Apolipoprotein J,Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2, Testosterone-repressed prostate message 2, CLU.
CLI、AAG4、APOJ、KUB1、SGP2、SGP-2、SP-40、TRPM2、TRPM-2、MGC24903、Clusterin、ging 相关基因 4 蛋白、载脂蛋白 J、补体细胞溶解抑制剂、补体相关蛋白 SP-40、 40，Ku70 结合蛋白 1，NA1/NA2，睾酮抑制的前列腺信息 2，CLU。

Introduction

Clusterin also named Apolipoprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.
The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices.
Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen.
It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition.
A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates.
Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Clusterin 也称为载脂蛋白 J，是一种 75-80 kD 二硫键连接的异二聚体蛋白，含有约 30% 的富含唾液酸的 N 连接碳水化合物，但也已鉴定出靶向细胞核的截短形式。
前体多肽链被蛋白水解切割以去除 22-mer 分泌信号肽，随后在残基 227/228 之间生成 a 和 b 链。这些以反平行方式组装以产生异二聚体分子，其中富含半胱氨酸的中心通过五个二硫键连接，并且两侧是两个预测​​的卷曲螺旋α-螺旋和三个预测的两亲α-螺旋。
在广泛的物种中，clusterin 显示出高度的序列同源性，范围从 70% 到 80%。它几乎在大多数哺乳动物组织中普遍表达，可以在血浆、乳汁、尿液、脑脊液和精液中找到。
它能够与免疫球蛋白、脂质、肝素、细菌、补体成分、对氧磷酶、β淀粉样蛋白、瘦素等众多伙伴结合并形成复合物。凝聚素被赋予了过多的功能，例如吞噬细胞募集、聚集诱导、补体攻击预防、细胞凋亡抑制、膜重塑、脂质转运、激素转运和/或清除、基质金属蛋白酶抑制。
尚未定义 clusterin 的真正功能。一个诱人的假设说，凝聚素是一种细胞外伴侣蛋白，可保护细胞免受由降解和错误折叠的蛋白质沉淀物引起的应激损伤。
在包括癌症、器官再生、感染、阿尔茨海默病、色素性视网膜炎、心肌梗塞、肾小管损伤、自身免疫等在内的许多病理和临床相关情况下，Clusterin 的 mRNA 或蛋白质水平上调或下调。

Description

Clusterin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 463 amino acids and having a molecular mass of 54.1kDa. Clusterin is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Clusterin Human Recombinant 是一种单一的、非糖基化的多肽链，含有 463 个氨基酸，分子量为 54.1kDa。 Clusterin 在 N 末端与 36 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

Clusterin protein solution contains 20mM Tris-HCl buffer , 0.15M NaCl, 10% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSDQTV SDNELQEMSN QGSKYVNKEI QNAVNGVKQI KTLIEKTNEE RKTLLSNLEE AKKKKEDALN ETRESETKLK ELPGVCNETM MALWEECKPC LKQTCMKFYA RVCRSGSGLV GRQLEEFLNQ SSPFYFWMNG DRIDSLLEND RQQTHMLDVM QDHFSRASSI IDELFQDRFF TREPQDTYHY LPFSLPHRRP HFFFPKSRIV RSLMPFSPYE PLNFHAMFQP FLEMIHEAQQ AMDIHFHSPA FQHPPTEFIR EGDDDRTVCR EIRHNSTGCL RMKDQCDKCR EILSVDCSTN NPSQAKLRRE LDESLQVAER LTRKYNELLK SYQWKMLNTS SLLEQLNEQF NWVSRLANLT QGEDQYYLRV TTVASHT DVPSGVTEVV VKLFDSDPIT VTVPVEVSRK NPKFMETVAE KALQEYRKKH REE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • Clusterin Mouse

ProSpec-BMP 5 Human/CYT-660,Bone Morphogenetic protein-5 Human Recombinant

ProSpec BMP 5 Human,ProSpec CYT-660,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ProSpec-BMP 5 Human/CYT-660,在大肠杆菌中产生的 BMP-5 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 139 个氨基酸，总分子量为 15.7 kDa。
BMP-5 通过专有的色谱技术进行纯化。

BMP 5 Human

Bone Morphogenetic protein-5 Human Recombinant
CYT-660
ProSpec-BMP 5 Human/CYT-660 5µg
ProSpec-BMP 5 Human/CYT-660 20µg
ProSpec-BMP 5 Human/CYT-660 1mg

Catalogue number

CYT-660

Synonyms

Bone morphogenetic protein 5, BMP-5, BMP5, MGC34244.

Introduction

BMP5 belongs to the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. This superfamily is comprised of large families of growth and differentiation factors. Bone morphogenetic proteins were initially identified by their ability of demineralizing bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.
BMP5 is an essential signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. It was shown that BMP-5 increases the levels of osteopontin, BMP-2, alkaline phosphatase and core binding factor alpha 1 mRNAs in human periodontal ligament cells. The BMP5 protein is expressed in normal synovial tissue and reduced in osteoarthritis and rheumatoid arthritis. BMP5 may have a role in certain cancers given that it is differentially regulated during the formation of different tumors.
BMP5 属于骨形态发生蛋白家族，它是转化生长因子-β 超家族的一部分。 这个超家族由生长和分化因子的大家族组成。 骨形态发生蛋白最初是通过它们使骨提取物脱矿质以在体内在骨骼外部位诱导软骨内成骨的能力来鉴定的。
BMP5 是小梁网和视神经乳头内必需的信号分子，可能在青光眼发病机制中发挥潜在作用。 结果表明，BMP-5 增加了人牙周膜细胞中骨桥蛋白、BMP-2、碱性磷酸酶和核心结合因子 α1 mRNA 的水平。 BMP5 蛋白在正常滑膜组织中表达，在骨关节炎和类风湿性关节炎中减少。 BMP5 可能在某些癌症中发挥作用，因为它在不同肿瘤的形成过程中受到不同的调节。

Description

BMP-5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 139 amino acids and having a total molecular mass of 15.7 kDa.
BMP-5 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 BMP-5 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 139 个氨基酸，总分子量为 15.7 kDa。
BMP-5 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The BMP-5 solution contains 10mM Sodium Citrate buffer and 10% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MAANKRKNQN RNKSSSHQDS SRMSSVGDYN TSEQKQACKK HELYVSFRDL GWQDWIIAPE GYAAFYCDGE CSFPLNAHMN ATNHAIVQTL VHLMFPDHVP KPCCAPTKLN AISVLYFDDS SNVILKKYRN MVVRSCGCH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-GDF5 Human/CYT-442,Growth Differentiation Factor-5 Human Recombinant

ProSpec GDF5 Human,ProSpec CYT-442,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。

GDF5 Human

Growth Differentiation Factor-5 Human Recombinant
CYT-442
ProSpec-GDF5 Human/CYT-442 10µg
ProSpec-GDF5 Human/CYT-442 50µg
ProSpec-GDF5 Human/CYT-442 1mg

Catalogue number

CYT-442

Synonyms

Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14.

Introduction

GDF-5 is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.

Description

Growth Differentiation Factor 5 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 120 amino acids and having a total molecular mass of 27.4kDa. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.
GDF5 is purified by proprietary chromatographic techniques.

介绍

GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。 该家族的成员是胚胎和成人组织中细胞生长和分化的调节剂。 该基因的突变与Hunter-Thompson型肢端发育不良有关； 短指，C型； 和软骨发育不良，Grebe型。 这些关联证实基因产物在骨骼发育中发挥作用。

描述

在大肠杆菌中产生的生长分化因子 5 人重组体是一种同型二聚体、非糖基化多肽链，含有 2 x 120 个氨基酸，总分子量为 27.4kDa。 为了实现细菌表达，将 Ala-Pro-Leu-Thr 的 N 端序列替换为 Lys。
GDF5 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized without any additives.

Solubility

It is recommended to reconstitute the lyophilized Growth Differentiation Factor-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的生长分化因子 5 用不低于 100µg/ml 的无菌 18MΩ-cm H2O 复溶，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Growth Differentiation Factor 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Differentiation Factor-5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干生长分化因子 5 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，生长分化因子 5 应在 4°C 下储存 2-7 天，并在 -18°C 以下供将来使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Biological Activity

GDF-5 activity as determined by the induction of alkaline phosphatase activity in ATDC5 cells is typically 10-20ng/ml.

Amino acid sequence

APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard solution of GDF5 as a Reference Standard.
蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.15 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. 反相高效液相色谱分析，使用 GDF5 的标准溶液作为参考标准。

References

Title:Growth/differentiation Factor-5 Induces Osteogenic Differentiation of Human Ligamentum Flavum Cells through Activation of ERK1/2 and p38 MAPK.
Publication:Cell Physiol Biochem 2010;26:179-186
Link:http://content.karger.com/produktedb/produkte.asp?doi=320526

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • GDF15 Human, His
  • GDF10 Human
  • GDF5 Human, His
  • GDF5 Mouse
  • GDF3 Human
  • GDF5 Mouse, His

ProSpec-GDF5 Human, His/CYT-653,Growth differentiation factor 5 Human Recombinant, His Tag

ProSpec GDF5 Human His,CYT-653,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14.

GDF5 Human, His

Growth differentiation factor 5 Human Recombinant, His Tag
CYT-653
ProSpec-GDF5 Human, His/CYT-653 5µg
ProSpec-GDF5 Human, His/CYT-653 25µg
ProSpec-GDF5 Human, His/CYT-653 1mg

Catalogue number

CYT-653

Synonyms

Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14.

Introduction

GDF-5 is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.
GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。 该家族的成员是胚胎和成人组织中细胞生长和分化的调节剂。 该基因的突变与Hunter-Thompson型肢端发育不良有关； 短指，C型； 和软骨发育不良，Grebe型。 这些关联证实基因产物在骨骼发育中发挥作用。

Description

GDF5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a total molecular mass of 15.8 kDa.
GDF5 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 GDF5 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 141 个氨基酸，总分子量为 15.8 kDa。
GDF5 在 N 末端与 20 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The GDF5 solution contains 10mM sodium citrate pH-3.5 and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • GDF5 Mouse
  • GDF5 Mouse, His
  • GDF5 Human

ProSpec-GDF5 Mouse/CYT-941,Growth and Differentiation factor 5 Mouse Recombinant

ProSpec GDF5 Mouse,ProSpec CYT-941,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。

GDF5 Mouse

Growth and Differentiation factor 5 Mouse Recombinant
CYT-941
ProSpec-GDF5 Mouse/CYT-941 10µg
ProSpec-GDF5 Mouse/CYT-941 50µg
ProSpec-GDF5 Mouse/CYT-941 1mg

Catalogue number

CYT-941

Synonyms

Bmp-14, Bp, GDF-5, Bone morphogenetic protein 14, GDF5, Growth/differentiation factor 5.

Introduction

GDF-5 is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.
GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。 该家族的成员是胚胎和成人组织中细胞生长和分化的调节剂。 该基因的突变与Hunter-Thompson型肢端发育不良有关； 短指，C型； 和软骨发育不良，Grebe型。 这些关联证实基因产物在骨骼发育中发挥作用。

Description

GDF5 Mouse Recombinant produced in E.coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 120 amino acids and having a molecular mass of 27.2kDa.
The GDF-5 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 GDF5 Mouse Recombinant 是一种非糖基化的二硫键连接的同源二聚体，包含 2 条 120 个氨基酸的链，分子量为 27.2kDa。
GDF-5 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

GDF-5 protein was lyophilized from a 0.2µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

Solubility

It is recommended to reconstitute the lyophilized GDF5 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 4mM HCl 中复溶冻干的 GDF5，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized GDF5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF-5 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 GDF5 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，GDF-5 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 96.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR.

Biological Activity

The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0µg/ml, corresponding to a specific activity of > 1000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • GDF5 Mouse, His
  • GDF5 Human, His
  • GDF5 Human

ProSpec-GDF5 Mouse, His/CYT-852,Growth differentiation factor 5 Mouse Recombinant, His Tag

ProSpec-GDF5 Mouse, His/CYT-852,Growth differentiation factor 5 Mouse Recombinant, His Tag,GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。

GDF5 Mouse, His

Growth differentiation factor 5 Mouse Recombinant, His Tag
CYT-852
5µg
20µg
1mg

Catalogue number

CYT-852

Synonyms

Bmp-14, Bp, GDF-5, Bone morphogenetic protein 14, GDF5.

Introduction

GDF-5 is a member of the bone morphogenetic protein family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.
GDF-5 是骨形态发生蛋白家族和 TGF-β 超家族的成员。 这组蛋白质的特征在于多元蛋白水解加工位点，该位点被切割以产生含有七个保守半胱氨酸残基的成熟蛋白。 该家族的成员是胚胎和成人组织中细胞生长和分化的调节剂。 该基因的突变与Hunter-Thompson型肢端发育不良有关； 短指，C型； 和软骨发育不良，Grebe型。 这些关联证实基因产物在骨骼发育中发挥作用。

Description

GDF5 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 143 amino acids and having a molecular mass of 16kDa.
GDF5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 GDF5 Mouse Recombinant 是一条单一的、非糖基化的多肽链，包含 143 个氨基酸，分子量为 16kDa。
GDF5 在 N 端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

GDF5 protein solution containing 20mM Tris-HCl buffer and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSAPLANRQ GKRPSKNLKA RCSRKALHVN FKDMGWDDWI IAPLEYEAFH CEGLCEFPLR SHLEPTNHAV IQTLMNSMDP ESTPPTCCVP TRLSPISILF IDSANNVVYK QYEDMVVESC GCR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • GDF5 Human
  • GDF5 Mouse
  • GDF5 Human, His

ProSpec-GDF7 Human/CYT-870

ProSpec GDF7 Human,ProSpec CYT-870,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,生长分化因子 7 属于 TGF-b 超家族蛋白的 BMP 家族。 GDF7 通过由 1 型和 II 型丝氨酸/苏氨酸激酶受体组成的异二聚体受体复合物引发其生物活性。 GDF7 信号传导导致 Smad 蛋白的磷酸化和活化。 GDF-7 也参与肌腱和韧带的形成和修复。 此外，GDF7 调节骨形成、间充质干细胞分化、神经元分化和轴突引导。

GDF7 Human

Growth and Differentiation factor 7 Human Recombinant
CYT-870
ProSpec-GDF7 Human/CYT-870 2µg
ProSpec-GDF7 Human/CYT-870 10µg
ProSpec-GDF7 Human/CYT-870 1mg

Catalogue number

CYT-870

Synonyms

Growth Differentiation Factor 7, GDF-7, Growth/Differentiation Factor 7, BMP12, GDF7.

Introduction

Growth Differentiation Factor-7 belongs to the BMP family of TGF-b superfamily proteins. GDF7 elicits its bioactivity via a heterodimeric receptor complex comprised of a type 1 and a type II serine/threonine kinase receptor. GDF7 signaling results in the phosphorylation and activation of Smad proteins. GDF-7 is also involved in tendon and ligament formation and repair. In addition, GDF7 regulates bone formation, mesenchymal stem cell differentiation, neuronal differentiation, and axon guidance.
生长分化因子 7 属于 TGF-b 超家族蛋白的 BMP 家族。 GDF7 通过由 1 型和 II 型丝氨酸/苏氨酸激酶受体组成的异二聚体受体复合物引发其生物活性。 GDF7 信号传导导致 Smad 蛋白的磷酸化和活化。 GDF-7 也参与肌腱和韧带的形成和修复。 此外，GDF7 调节骨形成、间充质干细胞分化、神经元分化和轴突引导。

Description

GDF7 Human Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 28kDa.
The GDF-7 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 GDF7 Human Recombinant 是一种二硫键连接的同型二聚体、非糖基化多肽链，包含 129 个氨基酸，分子量为 28kDa。
GDF-7 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered solution in HCl.

Solubility

It is recommended to reconstitute the lyophilized GDF-7 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 GDF-7，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized GDF7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF-7 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 GDF7 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，GDF-7 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR.

Biological Activity

The ED50, as determined by inducing alkaline phosphatase production by mouse ATDC5 cells, is less than 1.25µg/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • GDF7 Mouse

ProSpec-GDF7 Mouse/CYT-946,Growth and Differentiation factor 7 Mouse Recombinant

ProSpec GDF7 Mouse,ProSpec CYT-946,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,生长分化因子 7 属于 TGF-b 超家族蛋白的 BMP 家族。 GDF7 通过由 1 型和 II 型丝氨酸/苏氨酸激酶受体组成的异二聚体受体复合物引发其生物活性。

GDF7 Mouse

Growth and Differentiation factor 7 Mouse Recombinant
CYT-946
ProSpec-GDF7 Mouse/CYT-946,Growth and Differentiation factor 7 Mouse Recombinant 2µg
ProSpec-GDF7 Mouse/CYT-946,Growth and Differentiation factor 7 Mouse Recombinant 10µg
ProSpec-GDF7 Mouse/CYT-946,Growth and Differentiation factor 7 Mouse Recombinant 1mg

Catalogue number

CYT-946

Synonyms

Growth/differentiation factor 7, GDF-7, Gdf7.

Introduction

Growth Differentiation Factor-7 belongs to the BMP family of TGF-b superfamily proteins. GDF7 elicits its bioactivity via a heterodimeric receptor complex comprised of a type 1 and a type II serine/threonine kinase receptor. GDF7 signaling results in the phosphorylation and activation of Smad proteins. GDF-7 is also involved in tendon and ligament formation and repair. In addition, GDF7 regulates bone formation, mesenchymal stem cell differentiation, neuronal differentiation, and axon guidance.
生长分化因子 7 属于 TGF-b 超家族蛋白的 BMP 家族。 GDF7 通过由 1 型和 II 型丝氨酸/苏氨酸激酶受体组成的异二聚体受体复合物引发其生物活性。 GDF7 信号传导导致 Smad 蛋白的磷酸化和活化。 GDF-7 也参与肌腱和韧带的形成和修复。 此外，GDF7 调节骨形成、间充质干细胞分化、神经元分化和轴突引导。

Description

GDF7 Mouse Recombinant produced in E.coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 146 amino acids and having a molecular mass of 29.8kDa.
The GDF-7 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 GDF7 Mouse Recombinant 是一种非糖基化的二硫键连接的同源二聚体，包含 2 条 146 个氨基酸的链，分子量为 29.8kDa。
GDF-7 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

GDF7 protein was lyophilized from a 0.2µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

Solubility

It is recommended to reconstitute the lyophilized GDF-7 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized GDF7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF-7 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 GDF7 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，GDF-7 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR.

Biological Activity

The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5µg/ml, corresponding to a specific activity of > 2000 IU/mg.

通过诱导鼠 ATDC5 细胞产生碱性磷酸酶测定的 ED50 小于 0.5μg/ml，对应于 > 2000 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • GDF7 Human

ProSpec-TSG Human/CYT-873,Twisted Gastrulation Protein Human Recombinant

ProSpec TSG Human,ProSpec CYT-873,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,扭曲的原肠胚形成蛋白是一种分泌的富含半胱氨酸的蛋白质，它通过调节 BMP 信号传导在果蝇和非洲爪蟾的背侧/腹侧模式中发挥作用。 TSG 通过控制 BMP 拮抗剂 Chordin/Sog 的抑制作用和金属蛋白酶 xolloid/tolloid 的切割特性，充当 BMP 信号传导的激动剂。 TSG N 末端结构域直接结合 BMP 蛋白并显示 BMP 拮抗剂活性。

TSG Human

Twisted Gastrulation Protein Human Recombinant
CYT-873
ProSpec-TSG Human/CYT-873 10µg
ProSpec-TSG Human/CYT-873 50µg
ProSpec-TSG Human/CYT-873 1mg

Catalogue number

CYT-873

Synonyms

Twisted Gastrulation BMP Signaling Modulator 1, TSG, Twisted Gastrulation Homolog 1 , Twisted Gastrulation Protein Homolog 1, Twisted Gastrulation Homolog 1, TWSG1.

Introduction

Twisted gastrulation Protein is a secreted, cysteine-rich protein which has a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating BMP signaling. TSG functions as an agonist for BMP signaling by controlling the inhibitory actions of the BMP antagonist, Chordin/Sog, and the cleavage properties of the metalloprotease, xolloid/tolloid. TSG N-terminal domain binds BMP protein directly and displays BMP antagonist activity.
扭曲的原肠胚形成蛋白是一种分泌的富含半胱氨酸的蛋白质，它通过调节 BMP 信号传导在果蝇和非洲爪蟾的背侧/腹侧模式中发挥作用。 TSG 通过控制 BMP 拮抗剂 Chordin/Sog 的抑制作用和金属蛋白酶 xolloid/tolloid 的切割特性，充当 BMP 信号传导的激动剂。 TSG N 末端结构域直接结合 BMP 蛋白并显示 BMP 拮抗剂活性。

Description

TWSG1 Human Recombinant produced in CHO is a single, glycosylated, polypeptide chain containing 198 amino acids and having a molecular mass ranging from 35-43kDa on -PAGE due to glycosylation.
The TWSG1 is purified by proprietary chromatographic techniques.
在 CHO 中产生的 TWSG1 Human Recombinant 是单条糖基化多肽链，含有 198 个氨基酸，由于糖基化，-PAGE 上的分子量范围为 35-43kDa。
TWSG1 通过专有的色谱技术进行纯化。

Source

CHO.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered solution in PBS.

Solubility

It is recommended to reconstitute the lyophilized TSG in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TSG，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TSG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TSG should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 TSG 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，TSG 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

CNKALCASDV SKCLIQELCQ CRPGEGNCSC CKECMLCLGA LWDECCDCVG MCNPRNYSDT PPTSKSTVEE LHEPIPSLFR ALTEGDTQLN WNIVSFPVAE ELSHHENLVS FLETVNQPHH QNVSVPSNNV HAPYSSDKEH MCTVVYFDDC MSIHQCKISC ESMGASKYRW FHNACCECIG PECIDYGSKT VKCMN CMF.

Biological Activity

The ED50, as measured by its ability to inhibit alkaline phosphatase production induced by rHuBMP-6 in mouse ATDC5 cells, is less than 16µg/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-BST1 Human/CYT-1071,Bone Marrow Stromal Cell Antigen 1 Human Recombinant

ProSpec BST1 Human,ProSpec CYT-1071,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,BST1 , is a GPI anchored membrane protein which is part of the CD38 family. BST1 was initially recognized as a bone marrow stromal cell molecule. BST1 is an ectoenzyme sharing more than a few features with ADP-ribosyl cyclase CD38. BST1 together with CD38, exhibit both DP-ribosyl cyclase and cyclinc ADP ribose hydrolase activities.

BST1 Human

Bone Marrow Stromal Cell Antigen 1 Human Recombinant
CYT-1071
ProSpec-BST1 Human/CYT-1071 2µg
ProSpec-BST1 Human/CYT-1071 10µg
ProSpec-BST1 Human/CYT-1071 1mg

Catalogue number

CYT-1071

Synonyms

Bone Marrow Stromal Cell Antigen 1, ADP-Ribosyl Cyclase 2, Bone Marrow Stromal Antigen 1, Cyclic ADP-Ribose Hydrolase 2, NAD Nucleosidase, CADPr Hydrolase 2, ADP-Ribosyl Cyclase/Cyclic ADP-Ribose Hydrolase 2, CD157 Antigen, EC 3.2.2.6, CD157, BST-1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, Bone marrow stromal antigen 1, Cyclic ADP-ribose hydrolase 2, cADPr hydrolase 2.

骨髓基质细胞抗原 1、ADP-核糖基环化酶 2、骨髓基质抗原 1、环状 ADP-核糖水解酶 2、NAD 核苷酶、CADPr 水解酶 2、ADP-核糖基环化酶/环状 ADP-核糖水解酶 2、CD157 抗原、EC 3.2 .2.6、CD157、BST-1、ADP-核糖基环化酶/环状 ADP-核糖水解酶 2、ADP-核糖基环化酶 2、骨髓基质抗原 1、环状 ADP-核糖水解酶 2、cADPr 水解酶 2。

Introduction

BST1 , is a GPI anchored membrane protein which is part of the CD38 family. BST1 was initially recognized as a bone marrow stromal cell molecule. BST1 is an ectoenzyme sharing more than a few features with ADP-ribosyl cyclase CD38. BST1 together with CD38, exhibit both DP-ribosyl cyclase and cyclinc ADP ribose hydrolase activities. BST1 participates in rheumatoid arthritis due to its enhanced expression in RA-derived bone marrow stromal cell lines. Moreover, BST1 is expressed by cells of the myeloid lineage and could perform as a receptor with a signal transduction capability.

BST1 是一种 GPI 锚定膜蛋白，它是 CD38 家族的一部分。 BST1 最初被认为是一种骨髓基质细胞分子。 BST1 是一种与 ADP-核糖基环化酶 CD38 共享多个特征的外酶。 BST1 与 CD38 一起表现出 DP-核糖基环化酶和 cyclinc ADP 核糖水解酶活性。 BST1 参与类风湿性关节炎，因为它在 RA 衍生的骨髓基质细胞系中表达增强。 此外，BST1 由髓系细胞表达，可以作为具有信号转导能力的受体发挥作用。

Description

BST1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 267 amino acids and having a molecular mass of 30.5kDa. .
BST1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 BST1 是单条糖基化多肽链，包含 267 个氨基酸，分子量为 30.5kDa。 .
BST1 在 C 末端用 6 个氨基酸的 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

BST1 protein solution contains 10% glycerol & Phosphate Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

RWRGEGTSAH LRDIFLGRCA EYRALLSPEQ RNKNCTAIWE AFKVALDKDP CSVLPSDYDL FINLSRHSIP RDKSLFWENS HLLVNSFADN TRRFMPLSDV LYGRVADFLS WCRQKNDSGL DYQSCPTSED CENNPVDSFW KRASIQYSKD SSGVIHVMLN GSEPTGAYPI KGFFADYEIP NLQKEKITRI EIWVMHEIGG PNVESCGEGS MKVLEKRLKD MGFQYSCIND YRPVKLLQCV DHSTHPDCAL KSAAAATQRK AHHHHHH.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-BST2 Human/CYT-059

ProSpec BST2 Human,ProSpec CYT-059,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,BST2 参与 B 细胞的生长和发育。 人类细胞蛋白 BST2 通过在受感染细胞出芽后维持病毒颗粒的扩散来抑制逆转录病毒感染。 BST2 最初被发现是在没有 Vpu 的情况下作为 HIV-1 感染的抑制剂，但也已知它可以抑制其他病毒的释放，例如 Lassa 和 Marburg 病毒粒子。 此外，BST2 在类风湿性关节炎中参与 B 细胞活化。

BST2 Human

Bone Marrow Stromal Cell Antigen 2 Human Recombinant
CYT-059
ProSpec-BST2 Human/CYT-059 2µg
ProSpec-BST2 Human/CYT-059 10µg
ProSpec-BST2 Human/CYT-059 1mg

Catalogue number

CYT-059

Synonyms

Bone marrow stromal cell antigen 2, CD317 antigen, BST-2, HM1.24 antigen, Tetherin,
NPC-A-7.

Introduction

BST2 takes part in the growth and development of B-cells. The human cellular protein BST2 inhibits retrovirus infection by maintaining the diffusion of virus particles after budding from infected cells. BST2 was originally discovered as an inhibitor to HIV-1 infection in the absence of Vpu, but it is also known to inhibit the release of other viruses such as the Lassa and Marburg virions. In addition, BST2 has a part in B-cell activation in rheumatoid arthritis.
BST2 参与 B 细胞的生长和发育。 人类细胞蛋白 BST2 通过在受感染细胞出芽后维持病毒颗粒的扩散来抑制逆转录病毒感染。 BST2 最初被发现是在没有 Vpu 的情况下作为 HIV-1 感染的抑制剂，但也已知它可以抑制其他病毒的释放，例如 Lassa 和 Marburg 病毒粒子。 此外，BST2 在类风湿性关节炎中参与 B 细胞活化。

Description

BST2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 14.8 kDa.
The BST2 is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 BST2 Human Recombinant 是单条非糖基化多肽链，包含 133 个氨基酸，分子量为 14.8 kDa。
BST2 在 N 末端与 21 个氨基酸的 His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

BST2 is supplied as a sterile filtered clear solution.

Formulation

BST2 protein 0.5mg/ml is supplied in 20mM Tris-HCl, pH-8, 0.1M NaCl, 1mM DTT and 20% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 80.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MSEACRDGLR AVMECRNVTH LLQQELTEAQ KGFQDVEAQA ATCNHTVMAL MASLDAEKAQ GQKKVEELEG EITTLNHKLQ DASAEVERLR RENQVLSVRI ADKKYYPSSQ DSS

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-BNP/CYT-327,B-type Natriuretic Peptide Human Recombinant

ProSpec BNP,ProSpec CYT-327,B-type Natriuretic Peptide Human Recombinant,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,冻干的 B 型利钠肽虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，NPPB 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

BNP

B-type Natriuretic Peptide Human Recombinant
CYT-327
ProSpec-BNP/CYT-327 2µg
ProSpec-BNP/CYT-327 10µg
ProSpec-BNP/CYT-327 1mg

Catalogue number

CYT-327

Synonyms

NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.

Introduction

Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body’s salt and water balance. Improves heart function.
利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利尿钠、利尿、血管舒张以及抑制肾素和醛固酮分泌。 它被认为在心血管稳态中起关键作用。 帮助恢复身体的盐分和水分平衡。 改善心脏功能。

Description

B-type Natriuretic Peptide Recombinant Human produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 32 amino acids and having a molecular mass of 3,500 Dalton. NPPB is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 B 型利钠肽重组人是单条非糖基化多肽链，含有 32 个氨基酸，分子量为 3,500 道尔顿。 NPPB 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Natriuretic Peptide Precursor B was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized B-type Natriuretic Peptide in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized B-type Natriuretic Peptide although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NPPB should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 B 型利钠肽虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，NPPB 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

SPKMVQGSGCFGRKMDRISSSSGLGCKVLRRH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • BNP Human, His
  • BNP Human
  • BNP Human
  • NT-proBNP Human
  • proBNP Human

ProSpec-BNP (27-102) Human/CYT-1029

ProSpec BNP (27-102) Human,ProSpec CYT-1029,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,BNP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids including a 7 a.a N-terminal His tag. The total molecular mass is 9.4kDa . 

BNP Human

B-type Natriuretic Protein Human Recombinant
CYT-1029
ProSpec-BNP (27-102) Human/CYT-1029 2µg
ProSpec-BNP (27-102) Human/CYT-1029 10µg
ProSpec-BNP (27-102) Human/CYT-1029 1mg

Catalogue number

CYT-1029

Synonyms

NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.

Introduction

Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body’s salt and water balance. Improves heart function.

利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利尿钠、利尿、血管舒张以及抑制肾素和醛固酮分泌。 它被认为在心血管稳态中起关键作用。 帮助恢复身体的盐分和水分平衡。 改善心脏功能。

Description

BNP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids including a 7 a.a N-terminal His tag. The total molecular mass is 9.4kDa .

在大肠杆菌中产生的 BNP Human Recombinant 是一条单一的、非糖基化的多肽链，包含 83 个氨基酸，包括一个 7 个氨基酸的 N 端 His 标签。 总分子量为9.4kDa。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

BNP filtered and lyophilized from 0.5mg/ml in 20 mM Tris buffer and 50 mM NaCl, pH 7.5.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. BNP is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 BNP 不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHPL GSPGSASDLE TSGLQEQRNH LQGKLSELQV EQTSLEPLQE SPRPTGVWKS REVATEGIRG HRKMVLYTLR APR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • BNP
  • BNP Human
  • BNP Human, His
  • NT-proBNP Human
  • proBNP Human

ProSpec-BNP Human/CYT-369,B-type Natriuretic Peptide Human

ProSpec BNP Human,ProSpec CYT-369,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利尿钠、利尿、血管舒张以及抑制肾素和醛固酮分泌。 它被认为在心血管稳态中起关键作用。 帮助恢复身体的盐分和水分平衡。 改善心脏功能。

BNP Human

B-type Natriuretic Peptide Human
CYT-369
10mg
25mg
100mg

Catalogue number

CYT-369

Synonyms

NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.

Introduction

Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body’s salt and water balance. Improves heart function.
利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利尿钠、利尿、血管舒张以及抑制肾素和醛固酮分泌。 它被认为在心血管稳态中起关键作用。 帮助恢复身体的盐分和水分平衡。 改善心脏功能。

Description

B-type Natriuretic Peptide Human is a polypeptide chain containing 32 amino acids and having a molecular mass of 3464 Dalton. The molecular formula is:C143H244N50O42S4.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized without additives.

Solubility

It is recommended to reconstitute the lyophilized B-type Natriuretic Peptide in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 B 型利钠肽在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized B-type Natriuretic Peptide although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution B-type Natriuretic Peptide should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 B 型利钠肽虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，B 型利钠肽应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by RP-HPLC.

Amino acid sequence

SPKMVQGSGCFGRKMDRISSSSGLGCKVLRRH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • BNP
  • BNP Human, His
  • BNP Human
  • NT-proBNP Human
  • proBNP Human

ProSpec-BNP Human, His/CYT-605

ProSpec BNP Human His,ProSpec CYT-605,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.

BNP Human, His

B-type Natriuretic Peptide Human Recombinant, His Tag
CYT-605
ProSpec-BNP Human, His/CYT-605 2µg
ProSpec-BNP Human, His/CYT-605 10µg
ProSpec-BNP Human, His/CYT-605 1mg

Catalogue number

CYT-605

Synonyms

NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.

Introduction

Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body’s salt and water balance. Improves heart function.
利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利尿钠、利尿、血管舒张以及抑制肾素和醛固酮分泌。 它被认为在心血管稳态中起关键作用。 帮助恢复身体的盐分和水分平衡。 改善心脏功能。

Description

BNP Recombinant Human fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 14 kDa.
BNP is purified by proprietary chromatographic techniques.
BNP Recombinant Human 在 N 末端融合了 20 个氨基酸的 His 标签，在大肠杆菌中产生，是一条单一的、非糖基化的多肽链，包含 129 个氨基酸，分子量为 14 kDa。
BNP 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

BNP 1mg/ml is formulated with 1X PBS pH-7.4, 2mM DTT, 0.1mM PMSF, 1mM EDTA and 40% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 90.0% as determined by analysis by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLSELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • BNP
  • BNP Human
  • BNP Human
  • NT-proBNP Human
  • proBNP Human

ProSpec-NPPA Human/CYT-1028,Natriuretic Peptide A Human Recombinant

ProSpec NPPA Human,ProSpec CYT-1028,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Natriuretic peptides A, CDD-ANF, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, N-terminal proatrial natriuretic peptide, ANP, PND. 

NPPA Human

Natriuretic Peptide A Human Recombinant
CYT-1028
ProSpec-NPPA Human/CYT-1028 2µg
ProSpec-NPPA Human/CYT-1028 10µg
ProSpec-NPPA Human/CYT-1028 1mg

Catalogue number

CYT-1028

Synonyms

Natriuretic peptides A, CDD-ANF, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, N-terminal proatrial natriuretic peptide, ANP, PND.

Introduction

Natriuretic Peptide A is a part of the natriuretic peptide family and is involved  in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. NPPA is synthesized as a great precursor which releases a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. In female pregnancy, NPPA is promoting trophoblast invasion and spiral artery remodeling in uterus.

利钠肽 A 是利钠肽家族的一部分，通过调节利钠、利尿和血管舒张参与心血管稳态。 NPPA 被合成为一种伟大的前体，它从 N 端释放一种与血管活性肽、心脏扩张肽相似的肽，并从 C 端释放另一种具有利钠利尿活性的肽。 在女性妊娠期，NPPA 促进子宫内滋养层侵袭和螺旋动脉重塑。

Description

NPPA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 106 amino acids including an 8 a.a N-terminal His tag. The total molecular mass is 11.7kDa .

在大肠杆菌中产生的 NPPA Human Recombinant 是一种单一的、非糖基化的多肽链，包含 106 个氨基酸，包括一个 8 个氨基酸的 N 端 His 标签。 总分子量为11.7kDa。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

NPPA filtered and lyophilized from 0.5mg/ml in 20 mM Tris buffer, 50 mM NaCl and 5% w/v trehalosa, pH 7.5.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. NPPA is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 NPPA 不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHNP MYNAVSNADL MDFKNLLDHL EEKMPLEDEV VPPQVLSEPN EEAGAALSPL PEVPPWTGEV SPAQRDGGAL GRGPWDSSDR SALLKSKLRA LLTAPR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-NPPC Human/CYT-760,Natriuretic Peptide C Human Recombinant

ProSpec NPPC Human,ProSpec CYT-760,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,NPPC 被蛋白水解处理以产生利钠肽家族的分泌激素。 NPPC 具有血管活性和利钠作用，并控制软骨生长板软骨细胞的进化和分化。

NPPC Human

Natriuretic Peptide C Human Recombinant
CYT-760
ProSpec-NPPC Human/CYT-760 5µg
ProSpec-NPPC Human/CYT-760 20µg
ProSpec-NPPC Human/CYT-760 1mg

Catalogue number

CYT-760

Synonyms

Natriuretic Peptide Precursor C, C-Type Natriuretic Peptide, CNP2, CNP.

Introduction

NPPC is proteolytically managed to create a secreted hormone of the natriuretic peptide family. NPPC is vasoactive and natriuretic and controls the evolution and differentiation of cartilaginous growth plate chondrocytes.
NPPC 被蛋白水解处理以产生利钠肽家族的分泌激素。 NPPC 具有血管活性和利钠作用，并控制软骨生长板软骨细胞的进化和分化。

Description

NPPC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 13.2kDa.
NPPC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 NPPC Human Recombinant 是单条非糖基化多肽链，包含 126 个氨基酸，分子量为 13.2kDa。
NPPC 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The NPPC solution contains 20mM Tris-HCl buffer , 0.15M NaCl and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSKPGAPPK VPRTPPAEEL AEPQAAGGGQ KKGDKAPGGG GANLKGDRSR LLRDLRVDTK SRAAWARLLQ EHPNARKYKG ANKKGLSKGC FGLKLDRIGS MSGLGC

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpecs 产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-NT-proBNP Human/CYT-1118

ProSpec NT-proBNP Human,ProSpec CYT-1118,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利钠、血管舒张、利尿以及抑制肾素和醛固酮的分泌。 BNP 在心血管稳态中起关键作用。 BNP 还有助于恢复身体的盐和水平衡并改善心脏功能。

NT-proBNP Human

NT-Pro-B-type Natriuretic Protein Human Recombinant
CYT-1118
ProSpec-NT-proBNP Human/CYT-1118 10µg
ProSpec-NT-proBNP Human/CYT-1118 50µg
ProSpec-NT-proBNP Human/CYT-1118 1mg

Catalogue number

CYT-1118

Synonyms

NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide.

Introduction

Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis,vasorelaxation,diuresis and inhibition of renin and aldosterone secretion. BNP plays a key role in cardiovascular homeostasis. BNP also helps restore the body’s salt and water balance andimproves heart function.

利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利钠、血管舒张、利尿以及抑制肾素和醛固酮的分泌。 BNP 在心血管稳态中起关键作用。 BNP 还有助于恢复身体的盐和水平衡并改善心脏功能。

Description

NT-Pro-B-type Natriuretic Protein Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 76 amino acid and having a molecular mass of approximately 8.5kDa.

NT-proBNP is purified by proprietary chromatographic techniques.

NT-Pro-B-type Natriuretic Protein Human Recombinant 在大肠杆菌中产生，是一种非糖基化多肽链，含有 76 个氨基酸，分子量约为 8.5kDa。

NT-proBNP 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0 and 150mM NaCl.

从 20mM Tris-HCl、pH 8.0 和 150mM NaCl 中的 0.2μm 过滤浓缩溶液冻干。

Solubility

It is recommended to reconstitute the lyophilized NT-Pro-B-type Natriuretic Protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 NT-Pro-B 型利钠蛋白在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized NT-proBNP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NT-Pro-B-type Natriuretic Protein should be stored at 4°C between 2-7 days and for future use below -18°C.

冻干的 NT-proBNP 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，NT-Pro-B 型利尿钠蛋白应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

HPLGSPGSAS DLETSGLQEQ RNHLQGKLSE LQVEQTSLEP LQESPRPTGV WKSREVATEG IRGHRKMVLY TLRAPR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • BNP Human
  • BNP
  • BNP Human
  • BNP Human, His
  • proBNP Human

ProSpec-proBNP Human/CYT-1205

ProSpec proBNP Human,ProSpec CYT-1205,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在大肠杆菌中产生的重组人 Pro B 型利尿钠蛋白是单一的、非糖基化的多肽链，与 His 标签融合，分子量约为 13kDa。proBNP 通过专有的色谱技术进行纯化。

proBNP Human

Pro B-type Natriuretic Protein Human Recombinant
CYT-1205
ProSpec-proBNP Human/CYT-1205 25µg
ProSpec-proBNP Human/CYT-1205 50µg
ProSpec-proBNP Human/CYT-1205 100µg

Catalogue number

CYT-1205

Synonyms

NPPB, Natriuretic Peptide Precursor B, BNP, B-type Natriuretic Peptide

Introduction

Natriuretic Peptide Precursor B acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body’s salt and water balance. Improves heart function.

利钠肽前体 B 作为一种心脏激素，具有多种生物作用，包括利尿钠、利尿、血管舒张以及抑制肾素和醛固酮分泌。 它被认为在心血管稳态中起关键作用。 帮助恢复身体的盐分和水分平衡。 改善心脏功能。

Description

Recombinant Human Pro B-type Natriuretic Protein produced in E.Coli is a single, non-glycosylated, polypeptide chain , fused to His-tag and having a molecular weight of ~13kDa.
The proBNP is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的重组人 Pro B 型利尿钠蛋白是单一的、非糖基化的多肽链，与 His 标签融合，分子量约为 13kDa。
proBNP 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid formulation.

Formulation

Human proBNP solution contains 500 mM NaCl, 20mM Tris-HCl, 4mM CaCl2, 4mM MgCl2and 60 mM B-mercaptoethanol, pH 7.5.

Stability

proBNP although stable at 10°C for 7 days, should be stored below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • BNP
  • BNP Human, His
  • BNP Human
  • BNP Human
  • NT-proBNP Human

ProSpec-CTF1 Human/CYT-944

ProSpec CTF1 Human,ProSpec CYT-944,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 IU/mg.

CTF1 Human

Cardiotrophin-1 Human Recombinant
CYT-944
ProSpec-CTF1 Human/CYT-944 2µg
ProSpec-CTF1 Human/CYT-944 10µg
ProSpec-CTF1 Human/CYT-944 1mg

Catalogue number

CYT-944

Synonyms

CTF1, CT1, CT-1, Cardiophin 1, Cardiotrophin-1.

Introduction

Cardiotrophin 1 is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.
CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins in cardiac cells.
Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide and angiotensinogen mRNA.
Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。 CT 1 在正常成人肺中也大量检测到，并在胎儿和成人气道平滑肌细胞中表达。 CT 1 激活 gp130 依赖性信号传导并刺激 Janus 激酶/信号转导和转录途径激活因子，以转导心肌细胞中的肥大和细胞保护信号。
CT 1 还具有神经营养功能。在胚胎第 14 天和出生后第一周之间，CTF1 缺乏导致小鼠脊髓和脑干核中运动神经元细胞死亡增加。此外，CT-1 是一种肝细胞存活因子，可有效减少急性肝损伤动物模型中的肝细胞损伤。心肌营养因子 1 的表达在缺氧刺激后增强，当在模拟缺血之前或在模拟缺血后的复氧时添加时，它可以保护心肌细胞。 Cardiotrophin 1 可以诱导心脏细胞中保护性热休克蛋白的表达。
Cardiotrophin-1 增加 ANP、脑利钠肽和血管紧张素原 mRNA 的心室表达。
Cardiophin 1 水平在心力衰竭患者、扩张型心肌病患者、中/重度二尖瓣关闭不全、稳定和不稳定型心绞痛患者以及急性心肌梗死后显着升高。

Description

Cardiotrophin-1 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 201 amino acids and having a molecular mass of 21.2kDa.
The CTF1 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Cardiotrophin-1 Human Recombinant 是一条单一的、非糖基化的多肽链，含有 201 个氨基酸，分子量为 21.2kDa。
CTF1 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

CTF-1 protein was lyophilized from a 0.2µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

Solubility

It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 4mM HCl 中复溶冻干的 CTF1，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized CTF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF-1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 CTF1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CTF-1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A.

Biological Activity

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • CTF1 Human, His
  • CTF1 Rat
  • CTF1 Mouse

ProSpec-CTF1 Human, His/CYT-436

ProSpec CTF1 Human, His,ProSpec CYT-436,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Purity of CTF1 Human Recombinant is greater than 90% as determined by -PAGE.

CTF1 Human, His

Cardiotrophin-1 Human Recombinant, His Tag
CYT-436
ProSpec-CTF1 Human, His/CYT-436 2µg
ProSpec-CTF1 Human, His/CYT-436 10µg
ProSpec-CTF1 Human, His/CYT-436 1mg

Catalogue number

CYT-436

Synonyms

CTF1, CT1, CT-1, Cardiophin 1.

Introduction

Cardiotrophin 1 is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.
CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins in cardiac cells.
Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide and angiotensinogen mRNA.
Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。 CT 1 在正常成人肺中也大量检测到，并在胎儿和成人气道平滑肌细胞中表达。 CT 1 激活 gp130 依赖性信号传导并刺激 Janus 激酶/信号转导和转录途径激活因子，以转导心肌细胞中的肥大和细胞保护信号。
CT 1 还具有神经营养功能。在胚胎第 14 天和出生后第一周之间，CTF1 缺乏导致小鼠脊髓和脑干核中运动神经元细胞死亡增加。此外，CT-1 是一种肝细胞存活因子，可有效减少急性肝损伤动物模型中的肝细胞损伤。心肌营养因子 1 的表达在缺氧刺激后增强，当在模拟缺血之前或在模拟缺血后的复氧时添加时，它可以保护心肌细胞。 Cardiotrophin 1 可以诱导心脏细胞中保护性热休克蛋白的表达。
Cardiotrophin-1 增加 ANP、脑利钠肽和血管紧张素原 mRNA 的心室表达。
Cardiophin 1 水平在心力衰竭患者、扩张型心肌病患者、中/重度二尖瓣关闭不全、稳定和不稳定型心绞痛患者以及急性心肌梗死后显着升高。

Description

The Cardiotrophin His-Tagged Fusion Protein Human, produced in E. coli, is 22.5 kDa protein containing 200 amino acid residues of the human Cardiotrophin and 12 additional amino acid residues – His Tag .
在大肠杆菌中产生的 Cardiotrophin His-Tagged Fusion Protein Human 是一种 22.5 kDa 的蛋白质，含有人 Cardiotrophin 的 200 个氨基酸残基和 12 个额外的氨基酸残基 – His 标签。

Source

Escherichia Coli.

Formulation

CTF1 was filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Solubility

It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10?g/ml. In higher concentrations the solubility of this antigen is limited. Protein is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入 0.1M 醋酸盐缓冲液 pH-4 制备约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10?g/ml 的浓度。 在较高浓度下，该抗原的溶解度是有限的。 蛋白质不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MRGSHHHHHH GSSRREGSLE DPQTDSSVSL LPHLEAKIRQ THSLAHLLTK YAEQLLQEYV QLQGDPFGLPSFSPPRLPVA GLSAPAPSHA GLPVHERLRL DAAALAALPP LLDAVCRRQA ELNPRAPRLL RRLEDAARQA RALGAAVEAL LAALGAANRG PRAEPPAATA SAASATGVFP AKVLGLRVCG LYREWLSRTE GDLGQLLPGG SA.

Purity

Purity of CTF1 Human Recombinant is greater than 90% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • CTF1 Mouse
  • CTF1 Human
  • CTF1 Rat

ProSpec-CTF1 Mouse/CYT-151

ProSpec CTF1 Mouse,ProSpec CYT-151,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,由 TF-1 细胞的剂量依赖性增殖确定的 ED50 < 1.0ng/ml，对应于 > 1,000,000units/mg 的比活性。

CTF1 Mouse

Cardiotrophin-1 Mouse Recombinant
CYT-151
ProSpec-CTF1 Mouse/CYT-151 2µg
ProSpec-CTF1 Mouse/CYT-151 10µg
ProSpec-CTF1 Mouse/CYT-151 1mg

Catalogue number

CYT-151

Synonyms

CTF1, CT1, CT-1, Cardiophin 1.

Introduction

Cardiotrophin 1 is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.
CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins in cardiac cells.
Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide and angiotensinogen mRNA.
Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。 CT 1 在正常成人肺中也大量检测到，并在胎儿和成人气道平滑肌细胞中表达。 CT 1 激活 gp130 依赖性信号传导并刺激 Janus 激酶/信号转导和转录途径激活因子，以转导心肌细胞中的肥大和细胞保护信号。
CT 1 还具有神经营养功能。在胚胎第 14 天和出生后第一周之间，CTF1 缺乏导致小鼠脊髓和脑干核中运动神经元细胞死亡增加。此外，CT-1 是一种肝细胞存活因子，可有效减少急性肝损伤动物模型中的肝细胞损伤。心肌营养因子 1 的表达在缺氧刺激后增强，当在模拟缺血之前或在模拟缺血后的复氧时添加时，它可以保护心肌细胞。 Cardiotrophin 1 可以诱导心脏细胞中保护性热休克蛋白的表达。
Cardiotrophin-1 增加 ANP、脑利钠肽和血管紧张素原 mRNA 的心室表达。
Cardiophin 1 水平在心力衰竭患者、扩张型心肌病患者、中/重度二尖瓣关闭不全、稳定和不稳定型心绞痛患者以及急性心肌梗死后显着升高。

Description

CTF1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 202 amino acids and having a molecular mass of 21.3kDa.
The CTF1 Mouse is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 CTF1 小鼠重组体是一条单一的、非糖基化的多肽链，含有 202 个氨基酸，分子量为 21.3kDa。
CTF1 Mouse 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl to a concentration of 0.1-0.5 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-200C. Further dilutions should be made in appropriate buffered solutions.

Stability

Lyophilized CTF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
冻干的 CTF1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CTF1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Purity

Greater than 98.0% as determined by -PAGE.

Amino acid sequence

SQREGSLEDH QTDSSISFLP HLEAKIRQTH NLARLLTKYA EQLLEEYVQQ QGEPFGLPGF SPPRLPLAGL SGPAPSHAGL PVSERLRQDA AALSVLPALL DAVRRRQAEL NPRAPRLLRS LEDAARQVRA LGAAVETVLA ALGAAARGPG PEPVTVATLF TANSTAGIFS AKVLGFHVCG LYGEWVSRTE GDLGQLVPGG VA

Biological Activity

The ED50 as determined by the dose-dependent proliferation of TF-1 cells was < 1.0ng/ml, corresponding to a specific activity of > 1,000,000units/mg.
由 TF-1 细胞的剂量依赖性增殖确定的 ED50 < 1.0ng/ml，对应于 > 1,000,000units/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • CTF1 Human, His
  • CTF1 Human
  • CTF1 Rat

ProSpec-CTF1 Rat/CYT-199,Cardiotrophin-1 Rat Recombinant

ProSpec-CTF1 Rat/CYT-199,Cardiotrophin-1 Rat Recombinant,心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。

CTF1 Rat

Cardiotrophin-1 Rat Recombinant
CYT-199
ProSpec-CTF1 Rat/CYT-199 2µg
ProSpec-CTF1 Rat/CYT-199 10µg
ProSpec-CTF1 Rat/CYT-199 1mg

Catalogue number

CYT-199

Synonyms

Cardiotrophin-1, CT-1, Ctf1.

Introduction

Cardiotrophin 1 is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.
CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins in cardiac cells.
Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide and angiotensinogen mRNA.
Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。 CT 1 在正常成人肺中也大量检测到，并在胎儿和成人气道平滑肌细胞中表达。 CT 1 激活 gp130 依赖性信号传导并刺激 Janus 激酶/信号转导和转录途径激活因子，以转导心肌细胞中的肥大和细胞保护信号。
CT 1 还具有神经营养功能。在胚胎第 14 天和出生后第一周之间，CTF1 缺乏导致小鼠脊髓和脑干核中运动神经元细胞死亡增加。此外，CT-1 是一种肝细胞存活因子，可有效减少急性肝损伤动物模型中的肝细胞损伤。心肌营养因子 1 的表达在缺氧刺激后增强，当在模拟缺血之前或在模拟缺血后的复氧时添加时，它可以保护心肌细胞。 Cardiotrophin 1 可以诱导心脏细胞中保护性热休克蛋白的表达。
Cardiotrophin-1 增加 ANP、脑利钠肽和血管紧张素原 mRNA 的心室表达。
Cardiophin 1 水平在心力衰竭患者、扩张型心肌病患者、中/重度二尖瓣关闭不全、稳定和不稳定型心绞痛患者以及急性心肌梗死后显着升高。

Description

Cardiotrophin-1 Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 203 amino acids and having a molecular mass of 21.4kDa.
The CTF1 Rat is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Cardiotrophin-1 Rat Recombinant 是一种单一的、非糖基化的多肽链，含有 203 个氨基酸，分子量为 21.4kDa。
CTF1 大鼠通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 4mM HCl 中复溶冻干的 CTF1，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Cardiotrophin-1 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF1 Rat should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
冻干的 Cardiotrophin-1 大鼠虽然在室温下稳定 3 周，但应在 -18°C 以下干燥储存。 重组后，CTF1 大鼠应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE and HPLC analyses.

Amino acid sequence

MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA.

Biological Activity

The ED50 as determined by the dose-dependent proliferation of TF-1 cells was found to be < 0.5ng/ml, corresponding to a specific activity of > 2.0×106 units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • CTF1 Human
  • CTF1 Human, His
  • CTF1 Mouse

ProSpec-CTF2P Mouse/CYT-1128

ProSpec CTF2P Mouse,ProSpec CYT-1128,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,The ED50 as determined by a cell proliferation assay using human TF-1 cells is < 200 ng/ml, corresponding to a specific activity of > 5000 IU/mg.

CTF2P Mouse

Neuropoietin Mouse Recombinant
CYT-1128
ProSpec-CTF2P Mouse/CYT-1128 50µg
ProSpec-CTF2P Mouse/CYT-1128 100µg
ProSpec-CTF2P Mouse/CYT-1128 1mg

Catalogue number

CYT-1128

Synonyms

Cardiotrophin-2, CT-2, Neuropoietin, Np, Ctf2, Gm494.

Introduction

CTF2P, aka Neuropoietinis a part of the IL-6 family of cytokines. CTF2P is the outcome of a gene duplication event involving cardiotrophin-1 and it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. CTF2P Increases the platelet count associated with splenomegaly and takes part in neuronal precursor development and maturation.

CTF2P，又名 Neuropoietinis，是 IL-6 细胞因子家族的一部分。 CTF2P 是涉及心肌营养因子-1 的基因复制事件的结果，它有助于在 IL-6 家族中定义一个亚家族，包括 CT-1、CLC 和 CTNF。 CTF2P 增加与脾肿大相关的血小板计数并参与神经元前体发育和成熟。

Description

Neuropoietin Mouse Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 182 amino acids and having a molecular mass of approximately 19.7kDa.
CTF2P is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Neuropoietin Mouse Recombinant 是一种非糖基化多肽链，含有 182 个氨基酸，分子量约为 19.7kDa。
CTF2P 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 0.5mM DTT and 500mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Neuropoietin in sterile PBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的神经生成素在不低于 100μg/ml 的无菌 PBS 中复溶，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized CTF2P although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Neuropoietin should be stored at 4°C between 2-7 days and for future use below -18°C.

冻干的 CTF2P 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，Neuropoietin 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

APISPSEPIG QAYSLALYMQ KNTSALLQTY LQHQGSPFSD PGFSAPELQL STLPSAAVSF KTWHAMEDAE RLSRAQGAFL ALTQHLQLVG DDQSYLNPGS PILLAQLGAA RLRAQGLLGN MAAIMTALGL PIPPEEDTLG FVPFGASAFE RKCRGYIVTR EYGHWTDRAV RDLALLKAKY SA.

Biological Activity

The ED50 as determined by a cell proliferation assay using human TF-1 cells is < 200 ng/ml, corresponding to a specific activity of > 5000 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-CTLA 4 Human/CYT-366

ProSpec CTLA 4 Human,ProSpec CYT-366,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,The CTLA 4 solution contains 20mM Tris-HCl buffer , 0.4M urea and 10% glycerol.

CTLA 4 Human

Cytotoxic T-Lymphocyte Associated Antigen-4 Human Recombinant
CYT-366
ProSpec-CTLA 4 Human/CYT-366 5µg
ProSpec-CTLA 4 Human/CYT-366 25µg
ProSpec-CTLA 4 Human/CYT-366 1mg

Catalogue number

CYT-366

Synonyms

GSE, CD152, IDDM12, CELIAC3, CTLA-4.

Introduction

CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
CTLA-4 是免疫球蛋白超家族的成员，编码一种将抑制信号传递给 T 细胞的蛋白质。 该蛋白质包含一个V结构域、一个跨膜结构域和一个胞质尾。 已经表征了编码不同同种型的替代转录剪接变体。 膜结合异构体作为通过二硫键互连的同二聚体发挥作用，而可溶异构体作为单体发挥作用。 该基因的突变与胰岛素依赖型糖尿病、格雷夫斯病、桥本甲状腺炎、乳糜泻、系统性红斑狼疮、甲状腺相关眼眶病和其他自身免疫性疾病有关。

Description

CTLA 4 Human Recombinant produced in E. coli is a single polypeptide chain containing 149 amino acids and having a molecular mass of 15.9 kDa.
CTLA 4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The CTLA 4 solution contains 20mM Tris-HCl buffer , 0.4M urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSKAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSD.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • CTLA 4 Antibody
  • CTLA4 Human, Sf9
  • CTLA4 Mouse
  • CTLA4 Antibody
  • CTLA4 Human, igG-His
  • CTLA4 Human, IgG-His, Active

ProSpec-CTLA4 Human, igG-His/CYT-985

ProSpec CTLA4 Human igG-His,ProSpec CYT-985,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在 Sf9 杆状病毒细胞中产生的 CTLA4 是单条糖基化多肽链，包含 368 个氨基酸，分子量为 40.8kDa。CTLA4 在 C 末端用 239 个氨基酸的 hIgG-His-tag 表达，并通过专有色谱技术进行纯化。

CTLA4 Human, igG-His

Cytotoxic T-Lymphocyte Associated Antigen-4 Human Recombinant, igG-His Tag
CYT-985
ProSpec-CTLA4 Human, igG-His/CYT-985 2µg
ProSpec-CTLA4 Human, igG-His/CYT-985 10µg
ProSpec-CTLA4 Human, igG-His/CYT-985 1mg

Catalogue number

CYT-985

Synonyms

CTLA4, ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12, CD152, Cytotoxic T-Lymphocyte Associated Antigen-4, igG-His Tag.

Introduction

CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
CTLA-4 是免疫球蛋白超家族的成员，编码一种将抑制信号传递给 T 细胞的蛋白质。 该蛋白质包含一个V结构域、一个跨膜结构域和一个胞质尾。 已经表征了编码不同同种型的替代转录剪接变体。 膜结合异构体作为通过二硫键互连的同二聚体发挥作用，而可溶异构体作为单体发挥作用。 该基因的突变与胰岛素依赖型糖尿病、格雷夫斯病、桥本甲状腺炎、乳糜泻、系统性红斑狼疮、甲状腺相关眼眶病和其他自身免疫性疾病有关。

Description

CTLA4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 368 amino acids and having a molecular mass of 40.8kDa .
CTLA4 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 CTLA4 是单条糖基化多肽链，包含 368 个氨基酸，分子量为 40.8kDa。
CTLA4 在 C 末端用 239 个氨基酸的 hIgG-His-tag 表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

CTLA4 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

ADLKAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSDL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • CTLA 4 Human
  • CTLA4 Mouse
  • CTLA4 Human, Sf9
  • CTLA4 Human, IgG-His, Active

ProSpec-CTLA4 Human, IgG-His, Active/CYT-1144

ProSpec CTLA4 Human IgG-His Active,ProSpec CYT-1144,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,用 Jurkat 人急性 T 细胞白血病细胞通过 IL-2 ELISA 测定。 ED50 范围为 ≤ 150 ng/ml，人 B7 1/CD80。

CTLA4 Human, IgG-His, Active

CTLA4 Human Recombinant, igG-His Tag, Active
CYT-1144
ProSpec-CTLA4 Human, IgG-His, Active/CYT-1144 10µg
ProSpec-CTLA4 Human, IgG-His, Active/CYT-1144 50µg
ProSpec-CTLA4 Human, IgG-His, Active/CYT-1144 1mg

Catalogue number

CYT-1144

Synonyms

CTLA4, ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12, CD152, Cytotoxic T-Lymphocyte Associated Antigen-4, igG-His Tag.

Introduction

Cytotoxic T-lymphocyte-associated protein 4 or CTLA4 or CD152 is a receptor that takes part in the immune checkpoint and inhibits immune response. This protein is fundamentally found in regulatory T cells; however, it acts as an enhancer in regular T cells following its activation, this is eminent in cancers. CTLA4 bounds to CD80 or CD86 on the membrane of antigen presenting cells and downregulates transductions.

细胞毒性 T 淋巴细胞相关蛋白 4 或 CTLA4 或 CD152 是一种参与免疫检查点并抑制免疫反应的受体。 这种蛋白质基本上存在于调节性 T 细胞中。 然而，它在被激活后作为常规 T 细胞的增强剂，这在癌症中是显着的。 CTLA4 与抗原呈递细胞膜上的 CD80 或 CD86 结合并下调转导。

Description

CTLA4 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 368 amino acids and having a molecular mass of 40.8kDa.
CTLA4 is fused to a 242 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 CTLA4 Human 是一条糖基化多肽链，包含 368 个氨基酸，分子量为 40.8kDa。
CTLA4 在 C 末端与 242 个氨基酸的 hIgG-His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The CTLA4 solution contains 10% Glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Biological Activity

Determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells.  ED50 range for this is ≤ 150 ng/ml with Human B7 1/CD80.

用 Jurkat 人急性 T 细胞白血病细胞通过 IL-2 ELISA 测定。 ED50 范围为 ≤ 150 ng/ml，人 B7 1/CD80。

Amino acid sequence

ADLKAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG
NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI
DPEPCPDSDL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD
VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN
KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG
QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • CTLA 4 Human
  • CTLA4 Antibody
  • CTLA4 Human, igG-His
  • CTLA4 Human, Sf9
  • CTLA4 Mouse

ProSpec-EBI3 Human, His/CYT-668

ProSpec EBI3 Human, His,ProSpec CYT-668,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,EBI3 在对 Epstein-Barr 病毒感染作出反应的 B 淋巴细胞中具有诱导表达。 EBI3 编码一种属于造血素受体家族的分泌糖蛋白，并与 28 kDa 蛋白异二聚化形成 iIL-27。 EBI3 驱动幼稚 cd4 t 细胞的快速克隆扩增。 EBI3 与 IL-12 强烈协同作用以激活幼稚 cd4 t 细胞的 IFN-γ 产生。 EBI3 通过细胞因子受体 wsx-1/tccr 介导其生物学效应。

EBI3 Human, His

Epstein Barr Virus Induced 3 Human Recombinant, His Tag
CYT-668
ProSpec-EBI3 Human, His/CYT-668 2µg
ProSpec-EBI3 Human, His/CYT-668 10µg
ProSpec-EBI3 Human, His/CYT-668 1mg

Catalogue number

CYT-668

Synonyms

Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, IL27B.

Introduction

EBI3 has an induced expression in B lymphocytes in reaction to Epstein-Barr virus infection. EBI3 encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form iIL-27. EBI3 drives rapid clonal expansion of naive cd4 t-cells. EBI3 strongly synergizes with IL-12 to activate IFN-gamma production of naive cd4 t-cells. EBI3 mediates its biologic effects through the cytokine receptor wsx-1/tccr.
EBI3 在对 Epstein-Barr 病毒感染作出反应的 B 淋巴细胞中具有诱导表达。 EBI3 编码一种属于造血素受体家族的分泌糖蛋白，并与 28 kDa 蛋白异二聚化形成 iIL-27。 EBI3 驱动幼稚 cd4 t 细胞的快速克隆扩增。 EBI3 与 IL-12 强烈协同作用以激活幼稚 cd4 t 细胞的 IFN-γ 产生。 EBI3 通过细胞因子受体 wsx-1/tccr 介导其生物学效应。

Description

EBI3 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 209 amino acids fragment having a molecular weight of 34kDa and fused with a 4.5kDa amino-terminal hexahistidine tag.
The EBI3 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 EBI3 Human Recombinant 是一个单一的、非糖基化的多肽链，包含 209 个氨基酸片段，分子量为 34kDa，并与 4.5kDa 氨基末端六组氨酸标签融合。
EBI3 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

EBI3 protein is supplied in 1xPBS, 50% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
请避免冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • EBI3 Human
  • IL35 Human
  • EBI3 Mouse
  • EBI3 Macaque

ProSpec-EBI3 Macaque/CYT-1036

ProSpec EBI3 Macaque,ProSpec CYT-1036,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,EBI3 在对 Epstein-Barr 病毒感染作出反应的 B 淋巴细胞中具有诱导表达。 EBI3 编码一种属于造血素受体家族的分泌糖蛋白，并与 28 kDa 蛋白异二聚化形成 iIL-27。

EBI3 Macaque

Epstein Barr Virus Induced 3 Macaque Recombinant
CYT-1036
ProSpec-EBI3 Macaque/CYT-1036 5µg
ProSpec-EBI3 Macaque/CYT-1036 20µg
ProSpec-EBI3 Macaque/CYT-1036 1mg

Catalogue number

CYT-1036

Synonyms

IL-27B, IL27B, IL 27-B, EBI-3, Interleukin-27 beta, IL-27 subunit beta, Epstein-Barr virus-induced gene 3 protein homolog, EBI3.

Introduction

EBI3 has an induced expression in B lymphocytes in reaction to Epstein-Barr virus infection. EBI3 encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form iIL-27. EBI3 drives rapid clonal expansion of naive cd4 t-cells. EBI3 strongly synergizes with IL-12 to activate IFN-gamma production of naive cd4 t-cells. EBI3 mediates its biologic effects through the cytokine receptor wsx-1/tccr.

EBI3 在对 Epstein-Barr 病毒感染作出反应的 B 淋巴细胞中具有诱导表达。 EBI3 编码一种属于造血素受体家族的分泌糖蛋白，并与 28 kDa 蛋白异二聚化形成 iIL-27。 EBI3 驱动幼稚 cd4 t 细胞的快速克隆扩增。 EBI3 与 IL-12 强烈协同作用以激活幼稚 cd4 t 细胞的 IFN-γ 产生。 EBI3 通过细胞因子受体 wsx-1/tccr 介导其生物学效应。

Description

EBI3 Macaque Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids and having a molecular mass of 23.4kDa. The EBI3 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 EBI3 Macaque Recombinant 是一条单一的、非糖基化的多肽链，包含 210 个氨基酸，分子量为 23.4kDa。 EBI3 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid .

Solubility

It is recommended to reconstitute the lyophilized EBI3 in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在无菌 18MΩ-cm H2O 中以 100µg/ml 的浓度重构冻干的 EBI3，然后可以将其进一步稀释成其他水溶液。

Stability

Lyophilized EBI3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EBI3 Macaque should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein .

冻干的 EBI3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重新配制后，EBI3 Macaque 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

对于长期储存，建议添加载体蛋白。

Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MRKGPPAALT LPRVQCRAPR YPIAVDCSWT LPPAPNSTSP VSFIATYRFG MAARGHSWPC LQQTPASTSC TIADVRLFSM APYVLNVTAV HPWGSSSSFV PFIAEHIIKP DPPEGVRLSP LAERQLQVQW EPPRSWPFPE IFSLKYWIRY KRQGAARFHQ VGPIEATSFI LRAVRPRARY CVQVAAQDLT DYGELSDWSL PATTPMSPGK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • EBI3 Human
  • EBI3 Mouse
  • EBI3 Human, His

ProSpec-EBI3 Mouse/CYT-621

ProSpec EBI3 Mouse,ProSpec CYT-621,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,冻干的 EBI3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，EBI3 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

EBI3 Mouse

Epstein Barr Virus Induced 3 Mouse Recombinant
CYT-621
ProSpec-EBI3 Mouse/CYT-621 2µg
ProSpec-EBI3 Mouse/CYT-621 10µg
ProSpec-EBI3 Mouse/CYT-621 1mg

Catalogue number

CYT-621

Synonyms

IL-27B, IL27B, IL 27-B, EBI-3, Interleukin-27 beta, IL-27 subunit beta, Epstein-Barr virus-induced gene 3 protein homolog, EBI3.

Introduction

EBI3 has an induced expression in B lymphocytes in reaction to Epstein-Barr virus infection. EBI3 encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form iIL-27. EBI3 drives rapid clonal expansion of naive cd4 t-cells. EBI3 strongly synergizes with IL-12 to activate IFN-gamma production of naive cd4 t-cells. EBI3 mediates its biologic effects through the cytokine receptor wsx-1/tccr.
EBI3 在对 Epstein-Barr 病毒感染作出反应的 B 淋巴细胞中具有诱导表达。 EBI3 编码一种属于造血素受体家族的分泌糖蛋白，并与 28 kDa 蛋白异二聚化形成 iIL-27。 EBI3 驱动幼稚 cd4 t 细胞的快速克隆扩增。 EBI3 与 IL-12 强烈协同作用以激活幼稚 cd4 t 细胞的 IFN-γ 产生。 EBI3 通过细胞因子受体 wsx-1/tccr 介导其生物学效应。

Description

EBI3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 22.9kDa.
The Murine EBI3 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 EBI3 Mouse Recombinant 是一条单一的、非糖基化的多肽链，包含 207 个氨基酸，分子量为 22.9kDa。
Murine EBI3 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

EBI3 was lyophilized from 10mM Sodium Citrate pH-3.

Solubility

It is recommended to reconstitute the lyophilized EBI3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 EBI3，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized EBI3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EBI3 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
冻干的 EBI3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，EBI3 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Purity

Greater than 90% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MALVALSQPR VQCHASRYPV AVDCSWTPLQ APNSTRSTSF IATYRLGVAT QQQSQPCLQR SPQASRCTIP DVHLFSTVPY MLNVTAVHPG GASSSLLAFV AERIIKPDPP EGVRLRTAGQ RLQVLWHPPA SWPFPDIFSL KYRLRYRRRG ASHFRQVGPI EATTFTLRNS KPHAKYCIQV SAQDLTDYGK PSDWSLPGQV ESAPHKP.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • EBI3 Human, His
  • EBI3 Human
  • EBI3 Macaque

ProSpec-Endoglin (27-581) Mouse/CYT-972

ProSpec Endoglin (27-581) Mouse,ProSpec CYT-972,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。对于长期储存，建议添加载体蛋白。避免多次冻融循环。

Endoglin Mouse

Endoglin Mouse Recombinant
CYT-972
ProSpec-Endoglin (27-581) Mouse/CYT-972 1µg
ProSpec-Endoglin (27-581) Mouse/CYT-972 5µg
ProSpec-Endoglin (27-581) Mouse/CYT-972 50µg

Catalogue number

CYT-972

Synonyms

Endoglin, Cell surface MJ7/18 antigen, CD105, Eng, Edg.

Introduction

Endoglin is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex.
The Endoglin protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has been found on endothelial cells, activated macrophages, fibroblasts, and smooth muscle cells. Furthermore, Endoglin has been found to be part of the TGF-beta1 receptor complex. Endoglin thus may be involved in the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beside TGF-beta signaling endoglin may have other functions. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Endoglin expression is regulated during heart development . Experimental mice without the endoglin gene die due to cardiovascular abnormalities.
Endoglin 是一种位于细胞表面的 I 型膜糖蛋白，是 TGF β 受体复合物的一部分。
Endoglin 蛋白由具有二硫键的 180 kDA 同源二聚体组成。 已在内皮细胞、活化的巨噬细胞、成纤维细胞和平滑肌细胞上发现内皮糖蛋白。 此外，已发现内皮糖蛋白是 TGF-β1 受体复合物的一部分。 因此，内皮糖蛋白可能参与 TGF-β1、TGF-β3、激活素 A、BMP-2 和 BMP-7 的结合。 除了 TGF-β 信号转导内皮糖蛋白可能还有其他功能。 据推测，内皮糖蛋白参与影响细胞形态和迁移的细胞骨架组织。 Endoglin 在心血管系统的发育和血管重塑中发挥作用。 内皮糖蛋白的表达在心脏发育过程中受到调节。 没有内皮糖蛋白基因的实验小鼠因心血管异常而死亡。

Description

Endoglin Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 563 amino acids and having a molecular mass of 60.9kDa .
Endoglin is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 Endoglin Mouse Recombinant 是单条糖基化多肽链，包含 563 个氨基酸，分子量为 60.9kDa。
Endoglin 在 C 末端用 8 个氨基酸的 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

Endoglin protein solution containing Phosphate Buffered Saline and 10% glycerol.
含有磷酸盐缓冲液和 10% 甘油的内皮糖蛋白溶液。

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。对于长期储存，建议添加载体蛋白。避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ERVGCDLQPV DPTRGEVTFT TSQVSEGCVA QAANAVREVH VLFLDFPGML SHLELTLQAS KQNGTETQEV FLVLVSNKNV FVKFQAPEIP LHLAYDSSLV IFQGQPRVNI TVLPSLTSRK QILDWAATKG AITSIAALDD PQSIVLQLGQ DPKAPFLCLP EAHKDMGATL EWQPRAQTPV QSCRLEGVSG HKEAYILRIL PGSEAGPRTV TVMMELSCTS GDAILILHGP PYVSWFIDIN HSMQILTTGE YSVKIFPGSK VKGVELPDTP QGLIAEARKL NASIVTSFVE LPLVSNVSLR ASSCGGVFQT TPAPVVTTPP KDTCSPVLLM SLIQPKCGNQ VMTLALNKKH VQTLQCTITG LTFWDSSCQA EDTDDHLVLS SAYSSCGMKV TAHVVSNEVI ISFPSGSPPL RKKVQCIDMD SLSFQLGLYL SPHFLQASNT IELGQQAFVQ VSVSPLTSEV TVQLDSCHLD LGPEGDMVEL IQSRTAKGSC VTLLSPSPEG DPRFSFLLRV YMVPTPTAGT LSCNLALRPS TLSQEVYKTV SMRLNIVSPD LSGKGLEHHH HHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • Endoglin Human, His
  • Endoglin Human
  • Endoglin Human, Sf9
  • Endoglin Mouse

ProSpec-Endoglin Human/CYT-525

ProSpec Endoglin Human,ProSpec CYT-525,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Endoglin 蛋白由具有二硫键的 180 kDA 同源二聚体组成。 已在内皮细胞、活化的巨噬细胞、成纤维细胞和平滑肌细胞上发现内皮糖蛋白。 此外，已发现内皮糖蛋白是 TGF-β1 受体复合物的一部分。 因此，内皮糖蛋白可能参与 TGF-β1、TGF-β3、激活素 A、BMP-2 和 BMP-7 的结合。

Endoglin Human
Endoglin Human Recombinant
CYT-525
ProSpec-Endoglin Human/CYT-525 2µg
ProSpec-Endoglin Human/CYT-525 5µg
ProSpec-Endoglin Human/CYT-525 10µg

Catalogue number

CYT-525

Synonyms

CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.

Introduction

Endoglin is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex.
The Endoglin protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has been found on endothelial cells, activated macrophages, fibroblasts, and smooth muscle cells. Furthermore, Endoglin has been found to be part of the TGF-beta1 receptor complex. Endoglin thus may be involved in the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beside TGF-beta signaling endoglin may have other functions. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Endoglin expression is regulated during heart development . Experimental mice without the endoglin gene die due to cardiovascular abnormalities.
Endoglin 是一种位于细胞表面的 I 型膜糖蛋白，是 TGF β 受体复合物的一部分。
Endoglin 蛋白由具有二硫键的 180 kDA 同源二聚体组成。 已在内皮细胞、活化的巨噬细胞、成纤维细胞和平滑肌细胞上发现内皮糖蛋白。 此外，已发现内皮糖蛋白是 TGF-β1 受体复合物的一部分。 因此，内皮糖蛋白可能参与 TGF-β1、TGF-β3、激活素 A、BMP-2 和 BMP-7 的结合。 除了 TGF-β 信号转导内皮糖蛋白可能还有其他功能。 据推测，内皮糖蛋白参与影响细胞形态和迁移的细胞骨架组织。 Endoglin 在心血管系统的发育和血管重塑中发挥作用。 内皮糖蛋白的表达在心脏发育过程中受到调节。 没有内皮糖蛋白基因的实验小鼠因心血管异常而死亡。

Description

Endoglin Human Recombinant extracellular domain produced in E.Coli is a single, glycosylated, Polypeptide containing 151 amino acids and having a molecular mass of 43 kDa.
The Endoglin is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Endoglin Human Recombinant 胞外结构域是一种单一的糖基化多肽，含有 151 个氨基酸，分子量为 43 kDa。
Endoglin 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid formulation.

Formulation

Endoglin solution in 50mM Tris-Acetate, pH-7.5, 1mM EDTA and 20% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • Endoglin Mouse
  • Endoglin Human, Sf9
  • Endoglin Human, His
  • Endoglin Mouse

ProSpec-Endoglin Human, His/CYT-823

ProSpec Endoglin Human His,ProSpec CYT-823,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,The Endoglin solution contains 20mM Tris-HCl buffer , 1mM DTT, 150mM NaCl and 10% glycerol.

Endoglin Human, His

Endoglin Human Recombinant, His-Tag
CYT-823
ProSpec-Endoglin Human, His/CYT-823 2µg
ProSpec-Endoglin Human, His/CYT-823 10µg
ProSpec-Endoglin Human, His/CYT-823 100µg

Catalogue number

CYT-823

Synonyms

CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.

Introduction

Endoglin is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex.
The Endoglin protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has been found on endothelial cells, activated macrophages, fibroblasts, and smooth muscle cells. Furthermore, Endoglin has been found to be part of the TGF-beta1 receptor complex. Endoglin thus may be involved in the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beside TGF-beta signaling endoglin may have other functions. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Endoglin expression is regulated during heart development . Experimental mice without the endoglin gene die due to cardiovascular abnormalities.
Endoglin 是一种位于细胞表面的 I 型膜糖蛋白，是 TGF β 受体复合物的一部分。
Endoglin 蛋白由具有二硫键的 180 kDA 同源二聚体组成。 已在内皮细胞、活化的巨噬细胞、成纤维细胞和平滑肌细胞上发现内皮糖蛋白。 此外，已发现内皮糖蛋白是 TGF-β1 受体复合物的一部分。 因此，内皮糖蛋白可能参与 TGF-β1、TGF-β3、激活素 A、BMP-2 和 BMP-7 的结合。 除了 TGF-β 信号转导内皮糖蛋白可能还有其他功能。 据推测，内皮糖蛋白参与影响细胞形态和迁移的细胞骨架组织。 Endoglin 在心血管系统的发育和血管重塑中发挥作用。 内皮糖蛋白的表达在心脏发育过程中受到调节。 没有内皮糖蛋白基因的实验小鼠因心血管异常而死亡。

Description

Endoglin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 594 amino acids and having a molecular mass of 64.9 kDa. Endoglin is fused to a 36 amino acid His-tag at N-terminus.
在大肠杆菌中产生的 Endoglin Human Recombinant 是一条含有 594 个氨基酸且分子量为 64.9 kDa 的单一非糖基化多肽链。 Endoglin 在 N 端与 36 个氨基酸的 His 标签融合。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The Endoglin solution contains 20mM Tris-HCl buffer , 1mM DTT, 150mM NaCl and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSETVH CDLQPVGPER DEVTYTTSQV SKGCVAQAPN AILEVHVLFL EFPTGPSQLE LTLQASKQNG TWPREVLLVL SVNSSVFLHL QALGIPLHLA YNSSLVTFQE PPGVNTTELP SFPKTQILEW AAERGPITSA AELNDPQSIL LRLGQAQGSL SFCMLEASQD MGRTLEWRPR TPALVRGCHL EGVAGHKEAH ILRVLPGHSA GPRTVTVKVE LSCAPGDLDA VLILQGPPYV SWLIDANHNM QIWTTGEYSF KIFPEKNIRG FKLPDTPQGL LGEARMLNAS IVASFVELPL ASIVSLHASS CGGRLQTSPA PIQTTPPKDT CSPELLMSLI QTKCADDAMT LVLKKELVAH LKCTITGLTF WDPSCEAEDR GDKFVLRSAY SSCGMQVSAS MISNEAVVNI LSSSSPQRKK VHCLNMDSLS FQLGLYLSPH FLQASNTIEP GQQSFVQVRV SPSVSEFLLQ LDSCHLDLGP EGGTVELIQG RAAKGNCVSL LSPSPEGDPR FSFLLHFYTV PIPKTGTLSC TVALRPKTGS QDQEVHRTVF MRLNIISPDL SGCTSKG

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • Endoglin Human
  • Endoglin Mouse
  • Endoglin Mouse
  • Endoglin Human, Sf9

ProSpec-Endoglin Human, Sf9/CYT-389

ProSpec Endoglin Human Sf9,ProSpec CYT-389,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.

Endoglin Human, Sf9

Endoglin Human Recombinant, Sf9
CYT-389
ProSpec-Endoglin Human, Sf9/CYT-389 2µg
ProSpec-Endoglin Human, Sf9/CYT-389 10µg
ProSpec-Endoglin Human, Sf9/CYT-389 100µg

Catalogue number

CYT-389

Synonyms

CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.

Introduction

Endoglin is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex.
The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts, and smooth muscle cells. Endoglin has been found to be part of the TGF-beta1 receptor complex. It thus may be involved in the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beside TGF-beta signaling endoglin may have other functions. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development . Experimental mice without the endoglin gene die due to cardiovascular abnormalities.
Endoglin 是一种位于细胞表面的 I 型膜糖蛋白，是 TGF β 受体复合物的一部分。
该蛋白质由具有二硫键的 180 kDA 同型二聚体组成。 它存在于内皮细胞、活化的巨噬细胞、成纤维细胞和平滑肌细胞上。 已发现内皮糖蛋白是 TGF-β1 受体复合物的一部分。 因此它可能参与 TGF-β1、TGF-β3、激活素 A、BMP-2 和 BMP-7 的结合。 除了 TGF-β 信号转导内皮糖蛋白可能还有其他功能。 据推测，内皮糖蛋白参与影响细胞形态和迁移的细胞骨架组织。 Endoglin 在心血管系统的发育和血管重塑中发挥作用。 它的表达在心脏发育过程中受到调节。 没有内皮糖蛋白基因的实验小鼠因心血管异常而死亡。

Description

CD105 Human Recombinant extracellular domain produced in baculovirus is a homodimeric, glycosylated, Polypeptide containing 586 amino acids and having a molecular mass of 61 kDa but as a result of glycosylation, migrates at 90 kDa under reducing conditions in -PAGE. The CD105 is fused to a C-terminal His-tag and purified by proprietary chromatographic techniques.
杆状病毒中产生的 CD105 人重组细胞外结构域是一种同型二聚体、糖基化多肽，包含 586 个氨基酸，分子量为 61 kDa，但由于糖基化，在 -PAGE 的还原条件下以 90 kDa 迁移。 CD105 与 C 末端 His 标签融合，并通过专有色谱技术进行纯化。

Source

Sf9 Insect Cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Endoglin was lyophilized from a concentrated sterile solution containing 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized CD-105 in sterile PBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 CD-105 溶解在不低于 100µg/ml 的无菌 PBS 中，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Endoglin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD105 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 Endoglin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CD105 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MDRGTLPLAVALLLASCSLSPTSLAETVHCDLQPVGPERGEVTY TTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVL SVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPI TSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLE GVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLID ANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPL ASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKE LVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNI LSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSE FLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPI PKTGTLSCTVALRPKTGS.

Biological Activity

Measured by its ability to bind with rhTGF-beta RII/Fc in a functional ELISA. Optimal dilutions should be determined by each laboratory for each application.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • Endoglin Mouse
  • Endoglin Human
  • Endoglin Mouse
  • Endoglin Human, His

ProSpec-EREG Human/CYT-609

ProSpec-EREG Human,ProSpec CYT-609,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在大肠杆菌中产生的 Epiregulin Human Recombinant 是单一的、非糖基化的多肽链，包含 49 个氨基酸，分子量为 5.6 kDa。上皮调节蛋白通过专有的色谱技术进行纯化。

EREG Human

Epiregulin Human Recombinant
CYT-609
ProSpec-EREG Human/CYT-609 5µg
ProSpec-EREG Human/CYT-609 25µg
ProSpec-EREG Human/CYT-609 1mg

Catalogue number

CYT-609

Synonyms

EREG, Epiregulin, ER.

Introduction

Epiregulin is part of the EGF family. Epiregulin functions as a ligand of EGFR, as well as a ligand of most members of the ERBB family of tyrosine-kinase receptors.Epiregulin is expressed mostly in the placenta and peripheral blood leukocytes and in specific carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. Epiregulin inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence.
上皮调节蛋白是 EGF 家族的一部分。 上皮调节蛋白作为 EGFR 的配体，以及酪氨酸激酶受体 ERBB 家族大多数成员的配体。上皮调节蛋白主要在胎盘和外周血白细胞以及膀胱、肺、肾和结肠的特定癌中表达 . 上皮调节蛋白刺激角质形成细胞、肝细胞、成纤维细胞和血管平滑肌细胞的增殖。 Epiregulin 抑制几种肿瘤来源的上皮细胞系的生长。 人上皮调节蛋白最初合成为糖基化的 19.0 kDa 跨膜前体蛋白，通过蛋白水解切割加工产生 6.0 kDa 成熟分泌序列。

Description

Epiregulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 49 amino acids and having a molecular mass of 5.6 kDa.
Epiregulin is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Epiregulin Human Recombinant 是单一的、非糖基化的多肽链，包含 49 个氨基酸，分子量为 5.6 kDa。
上皮调节蛋白通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Epiregulin was lyophilized from 0.5mg/ml solution ciontaing 20mM PBS buffer pH-7.4 containing 20mM sodium chloride.

Solubility

It is recommended to reconstitute the lyophilized Epiregulin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的上皮调节蛋白在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Epiregulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Epiregulin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的上皮调节蛋白虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，上皮调节蛋白应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Biological Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is < 2.0 ng/ml, corresponding to a specific activity of > 500,000 units/mg.
通过对鼠 Balb/3T3 细胞增殖的剂量依赖性刺激确定 ED50 < 2.0 ng/ml，对应于 > 500,000 单位/mg 的比活性。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

VAQVSITKC SSDMNGYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • EREG Human, His

ProSpec-FAS Human/CYT-125

ProSpec-FAS Human,ProSpec CYT-125,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在大肠杆菌中产生的 sFas Receptor Human Recombinant 是一条单一的、非糖基化的多肽链，包含 157 个氨基酸，分子量为 17.6kDa。
FAS 通过专有的色谱技术进行纯化。

FAS Human

sFas Receptor Human Recombinant
CYT-125
ProSpec-FAS Human/CYT-125 5µg
ProSpec-FAS Human/CYT-125 20µg
ProSpec-FAS Human/CYT-1251mg

Catalogue number

CYT-125

Synonyms

Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD95, FAS, APT1, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6.

Introduction

Fas and Fas Ligand are members of the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas initiates apoptosis in Fas-bearing cells. The apoptosis mechanism involves the recruitment of pro-caspase 8 through an adaptor molecule named FADD followed by processing of the pro-enzyme to active forms. These active caspases subsequently cleave a variety of cellular substrates leading to the eventual cell death. sFasR is able to inhibit FasL-induced apoptosis by acting as a decoy receptor whicht serves as a sink for FasL. The full length Fas Receptor is a 319 a.a type I transmembrane protein, which contains a 157 a.a extracellular domain, a 17 a.a transmembrane domain, and 145 a.a cytoplasmic domain. The mature human Fas ECD shares 55%, 58%, a.a sequence identity with the mouse, rat, Fas, respectively.
Fas 和 Fas 配体是 TNF 超家族的成员，分别是 I 型和 II 型跨膜蛋白。 FasL 与 Fas 的结合启动了携带 Fas 的细胞的凋亡。 细胞凋亡机制涉及通过称为 FADD 的衔接分子募集半胱天冬酶 8 前体，然后将酶原加工成活性形式。 这些活性半胱天冬酶随后切割多种细胞底物，导致最终的细胞死亡。 sFasR 能够通过充当诱饵受体来抑制 FasL 诱导的细胞凋亡，该诱饵受体充当 FasL 的汇。 全长 Fas 受体是一个 319 a.a 的 I 型跨膜蛋白，它包含一个 157 a.a 的胞外结构域、一个 17 a.a 的跨膜结构域和一个 145 a.a 的胞质结构域。 成熟的人 Fas ECD 分别与小鼠、大鼠、Fas 共享 55%、58%、a.a 序列同一性。

Description

sFas Receptor Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 17.6kDa.
The FAS is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 sFas Receptor Human Recombinant 是一条单一的、非糖基化的多肽链，包含 157 个氨基酸，分子量为 17.6kDa。
FAS 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

FAS protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized FAS in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 FAS，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized FAS although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FAS should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 FAS 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，FAS 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS.

Biological Activity

The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2ng/ml of hFasL.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • FASLG Human
  • FASLG Human, HEK
  • FAS Human, His
  • FAS Antibody
  • FAS Human, Sf9

ProSpec-FAS Human, His/PRO-594

ProSpec-FAS Human His,ProSpec PRO-594,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,ProSpec FAS Human,ProSpec FAS Antibody,CD95 although stable at 10°C for 5 days, should be stored below -18°C.

FAS Human, His

sFas Receptor Human Recombinant, His Tag
PRO-594
ProSpec-FAS Human, His/PRO-594 5µg
ProSpec-FAS Human, His/PRO-594 20µg
ProSpec-FAS Human, His/PRO-594 1mg

Catalogue number

PRO-594

Synonyms

CD95, CD-95, Tumor necrosis factor receptor superfamily member 6, FASLG receptor, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, CD95 antigen, FAS, APT1, FAS1, TNFRSF6, APO-1, FASTM, ALPS1A.

Introduction

FAS / CD95 is a 36kDa transmembrane type I receptor, which belongs to the NF/NGF receptor super-family. It is a potent inducer of apoptosis in cells of the immune system upon interaction with its natural ligand, CD95L. Although the CD95/CD95L system could play a role in tumor regression, tumor cells seem to down-regulate CD95 expression as a mechanism of resistance to CD95L-induced killing by T lymphocytes and NK cells. The Fas antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell line. CD95 is also expressed in a broad panel of non-transformed cells outside the immune system.
FAS/CD95是一种36kDa的跨膜I型受体，属于NF/NGF受体超家族。 在与其天然配体 CD95L 相互作用后，它是免疫系统细胞中的有效细胞凋亡诱导剂。 尽管 CD95/CD95L 系统可以在肿瘤消退中发挥作用，但肿瘤细胞似乎下调 CD95 表达，作为对 CD95L 诱导的 T 淋巴细胞和 NK 细胞杀伤的抗性机制。 Fas 抗原在各种细胞类型的表面表达，包括活化的 T 和 B 淋巴细胞以及 T 淋巴母细胞系。 CD95 也在免疫系统外的一组广泛的非转化细胞中表达。

Description

Recombinant FAS antigen/ CD95 purified from E. coli is a single non-glycosilated polypeptide chain containing amino acids 157-335 of Fas antigen. The recombinant CD95 is fused to C-terminal 6-histidine amino acids. The FAS antigen is purified by proprietary chromatographic techniques.

从大肠杆菌中纯化的重组 FAS 抗原/CD95 是单条非糖基化多肽链，含有 Fas 抗原的 157-335 位氨基酸。 重组 CD95 与 C 末端 6-组氨酸氨基酸融合。 FAS 抗原通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile liquid formulation.

Formulation

10mM Sodium Phosphate, pH 8.0.

Stability

CD95 although stable at 10°C for 5 days, should be stored below -18°C.

Please prevent freeze-thaw cycles.

Purity

Greater than 95% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • FAS Antibody
  • FAS Blocking Antibody
  • FAS Human
  • FASLG Human
  • FASLG Human, HEK
  • FAS Antibody
  • FAS Human, Sf9

ProSpec-FAS Human, Sf9/CYT-1153

ProSpec-FAS Human Sf9,ProSpec CYT-1153,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,FAS或肿瘤坏死因子受体超家族成员6或TNFRSF6，是死亡受体家族的一部分，它是TNF受体蛋白家族的一部分。 TNFRSF6 在调节病毒感染中起着至关重要的作用。

FAS Human, Sf9

sFas Receptor Human Recombinant, Sf9
CYT-1153
2µg
10µg
1mg

Catalogue number

CYT-1153

Synonyms

Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD95, FAS, APT1, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6.

Introduction

FAS or tumor necrosis factor receptor superfamily member 6 or TNFRSF6, is part of the death receptor family, which is part of the TNF receptor protein family. TNFRSF6 has a crucial part in regulating viral infections. FAS protein can be found  in almost all cell types, when its cognate ligand can be found in activated T cells, NK cells & dendritic cells. The regulating protein of FasL & TRAIL on HCMV-infected dendritic enhances direct destruction of activated T lymphocytes. furthermore, subvertneutrophil function in HCMV retinitis can occur when FasL is activated in HCMV infected retinal pigment epithelial cells.

FAS或肿瘤坏死因子受体超家族成员6或TNFRSF6，是死亡受体家族的一部分，它是TNF受体蛋白家族的一部分。 TNFRSF6 在调节病毒感染中起着至关重要的作用。 FAS 蛋白几乎可以在所有细胞类型中找到，而它的同源配体可以在活化的 T 细胞、NK 细胞和树突状细胞中找到。 HCMV 感染树突上的 FasL 和 TRAIL 调节蛋白增强了活化 T 淋巴细胞的直接破坏。 此外，当在 HCMV 感染的视网膜色素上皮细胞中激活 FasL 时，可能会发生 HCMV 视网膜炎中的亚中性粒细胞功能。

Description

FAS Human Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of  17.7KDa.
FAS  is fused to a 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

在杆状病毒中产生的 FAS Human Recombinant 是单条糖基化多肽链，包含 156 个氨基酸，分子量为 17.7KDa。
FAS 在 C 端与 8 个氨基酸的 His-Tag 融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

FAS protein contains 10% glycerol and Phosphate-Buffered Saline .

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRSNLE HHHHHH

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • FAS Human
  • FAS Human, His

ProSpec-FASLG Human/CYT-031

FASLG Human

FAS Ligand Human Recombinant
CYT-031
ProSpec-FASLG Human/CYT-031 5µg
ProSpec-FASLG Human/CYT-031 20µg
ProSpec-FASLG Human/CYT-031 1mg

Catalogue number

CYT-031

Synonyms

Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178, FASLG, APT1LG1, CD95L, TNFSF6, ALPS1B.

Introduction

The type II transmembrane protein FASLG is a member of the tumor necrosis factor superfamily. A fas ligand/receptor interaction has a significant part in the regulation of the immune system and the advancement of cancer. FASLG is expressed on the activated T cell surface as a nondisulfidelinked homotrimer. FASLG binding to Fas/CD95/TNFRSF6 on a nearby cell prompts apoptosis in the Fas expressing cell. FASLG is released from the cell surface by metalloproteinases as a soluble molecule that stays trimeric and is able to bind with Fas, but its capability to activate apoptosis is radically reduced. In addition, FASLG binds to DcR3 – a soluble trap receptor with no signal transduction capabilities. Flawed Fas-mediated apoptosis causes oncogenesis in addition to drug resistance in existing tumors. Constitutive expression of FASLG in a variety of tumors enables their immune evasion. Both mouse and human FASLG are active on mouse and human cells.
II型跨膜蛋白FASLG是肿瘤坏死因子超家族的成员。 fas 配体/受体相互作用在免疫系统调节和癌症进展中具有重要作用。 FASLG 在活化的 T 细胞表面以非二硫键同源三聚体的形式表达。 FASLG 与附近细胞上的 Fas/CD95/TNFRSF6 结合会促使表达 Fas 的细胞发生凋亡。 FASLG 作为可溶性分子通过金属蛋白酶从细胞表面释放，保持三聚体并能够与 Fas 结合，但其激活细胞凋亡的能力从根本上降低。 此外，FASLG 与 DcR3 结合 – 一种没有信号转导能力的可溶性陷阱受体。 除了现有肿瘤的耐药性外，有缺陷的 Fas 介导的细胞凋亡还会导致肿瘤发生。 FASLG 在多种肿瘤中的组成型表达使其能够逃避免疫。 小鼠和人类 FASLG 都对小鼠和人类细胞具有活性。

Description

FASLG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 19.6kDa.
FASLG is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 FASLG Human Recombinant 是一条单一的、非糖基化的多肽链，包含 173 个氨基酸，分子量为 19.6kDa。
FASLG 在 N 末端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

FASLG protein solution containing 20mM Tris-HCl buffer , 0.4M urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MQIGHPSPPP EKKELRKVAH LTGKSNSRSM PLEWEDTYGI VLLSGVKYKK GGLVINETGL YFVYSKVYFR GQSCNNLPLS HKVYMRNSKY PQDLVMMEGK MMSYCTTGQM WARSSYLGAV FNLTSADHLY VNVSELSLVN FEESQTFFGL YKL.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • FAS Human
  • FASLG Human, HEK
  • FAS Human, His
  • FAS Antibody

ProSpec-FASLG Human, HEK/CYT-051,FAS Ligand Human Recombinant, HEK

ProSpec-FASLG Human, HEK,ProSpec CYT-051,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,II型跨膜蛋白FASLG是肿瘤坏死因子超家族的成员。 fas 配体/受体相互作用在免疫系统调节和癌症进展中具有重要作用。 FASLG 在活化的 T 细胞表面以非二硫键同源三聚体的形式表达。

FASLG Human, HEK

FAS Ligand Human Recombinant, HEK
CYT-051
ProSpec-FASLG Human, HEK/CYT-051 2µg
ProSpec-FASLG Human, HEK/CYT-051 10µg
ProSpec-FASLG Human, HEK/CYT-051 1mg

Catalogue number

CYT-051

Synonyms

Fas ligand , APT1LG1, FASL, TNFSF6, CD178, tumor necrosis factor superfamily member 6, Apoptosis antigen ligand, Fas antigen ligand, APTL, CD95-L.

Introduction

The type II transmembrane protein FASLG is a member of the tumor necrosis factor superfamily. A fas ligand/receptor interaction has a significant part in the regulation of the immune system and the advancement of cancer. FASLG is expressed on the activated T cell surface as a nondisulfidelinked homotrimer. FASLG binding to Fas/CD95/TNFRSF6 on a nearby cell prompts apoptosis in the Fas expressing cell. FASLG is released from the cell surface by metalloproteinases as a soluble molecule that stays trimeric and is able to bind with Fas, but its capability to activate apoptosis is radically reduced. In addition, FASLG binds to DcR3 – a soluble trap receptor with no signal transduction capabilities. Flawed Fas-mediated apoptosis causes oncogenesis in addition to drug resistance in existing tumors. Constitutive expression of FASLG in a variety of tumors enables their immune evasion. Both mouse and human FASLG are active on mouse and human cells.
II型跨膜蛋白FASLG是肿瘤坏死因子超家族的成员。 fas 配体/受体相互作用在免疫系统调节和癌症进展中具有重要作用。 FASLG 在活化的 T 细胞表面以非二硫键同源三聚体的形式表达。 FASLG 与附近细胞上的 Fas/CD95/TNFRSF6 结合会促使表达 Fas 的细胞发生凋亡。 FASLG 作为可溶性分子通过金属蛋白酶从细胞表面释放，保持三聚体并能够与 Fas 结合，但其激活细胞凋亡的能力从根本上降低。 此外，FASLG 与 DcR3 结合 – 一种没有信号转导能力的可溶性陷阱受体。 除了现有肿瘤的耐药性外，有缺陷的 Fas 介导的细胞凋亡还会导致肿瘤发生。 FASLG 在多种肿瘤中的组成型表达使其能够逃避免疫。 小鼠和人类 FASLG 都对小鼠和人类细胞具有活性。

Description

Recombinant Human FAS Ligand produced in HEK293 cells is a polypeptide chain containing 147 amino acids .
FASLG is fused to a 6 amino acid His-tag at N-terminus and purified by proprietary chromatographic techniques.
HEK293细胞产生的重组人FAS配体是一条含有147个氨基酸的多肽链。
FASLG 在 N 末端与 6 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

HEK293 cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The FASLG solution contains 1xPBS.

Stability

FASLG Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
FASLG Human Recombinant 虽然在 4°C 下稳定 1 周，但应在 -18°C 以下储存。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by SEC-HPLC.
Analysis by -PAGE.

Biological Activity

Fas ligand is biologically active as determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The expected ED50< 10 ng/ml, corresponding to a specific activity of 1×105 units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • FAS Human
  • FASLG Human
  • FAS Human, His
  • FAS Antibody

ProSpec-Flt3 Ligand Human/CYT-331,Flt3 Ligand Human Recombinant

ProSpec-Flt3 Ligand Human/CYT-331,Flt3 Ligand Human Recombinant,建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Flt3-L，然后可以进一步稀释成其他水溶液。

Flt3 Ligand Human

Flt3 Ligand Human Recombinant
CYT-331
ProSpec-Flt3 Ligand Human/CYT-331 2µg
ProSpec-Flt3 Ligand Human/CYT-331 10µg
ProSpec-Flt3 Ligand Human/CYT-331 1mg

Catalogue number

CYT-331

Synonyms

Fms-Related Tyrosine Kinase 3 Ligand, Flt3 Ligand, Flt-3 Ligand, SL Cytokine.

Introduction

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.

Description

Flt3-Ligand Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of approximately 17.6kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized with no additives.

Solubility

It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Flt3-L，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt3-Ligand 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA.

Biological Activity

The ED50 as calculated by the dose-dependent stimulation of the proliferation of human AML5 cells is less than 1.0 ng/ml, corresponding to a Specific Activity of 1.0×106 IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

References

1.Title:Identification of Y589 and Y599 in the
juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2.
Publication:Published online before printMay 9, 2006, doi:10.1182/blood-2005-07-008896
Submitted: July 14, 2005Accepted: April 26, 2006 BloodSeptember 1, 2006vol. 108no. 51542-1550.
Link:http://bloodjournal.hematologylibrary.org/content/108/5/1542.full2. Title:A role of Gab2 association in Flt3 ITD mediated Stat5 phosphorylation and cell survival.
Publication:Received 2 February 2009; accepted for publication 30 March 2009 Correspondence: Lars Ro¨nnstrand,Experimental Clinical Chemistry, WallenbergLaboratory, Entr. 46, 5th fl, Malmo¨ University Hospital, SE-20502 Malmo¨, Sweden.E-mail: lars.ronnstrand@med.lu.se;http://www.expklkemi.mas.lu.se
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2141.2009.07725.x/pdf

Related Products

  • Flt3 Ligand Mouse, Sf9
  • Flt3 Ligand Mouse
  • Flt3 Ligand Human, Sf9
  • Flt3-Ligand Rhesus Macaque
  • Flt3 Ligand Human, HEK
  • Flt3 Ligand Porcine

ProSpec-Flt3 Ligand Human, HEK/CYT-706,Flt3-Ligand Human Recombinant, HEK derived

ProSpec-Flt3 Ligand Human HEK,ProSpec CYT-706,Flt3-Ligand Human Recombinant HEK derived,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,Greater than 95.0% as determined by: Analysis by RP-HPLC. Analysis by -PAGE.

Flt3 Ligand Human, HEK

Flt3-Ligand Human Recombinant, HEK derived
CYT-706
ProSpec-Flt3 Ligand Human, HEK/CYT-706 2µg
ProSpec-Flt3 Ligand Human, HEK/CYT-706 10µg
ProSpec-Flt3 Ligand Human, HEK/CYT-706 0.1mg

Catalogue number

CYT-706

Synonyms

Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Introduction

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Description

Flt3-Ligand Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 24-30kDa due to glycosylation. The Flt3-Ligand is purified by proprietary chromatographic techniques.

在 HEK 细胞中产生的 Flt3-Ligand Human Recombinant 是一种糖基化单体，由于糖基化，其分子量范围为 24-30kDa。 Flt3-Ligand 通过专有的色谱技术进行纯化。

Source

HEK293 .

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Flt3-Ligand was lyophilized from a 0.2µm filtered solution containing 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Flt3-Ligand，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.
冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt3-Ligand 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。 请防止冻融循环。

Purity

Greater than 95.0% as determined by: Analysis by RP-HPLC. Analysis by -PAGE.

Amino acid sequence

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP.

Biological Activity

The activity was determined by the dose- dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5. The ED50 is 0.56ng/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • Flt3 Ligand Mouse, Sf9
  • Flt3 Ligand Mouse
  • Flt3 Ligand Human
  • Flt3 Ligand Human, Sf9
  • Flt3-Ligand Rhesus Macaque

ProSpec-Flt3 Ligand Human, Sf9/CYT-409

ProSpec-Flt3 Ligand Human, Sf9,ProSpec CYT-409,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt3-L 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。

Flt3 Ligand Human, Sf9

Flt3 Ligand Human Recombinant, Sf9
CYT-409
ProSpec-Flt3 Ligand Human, Sf9/CYT-409 2µg
ProSpec-Flt3 Ligand Human, Sf9/CYT-409 10µg
ProSpec-Flt3 Ligand Human, Sf9/CYT-409 0.1mg

Catalogue number

CYT-409

Synonyms

Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Introduction

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Description

Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on -PAGE at 19kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.
在 Sf9 昆虫细胞中产生的 Flt3-Ligand Human Recombinant 是一种糖基化的多肽链，含有 155 个氨基酸，并在 -PAGE 上以 19kDa 迁移。
Flt3-Ligand 通过专有的色谱技术进行纯化。

Source

Baculovirus Sf9 cells.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized with no additives.

Solubility

It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-L should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt3-L 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.

Biological Activity

The ED50, calculated by the dose-dependent stimulation of the proliferation of human MUTZ-2 cells is < 3.0 ng/ml.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard.
蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.42 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. RP-HPLC 分析，使用标准 Flt3-Ligand 作为参考标准。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • Flt3 Ligand Human, HEK
  • Flt3-Ligand Rhesus Macaque
  • Flt3 Ligand Mouse, Sf9
  • Flt3 Ligand Human
  • Flt3 Ligand Mouse

ProSpec-Flt3 Ligand Mouse/CYT-340

ProSpec Flt3 Ligand Mouse,ProSpec CYT-340,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt-3 配体应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。 请防止冻融循环。

Flt3 Ligand Mouse

Flt3 Ligand Mouse Recombinant
CYT-340
ProSpec-Flt3 Ligand Mouse/CYT-340 2µg
ProSpec-Flt3 Ligand Mouse/CYT-340 10µg
ProSpec-Flt3 Ligand Mouse/CYT-340 1mg

Catalogue number

CYT-340

Synonyms

Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Introduction

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Description

Flt3-Ligand Mouse Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 163 amino acids and having a molecular mass of 18.6kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Flt3-Ligand Mouse Recombinant 是一种非糖基化的多肽链，含有 163 个氨基酸，分子量为 18.6kDa。 Flt3-Ligand 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The FLT3L Mouse protein was lyophilized from sterile filtered solution containing 10mM sodium phosphate, pH 7.5.

Solubility

It is recommended to reconstitute the lyophilized Flt3l Mouse recombinant in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt-3 Ligand should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.

冻干的 Flt3-Ligand 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt-3 配体应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。 请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ.

Biological Activity

The ED50, calculated by the dose-dependent proliferation of mouse AML5 cells is 4.96ng/ml, corresponding to a specific activity of 2.0×105 units/mg.

通过小鼠 AML5 细胞的剂量依赖性增殖计算的 ED50 为 4.96ng/ml，对应于 2.0×105 单位/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • Flt3 Ligand Human
  • Flt3 Ligand Human, Sf9
  • Flt3-Ligand Rhesus Macaque
  • Flt3 Ligand Human, HEK
  • Flt3 Ligand Mouse, Sf9

ProSpec-Flt3 Ligand Mouse, Sf9/CYT-910

ProSpec-Flt3 Ligand Mouse Sf9,ProSpec CYT-910,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Flt3 Ligand Mouse, Sf9

Flt3 Ligand Mouse Recombinant, Sf9
CYT-910
ProSpec-Flt3 Ligand Mouse, Sf9/CYT-910 1µg
ProSpec-Flt3 Ligand Mouse, Sf9/CYT-910 5µg
ProSpec-Flt3 Ligand Mouse, Sf9/CYT-91050µg

Catalogue number

CYT-910

Synonyms

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, lt3lgF, Flt3l.

Introduction

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.

FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Description

Flt3 Ligand produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain and fused to a 6 aa His Tag at C-terminus containing a total of 169 amino acids and having a molecular mass of 19.3kDa.
Flt3 Ligand shows multiple bands between 18-28kDa on -PAGE, reducing conditions and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 Flt3 配体是一条糖基化多肽链，在 C 端与一个 6 个氨基酸的 His 标签融合，共含有 169 个氨基酸，分子量为 19.3kDa。
Flt3 Ligand 在 -PAGE 上显示出 18-28kDa 之间的多个条带，还原条件并通过专有色谱技术纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

Flt3 Ligand protein solution contains Phosphate buffered saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

GTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQHHHHHH

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • Flt3-Ligand Rhesus Macaque
  • Flt3 Ligand Human, HEK
  • Flt3 Ligand Mouse
  • Flt3 Ligand Human
  • Flt3 Ligand Human, Sf9

ProSpec-Flt3-Ligand Rhesus Macaque/CYT-157

ProSpec-Flt3-Ligand Rhesus Macaque,ProSpec CYT-157,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Flt3-Ligand Rhesus Macaque

Flt3-Ligand Rhesus Macaque Recombinant
CYT-157
ProSpec-Flt3-Ligand Rhesus Macaque/CYT-157 2µg
ProSpec-Flt3-Ligand Rhesus Macaque/CYT-157 10µg
ProSpec-Flt3-Ligand Rhesus Macaque/CYT-157 1mg

Catalogue number

CYT-157

Synonyms

Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Introduction

FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
FLT3 配体是 fl 细胞因子的受体，具有酪氨酸蛋白激酶活性和调节早期造血细胞增殖的生长因子。 Flt3-配体与其他脑脊液和白细胞介素协同作用以诱导生长和分化。

Description

Flt3 Ligand Rhesus Macaque Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids and having a molecular mass of 18.0kDa.
The Flt3 Ligand Rhesus Macaque is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Flt3 Ligand Rhesus Macaque Recombinant 是一种单一的、非糖基化的多肽链，包含 159 个氨基酸，分子量为 18.0kDa。
Flt3 Ligand Rhesus Macaque 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Flt3 Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 Flt3 配体在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Flt3 Ligand Rhesus Macaque although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3 Ligand Rhesus Macaque should be stored at 4°C between 2-7 days and for future use below -18°C.
冻干的 Flt3 配体恒河猴虽然在室温下稳定 3 周，但应在 -18°C 以下干燥储存。 复溶后，Flt3 Ligand Rhesus Macaque 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by -PAGE.

Amino acid sequence

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVPSNLQD EELCGALWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQHP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPRSPGALEA TALTAPQRP

Biological Activity

The ED50 as calculated by the dose-dependant stimulation of the proliferation of human AML5 cells is less than 1.0ng/ml, corresponding to a specific activity of 1,000,000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • Flt3 Ligand Human, Sf9
  • Flt3 Ligand Human, HEK
  • Flt3 Ligand Mouse, Sf9
  • Flt3 Ligand Mouse
  • Flt3 Ligand Human

ProSpec-FST Human/CYT-232

ProSpec-FST Human,ProSpec CYT-232,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,在大肠杆菌中产生的 Follistatin Human Recombinant 是一条单一的、非糖基化的多肽链，包含 288 个氨基酸，总分子量为 31.5kDa。
FST 通过专有的色谱技术进行纯化。

FST Human

Follistatin Human Recombinant
CYT-232
ProSpec-FST Human/CYT-232 5µg
ProSpec-FST Human/CYT-232 20µg
ProSpec-FST Human/CYT-232 1mg

Catalogue number

CYT-232

Synonyms

FST, FS

Introduction

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin  functions as an ACTV antagonist. specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone .

卵泡抑素是一种单链性腺蛋白，可特异性抑制促卵泡激素的释放。 单个 FST 基因编码两种同种型，FST317 和 FST344，分别含有 317 和 344 个氨基酸，由前体 mRNA 的可变剪接产生。 在一项研究中，测试了 37 个候选基因与 150 个家庭的多囊卵巢综合征或高雄激素血症的关联和关联，发现了 PCOS 和卵泡抑素之间关联的证据。 卵泡抑素起到 ACTV 拮抗剂的作用。 垂体促卵泡激素的生物合成和分泌的特异性抑制剂。

Description

Follistatin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kDa.
The FST is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Follistatin Human Recombinant 是一条单一的、非糖基化的多肽链，包含 288 个氨基酸，总分子量为 31.5kDa。
FST 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Follistatin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 Follistatin，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FST should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 Follistatin 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，FST 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Asn-Cys-Trp-Leu.

Biological Activity

The activity is determined by the ability to neutralize ACTV inhibitory effect of mouse MPC-11 cells. The expected ED50 is 100-400ng/ml, corresponding to a Specific Activity of 2,500-10,000units/mg in the presence of 7.5ng/ml ACTV A.

活性取决于中和小鼠 MPC-11 细胞的 ACTV 抑制作用的能力。 预期的 ED50 为 100-400ng/ml，对应于 7.5ng/ml ACTV A 的比活性为 2,500-10,000units/mg。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • FST Human, Sf9
  • FST Human, His
  • FST Mouse

ProSpec-FST Human, His/CYT-029

ProSpec-FST Human His,ProSpec CYT-029,ProSpec代理,ProSpec产品,ProSpec产品目录,ProSpec Cytokines,卵泡抑素是一种单链性腺蛋白，可特异性抑制促卵泡激素的释放。 单个 FST 基因编码两种同种型，FST317 和 FST344，分别含有 317 和 344 个氨基酸，由前体 mRNA 的可变剪接产生。

FST Human, His

Follistatin Human Recombinant, His Tag
CYT-029
ProSpec-FST Human, His/CYT-029 2µg
ProSpec-FST Human, His/CYT-029 10µg
ProSpec-FST Human, His/CYT-029 1mg

Catalogue number

CYT-029

Synonyms

FST, FS, Activin-binding protein.

Introduction

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone .
卵泡抑素是一种单链性腺蛋白，可特异性抑制促卵泡激素的释放。 单个 FST 基因编码两种同种型，FST317 和 FST344，分别含有 317 和 344 个氨基酸，由前体 mRNA 的可变剪接产生。 在一项研究中，测试了 37 个候选基因与 150 个家庭的多囊卵巢综合征或高雄激素血症的关联和关联，发现了 PCOS 和卵泡抑素之间关联的证据。 卵泡抑素直接与激活素结合并作为激活素拮抗剂发挥作用。 垂体促卵泡激素的生物合成和分泌的特异性抑制剂。

Description

FST His Protein is 36.0 kDa protein containing 325 amino acid residues of the FST His and the 10 aa N-Terminal His-tag.

Source

E. coli.

Formulation

FST His Tag was filtered and lyophilized from 0.5mg/ml supplied in 20mM TRIS and 20mM NaCl, pH 7.5.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized FST His at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted FST His can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 FST His 储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重构的 FST His 可在 4°C 下储存一段有限的时间； 在 4°C 下两周后它没有显示任何变化。

Amino acid sequence

MKHHHHHHAS GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCNSI SEDTEEEEED EDQDYSFPIS SILEW.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • FST Mouse
  • FST Human
  • FST Human, Sf9