标签： ProSpec产品

ProSpec-4 1BBL Human

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec-4 1BBL Human,4-1BBL 是一种跨膜细胞因子，是肿瘤坏死因子配体家族的一部分。 4-1BBL 是一种双向信号传感器，可作为 TNFRSF9 的配体，TNFRSF9 是 T 淋巴细胞中的一种共刺激受体分子。

  • 4 1BBL Human

  • 4-1BB Ligand Human Recombinant
  • CYT-149

Catalogue number

CYT-149

Synonyms

CD137L, CD137-L, 4-1BBL, 4-1BB Ligand, TNFSF9, Tumor Necrosis Factor Superfamily Member 9.

Introduction

4-1BBL is a transmembrane cytokine that is part of the tumor necrosis factor ligand family. 4-1BBL is a bidirectional signal transducer that performs as a ligand for TNFRSF9, which is a costimulatory receptor molecule in T lymphocytes. TNFSF9 and its TNFRSF9 take part in the antigen presentation development and in the generation of cytotoxic T cells. 4-1BBR is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. TNFSF9 reactivates anergic T lymphocytes as well as promoting T lymphocyte proliferation. 4-1BB Ligand is needed for the optimal CD8 responses in CD8 T cells. 4-1BBL is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. The interaction of 4-1BB with TNFRSF9 strongly regulates immunity and has been proposed to preferentially control T cell responses based on studies in various murin.
4-1BBL 是一种跨膜细胞因子，是肿瘤坏死因子配体家族的一部分。 4-1BBL 是一种双向信号传感器，可作为 TNFRSF9 的配体，TNFRSF9 是 T 淋巴细胞中的一种共刺激受体分子。 TNFSF9 及其 TNFRSF9 参与抗原呈递发展和细胞毒性 T 细胞的产生。 4-1BBR 在静息 T 淋巴细胞中不存在，但在抗原刺激后迅速表达。 TNFSF9 重新激活无反应性 T 淋巴细胞并促进 T 淋巴细胞增殖。 CD8 T 细胞中的最佳 CD8 反应需要 4-1BB 配体。 4-1BBL 在癌细胞系中表达，被认为与 T 细胞-肿瘤细胞相互作用有关。 4-1BBL 由活化的 B 细胞、巨噬细胞、树突细胞、活化的 T 细胞、神经元和星形胶质细胞表达。 4-1BB 与 TNFRSF9 的相互作用强烈调节免疫，并且基于对各种小鼠的研究已被提议优先控制 T 细胞反应。

Description

4 1BBL Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids and having a molecular mass of 19.5kDa.
The 4 1BBL is purified by proprietary chromatographic techniques.
4 由大肠杆菌产生的 1BBL Human Recombinant 是一条单一的、非糖基化的多肽链，包含 184 个氨基酸，分子量为 19.5kDa。
4 1BBL 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized 4 1BBL in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized 4 1BBL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution 4 1BBL should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
冻干的 4 1BBL 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后 4 1BBL 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE

Biological Activity

Fully biologically active when compared to standard. Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is 5-10ng/ml corresponding to a Specific Activity of 100,000-200,000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-4 1BBL Human, His

ProSpec-4 1BBL Human, His,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,4-1BBL 是一种跨膜细胞因子，是肿瘤坏死因子配体家族的一部分。 4-1BBL 是一种双向信号传感器，可作为 TNFRSF9 的配体，TNFRSF9 是 T 淋巴细胞中的一种共刺激受体分子。

 

     4 1BBL Human, His

  • 4-1BB Ligand Human Recombinant, His Tag
  • CYT-649

Catalogue number

CYT-649

Synonyms

CD137L, CD137-L, 4-1BBL, 4-1BB Ligand, TNFSF9, Tumor Necrosis Factor Superfamily Member 9.

Introduction

4-1BBL is a transmembrane cytokine that is part of the tumor necrosis factor ligand family. 4-1BBL is a bidirectional signal transducer that performs as a ligand for TNFRSF9, which is a costimulatory receptor molecule in T lymphocytes. TNFSF9 and its TNFRSF9 take part in the antigen presentation development and in the generation of cytotoxic T cells. 4-1BBR is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. TNFSF9 reactivates anergic T lymphocytes as well as promoting T lymphocyte proliferation. 4-1BB Ligand is needed for the optimal CD8 responses in CD8 T cells. 4-1BBL is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. The interaction of 4-1BB with TNFRSF9 strongly regulates immunity and has been proposed to preferentially control T cell responses based on studies in various murin
4-1BBL 是一种跨膜细胞因子，是肿瘤坏死因子配体家族的一部分。 4-1BBL 是一种双向信号传感器，可作为 TNFRSF9 的配体，TNFRSF9 是 T 淋巴细胞中的一种共刺激受体分子。 TNFSF9 及其 TNFRSF9 参与抗原呈递发展和细胞毒性 T 细胞的产生。 4-1BBR 在静息 T 淋巴细胞中不存在，但在抗原刺激后迅速表达。 TNFSF9 重新激活无反应性 T 淋巴细胞并促进 T 淋巴细胞增殖。 CD8 T 细胞中的最佳 CD8 反应需要 4-1BB 配体。 4-1BBL 在癌细胞系中表达，被认为与 T 细胞-肿瘤细胞相互作用有关。 4-1BBL 由活化的 B 细胞、巨噬细胞、树突细胞、活化的 T 细胞、神经元和星形胶质细胞表达。 4-1BB 与 TNFRSF9 的相互作用强烈调节免疫，并已根据对各种小鼠的研究提出优先控制 T 细胞反应

Description

TNFSF9 Human Recombinant fused to 37 amino acids His tag at N-terminus produced in E.Coli is a single, non-glycosylated polypeptide chain containing 222 amino acids and having a molecular mass of 23.8 kDa.
The TNFSF9 37 aa His tag fusion protein is purified by proprietary chromatographic techniques.
TNFSF9 Human Recombinant 融合了 37 个氨基酸 在 E.Coli 中产生的 N 端 His 标签是一条含有 222 个氨基酸且分子量为 23.8 kDa 的单一非糖基化多肽链。
TNFSF9 37 aa His 标签融合蛋白通过专有色谱技术纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

Recombinant 4-1BBL solution contains 20mM Tris-HCl buffer pH-8, 100mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by -PAGE

Amino acid sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTGGLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL GVHLHTEARA RHAWQLTQGA  TVLGLFRVTP EIPAGLPSPR SE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-4 1BBR Human

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,该基因编码的蛋白质是 TNF 受体超家族的成员。 该受体有助于 T 细胞的克隆扩增、存活和发育。 它还可以诱导外周单核细胞增殖，增强 TCR/CD3 触发激活诱导的 T 细胞凋亡，并调节 CD28 共刺激以促进 Th1 细胞反应。

 

  • 4 1BBR Human

  • 4-1BB Receptor Human Recombinant
  • CYT-463
  • 4 1BBR Human 
    4 1BBR Human 
    4 1BBR Human 

Catalogue number

CYT-463

Synonyms

Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor T-cell, antigen 4-1BB homolog, T-cell antigen ILA, CD137 antigen, CDw137, ILA, 4-1BB, MGC2172, 4-1BBR, TNFRSF9.

Introduction

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
该基因编码的蛋白质是 TNF 受体超家族的成员。 该受体有助于 T 细胞的克隆扩增、存活和发育。 它还可以诱导外周单核细胞增殖，增强 TCR/CD3 触发激活诱导的 T 细胞凋亡，并调节 CD28 共刺激以促进 Th1 细胞反应。 这种受体的表达是由淋巴细胞激活诱导的。 已显示 TRAF 衔接蛋白与该受体结合并转导导致 NF-kappaB 激活的信号。

Description

4-1BB Soluble Receptor Recombinant Human also called Tumor necrosis factor receptor superfamily member 9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids, having a molecular mass of 17718 Dalton and containing the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor. The 4-1BB Receptor is purified by proprietary chromatographic techniques.
4-1BB Soluble Receptor Recombinant Human 也称为肿瘤坏死因子受体超家族成员 9 由大肠杆菌产生，是一个单一的、非糖基化的多肽链，含有 167 个氨基酸，分子量为 17718 道尔顿，含有富含半胱氨酸的 TNFR 样 4-1BB 受体的细胞外结构域。 4-1BB 受体通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a concentrated solution in water containing no additives.
从不含添加剂的水中浓缩溶液冻干而成。

Solubility

It is recommended to reconstitute the lyophilized 4-1BB Receptor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议将冻干的 4-1BB 受体在不低于 100μg/ml 的无菌 18MΩ-cm H2O 中复溶，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized 4-1BB Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution 4-1BBR should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.
冻干的 4-1BB 受体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，4-1BBR 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。 请防止冻融循环。

Purity

Greater than 98.0% as determined by: Analysis by RP-HPLC. Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Arg-Thr-Arg.

Biological Activity

The activity was determined by the inhibition of 4-1BB ligand mediated stimulation of IL-8 production by human PBMC. Results: 90% inhibition using 1µg for both 4-1BB ligand and 4-1BB receptor.
通过抑制 4-1BB 配体介导的人 PBMC 产生 IL-8 的刺激来确定活性。 结果：使用 1µg 对 4-1BB 配体和 4-1BB 受体有 90% 的抑制作用。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-4 1BBR Human, His

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,4 1BBR Human, His,ProSpec-4 1BBR Human, His,在大肠杆菌中产生的 4-1BBR Human Recombinant 是单一的非糖基化多肽链，包含 193 个氨基酸，分子量为 20kDa。

 

4 1BBR Human, His

4-1BB Receptor Human Recombinant, His Tag
CYT-137

4 1BBR Human, His 5µg
4 1BBR Human, His 20µg
4 1BBR Human, His 1mg

Catalogue number

CYT-137

Synonyms

Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor T-cell, antigen 4-1BB homolog, T-cell antigen ILA, CD137 antigen, CDw137, ILA, 4-1BB, MGC2172, 4-1BBR, TNFRSF9.
肿瘤坏死因子受体超家族成员9、4-1BB配体受体T细胞、抗原4-1BB同源物、T细胞抗原ILA、CD137抗原、CDw137、ILA、4-1BB、MGC2172、4-1BBR、TNFRSF9。

Introduction

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
该基因编码的蛋白质是 TNF 受体超家族的成员。 该受体有助于 T 细胞的克隆扩增、存活和发育。 它还可以诱导外周单核细胞增殖，增强 TCR/CD3 触发激活诱导的 T 细胞凋亡，并调节 CD28 共刺激以促进 Th1 细胞反应。 这种受体的表达是由淋巴细胞激活诱导的。 已显示 TRAF 衔接蛋白与该受体结合并转导导致 NF-kappaB 激活的信号。

Description

4-1BBR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 193 amino acids and having a molecular mass of 20kDa.
4-1BBR is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 4-1BBR Human Recombinant 是单一的非糖基化多肽链，包含 193 个氨基酸，分子量为 20kDa。
4-1BBR 在 N 末端与 24 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

4-1BBR protein solution containing 20mM Tris-HCl buffer , 0.1M NaCl, 10% glycerol and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMFERTRS LQDPCSNCPA GTFCDNNRNQ ICSPCPPNSF SSAGGQRTCD ICRQCKGVFR TRKECSSTSN AECDCTPGFH CLGAGCSMCE QDCKQGQELT KKGCKDCCFG TFNDQKRGIC RPWTNCSLDG KSVLVNGTKE RDVVCGPSPA DLSPGASSVT
PPAPAREPGH SPQ.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpecs 产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-4-1BBR Human, Sf9

ProSpec-4-1BBR Human Sf9,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,4-1BB 受体有助于 T 细胞的克隆扩增、存活和发育。4-1BBR 还可诱导外周单核细胞增殖，增强 TCR/CD3 触发激活诱导的 T 细胞凋亡，并调节 CD28 共刺激促进 Th1 细胞 回应。

4-1BBR Human, Sf9

4-1BB Receptor Human Recombinant, Sf9
CYT-931

Catalogue number

CYT-931

Synonyms

Tumor Necrosis Factor Receptor Superfamily, Member 9, T-Cell Antigen 4-1BB Homolog, 4-1BB Ligand Receptor, T-Cell Antigen ILA, CD137 Antigen, CDw137, CD137, ILA, Interleukin-Activated Receptor, Homolog Of Mouse Ly63, Induced By Lymphocyte Activation , Homolog Of Mouse 4-1BB, Receptor Protein 4-1BB, T Cell Antigen ILA, 4-1BB, Tumor necrosis factor receptor superfamily member 9.
肿瘤坏死因子受体超家族，成员 9，T 细胞抗原 4-1BB 同源物，4-1BB 配体受体，T 细胞抗原 ILA，CD137 抗原，CDw137，CD137，ILA，白介素激活受体，小鼠 Ly63 同源物，诱导 通过淋巴细胞激活，小鼠 4-1BB 同源物，受体蛋白 4-1BB，T 细胞抗原 ILA，4-1BB，肿瘤坏死因子受体超家族成员 9。

Introduction

4-1BBR is a member of the TNF-receptor superfamily.

4-1BB receptor contributes to the clonal expansion, survival, and development of T cells.4-1BBRcan also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
4-1BB 受体有助于 T 细胞的克隆扩增、存活和发育。4-1BBR 还可诱导外周单核细胞增殖，增强 TCR/CD3 触发激活诱导的 T 细胞凋亡，并调节 CD28 共刺激促进 Th1 细胞 回应。 这种受体的表达是由淋巴细胞激活诱导的。 已显示 TRAF 衔接蛋白与该受体结合并转导导致 NF-kappaB 激活的信号。

Description

4-1BBR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 411 amino acids and having a molecular mass of 45.3kDa.

在 Sf9 杆状病毒细胞中产生的 4-1BBR 是单条糖基化多肽链，含有 411 个氨基酸，分子量为 45.3kDa。

4-1BBR is expressed with a 242 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

4-1BBR protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE

Amino acid sequence

ADLFERTRSL QDPCSNCPAG TFCDNNRNQI CSPCPPNSFS SAGGQRTCDI CRQCKGVFRT RKECSSTSNA ECDCTPGFHC LGAGCSMCEQ DCKQGQELTK KGCKDCCFGT FNDQKRGICR PWTNCSLDGK SVLVNGTKER DVVCGPSPAD LSPGASSVTP PAPAREPGHS PQLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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  • 4 1BBL Human, His

ProSpec-4-1BBR Mouse

ProSpec 4-1BBR Mouse,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,该基因编码的蛋白质是 TNF 受体超家族的成员。 该受体有助于 T 细胞的克隆扩增、存活和发育。 它还可以诱导外周单核细胞增殖，增强 TCR/CD3 触发激活诱导的 T 细胞凋亡，并调节 CD28 共刺激以促进 Th1 细胞反应。

4-1BBR Mouse

4-1BB Receptor Mouse Recombinant
CYT-916

ProSpec-4-1BBR Mouse 1µg
ProSpec-4-1BBR Mouse 5µg
ProSpec-4-1BBR Mouse 50µg

Catalogue number

CYT-916

Synonyms

Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, T-cell antigen 4-1BB, CD137.
肿瘤坏死因子受体超家族成员9、4-1BB配体受体、T细胞抗原4-1BB、CD137。

Introduction

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
该基因编码的蛋白质是 TNF 受体超家族的成员。 该受体有助于 T 细胞的克隆扩增、存活和发育。 它还可以诱导外周单核细胞增殖，增强 TCR/CD3 触发激活诱导的 T 细胞凋亡，并调节 CD28 共刺激以促进 Th1 细胞反应。 这种受体的表达是由淋巴细胞激活诱导的。 已显示 TRAF 衔接蛋白与该受体结合并转导导致 NF-kappaB 激活的信号。

Description

4-1BBR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 172 amino acids and having a molecular mass of 18.6kDa .
4-1BBR is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 4-1BBR 是单条糖基化多肽链，含有 172 个氨基酸，分子量为 18.6kDa。
4-1BBR 在 C 末端用 8 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.Sf9，杆状病毒细胞。

Physical Appearance

Sterile Filtered colorless solution.无菌过滤无色溶液。

Formulation

4-1BBR protein solution contains Phosphate Buffered Saline and 10% glycerol.
4-1BBR 蛋白质溶液含有磷酸盐缓冲液和 10% 甘油。

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。

对于长期储存，建议添加载体蛋白。

避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.
大于 90.0%，由-PAGE 确定。

Amino acid sequence

VQNSCDNCQP GTFCRKYNPV CKSCPPSTFS SIGGQPNCNI CRVCAGYFRF KKFCSSTHNA ECECIEGFHC LGPQCTRCEK DCRPGQELTK QGCKTCSLGT FNDQNGTGVC RPWTNCSLDG RSVLKTGTTE KDVVCGPPVV SFSPSTTISV TPEGGPGGHS LQVLLEHHHH HH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 (108-244) Human

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ProSpec,Acrp30 (108-244) Human,ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Acrp30 Human

Adiponectin Human Recombinant
CYT-073

Catalogue number

CYT-073

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Adiponectin is reduced in obese humans, and decreased level is associated with insulin resistance and hyperinsulinemia.
脂联素是一种在血浆中循环的脂肪细胞特异性分泌蛋白。 它在脂肪细胞分化过程中被诱导，其分泌受到胰岛素的刺激。 小鼠脂联素与该人共享约 83% 的氨基酸同一性。 脂联素在各种生理过程中发挥作用，例如能量稳态和肥胖。 肥胖人群中的脂联素降低，而降低的水平与胰岛素抵抗和高胰岛素血症有关。

Description

Acrp30 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18.1kDa.
Acrp30 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 Acrp30 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 158 个氨基酸，分子量为 18.1kDa。
Acrp30 在 N 末端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Formulation

Acrp30 protein solution contains 20mM Tris-HCl buffer , 10% glycerol and 0.4M Urea.
Acrp30 蛋白溶液含有 20mM Tris-HCl 缓冲液、10% 甘油和 0.4M 尿素。

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 80.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAYVYRSAFS VGLETYVTIP NMPIRFTKIF YNQQNHYDGS TGKFHCNIPG LYYFAYHITV YMKDVKVSLF KKDKAMLFTY DQYQENNVDQ ASGSVLLHLE VGDQVWLQVY GEGERNGLYA DNDNDSTFTG FLLYHDTN.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Human

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ProSpec-Acrp30 Human,脂联素，也称为Acrp30、AdipoQ和GBP-28，是最近发现的一种226个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。

Acrp30 Human

Adiponectin Human
CYT-024

Catalogue number

CYT-024

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 226 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

脂联素，也称为Acrp30、AdipoQ和GBP-28，是最近发现的一种226个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Description

The Acrp30 Human protein contains 226 amino acids and having a molecular mass of 24.54 kDa. Native adiponectin is isolated from human pooled sera and contains all HMW, MMW and LMW fractions.

Acrp30 人蛋白含有 226 个氨基酸，分子量为 24.54 kDa。 天然脂联素从人混合血清中分离出来，包含所有 HMW、MMW 和 LMW 级分。

Source

Human pooled serum

Formulation

Acrp30 Human was filtered and lyophilized from 0.5mg/ml supplied in 20mM TRIS, 50mM NaCl and 1mM CaCl2, pH 7.5.

Acrp30 Human 从 20mM TRIS、50mM NaCl 和 1mM CaCl2、pH 7.5 中提供的 0.5mg/ml 过滤和冻干。

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 Acrp30 Human 储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组 Acrp30 Human 可在 4°C 下储存一段有限的时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Human, HEK

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ProSpec-Acrp30 Human HEK,脂联素是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Acrp30 Human, HEK

Adiponectin Human Recombinant, HEK
CYT-434

Catalogue number

CYT-434

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
脂联素是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Description

The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag.
脂联素是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Source

HEK293 .

Biological Activity

In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED50 was ~6 µg/ml.

在原代肝细胞中进行了体外糖异生测定，表明源自哺乳动物细胞的人脂联素可以抑制葡萄糖的产生。 ED50 为~6 µg/ml。

Physical Appearance

White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5mg/ml in 50mM phosphate Buffer, 75mM NaCl, pH 7.4.

Solubility

Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
加入去离子水制备约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请使用合适的无菌过滤器过滤产品。

Stability

For long term, store lyophilized AdipoQ at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable for 24 months when stored at -20°C.
长期保存冻干的 AdipoQ 于 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。 冻干蛋白在 -20°C 下可保持稳定 24 个月。

Purity

Greater than 98% as determined by HPLC and PAGE.

Amino acid sequence

ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Human, His

ProSpec-Acrp30 Human His,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Acrp30 Human 是一种重组蛋白，其 N 端融合了 His 标签。 在大肠杆菌中产生的脂联素 His-Tagged 融合蛋白是 26.4 kDa 蛋白，含有 Acrp30 Human 的 230 个氨基酸残基和 12 个额外的氨基酸残基 – HisTag。

Acrp30 Human, His

Adiponectin Human Recombinant, His tag
CYT-433

Catalogue number

CYT-433

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
脂联素，也称为Acrp30、AdipoQ和GBP-28，是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Description

The Acrp30 Human is created as a recombinant protein with N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the Acrp30 Human and 12 additional amino acid residues – HisTag .
Acrp30 Human 是一种重组蛋白，其 N 端融合了 His 标签。 在大肠杆菌中产生的脂联素 His-Tagged 融合蛋白是 26.4 kDa 蛋白，含有 Acrp30 Human 的 230 个氨基酸残基和 12 个额外的氨基酸残基 – HisTag。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Acrp30 Human was filtered and lyophilized from 0.5 mg/ml in 0.02M Tris buffer pH7.5, 0.15M NaCl.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 Acrp30 Human 储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组 Acrp30 Human 可在 4°C 下储存一段有限的时间； 在 4°C 下两周后它没有显示任何变化。

Amino acid sequence

MRGSHHHHHHGSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY VTIPNMPIRF TKIFYNQQNH YDGSTGKFHC NIPGLYYFAY HITVYMKDVK VSLFKKDKAM LFTYDQYQEN NVDQASGSVL LHLEVGDQVW LQVYGEGERN GLYADNDNDS TFTGFLLYHD TN.

Purity

Acrp30 Human purity is greater than 95% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Human, HMW-CYT-764

ProSpec-Acrp30 Human HMW,ProSpec CYT-764,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,HEK细胞产生的Adiponectin Human Recombinant HMW Rich是一条单一的、糖基化的多肽链，总共包含226个氨基酸，分子量为24.6kDa。 人 Acrp30 HMW Rich 在非还原条件下以 ~ 884 kDa 在 -PAGE 上迁移。

Acrp30 Human, HMW

Adiponectin glycosylated Human Recombinant, HMW Rich
CYT-764

Catalogue number

CYT-764

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
脂联素是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Description

Adiponectin Human Recombinant HMW Rich produced in HEK cells is a single, glycosylated, polypeptide chain containing a total of 226 amino acids, having a molecular mass of 24.6kDa . Human Acrp30 HMW Rich migrates on -PAGE under non-reducing conditions at ~ 884 kDa.

HEK细胞产生的Adiponectin Human Recombinant HMW Rich是一条单一的、糖基化的多肽链，总共包含226个氨基酸，分子量为24.6kDa。 人 Acrp30 HMW Rich 在非还原条件下以 ~ 884 kDa 在 -PAGE 上迁移。

Source

HEK293.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Acrp30 was filtered and lyophilized from 0.5mg/ml in 20mM Tris, 50mM NaCl, pH 7.5 and 1mM CaCl2.

Solubility

It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Acrp30 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入去离子水至工作浓度0.5mg/ml，让冻干颗粒完全溶解。 Acrp30 不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Human, Sf9-CYT-1002

ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ProSpec-Acrp30 Human Sf9,ProSpec CYT-1002,Acrp30 还在造血和免疫系统中充当重要的负调节剂； 它可能通过其抑制功能参与结束炎症反应。 脂联素通过 cAMP 依赖性途径抑制内皮 NF-kappa-b 信号传导，它还抑制 TNF-α 诱导的内皮粘附分子的表达。

Acrp30 Human, Sf9

Adiponectin Human Recombinant, Sf9
CYT-1002

Catalogue number

CYT-1002

Synonyms

Adiponectin, C1Q And Collagen Domain Containing, Adipose Most Abundant Gene Transcript 1 Protein, Adipocyte Complement-Related 30 KDa Protein, 30 KDa Adipocyte Complement-Related Protein, ACRP30, APM-1, GBP28, ACDC, APM1, Adipocyte, C1q And Collagen Domain-Containing Protein, Adipocyte, C1Q And Collagen Domain Containing, Adipose Most Abundant Gene Transcript 1, Adipose Specific Collagen-Like Factor, Gelatin-Binding Protein 28, Gelatin-Binding Protein, Adiponectin Precursor, Adiponectin, ADIPQTL1, ADPN, 30 kDa adipocyte complement-related protein, dipocyte complement-related 30 kDa protein, ACRP30, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein.

含有脂联素、C1Q 和胶原蛋白结构域、脂肪最丰富的基因转录 1 蛋白、脂肪细胞补体相关 30 KDa 蛋白、30 KDa 脂肪细胞补体相关蛋白、ACRP30、APM-1、GBP28、ACDC、APM1、脂肪细胞、C1q 和胶原蛋白结构域 -含有蛋白质、脂肪细胞、C1Q 和胶原蛋白结构域、脂肪最丰富的基因转录物 1、脂肪特异性胶原蛋白样因子、明胶结合蛋白 28、明胶结合蛋白、脂联素前体、脂联素、ADIPQTL1、ADPN、30 kDa 脂肪细胞补体 相关蛋白、双细胞补体相关 30 kDa 蛋白、ACRP30、脂肪细胞、C1q 和含有胶原结构域的蛋白、脂肪最丰富的基因转录物 1 蛋白、apM-1、明胶结合蛋白。

Introduction

The adipose tissue exclusively expresses and secretes Adiponectin . Acrp30 is involved in various physiological processes such as energy homeostasis, insulin sensitivity, hormonal processes, fatty acid metabolism and obesity.
Adiponectin circulates in the plasma. Decreased levels of Adiponectin are associated with insulin resistance and hyperinsulinemia, as seen in people with obesity insulin resistance, and diabetes type 2, whose plasma levels of adiponectin are reduced.
The modular structure of Acrp30 is comprised of N-terminal collagenous domain followed by a C-terminal globular domain.
Acrp30 also acts as a significant negative regulator in hematopoiesis and immune systems; it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin inhibits endothelial NF-kappa-b signaling through a cAMP-dependent pathway, it also inhibits TNF-alpha- induced expression of endothelial adhesion molecules.

脂肪组织专门表达和分泌脂联素。 Acrp30 参与多种生理过程，例如能量稳态、胰岛素敏感性、激素过程、脂肪酸代谢和肥胖。
脂联素在血浆中循环。 脂联素水平降低与胰岛素抵抗和高胰岛素血症有关，如肥胖胰岛素抵抗和 2 型糖尿病患者的血浆脂联素水平降低。
Acrp30 的模块化结构由 N 端胶原结构域和 C 端球状结构域组成。
Acrp30 还在造血和免疫系统中充当重要的负调节剂； 它可能通过其抑制功能参与结束炎症反应。 脂联素通过 cAMP 依赖性途径抑制内皮 NF-kappa-b 信号传导，它还抑制 TNF-α 诱导的内皮粘附分子的表达。

Description

Acrp30 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 146 amino acids and having a molecular mass of 16.9kDa. . Acrp30 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 Acrp30 Human Recombinant 是单条糖基化多肽链，包含 146 个氨基酸，分子量为 16.9kDa。 . Acrp30 在 C 末端用 6 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

Acrp30 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

ADPEGAYVYR SAFSVGLETY VTIPNMPIRF TKIFYNQQNH YDGSTGKFHC NIPGLYYFAY HITVYMKDVK VSLFKKDKAM LFTYDQYQEN NVDQASGSVL LHLEVGDQVW LQVYGEGERN GLYADNDNDS TFTGFLLYHD HHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Human, Trimeric-CYT-233

ProSpec Acrp30 Human Trimeric,ProSpec CYT-233,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,许多研究表明，脂联素具有直接的抗糖尿病、抗动脉粥样硬化和抗炎功能。 APM-1可以增加骨骼肌的胰岛素敏感性。 减弱肝脏脂肪生成和糖异生，调节内皮细胞中 NO 的产生，抑制平滑肌细胞的增殖并防止巨噬细胞的脂质积累。

Acrp30 Human, Trimeric

Adiponectin Human Recombinant, Trimeric form
CYT-233

Catalogue number

CYT-233

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is a hormone exclusively expressed from adipose tissue.
Many studies demonstrate that Adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. APM-1 can increase insulin sensitivity of skeletal muscle. Attenuate hepatic lipogenesis and gluconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells.
In the circulation, Adiponectin is present as three different oligomeric complexes, including the high molecular weight , the middle molecular weight and low molecular weigh forms 8. Different oligomeric complex of Adiponectin activates different signaling pathways and exerts distinct functions.
脂联素是一种仅由脂肪组织表达的激素。
许多研究表明，脂联素具有直接的抗糖尿病、抗动脉粥样硬化和抗炎功能。 APM-1可以增加骨骼肌的胰岛素敏感性。 减弱肝脏脂肪生成和糖异生，调节内皮细胞中 NO 的产生，抑制平滑肌细胞的增殖并防止巨噬细胞的脂质积累。
在循环中，脂联素以三种不同的寡聚体形式存在，包括高分子量、中分子量和低分子量的8种。不同的脂联素寡聚体激活不同的信号通路，发挥不同的功能。

Description

Trimeric form of Adiponectin Human trimeric form was expressed in HEK293 cells. The cysteine 39 was replaced with Alanine 9. hAd-C39A can only form a trimer, but not a hexamer or an HMW form.
脂联素的三聚体形式 人三聚体形式在 HEK293 细胞中表达。 半胱氨酸 39 被丙氨酸 9 取代。hAd-C39A 只能形成三聚体，而不能形成六聚体或 HMW 形式。

Source

脂联素的三聚体形式 人三聚体形式在 HEK293 细胞中表达。 半胱氨酸 39 被丙氨酸 9 取代。hAd-C39A 只能形成三聚体，而不能形成六聚体或 HMW 形式。

HEK293 .

Physical Appearance

Filtered White lyophilized powder.
过滤的白色冻干粉末。

Formulation

Filtered and lyophilized from 0.5mg/ml in 0.05M phosphate buffer, 0.075M NaCl, pH 7.4.

Solubility

Add deionized water to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
加入去离子水至工作体积为 0.5mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized Adiponectin at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的脂联素储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95% as determined by -PAGE.

Biological Activity

ED50= 3-8.5 μg/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF.

Amino acid sequence

ETTTQGPGVL LPLPKGAATG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DDK.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Mouse-CYT-1168

ProSpec Acrp30 Mouse,ProSpec CYT-1168,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,脂联素又名Acrp30、AdipoQ和GBP-28，是一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。

Acrp30 Mouse

Adiponectin Mouse Recombinant
CYT-1168

Catalogue number

CYT-1168

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin, also known as Acrp30, AdipoQ and GBP-28, is a 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . Adiponectin is a part of the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

脂联素又名Acrp30、AdipoQ和GBP-28，是一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 脂联素是可溶性防御胶原蛋白超家族的一部分； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Description

Acrp30 Mouse Recombinant is a single, glycosylated polypeptide chain containing 230 amino acids and having a molecular mass of 24.9kDa

Acrp30 Mouse Recombinant 是单条糖基化多肽链，包含 230 个氨基酸，分子量为 24.9kDa

Source

HEK293 cells.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Acrp30 filtered and lyophilized from 0.5mg/ml in 20mM Tris 50mM NaCl and 1mM CaCl2, pH 7.5.

Acrp30 在 20mM Tris 50mM NaCl 和 1mM CaCl2，pH 7.5 中从 0.5mg/ml 过滤和冻干。

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.

建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 90.0% as determined by -PAGE. 

Biological Activity

Measured by its ability to inhibit proliferation of mouse 3T3 fibroblasts stimulated by PDGF. The ED50 for the effect is typically 5-15 μg/mL.

Amino acid sequence

EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYVYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Mouse, HEK-CYT-435

ProSpec Acrp30 Mouse HEK,ProSpec CYT-435,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,脂联素是造血和免疫系统中重要的负调节剂。 它可能通过其抑制功能参与结束炎症反应。 通过依赖营的途径抑制内皮 nf-kappa-b 信号传导。 抑制 TNF-α 诱导的内皮粘附分子的表达。 参与控制脂肪代谢和胰岛素敏感性。

Acrp30 Mouse, HEK

Adiponectin Mouse Recombinant, HEK
CYT-435

Catalogue number

CYT-435

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is an important negative regulator in hematopoiesis and immune systems. It may be involved in ending inflammatory responses through its inhibitory functions. Inhibits endothelial nf-kappa-b signaling through a camp-dependent pathway. Inhibits tnf-alpha- induced expression of endothelial adhesion molecules. Involved in the control of fat metabolism and insulin sensitivity.
脂联素是造血和免疫系统中重要的负调节剂。 它可能通过其抑制功能参与结束炎症反应。 通过依赖营的途径抑制内皮 nf-kappa-b 信号传导。 抑制 TNF-α 诱导的内皮粘附分子的表达。 参与控制脂肪代谢和胰岛素敏感性。

Description

The Acrp30 Mouse Recombinant is fused with FLAG tag having a total Mw of 26kDa.
Acrp30 Mouse Recombinant 与总 Mw 为 26kDa 的 FLAG 标签融合。

Source

HEK293 .

Formulation

Filtered and lyophilized from 0.5 mg/ml in 0.05M PBS buffer,0.075M NaCl, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized Acrp30 Mouse in sterile 18M-cm H2O at 0.5mg/ml, which can then be further diluted to other aqueous solutions.
建议在无菌 18M-cm H2O 中以 0.5mg/ml 的浓度重建冻干的 Acrp30 小鼠，然后可以将其进一步稀释成其他水溶液。

Stability

Store lyophilized APM-1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 APM-1 储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 98.0% as determined by -PAGE.

Amino acid sequence

EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK.

Biological Activity

Full-length adiponectin activates AMP-activated protein kinase in hepatocyte. Adiponectin also activates AMPK in HepG2 human hepatocytes at the concentration of 1.0 µg/ml.  An in vitro gluconeogenesis assay which was performed in primary rat hepatocytes showed the murine adiponectin derived from mammalian cells can inhibit glucose production.

全长脂联素激活肝细胞中的 AMP 活化蛋白激酶。 浓度为 1.0 µg/ml 的脂联素还能激活 HepG2 人肝细胞中的 AMPK。 在原代大鼠肝细胞中进行的体外糖异生测定表明，源自哺乳动物细胞的鼠脂联素可以抑制葡萄糖的产生。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Mouse, His-CYT-537

ProSpec Acrp30 Mouse His,ProSpec CYT-537,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,脂联素是一种在血浆中循环的脂肪细胞特异性分泌蛋白。 它在脂肪细胞分化过程中被诱导，其分泌受到胰岛素的刺激。

Acrp30 Mouse, His

Adiponectin Mouse Recombinant, His Tag
CYT-537

Catalogue number

CYT-537

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Adiponectin is reduced in obese humans, and decreased level is associated with insulin resistance and hyperinsulinemia.
脂联素是一种在血浆中循环的脂肪细胞特异性分泌蛋白。 它在脂肪细胞分化过程中被诱导，其分泌受到胰岛素的刺激。 小鼠脂联素与该人共享约 83% 的氨基酸同一性。 脂联素在各种生理过程中发挥作用，例如能量稳态和肥胖。 肥胖人群中的脂联素降低，而降低的水平与胰岛素抵抗和高胰岛素血症有关。

Description

The Adiponectin Mouse is created as a recombinant protein with a 21 a.a N-terminal fusion of His Tag. The Adiponectin His-Tagged Fusion Protein, produced in E. coli, is a 27.2kDa protein containing 251 amino acid residues of the Acrp30 Mouse, 18-247 amino acids.
脂联素小鼠是一种重组蛋白，具有 21 a.a N 端的 His 标签融合。 在大肠杆菌中产生的脂联素 His-Tagged 融合蛋白是一种 27.2kDa 的蛋白质，含有 Acrp30 小鼠的 251 个氨基酸残基，18-247 个氨基酸。

Source

Escherichia Coli.

Formulation

Acrp30 Mouse is a sterile filtered liquid formulation containing 20mM Tris-HCl pH-8, 1mM DTT and 10% Glycerol.
Acrp30 Mouse 是一种无菌过滤液体制剂，含有 20mM Tris-HCl pH-8、1mM DTT 和 10% 甘油。

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Acrp30 Mouse purity is greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MEDDVTTTEE LAPALVPPPK GTCAGWMAGI PGHPGHNGTP GRDGRDGTPG EKGEKGDAGL LGPKGETGDV GMTGAEGPRG FPGTPGRKGE PGEAAYVYRS AFSVGLETRV TVPNVPIRFT KIFYNQQNHY DGSTGKFYCN IPGLYYFSYH ITVYMKDVKV SLFKKDKAVL FTYDQYQEKN VDQASGSVLL HLEVGDQVWL QVYGDGDHNG LYADNVNDST FTGFLLYHDT N.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Mouse, Trimeric/CYT-247

ProSpec Acrp30 Mouse Trimeric,ProSpec CYT-247,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,加入去离子水，制备约 0.5 mg/mL 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Acrp30 Mouse, Trimeric

Adiponectin Mouse Recombinant, Trimeric form
CYT-247

Catalogue number

CYT-247

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is a hormone exclusively expressed from adipose tissue. Many studies demonstrate that adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. APM-1 can increase insulin sensitivity of skeletal muscle, attenuate hepatic lipogenesis and luconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells.
In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight , the middle molecular weight and low molecular weigh forms 8. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.
脂联素是一种仅由脂肪组织表达的激素。 许多研究表明，脂联素具有直接的抗糖尿病、抗动脉粥样硬化和抗炎功能。 APM-1 可以增加骨骼肌的胰岛素敏感性，减弱肝脏脂肪生成和糖异生，调节内皮细胞中 NO 的产生，抑制平滑肌细胞的增殖，防止巨噬细胞的脂质积累。
在循环中，脂联素以三种不同的寡聚体形式存在，包括高分子量、中分子量和低分子量的8种。不同的脂联素寡聚体激活不同的信号通路，发挥不同的功能。

Description

Trimeric form of Acrp30 Mouse was expressed in HEK293 cells.
The cysteine 39 was replaced with alanine 9. mAd-C39A can only form trimer, but not hexamer or HMW form.
Acrp30 小鼠的三聚体形式在 HEK293 细胞中表达。
半胱氨酸39被丙氨酸9取代。mAd-C39A只能形成三聚体，不能形成六聚体或HMW。

Source

HEK293 .

Physical Appearance

Filtered white lyophilized powder.

Formulation

Mouse Acrp30 filtered and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer, 0.05M NaCl, pH 7.2.
在 0.05M 磷酸盐缓冲液、0.05M NaCl、pH 7.2 中过滤和冻干小鼠 Acrp30，浓度为 0.5 mg/ml。

Solubility

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
加入去离子水，制备约 0.5 mg/mL 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized Acrp30 Mouse at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Mouse can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 Acrp30 小鼠储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组的 Acrp30 小鼠可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Acrp30 Mouse purity is greater than 95% as determined by -PAGE.

References

Title: Evidence that adiponectin receptor 1 activation exacerbates ischemic neuronal death
Publication: Experimental & translational stroke medicine 2.1 : 15.
Link: http://www.etsmjournal.com/content/2/1/15

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-Acrp30 Protein/CYT-280

ProSpec Acrp30 Protein,ProSpec CYT-280,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Acrp30 还在造血和免疫系统中充当重要的负调节剂； 它可能通过其抑制功能参与结束炎症反应。 脂联素通过 cAMP 依赖性途径抑制内皮 NF-kappa-b 信号传导，它还抑制 TNF-α 诱导的内皮粘附分子的表达。

Acrp30 Protein

Adiponectin Human Recombinant
CYT-280

Catalogue number

CYT-280

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

The adipose tissue exclusively expresses and secretes Adiponectin . Acrp30 is involved in various physiological processes such as energy homeostasis, insulin sensitivity, hormonal processes, fatty acid metabolism and obesity.
Adiponectin circulates in the plasma. Decreased levels of Adiponectin are associated with insulin resistance and hyperinsulinemia, as seen in people with obesity insulin resistance, and diabetes type 2, whose plasma levels of adiponectin are reduced.
The modular structure of Acrp30 is comprised of N-terminal collagenous domain followed by a C-terminal globular domain.
Acrp30 also acts as a significant negative regulator in hematopoiesis and immune systems; it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin inhibits endothelial NF-kappa-b signaling through a cAMP-dependent pathway, it also inhibits TNF-alpha- induced expression of endothelial adhesion molecules.
脂肪组织专门表达和分泌脂联素。 Acrp30 参与多种生理过程，例如能量稳态、胰岛素敏感性、激素过程、脂肪酸代谢和肥胖。
脂联素在血浆中循环。 脂联素水平降低与胰岛素抵抗和高胰岛素血症有关，如肥胖胰岛素抵抗和 2 型糖尿病患者的血浆脂联素水平降低。
Acrp30 的模块化结构由 N 端胶原结构域和 C 端球状结构域组成。
Acrp30 还在造血和免疫系统中充当重要的负调节剂； 它可能通过其抑制功能参与结束炎症反应。 脂联素通过 cAMP 依赖性途径抑制内皮 NF-kappa-b 信号传导，它还抑制 TNF-α 诱导的内皮粘附分子的表达。

Description

The Adiponectin Human recombinant protein is a single, non-glycosilated polypeptide chain produced in E. coli, having a molecular weight of 25.1 kDa and containing 231 amino acids .
脂联素人重组蛋白是在大肠杆菌中产生的单一非糖基化多肽链，分子量为 25.1 kDa，含有 231 个氨基酸。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

Acrp30 protein solution contains Phosphate buffered saline pH 7.4 and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Acrp30 purity is greater than 90% as determined by -PAGE.

Amino acid sequence

MGHDQETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGE
KGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSV
GLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKD
VKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGE
RNGLYADNDNDSTFTGFLLYHDTN.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-ACRP30 Rat/CYT-831

ACRP30 Rat

Adiponectin Rat Recombinant

CYT-831

ProSpec-ACRP30 Rat 2µg

Catalogue number

CYT-831

Synonyms

Adipocyte C1q and collagen domain-containing protein, Adipocyte complement-related 30kDa protein, Adipose most abundant gene transcript 1 protein, gelatin-binding protein, Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
脂肪细胞 C1q 和胶原蛋白结构域蛋白、脂肪细胞补体相关 30kDa 蛋白、脂肪最丰富的基因转录物 1 蛋白、明胶结合蛋白、Acrp30、AdipoQ、GBP-28、APM-1、ACDC。

Introduction

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells . The protein belongs to the soluble defense collagen super family; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.
脂联素，也称为Acrp30、AdipoQ和GBP-28，是最近发现的一种244个氨基酸的蛋白质，是apM1基因的产物，具有生理活性，在脂肪细胞中特异性高表达。 该蛋白质属于可溶性防御胶原蛋白超家族； 它具有与胶原 VIII 和 X 结构同源的胶原样结构域和补体因子 C1q 样球状结构域。 APM-1 形成同源三聚体，它们是血清中循环的高阶复合物的组成部分。

Description

ACRP30 Rat Recombinant produced in 293 cell line is a polypeptide chain containing 238 amino acids and having a total molecular mass of 25.7 kDa. ACRP30 contains a C-Terminal linker and a C-Terminal Flag- tag .
293细胞系产生的ACRP30 Rat Recombinant是一条含有238个氨基酸的多肽链，总分子量为25.7 kDa。 ACRP30 包含一个 C-Terminal 链接器和一个 C-Terminal Flag-tag。

Source

HEK293 cell line.

Physical Appearance

White lyophilized powder.

Formulation

Filtered and lyophilized in 20 mM Tris buffer, 50 mM NaCl, pH 7.5.

Solubility

Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
加入去离子水至工作浓度约为 0.5 mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Lyophilized ACRP30 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ACRP30 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 ACRP30 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，ACRP30 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

GITATEGPGA LVPPPKETCA GWMAGIPGYP GHNGIPGRDG RDGTPGEKGE KGDAGVLGPK GDPGDAGMTG AEGPRGFPGT PGRKGEPGEA AYMYHSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFHCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSMLLHLEV GDQVWLQVYG EGDNNGLYAD NVNDSTFTGF LLYHDTNAAA DYKDDDDK

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-gAcrp30 Human/CYT-615

ProSpec gAcrp30 Human,ProSpec CYT-615,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,gAcrp30 球状蛋白是脂联素蛋白水解加工的结果。 脂联素仅由脂肪细胞制造和分泌，是一种高度获得的血浆蛋白，占血清总蛋白的0.05%。 与脂联素类似，gAcrp30 能够降低高血糖并逆转 INS 抗性。

gAcrp30 Human

Globular Adiponectin Human Recombinant
CYT-615

Catalogue number

CYT-615

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

gAcrp30 globular protein, exists as a result of proteolytic processing of adiponectin. Adiponectin is manufactured and secreted solely by adipocytes, and is a highly obtained plasma protein, accounting for up to 0.05% of total serum protein. Similar to Adiponectin, gAcrp30 is able of lowering hyperglycemia and reversing INS resistance. In addition, gAcrp30 is an significant protein that is involved in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids. AdipoR1 & AdipoR2 are the 2 signaling receptors for adiponectin and gAcrp30 that were recently been identified.

gAcrp30 球状蛋白是脂联素蛋白水解加工的结果。 脂联素仅由脂肪细胞制造和分泌，是一种高度获得的血浆蛋白，占血清总蛋白的0.05%。 与脂联素类似，gAcrp30 能够降低高血糖并逆转 INS 抗性。 此外，gAcrp30 是一种重要的蛋白质，它通过向肌肉发出信号以吸收和燃烧游离脂肪酸来促进脂肪减少。 AdipoR1 和 AdipoR2 是最近发现的脂联素和 gAcrp30 的 2 个信号受体。

Description

gAcrp30 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 145 amino acids and having a molecular mass of 16.7kDa.
The gAcrp30 is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 gAcrp30 Human Recombinant 是一种非糖基化的多肽链，含有 145 个氨基酸，分子量为 16.7kDa。
gAcrp30 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Formulation

 

Solubility

We recommended reconstituting gAcrp30 at a concentration of 0.1mg per ml with 10mM sodium phosphate & 0.5mM DTT, pH 7.5. which can then be further diluted to other aqueous solutions.

我们建议使用 10mM 磷酸钠和 0.5mM DTT，pH 7.5 以 0.1mg/ml 的浓度重构 gAcrp30。 然后可以进一步稀释成其他水溶液。

Stability

For long term, store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
Reconstituted protein can be stored at 4°C for a limited period of time two weeks.
长期保存冻干蛋白在-20°C。 重组后分装产品以避免重复的冷冻/解冻循环。
重构的蛋白质可以在 4°C 下储存两周的有限时间。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN.

Biological Activity

gAcrp30 activity is determined by its ability to inhibit the proliferation of mouse M1 cells. The expected ED50 for this effect is 669ng/ml, corresponding to a specific activity of 1.5×103units/mg.

gAcrp30 的活性取决于其抑制小鼠 M1 细胞增殖的能力。 这种效果的预期 ED50 为 669ng/ml，对应于 1.5x103units/mg 的比活性。

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-gAcrp30 Human, His/CYT-277

 

  • gAcrp30 Human, His

  • Globular Adiponectin Human Recombinant, His Tag
  • CYT-277
  • 2µg

Catalogue number

CYT-277

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is a protein exclusively secreted from adipose tissue. In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight , the middle molecular weight and low molecular weigh forms. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.
脂联素是一种专门从脂肪组织分泌的蛋白质。 在循环中，脂联素以三种不同的低聚复合物存在，包括高分子量、中分子量和低分子量形式。 不同的脂联素寡聚复合物激活不同的信号通路并发挥不同的功能。

Description

Acrp30 Human has a total of 171 amino acids. N-terminal underlined amino acids are His-tag and the protease cleavage site . The AA sequence of Acrp30 Human is homologous to the 105-244 amino acid sequence of the Human full-length Adiponectin .
Acrp30 Human 共有 171 个氨基酸。 N端带下划线的氨基酸是His标签和蛋白酶切割位点。 Acrp30 Human 的 AA 序列与 Human 全长脂联素的 105-244 个氨基酸序列同源。

Source

Escherichia Coli.

Formulation

Acrp30 Human is a filtered powder, lyophilized from 0.6mg/ml in PBS buffer.

Solubility

Add deionized water and let the lyophilized pellet of Acrp30 Human dissolve completely.

Stability

For long term, store lyophilized Acrp30 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized Acrp30 Human remains stable for 24 months when stored at -20°C.
长期保存冻干的 Acrp30 Human 于 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组 Acrp30 Human 可在 4°C 下储存一段有限的时间； 在 4°C 下两周后它没有显示任何变化。 冻干的 Acrp30 Human 可在 -20°C 下保存 24 个月。

Purity

Purity of Acrp30 Human is greater than 95% as determined by PAGE.

Amino acid sequence

MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEGAYVYRS FSVGLETYV TIPNMPIRFT KIFYNQQNHYDGSTGKFHCN IPGLYYFAYH ITVYMKDVKV SLFKKDKAML FTYDQYQENN VDQASGSVLL HEVGDQVWLQVYGEGERNGL YADNDNDSTF TGFLLYHDTN.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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  • Acrp30 Mouse, HEK
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ProSpec-gAcrp30 Mouse/CYT-432

ProSpec gAcrp30 Mouse,ProSpec CYT-432,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Adiponectin Mouse Recombinant / Acrp30 Mouse 的球状结构域包含来自 a.a. 的 138 个氨基酸残基。 具有 16 kDa 分子量的 111-247 在大肠杆菌中过表达，并通过使用常规色谱技术进行纯化。

 

gAcrp30 Mouse

  • Globular Adiponectin Mouse Recombinant
  • CYT-432

Catalogue number

CYT-432

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Introduction

Adiponectin is adipocyte complement-related protein of 30 kDa and exclusively expressed in differentiated adipocytes. APM-1 is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and domain .
Adiponectin expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 Mouse or gAcrp30 lowers serum glucose and free fatty acid level in mice.
脂联素是 30 kDa 的脂肪细胞补体相关蛋白，仅在分化的脂肪细胞中表达。 APM-1是补体因子C1q家族的成员，由信号序列、非同源序列、胶原结构域和结构域组成。
在人类、猴子和小鼠的各种肥胖和胰岛素抵抗状态下，脂联素的表达降低。 注射 Acrp30 Mouse 或 gAcrp30 可降低小鼠的血清葡萄糖和游离脂肪酸水平。

Description

The globular domain of Adiponectin Mouse Recombinant / Acrp30 Mouse contains 138 amino acid residues from a.a. 111-247 having molecular mass of 16 kDa was over expressed in E.coli and purified by using conventional chromatography techniques.
Adiponectin Mouse Recombinant / Acrp30 Mouse 的球状结构域包含来自 a.a. 的 138 个氨基酸残基。 具有 16 kDa 分子量的 111-247 在大肠杆菌中过表达，并通过使用常规色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

Acrp30 Mouse solution containing 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT and 10% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITVYMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYADNVNDSTFTG FLLYHDTN

References

Title: Evidence that adiponectin receptor 1 activation exacerbates ischemic neuronal death
Publication: Experimental & translational stroke medicine 2.1 : 15.
Link: http://www.etsmjournal.com/content/2/1/15

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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  • Acrp30 Human, Sf9

ProSpec-AITR Human/CYT-925

ProSpec AITR Human,ProSpec CYT-925,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,肿瘤坏死因子受体超家族成员18是肿瘤坏死因子受体超家族的成员。 AITR 是 TNFSF18 的受体，似乎参与活化的 T 淋巴细胞和内皮细胞之间的相互作用以及 T 细胞受体介导的细胞死亡的调节。 AITR 通过 TRAF2/NIK 通路介导 NF-kappa-B 激活。

AITR Human

AITR Human Recombinant
CYT-925

Catalogue number

CYT-925

Synonyms

TNFRSF18, AITR, CD357, GITR, GITR-D, Tumor necrosis factor receptor superfamily member 18, Activation-inducible TNFR family receptor, Glucocorticoid-induced TNFR-related protein, CD357, UNQ319/PRO364.

Introduction

Tumor necrosis factor receptor superfamily member 18 is a member of the tumor necrosis factor receptor superfamily. AITR is receptor for TNFSF18 and appears to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. AITR is mediates NF-kappa-B activation via the TRAF2/NIK pathway.
肿瘤坏死因子受体超家族成员18是肿瘤坏死因子受体超家族的成员。 AITR 是 TNFSF18 的受体，似乎参与活化的 T 淋巴细胞和内皮细胞之间的相互作用以及 T 细胞受体介导的细胞死亡的调节。 AITR 通过 TRAF2/NIK 通路介导 NF-kappa-B 激活。

Description

AITR Human Recombinant produced in Sf9 Baculovirus is a single, glycosylated polypeptide chain containing 145 amino acids and having a molecular mass of 15.6kDa .
AITR is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒中产生的 AITR 人重组体是一条单一的糖基化多肽链，包含 145 个氨基酸，分子量为 15.6kDa。
AITR 在 C 末端与 8 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

AITR protein solution containing Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

QRPTGGPGCG PGRLLLGTGT DARCCRVHTT RCCRDYPGEE CCSEWDCMCV QPEFHCGDPC CTTCRHHPCP PGQGVQSQGK FSFGFQCIDC ASGTFSGGHE GHCKPWTDCT QFGFLTVFPG NKTHNAVCVP GSPPAEPLEH HHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-AITRL Human, T7/CYT-807

ProSpec AITRL Human T7,ProSpec CYT-807,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Osteostat 不抑制单核细胞和树突细胞中脂多糖诱导的 RANK 表达，或 T 细胞中活化诱导的 RANK 表达。 Osteostat 是一种新的破骨细胞生成调节剂，证实了内皮在骨生理学中的主要作用。

AITRL Human, T7
AITRL Human Recombinant, T7 Tag
CYT-807
ProSpec-AITRL Human, T7/CYT-807 2µg
ProSpec-AITRL Human, T7/CYT-807 10µg
ProSpec-AITRL Human, T7/CYT-8071mg

Catalogue number

CYT-807

Synonyms

Osteostat, TNFSF18, Activation-induced TNFR member Ligand, GITRL,TL6, AITRL, Glucocorticoid-induced TNF-related ligand, hGITRL, Tumor necrosis factor ligand superfamily member 18, MGC138237.

Introduction

Osteostat is the cytokine that binds to TNFRSF18/AITR/GITR and is important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. Osteostat is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells after stimulation by lipopolysaccharides.
Osteostat protein is detectable in human microvascular EC and is highly up-regulated by IFN-alpha and IFN-beta. Osteostat inhibit differentiation of osteoclasts from monocytic precursor cells. Osteostat suppresses the early stage of osteoclastogenesis via inhibition of macrophage colony-stimulating factorinduced receptor activator of NF-kappaB expression in the osteoclast precursor cells. Osteostat does not inhibit lipopolysaccharide-induced RANK expression in monocytes and dendritic cells, or activation-induced RANK expression in T cells. Osteostat is a novel regulator of osteoclast generation and substantiate the major role played by the endothelium in bone physiology.
Osteostat 是与 TNFRSF18/AITR/GITR 结合的细胞因子，对活化的 T 淋巴细胞和内皮细胞之间的相互作用很重要，并可调节外周组织中的 T 淋巴细胞存活。在脂多糖刺激后，Osteostat 在小肠、卵巢、睾丸、肾和内皮细胞中高水平表达。
Osteostat 蛋白可在人微血管 EC 中检测到，并且被 IFN-α 和 IFN-β 高度上调。 Osteostat 抑制破骨细胞从单核细胞前体细胞分化。 Osteostat 通过抑制巨噬细胞集落刺激因子诱导的破骨细胞前体细胞中 NF-kappaB 表达的受体激活剂来抑制破骨细胞生成的早期阶段。 Osteostat 不抑制单核细胞和树突细胞中脂多糖诱导的 RANK 表达，或 T 细胞中活化诱导的 RANK 表达。 Osteostat 是一种新的破骨细胞生成调节剂，证实了内皮在骨生理学中的主要作用。

Description

AITRL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15kDa.
AITRL is fused to a 15 amino acid T7-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 AITRL Human Recombinant 是一条单一的、非糖基化的多肽链，包含 134 个氨基酸，分子量为 15kDa。
AITRL 在 N 端与 15 个氨基酸的 T7 标签融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

AITRL protein solution contains 20mM Tris-HCl buffer , 10% glycerol and 0.4M Urea.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MASMTGGQQM GRGSHMAKFG PLPSKWQMAS SEPPCVNKVS DWKLEILQNG LYLIYGQVAP NANYNDVAPF EVRLYKNKDM IQTLTNKSKI QNVGGTYELH VGDTIDLIFN SEHQVLKNNT YWGIILLANP QFIS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-AIF1 Human/CYT-697

ProSpec AIF1 Human,ProSpec CYT-697,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,AIF1 Human Recombinant 共含有 155 个氨基酸，分子量为 17.7kDa。 人类 AIF1 融合到一个 9 个氨基酸长的 N 端 His 标签。

AIF1 Human
Allograft Inflammatory Factor 1 Human Recombinant
CYT-697

ProSpec-AIF1 Human/CYT-697 2µg
ProSpec-AIF1 Human/CYT-697 10µg
ProSpec-AIF1 Human/CYT-697 1mg

Catalogue number

CYT-697

Synonyms

AIF-1, Allograft inflammatory factor 1, Em:AF129756.17, G1, IBA1, Ionized calcium-binding adapter molecule 1, IRT-1, Protein G1, AIF1.

Introduction

Human AIF1 protein shares 98% homology/identity with that of rat. AIF1 is expressed in macrophages and neutrophils. The expression of AIF1 transcripts is upregulated by IFN-g in rat macrophages. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68 macrophages in the interstitial and perivascular spaces of human heart allografts. In quiescent cultured human vascular smooth muscle cells synthesis of AIF1 is induced by IFN-g, IL1b, and conditioned medium of T-cells. Overexpression of AIF1 in human VSMCs results in enhanced growth of these cells. AIF1 is expressed during apoptosis rat mammary gland and ventral prostate tissues. Allograft Inflammatory Factor 1 is expressed by several tumor-associated activated macrophages and microglial cells in rat and human gliomas. There is an evident relationship of AIF1-expressing activated macrophages and microglial cells with tumor malignancy in humans.
人类 AIF1 蛋白与大鼠具有 98% 的同源性/同一性。 AIF1 在巨噬细胞和中性粒细胞中表达。 AIF1 转录物的表达在大鼠巨噬细胞中被 IFN-g 上调。 AIF1 在人类巨噬细胞样细胞系和人类心脏同种异体移植物间质和血管周围空间的 CD68 巨噬细胞亚群中选择性表达。 在静止培养的人血管平滑肌细胞中，AIF1 的合成由 IFN-g、IL1b 和 T 细胞条件培养基诱导。 AIF1 在人类 VSMC 中的过表达导致这些细胞的生长增强。 AIF1 在大鼠乳腺和腹侧前列腺组织细胞凋亡期间表达。 同种异体移植炎症因子 1 由大鼠和人类神经胶质瘤中的几种肿瘤相关活化巨噬细胞和小胶质细胞表达。 表达 AIF1 的活化巨噬细胞和小胶质细胞与人类肿瘤恶性肿瘤有明显的关系。

Description

The AIF1 Human Recombinant contains a total of 155 amino acids having a molecular Mass of 17.7kDa. The Human AIF1 is fused to a 9 amino acid long N-terminal His tag.
AIF1 Human Recombinant 共含有 155 个氨基酸，分子量为 17.7kDa。 人类 AIF1 融合到一个 9 个氨基酸长的 N 端 His 标签。

Source

E. Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.

Solubility

Add deionized water and let the lyophilized pellet dissolve completely.

Stability

For long term, store lyophilized AIF1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable for 24 months when stored at -20°C.
长期保存冻干的 AIF1 在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。
冻干蛋白在 -20°C 下可保持稳定 24 个月。

Amino acid sequence

MKHHHHHHASQTRDLQGGKAF­GLLKAQQEERLDE­INKQFLDDPKYSSDED­LPSKLEGFKEKYMEF­DLNGNGDIDIMSLKRMLEK­LGVPKTHLELKKLI­GEVSSGSGETFSYP­DFLRMMLGKRSAIL­KMILMYEEKAREKEK­PTGPPAKKAISELP.

Purity

Greater than 90% as determined by PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-AIF1L Human/CYT-007

ProSpec AIF1L Human,ProSpec CYT-007,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,AIF1L 是一种细胞质、细胞因子诱导的钙结合蛋白，在具有慢性排斥反应的大鼠心脏同种异体移植物中发现。 AIF1L 蛋白是人类小胶质细胞的标志物，由受损骨骼肌中的巨噬细胞表达，并调节卫星细胞的增殖和分化减少。

AIF1L Human

Allograft Inflammatory Factor 1 Like Human Recombinant
CYT-007
ProSpec-AIF1L Human/CYT-007 5µg
ProSpec-AIF1L Human/CYT-00720µg
ProSpec-AIF1L Human/CYT-007 1mg

Catalogue number

CYT-007

Synonyms

Allograft Inflammatory Factor 1-like, IBA2, FLJ12783, Ionized calcium-binding adapter molecule 2, C9orf58, chromosome 9 open reading frame 58, MGC29466.
同种异体炎症因子 1 样，IBA2，FLJ12783，离子钙结合衔接分子 2，C9orf58，染色体 9 开放阅读框 58，MGC29466。

Introduction

AIF1L is a cytoplasmic, cytokine-induced, calcium-binding protein which was discovered in rat cardiac allografts with chronic rejection. AIF1L protein is a marker of human microglia and is expressed by macrophages in injured skeletal muscle and regulates reduced proliferation and differentiation of satellite cells.
AIF1L 是一种细胞质、细胞因子诱导的钙结合蛋白，在具有慢性排斥反应的大鼠心脏同种异体移植物中发现。 AIF1L 蛋白是人类小胶质细胞的标志物，由受损骨骼肌中的巨噬细胞表达，并调节卫星细胞的增殖和分化减少。

Description

AIF1L produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids and having a molecular mass of 19.2kDa.
AIF1L is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The AIF1L protein solution is formulated in 20mM Tris-HCl buffer , 1mM DTT, 0.1mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD LNNEGEIDLM SLKRMMEKLG VPKTHLEMKK MISEVTGGVS DTISYRDFVN MMLGKRSAVL KLVMMFEGKA NESSPKPVGP PPERDIASLP.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpecs 产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-ANGPT1 Human/CYT-074

ProSpec ANGPT1 Human,ProSpec CYT-074,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Angiopoietin-1 的胚胎表达模式表明它在发育中的心脏中起着特别重要的作用。血管生成素-1 在表达 TIE-2 的心内膜周围的心肌壁中高度表达。 Angiopoietin-1 的表达在发育后期变得更加普遍。

ANGPT1 Human

Angiopoietin-1 Human Recombinant
CYT-074
ProSpec-ANGPT1 Human/CYT-074 5µg
ProSpec-ANGPT1 Human/CYT-074 20µg
ProSpec-ANGPT1 Human/CYT-074 1mg

Catalogue number

CYT-074

Synonyms

Angiopoietin 1, KIAA0003, ANG-1, AGP1, AGPT.

Introduction

ANGPT1 is an angiogenic factor which intervenes in blood vessel maturation and takes part in endothelial development. ANGPT1 is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. The glycosylated ANGPT1 protein has a coiled-coil region in the amino terminus and a fibrinogen-like domain at the carboxy terminus.
ANGPT1是一种血管生成因子，可干预血管成熟并参与内皮发育。 ANGPT1 是 Tie-2 的分泌配体，Tie-2 是一种在内皮细胞和造血细胞中表达的细胞表面受体酪氨酸激酶。 糖基化的 ANGPT1 蛋白在氨基末端有一个卷曲螺旋区，在羧基末端有一个纤维蛋白原样结构域。

Description

ANGPT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 480 amino acids and having a molecular mass of 55.6 kDa.
The ANGPT1 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 ANGPT1 Human Recombinant 是单条非糖基化多肽链，包含 480 个氨基酸，分子量为 55.6 kDa。
ANGPT1 通过专有的色谱技术进行纯化。

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The ANGPT1 solution contains 20mM Tris-HCl buffer , 1M Urea and 5% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85% as determined by -PAGE.

Amino acid sequence

MSNQRRSPEN SGRRYNRIQH GQCAYTFILP EHDGNCREST TDQYNTNALQ RDAPHVEPDF SSQKLQHLEH VMENYTQWLQ KLENYIVENM KSEMAQIQQN AVQNHTATML EIGTSLLSQT AEQTRKLTDV ETQVLNQTSR LEIQLLENSL STYKLEKQLL QQTNEILKIH EKNSLLEHKI LEMEGKHKEE LDTLKEEKEN LQGLVTRQTY IIQELEKQLN RATTNNSVLQ KQQLELMDTV HNLVNLCTKE GVLLKGGKRE EEKPFRDCAD VYQAGFNKSG IYTIYINNMP EPKKVFCNMD VNGGGWTVIQ HREDGSLDFQ RGWKEYKMGF GNPSGEYWLG NEFIFAITSQ RQYMLRIELM DWEGNRAYSQ YDRFHIGNEK QNYRLYLKGH TGTAGKQSSL ILHGADFSTK DADNDNCMCK CALMLTGGWW FDACGPSNLN GMFYTAGQNH GKLNGIKWHY FKGPSYSLRS TTMMIRPLDF

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Background

This factor was found in the conditioned medium of the human neuroepithelioma cell line SHEP1 and the mouse myoblast cell line C2C12ras. The cDNA encoding a protein of 498 amino acids was isolated by using a secretion-trap expression cloning procedure exploiting the presence of a signal sequence present in growth factors that are secreted by producer cells . Murine and human factors show 97 % identity at the amino acid level. For a related factor see: CDT6. The human gene has been mapped to chromosome 8q22.3-q23 .

The expression of angiopoietin-1 mRNA is downregulated by PDGF, EGF, IL1-beta, and TGF-beta .

Angiopoietin-1 is a ligand for the receptor-like tyrosine kinase designated TIE-2 . Binding of Angiopoietin-1 to its receptor induces tyrosine phosphorylation of the cytoplasmic receptor domain. A naturally occuring antagonist of Angiopoietin-1 binding to TIE-2 is Angiopoietin-2. Angiopoietin-1 also binds to the TIE-1 receptor and this interaction appears to be critical for the development of the right-hand side venous system. It is dispensable for the left-hand side venous system, suggesting that right-hand and left-hand side vascular networks are established early before asymmetrical features of the network become morphologically discernible Loughna and Sato, 2001).

Angiopoietin-1 does not directly promote the growth of cultured endothelial cells. Angiopoietin-1 is chemotactic for endothelial cells . Excess soluble TIE-2 receptors abolish the chemotactic response of endothelial cells toward angiopoietin-1. Angiopoietin-1 has been shown to counteract cell death by apoptosis in cultured endothelial cells . Angiopoietin-1 also acts as an apoptosis survival factor for endothelial cells and this effect is augmented by the presence of VEGF .

该因子存在于人神经上皮瘤细胞系 SHEP1 和小鼠成肌细胞系 C2C12ras 的条件培养基中。编码 498 个氨基酸的蛋白质的 cDNA 是通过使用分泌陷阱表达克隆程序分离的，该程序利用存在于由生产细胞分泌的生长因子中存在的信号序列。鼠科动物和人类因素在氨基酸水平上显示出 97% 的同一性。有关相关因素，请参阅：CDT6。人类基因已被映射到染色体 8q22.3-q23 。

血管生成素-1 mRNA 的表达被 PDGF、EGF、IL1-β 和 TGF-β 下调。

Angiopoietin-1 是受体样酪氨酸激酶的配体，称为 TIE-2。 Angiopoietin-1 与其受体的结合诱导细胞质受体结构域的酪氨酸磷酸化。血管生成素-1 与 TIE-2 结合的天然拮抗剂是血管生成素-2。 Angiopoietin-1 也与 TIE-1 受体结合，这种相互作用似乎对右侧静脉系统的发育至关重要。它对于左侧静脉系统是可有可无的，这表明右侧和左侧血管网络在网络的不对称特征成为形态学上可辨别的早期建立之前 Loughna 和 Sato，2001）。

Angiopoietin-1 不直接促进培养的内皮细胞的生长。 Angiopoietin-1 对内皮细胞具有趋化作用。过量的可溶性 TIE-2 受体消除了内皮细胞对血管生成素-1 的趋化反应。 Angiopoietin-1 已被证明可通过培养的内皮细胞中的细胞凋亡来抵消细胞死亡。 Angiopoietin-1 还充当内皮细胞的凋亡存活因子，并且这种作用因 VEGF 的存在而增强。

Angiopoietin-2 dose-dependently blocks directed migration toward Angiopoietin-1. Carlson et al have shown that Ang-1 binds rather selectively to vitronectin and that Ang-1 can directly support adhesion of human umbilical vein endothelial cells and fibroblasts in a process mediated by integrins.

The physiologic roles of Angiopoietin-1 and its receptor are limited to angiogenic processes that occur subsequent to the earlier vasculogenic and angiogenic actions of the VEGF family and their receptors. However, it is unlike most of the known angiogenesis factors such as VEGF and other classical endothelial cell growth factors in that addition of the factor to cultures of endothelial cells does not directly promote cell growth even though the TIE-2 receptor becomes activated. Angiopoietin-1 also appears to be incapable of inducing the formation of tubules by endothelial cells.

Angiopoietins can potentiate the effects of other angiogenic cytokines. An investigation of the impact of angiopoietins on neovascularization in vivo in the cornea micropocket assay of neovascularization demonstrates that neither Angiopoietin-1 nor Angiopoietin-2 alone promote neovascularization. Holash et al have shown that a subset of tumors initially grows by coopting existing host vessels. Regression of these vessels via a process that involves disruption of interactions between endothelial cells and smooth muscle cells as well as cell death by apoptosis of endothelial cells first causes loss of tumour cells before angiogenesis begins at the tumor margin and the tumor is rescued under the influence of VEGF, Angiopoietin-1, and probably other angiogenic stimuli.

The embryonic expression pattern of Angiopoietin-1 suggests that it plays a particularly important role in the developing heart. Angiopoietin-1 is expressed highly in the myocardial wall surrounding the endocardium expressing TIE-2. Expression of Angiopoietin-1 becomes much more widespread later in development.

血管生成素可以增强其他血管生成细胞因子的作用。在新血管形成的角膜微袋试验中对血管生成素对体内新血管形成的影响的研究表明，Angiopoietin-1 和 Angiopoietin-2 都不能单独促进新血管形成。 Holash 等人已经表明，一部分肿瘤最初是通过吸收现有的宿主血管来生长的。通过涉及破坏内皮细胞和平滑肌细胞之间的相互作用以及内皮细胞凋亡引起的细胞死亡的过程使这些血管退化，首先导致肿瘤细胞在肿瘤边缘开始血管生成之前损失，并且在影响下挽救肿瘤VEGF、血管生成素-1 和可能的其他血管生成刺激。

Angiopoietin-1 的胚胎表达模式表明它在发育中的心脏中起着特别重要的作用。血管生成素-1 在表达 TIE-2 的心内膜周围的心肌壁中高度表达。 Angiopoietin-1 的表达在发育后期变得更加普遍。

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ProSpec-ANGPT2 Human/CYT-1103

ProSpec ANGPT2 Human,ProSpec CYT-1103,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ANGPT2 aka Angiopoietin-2 竞争结合 TIE2 受体并阻断血管生成过程中 angiopoietin-1 aka ANGPT1 诱导的 TIE2 自磷酸化。 ANGPT-2 是一种天然存在的 ANGPT-1 拮抗剂。

ANGPT2 Human

Angiopoietin 2 Human Recombinant
CYT-1103
ProSpec-ANGPT2 Human/CYT-1103 2µg
ProSpec-ANGPT2 Human/CYT-1103 10µg
ProSpec-ANGPT2 Human/CYT-1103 100µg

Catalogue number

CYT-1103

Synonyms

Angiopoietin-2, Angiopoietin2, ANGPT2, ANG2, ANG-2, ANGPT-2.

Introduction

ANGPT2 aka Angiopoietin-2 competes for binding to the TIE2 receptor and blocks angiopoietin-1 aka ANGPT1 induced TIE2 autophosphorylation during vasculogenesis. ANGPT-2 is a naturally occurring antagonist of ANGPT-1. ANGPT2 induces tyrosine phosphorylation of TEK/TIE2 in the lack of Angiopoietin-2. In the deficiency of VEGF an angiogenic inducer, ANGPT-2 induces endothelial cell apoptosis resulting in vascular regression. ANGPT2 along with VEGF enable endothelial cell migration and proliferation, resulting in permissive angiogenic signal.

ANGPT2 aka Angiopoietin-2 竞争结合 TIE2 受体并阻断血管生成过程中 angiopoietin-1 aka ANGPT1 诱导的 TIE2 自磷酸化。 ANGPT-2 是一种天然存在的 ANGPT-1 拮抗剂。 ANGPT2 在缺乏血管生成素-2 的情况下诱导 TEK/TIE2 的酪氨酸磷酸化。 在血管生成诱导剂 VEGF 的缺乏中，ANGPT-2 诱导内皮细胞凋亡，导致血管退化。 ANGPT2 与 VEGF 一起使内皮细胞迁移和增殖，从而产生允许的血管生成信号。

Description

ANGPT2 produced in Chinese hamster ovary cells by recombinant DNA technology is a single, polypeptide chain and fused to a 6 aa His Tag at C-terminus containing a total of 484 amino acids and having a molecular mass of 55.7kDa.
ANGPT2 shows multiple bands between 50-100kDa on -PAGE, reducing conditions and purified by proprietary chromatographic techniques.

通过重组DNA技术在中国仓鼠卵巢细胞中产生的ANGPT2是一条多肽链，在C端融合了一个6个氨基酸的His标签，共含有484个氨基酸，分子量为55.7kDa。
ANGPT2 在 -PAGE、还原条件和专有色谱技术纯化后显示 50-100kDa 之间的多个条带。

Source

Chinese Hamster Ovary Cells .

Physical Appearance

Sterile filtered colorless solution.

Formulation

ANGPT2 protein solution contains Phosphate buffered saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

YNNFRKSMDS IGKKQYQVQH GSCSYTFLLP EMDNCRSSSS PYVSNAVQRD APLEYDDSVQ
RLQVLENIME NNTQWLMKLE NYIQDNMKKEMVEIQQNAVQ NQTAVMIEIG TNLLNQTAEQ
TRKLTDVEAQ VLNQTTRLEL QLLEHSLSTN KLEKQILDQT SEINKLQDKN
SFLEKKVLAMEDKHIIQLQS IKEEKDQLQV LVSKQNSIIE ELEKKIVTAT VNNSVLQKQQ
HDLMETVNNL LTMMSTSNSA KDPTVAKEEQ ISFRDCAEVFKSGHTTNGIY TLTFPNSTEE
IKAYCDMEAG GGGWTIIQRR EDGSVDFQRT WKEYKVGFGN PSGEYWLGNE FVSQLTNQQR
YVLKIHLKDWEGNEAYSLYE HFYLSSEELN YRIHLKGLTG TAGKISSISQ PGNDFSTKDG
DNDKCICKCS QMLTGGWWFD ACGPSNLNGM YYPQRQNTNKFNGIKWYYWK GSGYSLKATT MMIRPADFHH HHHH

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-ANGPTL2 Human/CYT-765

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ANGPTL2 Human

Angiopoietin-like Protein 2 Human Recombinant
CYT-765
ProSpec-ANGPTL2 Human/CYT-765 2µg
ProSpec-ANGPTL2 Human/CYT-765 10µg
ProSpec-ANGPTL2 Human/CYT-765 1mg

Catalogue number

CYT-765

Synonyms

Angiopoietin-related protein 2, Angiopoietin-like protein 2, ANGPTL2, ARP2, HARP.
血管生成素相关蛋白 2、血管生成素样蛋白 2、ANGPTL2、ARP2、HARP。

Introduction

Angiopoietins belong to the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietins-1, 2 and 4 partake in the formation of blood vessels. ANGPTL2 displays angiogenic effects. Angiopoietin-like Protein 2 is an anti-diabetic factor. ANGPTL2 induces sprouting in endothelial cells through an autocrine and paracrine action. ANGPTL2 increases insulin sensitivity in adipocytes. In addition, ANGPTL2 is a mediator of chronic adipose tissue inflammation. ANGPTL2 is widely expressed in the heart, small intestine, spleen and stomach. ANGPTL2 is also found in lower levels in the colon, ovary, adrenal gland, skeletal muscle and in prostate.
血管生成素属于血管内皮生长因子家族，是唯一已知的主要对血管内皮特异的生长因子。 血管生成素-1、2 和 4 参与血管的形成。 ANGPTL2 显示血管生成作用。 血管生成素样蛋白 2 是一种抗糖尿病因子。 ANGPTL2 通过自分泌和旁分泌作用诱导内皮细胞发芽。 ANGPTL2 增加脂肪细胞的胰岛素敏感性。 此外，ANGPTL2 是慢性脂肪组织炎症的介质。 ANGPTL2在心脏、小肠、脾和胃中广泛表达。 ANGPTL2 也存在于结肠、卵巢、肾上腺、骨骼肌和前列腺中的较低水平。

Description

ANGPTL2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 481 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 56kDa .
在大肠杆菌中产生的 ANGPTL2 Human Recombinant 是一个单一的、非糖基化的多肽链，包含 481 个氨基酸，包括一个 10 个氨基酸的 N 端 His 标签。 总分子量为56kDa。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

ANGPTL2 was filtered and lyophilized from 0.5mg/ml in 0.03M Acetate buffer pH 4.0.

Solubility

It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, an intensive dilution by relevant buffer to a concentration of 10?g/ml is recommended. In higher concentrations the solubility of the ANGPTL2 is limited. ANGPTL2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入 0.1M 醋酸盐缓冲液 pH-4 制备约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，建议使用相关缓冲液将其充分稀释至 10?g/ml 的浓度。 在较高浓度下，ANGPTL2 的溶解度是有限的。 ANGPTL2 不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHASQEDGFEGTEE GSPREFIYLN RYKRAGESQD KCTYTFIVPQ QRVTGAICVN SKEPEVLLEN RVHKQELELL NNELLKQKRQ IETLQQLVEV DGGIVSEVKL LRKESRNMNS RVTQLYMQLL HEIIRKRDNA LELSQLENRI LNQTADMLQL ASKYKDLEHK YQHLATLAHN QSEIIAQLEE HCQRVPSARP VPQPPPAAPP RVYQPPTYNR IINQISTNEI QSDQNLKVLP PPLPTMPTLT SLPSSTDKPS GPWRDCLQAL EDGHDTSSIY LVKPENTNRL MQVWCDQRHD PGGWTVIQRR LDGSVNFFRN WETYKQGFGN IDGEYWLGLE NIYWLTNQGN YKLLVTMEDW SGRKVFAEYA SFRLEPESEY YKLRLGRYHG NAGDSFTWHN GKQFTTLDRD HDVYTGNCAH YQKGGWWYNA CAHSNLNGVW YRGGHYRSRY QDGVYWAEFR GGSYSLKKVV MMIRPNPNTF H.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-ANGPTL3 (17-460) Human/CYT-986

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ANGPTL3 Human

Angiopoietin-like Protein 3 Human Recombinant
CYT-986
ProSpec-ANGPTL3 (17-460) Human/CYT-986 2µg
ProSpec-ANGPTL3 (17-460) Human/CYT-986 10µg
ProSpec-ANGPTL3 (17-460) Human/CYT-986 1mg

Catalogue number

CYT-986

Synonyms

Angiopoietin Like 3, Angiopoietin 5, ANGPT5, ANG-5, Angiopoietin-Related Protein 3 , Angiopoietin-Like Protein 3, Angiopoietin-Like 3, Angiopoietin-5, FHBL2, ANL3.
血管生成素样 3、血管生成素 5、ANGPT5、ANG-5、血管生成素相关蛋白 3、血管生成素样蛋白 3、血管生成素样 3、血管生成素-5、FHBL2、ANL3。

Introduction

ANGPTL3 and ANGPTL4 are angiopoietin-like proteins secreted and expressed mainly by the liver, their role being the regulation of triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. During different nutritional states the levels of the circulating triglycerides are regulated by Angptl3 and Angptl4 through differential inhibition of Lipoprotein lipase as shown by the experimental data. The molecular structure of ANGPTL3 is similar to that of the angiopoietins . Deletion mutants of human Angiopoietin 5 were used in order to demonstrate that the N-terminal domain and not the C-terminal fibrinogen-like domain increased the plasma triglyceride levels in mice.
ANGPTL3和ANGPTL4是主要由肝脏分泌和表达的血管生成素样蛋白，它们的作用是通过抑制富含甘油三酯的脂蛋白的脂解来调节甘油三酯代谢。 如实验数据所示，在不同的营养状态期间，循环甘油三酯的水平由 Angptl3 和 Angptl4 通过对脂蛋白脂肪酶的差异抑制来调节。 ANGPTL3 的分子结构与血管生成素相似。 使用人血管生成素 5 的缺失突变体来证明 N 端结构域而不是 C 端纤维蛋白原样结构域增加了小鼠血浆甘油三酯水平。

Description

ANGPTL3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 453 amino acids and having a molecular mass of 52.9kDa .
ANGPTL3 is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

ANGPTL3 protein solution contains Buffered Saline ,30% glycerol And 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

ADPSRIDQDN SSFDSLSPEP KSRFAMLDDV KILANGLLQL GHGLKDFVHK TKGQINDIFQ KLNIFDQSFY DLSLQTSEIK EEEKELRRTT YKLQVKNEEV KNMSLELNSK LESLLEEKIL LQQKVKYLEE QLTNLIQNQP ETPEHPEVTS LKTFVEKQDN SIKDLLQTVE DQYKQLNQQH SQIKEIENQL RRTSIQEPTE ISLSSKPRAP RTTPFLQLNE IRNVKHDGIP AECTTIYNRG EHTSGMYAIR PSNSQVFHVY CDVISGSPWT LIQHRIDGSQ NFNETWENYK YGFGRLDGEF WLGLEKIYSI VKQSNYVLRI ELEDWKDNKH YIEYSFYLGN HETNYTLHLV AITGNVPNAI PENKDLVFST WDHKAKGHFN CPEGYSGGWW WHDECGENNL NGKYNKPRAK SKPERRRGLS WKSQNGRLYS IKSTKMLIHP TDSESFEHHH HHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • ANGPTL3 Antibody

ProSpec-ANGPTL3 (243-460) Human/CYT-902

ProSpec ANGPTL3 (243-460) Human,ProSpec CYT-902,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ANGPTL3和ANGPTL4是主要由肝脏分泌和表达的血管生成素样蛋白，它们的作用是通过抑制富含甘油三酯的脂蛋白的脂解来调节甘油三酯代谢。

ANGPTL3 Human

Angiopoietin-like Protein 3 Human Recombinant
CYT-902
ProSpec-ANGPTL3 (243-460) Human/CYT-902 5µg
ProSpec-ANGPTL3 (243-460) Human/CYT-902 20µg
ProSpec-ANGPTL3 (243-460) Human/CYT-902 1mg

Catalogue number

CYT-902

Synonyms

Angiopoietin-Like 3, ANGPT5, Angiopoietin 5, FHBL2, ANG-5, Angiopoietin-Related Protein 3, Angiopoietin-Like Protein 3, Angiopoietin-5, ANL3, Angiopoietin-related protein 3.

Introduction

ANGPTL3 and ANGPTL4 are angiopoietin-like proteins secreted and expressed mainly by the liver, their role being the regulation of triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. During different nutritional states the levels of the circulating triglycerides are regulated by Angptl3 and Angptl4 through differential inhibition of Lipoprotein lipase as shown by the experimental data. The molecular structure of ANGPTL3 is similar to that of the angiopoietins . Deletion mutants of human Angiopoietin 5 were used in order to demonstrate that the N-terminal domain and not the C-terminal fibrinogen-like domain increased the plasma triglyceride levels in mice.
ANGPTL3和ANGPTL4是主要由肝脏分泌和表达的血管生成素样蛋白，它们的作用是通过抑制富含甘油三酯的脂蛋白的脂解来调节甘油三酯代谢。 如实验数据所示，在不同的营养状态期间，循环甘油三酯的水平由 Angptl3 和 Angptl4 通过对脂蛋白脂肪酶的差异抑制来调节。 ANGPTL3 的分子结构与血管生成素相似。 使用人血管生成素 5 的缺失突变体来证明 N 端结构域而不是 C 端纤维蛋白原样结构域增加了小鼠血浆甘油三酯水平。

Description

ANGPTL3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 239 amino acids and having a molecular mass of 27.7kDa.

ANGPTL3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

ANGPTL3 Human Recombinant 在大肠杆菌中产生，是一个单一的、非糖基化的多肽链，含有 239 个氨基酸，分子量为 27.7kDa。

ANGPTL3 在 N 端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

ANGPTL3 protein solution containing 20mM Tris-HCl and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MPAECTTIYN RGEHTSGMYA IRPSNSQVFH VYCDVISGSP WTLIQHRIDG SQNFNETWEN YKYGFGRLDG EFWLGLEKIY SIVKQSNYVL RIELEDWKDN KHYIEYSFYL GNHETNYTLH LVAITGNVPN AIPENKDLVF STWDHKAKGH FNCPEGYSGG WWWHDECGEN NLNGKYNKPR AKSKPERRRG LSWKSQNGRL YSIKSTKMLI HPTDSESFE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • ANGPTL3 Antibody
  • ANGPTL3 Human
  • ANGPTL3 Human
  • ANGPTL3 Human, HEK
  • ANGPTL3 Antibody

ProSpec-ANGPTL3 Human/CYT-248

ProSpec ANGPTL3 Human,ProSpec CYT-248,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,加入 0.1M 醋酸缓冲液 pH-4，让 ANGPTL3 Human 冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10μg/ml 的浓度。 在较高浓度下，血管生成素 5 的溶解度是有限的。

ANGPTL3 Human

Angiopoietin Like Protein 3 Human Recombinant
CYT-248
ProSpec-ANGPTL3 Human/CYT-248 2µg
ProSpec-ANGPTL3 Human/CYT-248 10µg
ProSpec-ANGPTL3 Human/CYT-248 1mg

Catalogue number

CYT-248

Synonyms

Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.

Introduction

ANGPTL3 and ANGPTL4 are angiopoietin-like proteins secreted and expressed mainly by the liver, their role being the regulation of triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. During different nutritional states the levels of the circulating triglycerides are regulated by Angptl3 and Angptl4 through differential inhibition of Lipoprotein lipase as shown by the experimental data. The molecular structure of ANGPTL3 is similar to that of the angiopoietins . Deletion mutants of human Angiopoietin 5 were used in order to demonstrate that the N-terminal domain and not the C-terminal fibrinogen-like domain increased the plasma triglyceride levels in mice.
ANGPTL3和ANGPTL4是主要由肝脏分泌和表达的血管生成素样蛋白，它们的作用是通过抑制富含甘油三酯的脂蛋白的脂解来调节甘油三酯代谢。 如实验数据所示，在不同的营养状态期间，循环甘油三酯的水平由 Angptl3 和 Angptl4 通过对脂蛋白脂肪酶的差异抑制来调节。 ANGPTL3 的分子结构与血管生成素相似。 使用人血管生成素 5 的缺失突变体来证明 N 端结构域而不是 C 端纤维蛋白原样结构域增加了小鼠血浆甘油三酯水平。

Description

The ANGPTL3 Human Recombinant is produced with N-terminal fusion of His-Tag. The Angiopoietin-like protein 3 His Tagged Fusion Protein is 26kDa containing 207 amino acid residues of the ANGPTL3 Human and 16 additional amino acid residues – His-Tag .
ANGPTL3 Human Recombinant 是由 His-Tag 的 N 端融合产生的。 血管生成素样蛋白 3 His 标签的融合蛋白是 26kDa，含有 ANGPTL3 Human 的 207 个氨基酸残基和 16 个额外的氨基酸残基 – His-Tag。

Source

Escherichia Coli.

Formulation

ANGPTL3 Human filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Purity

Angiopoietin 5 purity is greater than 95% as determined by -PAGE.

Solubility

Add 0.1M Acetate buffer pH-4 and let the lyophilized pellet of ANGPTL3 Human dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of Angiopoietin 5 is limited.

加入 0.1M 醋酸缓冲液 pH-4，让 ANGPTL3 Human 冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10μg/ml 的浓度。 在较高浓度下，血管生成素 5 的溶解度是有限的。

Stability

Store lyophilized ANGPTL3 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Angiopoietin 5 can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MRGSHHHHHH GMASHMSRID QDNSSFDSLS PEPKSRFAML DDVKILANGL LQLGHGLKDF VHKTKGQIND IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL NSKLESLLEE KILLQQKVKY LEEQLTNLIQ NQPETPEHPE VTSLKTFVEK QDNSIKDLLQ TVEDQYKQLN QQHSQIKEIE NQLRRTSIQE PTEISLSSKP RAP.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • ANGPTL3 Antibody
  • ANGPTL3 Human
  • ANGPTL3 Human
  • ANGPTL3 Human, HEK
  • ANGPTL3 Antibody

ProSpec-ANGPTL3 Human, HEK/CYT-766

ProSpec ANGPTL3 Human HEK,CYT-766,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,建议加入去离子水至工作浓度0.5mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

ANGPTL3 Human, HEK

Angiopoietin Like Protein 3 Human Recombinant, HEK
CYT-766
ProSpec-ANGPTL3 Human, HEK/CYT-766 2µg
ProSpec-ANGPTL3 Human, HEK/CYT-766 10µg
ProSpec-ANGPTL3 Human, HEK/CYT-766 100µg

Catalogue number

CYT-766

Synonyms

Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3.

Introduction

ANGPTL3 and ANGPTL4 are angiopoietin-like proteins secreted and expressed mainly by the liver, their role being the regulation of triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. During different nutritional states the levels of the circulating triglycerides are regulated by Angptl3 and Angptl4 through differential inhibition of Lipoprotein lipase as shown by the experimental data. The molecular structure of ANGPTL3 is similar to that of the angiopoietins . Deletion mutants of human Angiopoietin 5 were used in order to demonstrate that the N-terminal domain and not the C-terminal fibrinogen-like domain increased the plasma triglyceride levels in mice.
ANGPTL3和ANGPTL4是主要由肝脏分泌和表达的血管生成素样蛋白，它们的作用是通过抑制富含甘油三酯的脂蛋白的脂解来调节甘油三酯代谢。 如实验数据所示，在不同的营养状态期间，循环甘油三酯的水平由 Angptl3 和 Angptl4 通过对脂蛋白脂肪酶的差异抑制来调节。 ANGPTL3 的分子结构与血管生成素相似。 使用人血管生成素 5 的缺失突变体来证明 N 端结构域而不是 C 端纤维蛋白原样结构域增加了小鼠血浆甘油三酯水平。

Description

ANGPTL3 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain containing a total of 450 amino acids, having a molecular mass of 52.6kDa and fused to a 6 aa His tag at C-Terminus.
The Human ANGPTL3 is purified by proprietary chromatographic techniques.
在 HEK 细胞中产生的 ANGPTL3 Human Recombinant 是一条单一的糖基化多肽链，总共包含 450 个氨基酸，分子量为 52.6kDa，并在 C 末端与一个 6aa His 标签融合。
人 ANGPTL3 通过专有的色谱技术进行纯化。

Source

HEK 293.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.

Solubility

It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
建议加入去离子水至工作浓度0.5mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间。

Amino acid sequence

SRIDQDNSSF DSLSPEPKSR FAMLDDVKIL ANGLLQLGHG LKDFVHKTKG QINDIFQKLN IFDQSFYDLS LQTSEIKEEE KELRRTTYKL QVKNEEVKNM SLELNSKLES LLEEKILLQQ KVKYLEEQLT NLIQNQPETP EHPEVTSLKT FVEKQDNSIK DLLQTVEDQY KQLNQQHSQI KEIENQLRRT SIQEPTEISL SSKPRAPRTT PFLQLNEIRN VKHDGIPAEC TTIYNRGEHT SGMYAIRPSN SQVFHVYCDV ISGSPWTLIQ HRIDGSQNFN ETWENYKYGF GRLDGEFWLG LEKIYSIVKQ SNYVLRIELE DWKDNKHYIE YSFYLGNHET NYTLHLVAIT GNVPNAIPEN KDLVFSTWDH KAKGHFNCPE GYSGGWWWHD ECGENNLNGK YNKPRAKSKP ERRRGLSWKS QNGRLYSIKS TKMLIHPTDS ESFEHHHHHH.

Purity

Greater than 95.0% as determined by -PAGE.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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  • ANGPTL3 Human
  • ANGPTL3 Human
  • ANGPTL3 Antibody
  • ANGPTL3 Human
  • ANGPTL3 Antibody

ProSpec-ANGPTL4 Human/CYT-249

ProSpec ANGPTL4 Human,ProSpec CYT-249,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ANGPTL4、NL2、ARP4、FIAF、PGAR、HFARP、pp1158、ANGPTL2、空腹诱导脂肪因子、肝纤维蛋白原/血管生成素相关蛋白、PPARG 血管生成素相关蛋白。

ANGPTL4 Human

Angiopoietin-like Protein 4 Human Recombinant
CYT-249
ProSpec-ANGPTL4 Human/CYT-249 2µg
ProSpec-ANGPTL4 Human/CYT-249 10µg
ProSpec-ANGPTL4 Human/CYT-249 1mg

Catalogue number

CYT-249

Synonyms

ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.
ANGPTL4、NL2、ARP4、FIAF、PGAR、HFARP、pp1158、ANGPTL2、空腹诱导脂肪因子、肝纤维蛋白原/血管生成素相关蛋白、PPARG 血管生成素相关蛋白。

Introduction

FIAF a.k.a ANGPTL4 or PGAR or HFARP is an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. ANGPTL4 is found in human and mouse blood plasma both as a native protein and in a truncated form. In human white adipose tissue and SGBS adipocytes, only the native form of ANGPTL4 could be detected, whereas in mice the differentiation of mouse 3T3-L1 adipocytes is associated with the production of truncated ANGPTL4. However, the truncated ANGPTL4 is produced by human liver. In human blood plasma FIAF is mainly presented in a truncated form , whose levels treatment increases . There is an inter individual variation in ANGPTL4 levels of both the truncated and the native form, however those levels were not influenced by prolonged semistarvation and are not associated with body mass index.

FIAF a.k.a ANGPTL4 或 PGAR 或 HFARP 是一种通过禁食、过氧化物酶体增殖物激活受体激动剂和缺氧上调的脂肪细胞因子。 ANGPTL4 以天然蛋白质和截短形式存在于人和小鼠血浆中。 在人类白色脂肪组织和 SGBS 脂肪细胞中，只能检测到天然形式的 ANGPTL4，而在小鼠中，小鼠 3T3-L1 脂肪细胞的分化与截短 ANGPTL4 的产生有关。 然而，截短的 ANGPTL4 是由人肝脏产生的。 在人血浆中，FIAF 主要以截短形式呈现，其水平治疗增加。 截断和天然形式的 ANGPTL4 水平存在个体间差异，但这些水平不受长期半饥饿的影响，并且与体重指数无关。

Description

The ANGPTL4 Human Recombinant is manufactured with N-terminal fusion of His Tag.
The Angiopoietin-like Protein 4 His -Tagged Fusion Protein is 25 kDa protein containing 204 amino acid residues of the Angiopoietin-like Protein 4 and 16 additional amino acid residues – His Tag .
ANGPTL4 Human Recombinant 是用 His 标签的 N 端融合制造的。
血管生成素样蛋白 4 His-Tagged 融合蛋白是 25 kDa 的蛋白质，含有血管生成素样蛋白 4 的 204 个氨基酸残基和 16 个额外的氨基酸残基 – His Tag。

Source

Escherichia Coli.

Purity

Greater than 95% as determined by -PAGE.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Solubility

Add 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.

加入 0.1M 醋酸缓冲液 pH4，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10μg/ml 的浓度。 在较高浓度下，该抗原的溶解度是有限的。

Stability

Store lyophilized Angiopoietin-like Protein 4 Human recombinant at -20°C. Aliquot the ANGPTL4 after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted ANGPTL4 can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的血管生成素样蛋白 4 人重组体储存在 -20°C。 重组后分装 ANGPTL4，以避免重复冷冻/解冻循环。 重构的 ANGPTL4 可以在 4°C 下储存一段有限的时间； 在 4°C 下两周后它没有显示任何变化。

Amino acid sequence

MRGSHHHHHH GMASHMGPVQ SKSPRFASWD EMNVLAHGLL QLGQGLREHA ERTRSQLSAL ERRLSACGSA CQGTEGSTDL PLAPESRVDP EVLHSLQTQL KAQNSRIQQL FHKVAQQQRH LEKQHLRIQH LQSQFGLLDH KHLDHEVAKP ARRKRLPEMA QPVDPAHNVS RLHRLPRDCQ ELFQVGERQS GLFEIQPQGS PPFLVNCKMT SDGGWTVIQR.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • ANGPTL4 Human, HEK

ProSpec-ANGPTL4 Human, HEK/CYT-698

ProSpec ANGPTL4 Human HEK,ProSpec CYT-698,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.

ANGPTL4 Human, HEK

Angiopoietin-like Protein 4 Human Recombinant, HEK
CYT-698
ProSpec-ANGPTL4 Human, HEK/CYT-698 2µg
ProSpec-ANGPTL4 Human, HEK/CYT-698 10µg
ProSpec-ANGPTL4 Human, HEK/CYT-698 1mg

Catalogue number

CYT-698

Synonyms

ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.

Introduction

The fasting-induced adipose factor was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, FIAF was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated FIAF, whereas in human white adipose tissue and SGBS adipocytes, only the native FIAF could be detected. Interestingly, the truncated FIAF was produced by human liver.
Experimental data suggest that FIAF is mainly presented in human blood plasma in a truncated form , whose level is increased by fenofibrate treatment. Levels of both truncated and native FIAF showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation.
禁食诱导的脂肪因子被确定为通过禁食、过氧化物酶体增殖物激活受体激动剂和缺氧上调的脂肪细胞因子。 在蛋白质水平，在人和小鼠血浆中，发现 FIAF 以天然蛋白质和截短形式存在。 小鼠 3T3-L1 脂肪细胞的分化与截短 FIAF 的产生有关，而在人类白色脂肪组织和 SGBS 脂肪细胞中，只能检测到天然 FIAF。 有趣的是，截断的 FIAF 是由人类肝脏产生的。
实验数据表明，FIAF主要以截短形式存在于人血浆中，其水平因非诺贝特治疗而增加。 截断和原生 FIAF 的水平显示出明显的个体差异，但与体重指数无关，也不受长期半饥饿的影响。

Description

The ANGPTL4 Human Recombinant is manufactured with C-terminal fusion of 11 amino acid FLAG Tag.
The ANGPTL4 Flag -Tagged Fusion Protein is a 44.2kDa protein containing 392 amino acid residues of the Angiopoietin-like Protein 4 and 11 additional amino acid residues – Flag Tag .
ANGPTL4 Human Recombinant 由 11 个氨基酸 FLAG 标签的 C 端融合制成。
ANGPTL4 Flag-Tagged 融合蛋白是一种 44.2kDa 的蛋白质，含有血管生成素样蛋白 4 的 392 个氨基酸残基和 11 个额外的氨基酸残基 – Flag Tag。

Source

HEK293.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.

Solubility

Add pyrogen free water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
加入无热原水至 0.5mg/ml 的工作浓度，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized Angiopoietin-like Protein 4 Human recombinant at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的血管生成素样蛋白 4 人重组体储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

GPVQSKSPRF ASWDEMNVLA HGLLQLGQGL REHAERTRSQ LSALERRLSA CGSACQGTEG STDLPLAPES RVDPEVLHSL QTQLKAQNSR IQQLFHKVAQ QQRHLEKQHL RIQHLQSQFG LLDHKHLDHE VAKPARRKRL PEMAQPVDPA HNVSRLHRLP RDCQELFQVG ERQSGLFEIQ PQGSPPFLVN CKMTSDGGWT VIQRRHDGSV DFNRPWEAYK AGFGDPHGEF WLGLEKVHSI TGDRNSRLAV QLRDWDGNAE LLQFSVHLGG EDTAYSLQLT APVAGQLGAT TVPPSGLSVP FSTWDQDHDL RRDKNCAKSL SGGWWFGTCS HSNLNGQYFR SIPQQRQKLK KGIFWKTWRG RYYPLQATTM LIQPMAAEAA SAAADYKDDDDK.

Applications

Western blotting.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • ANGPTL4 Human

ProSpec-ANGPTL6 Human/CYT-832

ProSpec ANGPTL6 Human,ProSpec CYT-832,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ANGPTL6 Human Recombinant 在大肠杆菌中产生，是一条包含 460 个氨基酸的多肽链，总分子量为 50.7 kDa。 ANGPTL6 包含一个 N-末端 10 aa His-Tag。

ANGPTL6 Human

Angiopoietin-like Protein 6 Human Recombinant
CYT-832
ProSpec-ANGPTL6 Human/CYT-832 2µg
ProSpec-ANGPTL6 Human/CYT-832 10µg
ProSpec-ANGPTL6 Human/CYT-832 1mg

Catalogue number

CYT-832

Synonyms

Angiopoietin-Like Protein 6, Angiopoietin-Related Protein 5, Angiopoietin-Related Growth Factor, Angiopoietin-Related Protein 6, AGF, ARP5.
血管生成素样蛋白 6、血管生成素相关蛋白 5、血管生成素相关生长因子、血管生成素相关蛋白 6、AGF、ARP5。

Introduction

ANGPTL6 is a protein-coding gene which takes part is several processes. ANGPTL6 is involved in the wound healing process, promotes the chemotactic activity of endothelial cells and induce neovascularization. ANGPTL6 also counteracts high-fat diet-induced obesity and related insulin resistance through increased energy expenditure. Additionally ANGPTL6 promotes epidermal proliferation, remodeling and regeneration. Diseases linked with ANGPTL6 include enterobiasis, and gnathomiasis.
ANGPTL6是一个参与多个过程的蛋白质编码基因。 ANGPTL6参与伤口愈合过程，促进内皮细胞的趋化活性并诱导新生血管形成。 ANGPTL6 还通过增加能量消耗来抵消高脂饮食引起的肥胖和相关的胰岛素抵抗。 此外，ANGPTL6 促进表皮增殖、重塑和再生。 与 ANGPTL6 相关的疾病包括蛲虫病和颌吸虫病。

Description

ANGPTL6 Human Recombinant produced in E.coli is a polypeptide chain containing 460 amino acids and having a total molecular mass of 50.7 kDa. ANGPTL6 contains a N-Terminal 10 aa His-Tag.
ANGPTL6 Human Recombinant 在大肠杆菌中产生，是一条包含 460 个氨基酸的多肽链，总分子量为 50.7 kDa。 ANGPTL6 包含一个 N-末端 10 aa His-Tag。

Source

E.coli.

Physical Appearance

White lyophilized powder.

Formulation

Filtered and lyophilized from a 0.5mg/ml solution containing 0.05M Acetate buffer

pH 4.0.

Solubility

Add 0.1M acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10?g/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
加入 0.1M 醋酸盐缓冲液 pH 4.0 制备 0.5 mg/ml 的工作储备溶液，让冻干颗粒完全溶解。 为了转化为更高的 pH 值，我们建议使用相关缓冲液将其充分稀释至 10?g/ml 的浓度。 在较高浓度下，该抗原的溶解度是有限的。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Lyophilized ANGPTL6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ANGPTL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
冻干的 ANGPTL6 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，ANGPTL6 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

MKHHHHHHAS RAGAPRCTYT FVLPPQKFTG AVCWSGPAST RATPEAANAS ELAALRMRVG RHEELLRELQ RLAAADGAVA GEVRALRKES RGLSARLGQL RAQLQHEAGP GAGPGADLGA EPAAALALLG ERVLNASAEA QRAAARFHQL DVKFRELAQL VTQQSSLIAR LERLCPGGAG GQQQVLPPPP LVPVVPVRLV GSTSDTSRML DPAPEPQRDQ TQRQQEPMAS PMPAGHPAVP TKPVGPWQDC AEARQAGHEQ SGVYELRVGR HVVSVWCEQQ LEGGGWTVIQ RRQDGSVNFF TTWQHYKAGF GRPDGEYWLG LEPVYQLTSR GDHELLVLLE DWGGRGARAH YDGFSLEPES DHYRLRLGQY HGDAGDSLSW HNDKPFSTVD RDRDSYSGNC ALYQRGGWWY HACAHSNLNG VWHHGGHYRS RYQDGVYWAE FRGGAYSLRK AAMLIRPLKL

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-ANGPTL7 Mouse/CYT-914

ProSpec ANGPTL7 Mouse,ProSpec CYT-914,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Angiopoietin-related protein 7, Angiopoietin-like protein 7.

ANGPTL7 Mouse

Angiopoietin-like Protein 7 Mouse Recombinant
CYT-914
ProSpec-ANGPTL7 Mouse/CYT-914 1µg
ProSpec-ANGPTL7 Mouse/CYT-914 5µg
ProSpec-ANGPTL7 Mouse/CYT-914 25µg

Catalogue number

CYT-914

Synonyms

Angiopoietin-related protein 7, Angiopoietin-like protein 7.

Introduction

Angiopoietin-related protein 7 , belongs to the angiopoietin-like family of molecules. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. ANGPTL7 production is up-regulated in trabecular meshwork cells by glucocorticoids and TGF-Beta and in cartilage by TNF-alpha.
血管生成素相关蛋白 7 ，属于血管生成素样分子家族。 ANGPTL7 在角膜基质、小梁网和巩膜中表达。 ANGPTL7 的产生在小梁网细胞中被糖皮质激素和 TGF-β 上调，在软骨中被 TNF-α 上调。

Description

ANGPTL7 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 324 amino acids and having a molecular mass of 37.5kDa.
ANGPTL7 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
在 Sf9 杆状病毒细胞中产生的 ANGPTL7 是单条糖基化多肽链，含有 324 个氨基酸，分子量为 37.5kDa。
ANGPTL7 在 C 末端用 8 个氨基酸的 His 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

ANGPTL7 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein .

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。

对于长期储存，建议添加载体蛋白。

Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by -PAGE.

Amino acid sequence

QKPHKRKTQL KAAGCCEEMR ELKAQVANLS SLLGELSRKQ ESDWVSVVMQ VMELESSSKH MESRLSTAES KYSEMNNQID IMQLQAAQTV TQTSADAIYD CSSLYQKNYR ISGVYKLPPD EFLGSPELEV FCDMETSGGG WTIIQRRKSG LVSFYQDWRQ YKQGFGSIRG DFWLGNEHIH RLTRQPSRLR VELEDWEGNA RYAEYSYFAL GNELNSYRLF LGNYSGNVGK DALLYHNNTV FSTKDKDNDN CLDKCAQLRK GGYWYNCCTD SNLNGVYYRL GEHRKHMDGI SWYGWHGANY SLKRVEMKIR PEAFKPLEHH HHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-Apo D Human/CYT-547

ProSpec Apo D Human,ProSpec CYT-547,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,建议加入去离子水至 0.5mg/ml 的工作体积，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤本产品。

Apo D Human

Apolipoprotein-D Human Recombinant
CYT-547
ProSpec-Apo D Human/CYT-547 2µg
ProSpec-Apo D Human/CYT-547 10µg
ProSpec-Apo D Human/CYT-547 1mg

Catalogue number

CYT-547

Synonyms

Apolipoprotein D, Apo-D, ApoD.

Introduction

Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid.
Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimer’s disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue.
载脂蛋白-D主要与人血浆中的高密度脂蛋白有关。载脂蛋白-D 是一种非典型载脂蛋白，根据其一级结构，载脂蛋白-D 是载脂蛋白家族的成员。脂质运载蛋白采用β-桶三级结构并运输小的疏水配体。载脂蛋白-D 结合胆固醇、孕酮、孕烯醇酮、胆红素和花生四烯酸。
载脂蛋白-D在脾脏、睾丸和脑中具有高水平表达的许多组织中表达。载脂蛋白-D 以高浓度存在于患有严重乳腺囊性疾病的女性的囊液中，这种疾病与乳腺癌风险增加有关。载脂蛋白-D 积聚在再生的周围神经和患有神经退行性疾病（如阿尔茨海默病）患者的脑脊液中。载脂蛋白-D 参与中枢和外周神经系统的维护和修复。载脂蛋白-D 是一种多配体、多功能转运蛋白，可将配体从一个细胞转运到器官内的另一个细胞，清除器官内的配体以转运到血液中，或者可以将配体从循环转运到器官内的特定细胞组织。

Description

Apolipoprotein-D Human Recombinant His Tag fusion protein at C-terminus produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.82kDa.
The protein a.a sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.
The Following gene modifications were made:
Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apolipoprotein-D to enhance the protein’s solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchanges which facilitate its genetic manipulation. The Apolipoprotein-D is purified by proprietary chromatographic techniques.
载脂蛋白-D 人重组 His Tag 融合蛋白在 C 端产生于大肠杆菌中，是一种单一的、非糖基化的多肽链，含有 174 个氨基酸，分子量为 19.82kDa。
蛋白质 a.a 序列对应于 UniProtKB/Swiss-Prot 条目 P05090。
进行了以下基因修饰：
在载脂蛋白-D 表面引入 Trp99His、Cys116Ser、Ile118Ser、Leu120Ser 氨基酸交换以增强蛋白质的溶解度，另外三个 Leu23Pro、Pro133Val、Asn134Ala 氨基酸交换有助于其遗传操作。 载脂蛋白-D 是通过专有的色谱技术纯化的。

Source

Escherichia Coli.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 1mg/ml in 4mM KH2PO4, 16mM Na2HPO4 and 115mM NaCl pH 7.5.

Solubility

It is recommended to add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by an appropriate sterile filter before using it in the cell culture.
建议加入去离子水至 0.5mg/ml 的工作体积，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤本产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • APO D Human, GST
  • APOD Human, HEK

ProSpec-APOA5 Human/CYT-767

ProSpec APOA5 Human,ProSpec CYT-767,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,ApoA5 是血清甘油三酯浓度的重要调节剂。 ApoA5 小鼠敲除模型产生的血清甘油三酯增加约四倍，而敲入模型人 ApoA5 产生的小鼠血清甘油三酯浓度降低了 50-70%。

APOA5 Human

Apolipoprotein A-V Human Recombinant
CYT-767
ProSpec-APOA5 Human/CYT-767 2µg
ProSpec-APOA5 Human/CYT-767 10µg
ProSpec-APOA5 Human/CYT-767 1mg

Catalogue number

CYT-767

Synonyms

Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3, APOA5, RAP3, APOAV.

Introduction

ApoA5 is an important regulator of serum triglyceride concentrations. An ApoA5 mouse nock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. Furthermore, peroxisome proliferators-activated receptoragonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. There is very little known about ApoA5 protein in human serum. Lately, it was established that ApoA5 is present in the human serum and it can be detected by polyclonal antibodies against both the HN2 and COOH termini, but at much lower concentration than other apolipoproteins.
ApoA5 是血清甘油三酯浓度的重要调节剂。 ApoA5 小鼠敲除模型产生的血清甘油三酯增加约四倍，而敲入模型人 ApoA5 产生的小鼠血清甘油三酯浓度降低了 50-70%。 此外，临床上用于降低血清甘油三酯浓度的过氧化物酶体增殖物激活的受体激动剂会导致 ApoA5 mRNA 表达增加。 关于人血清中的 ApoA5 蛋白知之甚少。 最近，已确定 ApoA5 存在于人血清中，并且可以通过针对 HN2 和 COOH 末端的多克隆抗体检测到，但浓度远低于其他载脂蛋白。

Description

APOA5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 366 amino acids and having a molecular mass of 41.3kDa.
APOA5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 APOA5 Human Recombinant 是单条非糖基化多肽链，含有 366 个氨基酸，分子量为 41.3kDa。
APOA5 在 N 端与 23 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

APOA5 protein solution contains 20mM Tris-HCl buffer , 10% glycerol and 0.4M Urea.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSRKGFWDY FSQTSGDKGR VEQIHQQKMA REPATLKDSL EQDLNNMNKF LEKLRPLSGS EAPRLPQDPV GMRRQLQEEL EEVKARLQPY MAEAHELVGW NLEGLRQQLK PYTMDLMEQV ALRVQELQEQ LRVVGEDTKA QLLGGVDEAW ALLQGLQSRV VHHTGRFKEL FHPYAESLVS GIGRHVQELH RSVAPHAPAS PARLSRCVQV LSRKLTLKAK ALHARIQQNL DQLREELSRA FAGTGTEEGA GPDPQMLSEE VRQRLQAFRQ DTYLQIAAFT RAIDQETEEV QQQLAPPPPG HSAFAPEFQQ TDSGKVLSKL QARLDDLWED ITHSLHDQGH SHLGDP.

Purity

Greater than 85.0% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • APOA5 Human, HEK

ProSpec-APOB Human/CYT-1042

ProSpec APOB Human,ProSpec CYT-1042,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,APOB, APO-B, Apolipoprotein B.起始材料供体已经过 HIV-1、HIV-2、HCV、HBSAG 抗体检测并证明为阴性。

APOB Human

Apolipoprotein-B Human
CYT-1042
ProSpec-APOB Human/CYT-1042 100µg
ProSpec-APOB Human/CYT-1042 1mg
ProSpec-APOB Human/CYT-1042 5mg

Catalogue number

CYT-1042

Synonyms

APOB, APO-B, Apolipoprotein B.

Introduction

ApoB is the main apolipoprotein of LDL, IDL, VLDL and chylomicrons particles that serves as the carrier of lipids , as well as cholesterol, in the water surrounding the cells within every tissue across the body. Though all the functional aspects of Apolipoprotein B within the LDL and other elements are considered to be rather uncertain, it serves as the key organizing protein of all other carriers of lipids. across LDL membranes, apolipoprotein B also serves as a ligand for LDL receptors in many cells across the body, meaning, it shows that lipid carriers that are set to cross into cells with Apolipoprotein B receptors, this is the way that lipids are transported within and into cells.

ApoB 是 LDL、IDL、VLDL 和乳糜微粒的主要载脂蛋白，在全身每个组织内的细胞周围的水中充当脂质和胆固醇的载体。 尽管 LDL 和其他元素中载脂蛋白 B 的所有功能方面都被认为是相当不确定的，但它是所有其他脂质载体的关键组织蛋白。 穿过 LDL 膜，载脂蛋白 B 还充当全身许多细胞中 LDL 受体的配体，这意味着，它表明脂质载体将进入带有载脂蛋白 B 受体的细胞，这是脂质在体内和 进入细胞。

Description

Human APOB produced from Human plasma having a molecular mass of 550 kDa.

Source

Human Plasma.

Physical Appearance

Sterile Filtered lyophilized powder.

Formulation

The protein was lyophilized from a 0.5mg/ml solution of 50mM Na2CO3 pH-10 containing 50mM NaCl and 10mM sodium deoxycholate.

从含有 50mM NaCl 和 10mM 脱氧胆酸钠的 50mM Na2CO3 pH-10 的 0.5mg/ml 溶液中冻干蛋白质。

Solubility

It is recommended to reconstitute the lyophilized APOB in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干 APOB，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized APOB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, APOB should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage, it is recommended to add a carrier protein . Please prevent freeze-thaw cycles.

冻干的 APOB 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，APOB 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。 请防止冻融循环。

Purity

Greater than 95.0%.

Human Virus Test

Starting material donor has been tested and certified negative for antibodies to HIV-1,HIV-2, HCV, HBSAG.

起始材料供体已经过 HIV-1、HIV-2、HCV、HBSAG 抗体检测并证明为阴性。

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Prospec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-APOC1 Human/CYT-812

ProSpec APOC1 Human,ProSpec CYT-812,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在大肠杆菌中产生的 APOC1 Human Recombinant 是一条含有 80 个氨基酸且分子量为 9.0kDa 的单一非糖基化多肽链。 APOC1 在 N 端与 23 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

APOC1 Human

Apolipoprotein C-I Human Recombinant
CYT-812
ProSpec-APOC1 Human/CYT-812 2µg
ProSpec-APOC1 Human/CYT-812 10µg
ProSpec-APOC1 Human/CYT-812 1mg

Catalogue number

CYT-812

Synonyms

Apolipoprotein C-I, Apo-CI, ApoC-I, Apolipoprotein C1, APOC1.

Introduction

Apolipoprotein C-I which expressed mainly in the liver is a part of the apolipoprotein C family. APOC1 which is usually found in plasma and responsible for the activation of esterified lechitin cholesterol takes an important part in the exchange of esterified cholesterol among lipoproteins and in removal of cholesterol from tissues. APOC1 is activated when monocytes differentiate into macrophages. APOC1 protein’s main role is to inhibit CETP by altering the electric charge of HDL molecules. APOC1 is also binds free fatty acids and reduces their intracellular esterification.
主要在肝脏中表达的载脂蛋白C-I是载脂蛋白C家族的一部分。 APOC1 通常存在于血浆中，负责激活酯化卵磷脂胆固醇，在脂蛋白之间的酯化胆固醇交换和从组织中去除胆固醇中起重要作用。 当单核细胞分化为巨噬细胞时，APOC1 被激活。 APOC1蛋白的主要作用是通过改变HDL分子的电荷来抑制CETP。 APOC1 还结合游离脂肪酸并减少它们的细胞内酯化。

Description

APOC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 80 amino acids and having a molecular mass of 9.0kDa. APOC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 APOC1 Human Recombinant 是一条含有 80 个氨基酸且分子量为 9.0kDa 的单一非糖基化多肽链。 APOC1 在 N 端与 23 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

APOC1 protein solution contains 20mM Tris-HCl buffer , 0.15M NaCl and 10% glycerol.
APOC1 蛋白溶液含有 20mM Tris-HCl 缓冲液、0.15M NaCl 和 10% 甘油。

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSTPDVSSA LDKLKEFGNT LEDKARELIS RIKQSELSAK MREWFSETFQ KVKEKLKIDS.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-APOD Human, HEK/CYT-768

ProSpec APOD Human HEK,ProSpec CYT-768,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,在 HEK 细胞中产生的载脂蛋白-D 人重组体是一条单一的糖基化多肽链，总共包含 175 个氨基酸，分子量为 20.1kDa，并在 C 末端与 6 个氨基酸的 His 标签融合。

APOD Human, HEK

Apolipoprotein-D Human Recombinant, HEK
CYT-768
ProSpec-APOD Human, HEK/CYT-768 2µg
ProSpec-APOD Human, HEK/CYT-768 10µg
ProSpec-APOD Human, HEK/CYT-768 1mg

Catalogue number

CYT-768

Synonyms

Apolipoprotein D, Apo-D, ApoD.

Introduction

Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, Apolipoprotein-D is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid.
Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimer’s disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from 1 cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue.
载脂蛋白-D主要与人血浆中的高密度脂蛋白有关。载脂蛋白-D 是一种非典型载脂蛋白，根据其一级结构，载脂蛋白-D 是载脂蛋白家族的成员。脂质运载蛋白采用β-桶三级结构并运输小的疏水配体。载脂蛋白-D 结合胆固醇、孕酮、孕烯醇酮、胆红素和花生四烯酸。
载脂蛋白-D在脾脏、睾丸和脑中具有高水平表达的许多组织中表达。载脂蛋白-D 以高浓度存在于患有严重乳腺囊性疾病的女性的囊液中，这种疾病与乳腺癌风险增加有关。载脂蛋白-D 积聚在再生的周围神经和患有神经退行性疾病（如阿尔茨海默病）患者的脑脊液中。载脂蛋白-D 参与中枢和外周神经系统的维护和修复。载脂蛋白-D 是一种多配体、多功能转运蛋白，可将配体从一个细胞转运到器官内的另一个细胞，清除器官内的配体以转运到血液中，或者可以将配体从循环转运到器官内的特定细胞组织。

Description

Apolipoprotein-D Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain containing a total of 175 amino acids, having a molecular mass of 20.1kDa and fused to a 6 aa His tag at C-Terminus.
The Human APOD is purified by proprietary chromatographic techniques.
在 HEK 细胞中产生的载脂蛋白-D 人重组体是一条单一的糖基化多肽链，总共包含 175 个氨基酸，分子量为 20.1kDa，并在 C 末端与 6 个氨基酸的 His 标签融合。
Human APOD 通过专有的色谱技术进行纯化。

Source

HEK 293.

Physical Appearance

Filtered White lyophilized powder.

Formulation

Filtered and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.

Solubility

It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

建议加入去离子水至工作浓度0.5mg/ml，让冻干颗粒完全溶解。 产品不是无菌的！ 在细胞培养中使用之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间。

Purity

Greater than 95.0% as determined by -PAGE.

Amino acid sequence

QAFHLGKCPN PPVQENFDVN KYLGRWYEIE KIPTTFENGR CIQANYSLME NGKIKVLNQE LRADGTVNQI EGEATPVNLT EPAKLEVKFS WFMPSAPYWI LATDYENYAL VYSCTCIIQL FHVDFAWILA RNPNLPPETV DSLKNILTSN NIDVKKMTVT DQVNCPKLSH HHHHH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • APO D Human, GST
  • Apo D Human

ProSpec-APOE3 Human/CYT-874

ProSpec APOE3 Human,ProSpec CYT-874,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Apolipoprotein E, LDLCQ5, APO-E, LPG, AD2, Alzheimer Disease 2 , Apolipoprotein E3, APOE.

APOE3 Human

Apolipoprotein E3 Human Recombinant
CYT-874
ProSpec-APOE3 Human/CYT-874 100µg
ProSpec-APOE3 Human/CYT-874 500µg
ProSpec-APOE3 Human/CYT-874 1mg

Catalogue number

CYT-874

Synonyms

Apolipoprotein E, LDLCQ5, APO-E, LPG, AD2, Alzheimer Disease 2 , Apolipoprotein E3, APOE.
载脂蛋白 E、LDL5、APO-E、LPG、AD2、Algeimer Disase 2、载脂蛋白 E、APOE。

Introduction

Apolipoprotein E3 is a 34kDa protein component of serum chylomicrons, VLDL, and HDL particles. ApoE mediates the binding, uptake, and catabolism of these particles as a result of interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is imperative in fatty acid homeostasis and memory formation. Polymorphisms encode 3 variants , which are differentially connected to the development of atherosclerosis and neurogenerative disorders, mainly Alzheimer’s disease.
载脂蛋白 E3 是血清乳糜微粒、VLDL 和 HDL 颗粒的 34kDa 蛋白质成分。 由于与肝脏和大脑中的 ApoE 受体和 LDL 受体相互作用，ApoE 介导这些颗粒的结合、摄取和分解代谢。 ApoE 在脂肪酸稳态和记忆形成中必不可少。 多态性编码 3 个变体，它们与动脉粥样硬化和神经生成疾病（主要是阿尔茨海默病）的发展有不同的联系。

Description

APOE3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 306 amino acids and having a molecular mass of 35.2kDa.

在大肠杆菌中产生的 APOE3 Human Recombinant 是一条单一的、非糖基化的多肽链，包含 306 个氨基酸，分子量为 35.2kDa。

APOE 在 N 端与一个 Met 和一个 6 个氨基酸的 His 标签 [M-HHHHHH] 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

sterile filtered solution supplied in 10mM MOPS, 50mM NaCl, 0.2 % CHAPS and 1mM TCEP, PH 7.5.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein . Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。对于长期储存，建议添加载体蛋白。 避免多次冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Amino acid sequence

MHHHHHHKVE QAVETEPEPE LRQQTEWQSG QRWELALGRF WDYLRWVQTL SEQVQEELLS SQVTQELRAL MDETMKELKA YKSELEEQLT PVAEETRARL SKELQAAQAR LGADMEDVCG RLVQYRGEVQ AMLGQSTEEL RVRLASHLRK LRKRLLRDAD DLQKRLAVYQ AGAREGAERG LSAIRERLGP LVEQGRVRAA TVGSLAGQPL QERAQAWGER LRARMEEMGS RTRDRLDEVK EQVAEVRAKL EEQAQQIRLQ AEAFQARLKS WFEPLVEDMQ RQWAGLVEKV QAAVGTSAAP VPSDNH.

Biological Activity

Immobilized rHuApoE3 binds to rMuVLDLR with EC50 less than 0.075-0.375µg/ml.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

  • APOE4 Human

ProSpec-APOE4 Human/CYT-968,APOE3 Human

ProSpec APOE4 Human,ProSpec CYT-968,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,载脂蛋白 E 是一种乳糜微粒脂蛋白，对脂蛋白的代谢和脂质转运至关重要。 APOE基因有3个等位基因，分别命名为APOE2、APOE3和APOE4。

APOE4 Human

Apolipoprotein E4 Human Recombinant
CYT-968
ProSpec-APOE4 Human/CYT-968 100µg
ProSpec-APOE4 Human/CYT-968 500µg
ProSpec-APOE4 Human/CYT-968 1mg

Catalogue number

CYT-968

Synonyms

Apolipoprotein E, APO-E, Alzheimer Disease 2 , Apolipoprotein E3, LDLCQ5, LPG, AD2, APOE.

Introduction

Apolipoprotein E is a chylomicron lipoprotein which is essential for the metabolism of lipoproteins and lipid transport. The APOE gene has 3 alleles, designated APOE2, APOE3, and APOE4. The APOE allelic proteins differ by only one or two amino acids, however have different biological structures and functions. APOE3 is the most common and neutral allele. The APOE4 allele is linked with an increased risk for Alzheimer’s Disease and coronary artery disease . The APOE4 protein morphology decreases the ability of APOE4 to clear beta-amyloid protein from the brain, resulting in AD progression. The APOE2 allele is linked with type III hyperlipoproteinemia, which is characterized by defects in the clearance of plasma lipoproteins, however APOE2 may have a protective effect against AD.
载脂蛋白 E 是一种乳糜微粒脂蛋白，对脂蛋白的代谢和脂质转运至关重要。 APOE基因有3个等位基因，分别命名为APOE2、APOE3和APOE4。 APOE等位基因蛋白只有一个或两个氨基酸不同，但具有不同的生物学结构和功能。 APOE3 是最常见和中性的等位基因。 APOE4 等位基因与阿尔茨海默病和冠状动脉疾病的风险增加有关。 APOE4 蛋白形态降低了 APOE4 从大脑中清除 β-淀粉样蛋白的能力，导致 AD 进展。 APOE2等位基因与III型高脂蛋白血症相关，其特征是血浆脂蛋白清除缺陷，但APOE2可能对AD具有保护作用。

Description

Apolipoprotein E4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing a total of 301 amino acids and having a molecular mass of 34.4kDa. The # is P02649 VAR_000652.

在大肠杆菌中产生的载脂蛋白 E4 Human Recombinant 是一条单一的、非糖基化的多肽链，总共含有 301 个氨基酸，分子量为 34.4kDa。 # 是 P02649 VAR_000652。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a sterile filtered aqueous solution containing 10mM sodium phosphate, 0.5 mM DTT, pH 7.5.

从含有 10 mM 磷酸钠、0.5 mM DTT、pH 7.5 的无菌过滤水溶液冻干。

Solubility

It is recommended to reconstitute the lyophilized APOE4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 APOE4，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized APOE4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APOE4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 APOE4 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，APOE4 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 90% as determined by -PAGE.

Amino acid sequence

GPKVE QAVETEPEPE LRQQTEWQSG QRWELALGRF WDYLRWVQTL SEQVQEELLS SQVTQELRAL MDETMKELKA YKSELEEQLT PVAEETRARL SKELQAAQAR LGADMEDVRG RLVQYRGEVQ AMLGQSTEEL RVRLASHLRK LRKRLLRDAD DLQKRLAVYQ AGAREGAERG LSAIRERLGP LVEQGRVRAA TVGSLAGQPL QERAQAWGER LRARMEEMGS RTRDRLDEVK EQVAEVRAKL EEQAQQIRLQ AEAFQARLKS WFEPLVEDMQ RQWAGLVEKV QAAVGTSAAP VPSDNH.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

  • APOE3 Human

ProSpec-APOH/CYT-189

ProSpec APOH,ProSpec APOH CYT-189,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,载脂蛋白 H 附着在多种带负电荷的物质上，例如磷脂和硫酸葡聚糖。 APOH 通过附着在受损细胞表面的磷脂上来防止内在凝血级联反应的激活。 APOH由肝脏表达并分泌到血浆中。

APOH

Apolipoprotein-H Human Recombinant
CYT-189
ProSpec-APOH/CYT-189 2µg
ProSpec-APOH/CYT-189 10µg
ProSpec-APOH/CYT-189 1mg

Catalogue number

CYT-189

Synonyms

Beta-2-glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Apolipoprotein H, Apo-H, Beta-2-glycoprotein I, B2GPI, BetaGPI, APOH, B2G1, BG, B2GP1.

Introduction

Apolipoprotein H attaches to numerous kinds of negatively charged substances for instance phospholipids, and dextran sulfate. APOH prevents activation of the intrinsic blood coagulation cascade by attaching to phospholipids on the surface of damaged cells. APOH expressed by the liver and secreted in the plasma.

载脂蛋白 H 附着在多种带负电荷的物质上，例如磷脂和硫酸葡聚糖。 APOH 通过附着在受损细胞表面的磷脂上来防止内在凝血级联反应的激活。 APOH由肝脏表达并分泌到血浆中。

Description

APOH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 349 amino acids and having a molecular mass of 38.6kDa.
APOH is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 APOH Human Recombinant 是一个单一的、非糖基化的多肽链，包含 349 个氨基酸，分子量为 38.6kDa。
APOH 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

APOH protein solution contains 20mM Tris-HCl buffer , 2M urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSGRTCPKP DDLPFSTVVP LKTFYEPGEE ITYSCKPGYV SRGGMRKFIC PLTGLWPINT LKCTPRVCPF AGILENGAVR YTTFEYPNTI SFSCNTGFYL NGADSAKCTE EGKWSPELPV CAPIICPPPS IPTFATLRVY KPSAGNNSLY RDTAVFECLP QHAMFGNDTI TCTTHGNWTK LPECREVKCP FPSRPDNGFV NYPAKPTLYY KDKATFGCHD GYSLDGPEEI ECTKLGNWSA MPSCKASCKV PVKKATVVYQ GERVKIQEKF KNGMLHGDKV SFFCKNKEKK CSYTEDAQCI DGTIEVPKCF KEHSSLAFWK TDASDVKPC.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

Related Products

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  • APOH Bovine
  • APOH Human, sf9

ProSpec-APOH Bovine/PRO-1504,APOH

ProSpec APOH Bovine,ProSpec APOH Bovine PRO-1504,ProSpec APOH,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,Beta-2-glycoprotein 1, Apolipoprotein H, Apo-H, Beta-2-glycoprotein I, B2GPI, BetaGPI, APOH.

APOH Bovine

Apoliporotein-H Bovine
PRO-1504
ProSpec-APOH Bovine/PRO-1504 10µg
ProSpec-APOH Bovine/PRO-1504 50µg
ProSpec-APOH Bovine/PRO-1504 1mg

Catalogue number

PRO-1504

Synonyms

Beta-2-glycoprotein 1, Apolipoprotein H, Apo-H, Beta-2-glycoprotein I, B2GPI, BetaGPI, APOH.

Introduction

Apolipoprotein H attaches to numerous kinds of negatively charged substances for instance phospholipids, and dextran sulfate. APOH prevents activation of the intrinsic blood coagulation cascade by attaching to phospholipids on the surface of damaged cells. APOH expressed by the liver and secreted in the plasma.

载脂蛋白 H 附着在多种带负电荷的物质上，例如磷脂和硫酸葡聚糖。 APOH 通过附着在受损细胞表面的磷脂上来防止内在凝血级联反应的激活。 APOH由肝脏表达并分泌到血浆中。

Description

Bovine Apolipoprotein-H polypeptide is purified from fetal calf serum by proprietary protein-chemical techniques, having an Mw of approximately 70kDa.

Source

Fetal calf serum .

Physical Appearance

Sterile Filtered clear solution.

Formulation

APOH protein solution is supplied in 16mM Tris-HCl pH-8.0, 280mM NaCl, 0.16mM AEBSF and 20% glycerol.

APOH 蛋白溶液以 16mM Tris-HCl pH-8.0、280mM NaCl、0.16mM AEBSF 和 20% 甘油的形式提供。

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
避免多次冻融循环。

Purity

Greater than 70% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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  • APOH

ProSpec-APOH Human, sf9/PRO-388

ProSpec APOH Human sf9,ProSpec PRO-388,ProSpec APOH Bovine,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,SF9 中产生的载脂蛋白-H 人重组体是一种糖基化的多肽链，含有 326 个氨基酸，分子量为 38,200 道尔顿，总质量为 45kDa。 APOH 用 -6xHis 标签表达，并通过专有色谱技术进行纯化。

APOH Human, sf9

Apolipoprotein-H Human Recombinant, Sf9
PRO-388
ProSpec-APOH Human, sf9/PRO-388 5µg
ProSpec-APOH Human, sf9/PRO-388 20µg
ProSpec-APOH Human, sf9/PRO-388 1mg

Catalogue number

PRO-388

Synonyms

Beta-2-glycoprotein 1, Beta-2-glycoprotein I, Apolipoprotein H, Apo-H, B2GPI, BetaGPI, Activated protein C-binding protein, APC inhibitor, Anticardiolipin cofactor, APOH, B2G1, BG, B2GP-1, B2 Glycoprotein-I.
Beta-2-糖蛋白 1、Beta-2-糖蛋白 I、载脂蛋白 H、Apo-H、B2GPI、BetaGPI、活化蛋白 C 结合蛋白、APC 抑制剂、抗心磷脂辅因子、APOH、B2G1、BG、B2GP-1、B2 糖蛋白 -一世。

Introduction

Apolipoprotein H attaches to numerous kinds of negatively charged substances for instance heparin, phospholipids, and dextran sulfate. APOH prevents activation of the intrinsic blood coagulation cascade by attaching to phospholipids on the surface of damaged cells. APOH expressed by the liver and secreted in the plasma.
载脂蛋白 H 附着在多种带负电荷的物质上，例如肝素、磷脂和硫酸葡聚糖。 APOH 通过附着在受损细胞表面的磷脂上来防止内在凝血级联反应的激活。 APOH由肝脏表达并分泌到血浆中。

Description

Apolipoprotein-H Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 326 amino acids and having a molecular mass of 38,200 Dalton , 45kDa total mass. APOH is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
SF9 中产生的载脂蛋白-H 人重组体是一种糖基化的多肽链，含有 326 个氨基酸，分子量为 38,200 道尔顿，总质量为 45kDa。 APOH 用 -6xHis 标签表达，并通过专有色谱技术进行纯化。

Source

Sf9 insect cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

APOH is supplied in 16mM HEPES buffer pH-7.2, 200mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
避免多次冻融循环。

Purity

Greater than 95% as determined by -PAGE.

Usage

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-APOL4 Human/CYT-785

ProSpec-APOL4 Human/CYT-785,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,APOL-IV, APOLIV, Apolipoprotein L4, ApoL-IV.

APOL4 Human

Apolipoprotein L 4 Human Recombinant
CYT-785
ProSpec-APOL4 Human/CYT-785 2µg
ProSpec-APOL4 Human/CYT-785 10µg
ProSpec-APOL4 Human/CYT-785 100µg

Catalogue number

CYT-785

Synonyms

APOL-IV, APOLIV, Apolipoprotein L4, ApoL-IV.

Introduction

Apolipoprotein L 4 belongs to the apolipoprotein L family. APOL4 plays a role in lipid exchange and transport through the body, in addition to reverse cholesterol transport from peripheral cells to the liver. Two transcript variants encoding two different isoforms have been found for this gene. APOL4 is highly expressed in spinal cord, placenta, adrenal gland; also detected in spleen, bone marrow, uterus, trachea, mammary gland and testis
载脂蛋白 L 4 属于载脂蛋白 L 家族。 APOL4 在脂质交换和通过身体的运输中发挥作用，此外还可以逆转胆固醇从外周细胞到肝脏的运输。 已经为该基因发现了编码两种不同同种型的两种转录变体。 APOL4在脊髓、胎盘、肾上腺中高度表达； 还在脾脏、骨髓、子宫、气管、乳腺和睾丸中检测到

Description

APOH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 371 amino acids and having a molecular mass of 41.1kDa.
APOH is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 APOH Human Recombinant 是一个单一的、非糖基化的多肽链，包含 371 个氨基酸，分子量为 41.1kDa。
APOH 在 N 末端与 23 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

APOH protein solution contains 20mM Tris-HCl buffer, , 10% glycerol and 0.4M Urea.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.
如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Purity

Greater than 85.0% as determined by -PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMGSWVQL ITSVGVQQNH PGWTVAGQFQ EKKRFTEEVI EYFQKKVSPV HLKILLTSDE AWKRFVRVAE LPREEADALY EALKNLTPYV AIEDKDMQQK EQQFREWFLK EFPQIRWKIQ ESIERLRVIA NEIEKVHRGC VIANVVSGST GILSVIGVML APFTAGLSLS ITAAGVGLGI ASATAGIASS IVENTYTRSA ELTASRLTAT STDQLEALRD ILRDITPNVL SFALDFDEAT KMIANDVHTL RRSKATVGRP LIAWRYVPIN VVETLRTRGA PTRIVRKVAR NLGKATSGVL VVLDVVNLVQ DSLDLHKGAK SESAESLRQW AQELEENLNE LTHIHQSLKA G.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

ProSpec-APOM Human/CYT-715

ProSpec APOM Human,ProSpec CYT-715,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,APOM 属于脂质运载蛋白家族，与高密度脂蛋白有关，在较小程度上与低密度脂蛋白和富含甘油三酯的脂蛋白有关。 APOM 通过质膜分泌，但仍与膜结合，参与脂质转运。

APOM Human

Apolipoprotein-M Human Recombinant
CYT-715
ProSpec-APOM Human/CYT-715 2µg
ProSpec-APOM Human/CYT-715 10µg
ProSpec-APOM Human/CYT-715 1mg

Catalogue number

CYT-715

Synonyms

G3a, HSPC336, NG20, Apolipoprotein M, APOM, Apo-M, MGC22400.

Introduction

APOM is belongs to the lipocalin protein family and is associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. APOM is secreted through the plasma membrane but remains membrane-bound, where it takes part in lipid transport.
APOM 属于脂质运载蛋白家族，与高密度脂蛋白有关，在较小程度上与低密度脂蛋白和富含甘油三酯的脂蛋白有关。 APOM 通过质膜分泌，但仍与膜结合，参与脂质转运。

Description

APOM Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 187 amino acids and having a molecular mass of 20.9 kDa. APOM protein is fused to a 21 amino acid His-Tag at N-terminus and purified by standard chromatography.
在大肠杆菌中产生的 APOM Human Recombinant 是一种单一的、非糖基化的多肽链，含有 187 个氨基酸，分子量为 20.9 kDa。 APOM 蛋白在 N 端与 21 个氨基酸的 His-Tag 融合，并通过标准色谱法纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

APOM Human solution containing 20mM Tris-HCl pH-8 1mM DTT & 10% glycerol.
APOM 人溶液含有 20mM Tris-HCl pH-8 1mM DTT 和 10% 甘油。

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MCPEHSQLTT LGVDGKEFPE VHLGQWYFIA GAAPTKEELA TFDPVDNIVF NMAAGSAPMQ LHLRATIRMK DGLCVPRKWI YHLTEGSTDL RTEGRPDMKT ELFSSSCPGG IMLNETGQGY QRFLLYNRSP HPPEKCVEEF KSLTSCLDSK AFLLTPRNQE ACELSNN.

Purity

Greater than 90% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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ProSpec-APOM Human, HEK/CYT-026

ProSpec APOM Human HEK,ProSpec CYT-026,ProSpec产品,ProSpec代理,ProSpec产品目录,ProSpec 细胞因子,ProSpec Cytokines,G3a, HSPC336, NG20, Apolipoprotein M, APOM, Apo-M, MGC22400.

APOM Human, HEK

Apolipoprotein-M Human Recombinant, HEK
CYT-026
ProSpec-APOM Human, HEK/CYT-026 2µg
ProSpec-APOM Human, HEK/CYT-026 10µg
ProSpec-APOM Human, HEK/CYT-026 1mg

Catalogue number

CYT-026

Synonyms

G3a, HSPC336, NG20, Apolipoprotein M, APOM, Apo-M, MGC22400.

Introduction

APOM is belongs to the lipocalin protein family and is associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. APOM is secreted through the plasma membrane but remains membrane-bound, where it takes part in lipid transport.
APOM 属于脂质运载蛋白家族，与高密度脂蛋白有关，在较小程度上与低密度脂蛋白和富含甘油三酯的脂蛋白有关。 APOM 通过质膜分泌，但仍与膜结合，参与脂质转运。

Description

APOM HEK Human Recombinant Protein is 20 kDa protein containing 179 amino acid residues of the APOM Human and 13 additional amino acid residues of flag Tag.
APOM HEK Human Recombinant Protein 是一种 20 kDa 的蛋白质，含有 APOM Human 的 179 个氨基酸残基和 flag Tag 的 13 个附加氨基酸残基。

Source

HEK293.

Physical Appearance

White lyophilized powder.

Formulation

APOM Human was filtered and lyophilized from 0.5 mg/ml supplied in 20mM TRIS and 50mM NaCl, pH 7.5.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。 产品不是无菌的！ 在用于细胞培养之前，请用适当的无菌过滤器过滤产品。

Stability

Store lyophilized APOM Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted APOM Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
将冻干的 APOM Human 储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重构的 APOM Human 可以在 4°C 下储存一段有限的时间； 在 4°C 下两周后它没有显示任何变化。

Amino acid sequence

HVDYKDDDDK PAGCPEHSQL TTLGVDGKEF PEVHLGQWYF IAGAAPTKEE LATFDPVDNI VFNMAAGSAP MQLHLRATIR MKDGLCVPRK WIYHLTEGST DLRTEGRPDM KTELFSSSCP GGIMLNETGQ GYQRFLLYNR SPHPPEKCVE EFKSLTSCLD SKAFLLTPRN QEACELSNN

Applications

Cell culture and/or animal studies, ELISA and Western blotting.

Purity

Greater than 90% as determined by -PAGE.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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