- Products
- DNA
- Phosphoramidites
- Abasic phosphoramidite
Abasic phosphoramidite
| Cat. # | Quantity | Price | Lead time | |
|---|---|---|---|---|
| 3009-1g | 1 g | 990.00$
|
Cleavage of the N-glycosyl bond between a base and its 2-deoxyribose moiety in DNA generates an apurinic/apyrimidinic (so-called abasic) site. This phenomenon, which is referred to as depurination or depyrimidination occurs spontaneously under physiological conditions.
Phosphoramidites are modified nucleosides that are used in DNA and oligonucleotide synthesis. Abasic phosphoramidite replicates the loss of base pairing ability by a nucleotide. This modification can be useful when examining DNA damage and repair.
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Alkyne-PEG4-phosphoramidite
Alkyne phosphoramidite for conjugation of oligonucleotides with biomolecules.
Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.
lumiprobe -endo-BCN CE-Phosphoramidite
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- Click chemistry
- endo-BCN CE-Phosphoramidite
endo-BCN CE-Phosphoramidite
| Cat. # | Quantity | Price | Lead time | |
|---|---|---|---|---|
| 3005-100mg | 100 mg | –  Sample request |
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| 3005-250mg | 250 mg | 800.00$
|
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| 3005-1g | 1 g | 2350.00$
|
Bicyclononyne (BCN) is a stable and one of the most reactive cyclooctynes for Copper-free click chemistry. Unlike dibenzocyclooctyne (DBCO), BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA). Being an endo-stereoisomer, Bicyclononine in endo-BCN CE-phosphoramidite provides a higher rate of cycloaddition compared to its exo-conformer.
BCN-labeled oligonucleotides may be used for the conjugation to azide- or tetrazine-containing solid surfaces, polymers, and large proteins.
Notes:
Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.
Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1).
Customers also purchased with this product
Cyanine5 azide
Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.
Oligo synthesis details
| Diluent: | Anhydrous Acetonitrile |
| Deprotection conditions: | identical to protected nucleobases |
lumiprobe -VIC phosphoramidite, 6-isomer
- Products
- DNA
- Phosphoramidites
- VIC phosphoramidite, 6-isomer
VIC phosphoramidite, 6-isomer
| Cat. # | Quantity | Price | Lead time | |
|---|---|---|---|---|
| DH260 | 250 mg | 490.00$
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| FH260 | 1 g | 990.00$
|
VIC is an asymmetrical xanthene dye (two flanking rings are not the same). This dye is used for the design of qPCR probes. The spectral properties of VIC are similar to HEX and JOE.
This phosphoramidite reagent allows to introduce the label onto 5′-terminus of the oligonucleotide.
Customers also purchased with this product
JOE phosphoramidite, 6-isomer
JOE dye phosphoramidite for synthesis of labeled oligonucleotides. JOE absorption and emission maxima are between maxima of FAM and TAMRA/ROX dyes.
HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.
TET phosphoramidite for synthesis of 5’-labeled oligonucleotides.
Oligo synthesis details
| Diluent: | acetonitrile |
dU (Deoxyuridine) phosphoramidite
dU (Deoxyuridine) phosphoramidite,lumiprobe代理,lumiprobe产品
- Products
- DNA
- Phosphoramidites
- dU (Deoxyuridine) phosphoramidite
dU (Deoxyuridine) phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 320-1g | 1 g | – | 10 days |
| 320-10g | 10 g | 250.00$ | 21 days |
| 320-50g | 50 g | please inquire | 21 days |
dU (Deoxyuridine) phosphoramidite相关产品
FAM-dT phosphoramidite
Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.
亚磷酰胺用于合成在序列中间用荧光素 (FAM) 标记的寡核苷酸。
Alkyne dT phosphoramidite
Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.
亚磷酰胺修饰剂，用于合成在链中间含有炔烃修饰的寡核苷酸。
Hexaethylene glycol phosphoramidite
Spacer phosphoramidite 18 (hexaethylene glycol amidite) provides a long hydrophilic linker for oligo synthesis.
间隔亚磷酰胺 18（六甘醇酰胺）为寡核苷酸合成提供了一个长的亲水接头。
General properties
| Appearance: | pale yellow solid foam |
| Molecular weight: | 730.79 |
| CAS number: | 109389-30-2 |
| Molecular formula: | C39H47N4O8P |
| IUPAC name: | 5′-O-(4,4′-Dimethoxytrityl)-2′-deoxyuridine-3′-O-[O-(2-cyanoethyl)-N,N’-diisopropylphosphoramidite] |
| Solubility: | good in acetonitrile, DCM |
| Quality control: | NMR 1H, NMR 31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 干燥。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
Алкин-ПЭГ4-фосфорамидит
Алкин-ПЭГ4-фосфорамидит,包含在亚磷酰胺结构中的四甘醇接头提供了增加的亲水性，并且还降低了合成缀合物的毒性和免疫原性。
- Products
- DNA
- Phosphoramidites
- Алкин-ПЭГ4-фосфорамидит
Алкин-ПЭГ4-фосфорамидит
| Cat. # | Quantity | Price | Lead time |
|---|---|---|---|
| 2779-100mg | 100 mg | 390.00$ | 21 days |
| 2779-250mg | 250 mg | 680.00$ | 21 days |
| 2779-1g | 1 g | 1640.00$ | 21 days |
| 2779-5g | 5 g | please inquire | 21 days |
Алкин-ПЭГ4-фосфорамидит — представляет собой фосфорамидит на основе тетраэтиленгликоля с терминальной тройной связью и может использоваться для синтеза различных функционализированных олигонуклеотидов. Алкиновый фрагмент в синтезированных молекулах вступает в реакции Click Chemistry с соединениями, содержащими азидную группу в присутствии солей меди(I), приводя к различным олигонуклеотидным конъюгатам, например с красителями, пептидами или гликанами. Выход полученных подобным образом соединений довольно высок, а выделение и очистка продуктов зачастую проще по сравнению с молекулами, полученные в результате взаимодействия амино-модифицированных олигонуклеотидов с активированными эфирами, например, красителей. Важной характеристикой Click Chemistry реакций является их биоортогональность, поскольку ни терминальные алкиновые, ни азидные функциональные группы обычно не присутствуют в биомолекулах.
Тетраэтиленгликольный линкер, входящий в структуру фосфорамидита, обеспечивает повышенную гидрофильность, а также снижает токсичность и иммуногенность синтезированных конъюгатов.
炔烃-PEG4-亚磷酰胺是一种基于四甘醇的亚磷酰胺，具有末端三键，可用于合成各种功能化的寡核苷酸。合成分子中的炔烃片段在铜 (I) 盐存在下与含有叠氮基的化合物进入 Click Chemistry 反应，从而产生各种寡核苷酸缀合物，例如与染料、肽或聚糖。以这种方式获得的化合物的产率非常高，与由氨基修饰的寡核苷酸与活化的酯（例如染料）相互作用获得的分子相比，产物的分离和纯化通常更简单。 Click Chemistry 反应的一个重要特征是它们的生物正交性，因为末端炔烃和叠氮官能团通常不存在于生物分子中。
包含在亚磷酰胺结构中的四甘醇接头提供了增加的亲水性，并且还降低了合成缀合物的毒性和免疫原性。
Общие свойства
| Вид продукта: | желтое масло |
| Молекулярная масса: | 432.49 |
| CAS-номер: | 1682657-14-2 |
| Брутто-формула: | C20H37N2O6P |
| Название IUPAC: | 2-cyanoethyl (3,6,9,12-tetraoxapentadec-14-yn-1-yl) diisopropylphosphoramidite |
| Растворимость: | хорошая в ацетонитриле и дихлорметане |
| Контроль качества: | ЯМР 1H и 31P, ВЭЖХ-МС (95%) |
| Условия хранения: | Хранение: 12 месяцев (с момента доставки) при -20°C в темноте. Транспортировка: до трех недель при комнатной температуре. Берегите от влаги. |
| MSDS: | Download |
| Product specifications |
Олигонуклеотидный синтез
| Растворитель: | сухой ацетонитрил (растворить до концентрации 0.1 М, хранение – 1 неделя). |
| Условия конденсации: | Стандартное, как для фосфорамидитов природных нуклеозидов. |
| Условия деблокирования: | идентичные защищенным природным нуклеотидам |
TFA-aminolinker C6 phosphoramidite
TFA-aminolinker C6 phosphoramidite,5′-terminal amino modified oligos are used for the production of DNA arrays, and for subsequent 5′-terminal modification with NHS esters.
- Products
- DNA
- Phosphoramidites
- TFA-aminolinker C6 phosphoramidite
TFA-aminolinker C6 phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 1777-100mg | 100 mg | – | 21 days |
| 1777-250mg | 250 mg | 110.00$ | 21 days |
| 1777-1g | 1 g | 190.00$ | 21 days |
| 1777-5g | 5 g | 590.00$ | 21 days |
| 1777-10g | 10 g | please inquire | 21 days |
N-trifluoroacetyl (TFA) protected aminolink C6 phosphoramidite for the synthesis of 5′-amino-modified oligonucleotides. TFA protection is smoothly removed during ammonia deprotection, and does not require any additional steps. If necessary, the resulting oligonucleotides can be purified by ion exchange chromatography, or gel electrophoresis.
5′-terminal amino modified oligos are used for the production of DNA arrays, and for subsequent 5′-terminal modification with NHS esters.
N-三氟乙酰 (TFA) 保护氨基连接 C6 亚磷酰胺，用于合成 5′-氨基修饰的寡核苷酸。 TFA 保护在氨脱保护过程中被顺利去除，不需要任何额外的步骤。 如果需要，可以通过离子交换色谱法或凝胶电泳法纯化得到的寡核苷酸。
5′-末端氨基修饰寡核苷酸用于生产 DNA 阵列，以及随后用 NHS 酯进行 5′-末端修饰。
TFA-aminolinker C6 phosphoramidite相关产品
ROX azide, 5- isomer
ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.
ROX（又名罗丹明 X 或罗丹明 101）叠氮化物，用于通过 Click Chemistry 进行生物分子标记。
DMS(O)MT aminolink C6
An amidite for the introduction of amino group.
用于引入氨基的亚酰胺。
BDP FL NHS ester
Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
氨基反应性 BDP FL，一种非常明亮且光稳定的荧光素通道染料。
General properties
| Appearance: | colorless to yellowish liquid |
| Molecular weight: | 413.42 |
| CAS number: | 133975-85-6 |
| Molecular formula: | C17H31N3F3O3P |
| Solubility: | good in acetonitrile, THF, DCM |
| Quality control: | NMR 1H, NMR 31P, functional testing |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 干燥。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
TET phosphoramidite, 6-isomer
TET phosphoramidite, 6-isomer,TET 亚磷酰胺用于合成荧光标记的寡核苷酸，纯 6 异构体。
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- DNA
- Phosphoramidites
- TET phosphoramidite, 6-isomer
TET phosphoramidite, 6-isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| C8160 | 100 mg | – | 10 days |
| D8160 | 250 mg | 340.00$ | in stock |
| F8160 | 1 g | 990.00$ | in stock |
| G8160 | 5 g | 3900.00$ | 10 days |
| H8160 | 10 g | please inquire | 10 days |
TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.
TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).
TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).
5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via RCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM® 310 Genetic Analyzer.
TET 亚磷酰胺用于合成荧光标记的寡核苷酸，纯 6 异构体。
TET（四氯荧光素）是一种绿色荧光荧光素衍生物（最大吸收波长为 519 nm，最大发射波长为 535 nm）。
TET 亚磷酰胺用于合成荧光标记的引物和用于 qPCR 的杂交探针。 TET 可与 DusQ1 荧光猝灭剂一起使用（可与 500 Å DusQ1 CPG 500 一起使用）。
5′ 标记的引物与未标记的反向引物一起使用，通过 RCR 进行微卫星扩增，然后进行片段分析。 TET 标记的扩增产物可以使用各种毛细管电泳测序仪进行分析，包括 ABI PRISM® 310 遗传分析仪。
Recommendations for using the reagent:
使用试剂的建议：
Condensation: 3 min.
Deprotection: standard conditions with 25% aqueous ammonium solution; deprotection time depends on nucleic base profile and their protective properties (deprotection for 17 hours at 55°C removes all protection groups from standard nucleic acids). AMA (1:1 mixture of concentrated aqueous ammonium/40% aqueous methylamine) can be used with ~5% non-fluorescent by-product forming. To avoid formation of the by-product, start deprotection with ammonium (30 min at room temperature), then add an equivalent volume of 40% aqueous methylamine solution and continue deprotection as usual with AMA (10 min at 65°C).
冷凝：3 分钟。
脱保护：标准条件用25%铵水溶液； 脱保护时间取决于核酸碱基谱及其保护特性（在 55°C 脱保护 17 小时会去除标准核酸中的所有保护基团）。 AMA（浓铵水溶液/40% 甲胺水溶液的 1:1 混合物）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用铵开始脱保护（室温下 30 分钟），然后加入等量体积的 40% 甲胺水溶液，并像往常一样用 AMA 继续脱保护（65°C 下 10 分钟）。
Absorption and emission spectra of TET
TET phosphoramidite, 6-isomer相关产品
JOE phosphoramidite, 6-isomer
JOE dye phosphoramidite for synthesis of labeled oligonucleotides. JOE absorption and emission maxima are between maxima of FAM and TAMRA/ROX dyes.
JOE 染料亚磷酰胺用于合成标记的寡核苷酸。 JOE 吸收和发射最大值介于 FAM 和 TAMRA/ROX 染料的最大值之间。
HEX phosphoramidite, 6-isomer
HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.
用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。
General properties
| Appearance: | white solid foam |
| Molecular weight: | 981.72 |
| CAS number: | 877049-90-6 |
| Molecular formula: | C46H54N3Cl4O10P |
| IUPAC name: | 2′,4,7,7′-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl bis(2,2-dimethylpropanoate) |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 519 |
| ε, L⋅mol−1⋅cm−1: | 100000 |
| Emission maximum, nm: | 535 |
| Fluorescence quantum yield: | 0.47 |
| CF260: | 0.17 |
| CF280: | 0.09 |
Oligo synthesis details寡核苷酸合成细节
| Diluent:
冲淡： |
anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week).
无水乙腈（制备 0.1 М 溶液，储存 1 周）。 |
R6G phosphoramidite, 6-isomer
R6G phosphoramidite, 6-isomer,用于寡核苷酸合成的 R6G 亚磷酰胺，纯 6 异构体。
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- R6G phosphoramidite, 6-isomer
R6G phosphoramidite, 6-isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| C5260 | 100 mg | – | in stock |
| D5260 | 250 mg | 190.00$ | in stock |
| F5260 | 1 g | 590.00$ | in stock |
| G5260 | 5 g | 2390.00$ | in stock |
| H5260 | 10 g | please inquire | 10 days |
R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.
R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.
用于寡核苷酸合成的 R6G 亚磷酰胺，纯 6 异构体。
R6G（罗丹明 6G）是罗丹明家族的一种呫吨染料，具有高荧光量子产率和高摩尔消光系数。 相对于荧光素，6-R6G 的最大吸收 (518 nm) 和发射 (542 nm) 转移到长波区域。
R6G 可用作 DNA 测序和短串联重复 (STR) 分析中的 FRET 受体。 该染料通常用于生产用于定量 PCR 的 TaqMan 探针。 与 HEX 和 JOE 染料相比，R6G 与 BHQ1 猝灭剂组合在分子信标探针中具有更强的猝灭作用，显着降低了 PCR 中的背景荧光。
Usage:
Coupling: 10 minutes.
Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.
Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.
耦合：10 分钟。
脱保护：叔丁胺水溶液 (1:3 v/v) 在 55°С 下过夜。
由于 R6G 完全且不可逆地降解，因此不得使用氨水和 AMA（30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v）将修饰的寡核苷酸从固相载体上脱保护。
Absorption and emission spectra of R6G, 6-isomer
R6G phosphoramidite, 6-isomer相关产品
ROX azide, 5- isomer
ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.
ROX（又名罗丹明 X 或罗丹明 101）叠氮化物，用于通过 Click Chemistry 进行生物分子标记。
Cyanine3 azide
Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.
用于 Click Chemistry 的 Cyanine3 荧光染料的叠氮化物衍生物。
ROX NHS ester, 6- isomer
Pure isomer 6-ROX NHS ester for amine labeling.
用于胺标记的纯异构体 6-ROX NHS 酯。
General properties
| Appearance: | Yellowish foam |
| Molecular weight: | 949.93 |
| CAS number: | 1355330-47-0 |
| Molecular formula: | C46H54F6N5O8P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下最多保存 2 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 518 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.95 |
| CF260: | 0.18 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
Pyrene phosphoramidite dU
Pyrene phosphoramidite dU,Pyrene 是一种多环芳烃，以其嵌入 DNA 的能力而闻名。 芘具有强烈的蓝色荧光。 其参数很大程度上取决于荧光团微环境。 因此，芘的荧光光谱用于提取芘周围位点的结构信息。 两个非常接近的芘通常形成很容易被准分子荧光检测到的准分子。 芘也可以是其他荧光团（如苝）的 FRET 供体。
- Products
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- Pyrene phosphoramidite dU
Pyrene phosphoramidite dU
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 11460 | 100 mg | 150.00$ | in stock |
| 21460 | 250 mg | 320.00$ | in stock |
| 41460 | 1 g | 880.00$ | in stock |
| 61460 | 5 g | please inquire | in stock |
With this phosphoramidite, pyrene can be introduced into DNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows attaching pyrene fragment to 5′, internal, or 3′ position (using universal support).
This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.
Pyrene 是一种多环芳烃，以其嵌入 DNA 的能力而闻名。 芘具有强烈的蓝色荧光。 其参数很大程度上取决于荧光团微环境。 因此，芘的荧光光谱用于提取芘周围位点的结构信息。 两个非常接近的芘通常形成很容易被准分子荧光检测到的准分子。 芘也可以是其他荧光团（如苝）的 FRET 供体。
使用这种亚磷酰胺，可以通过自动合成将芘引入 DNA。 这种芘亚磷酰胺包含刚性连接到脱氧尿苷的烃部分。 该试剂允许将芘片段连接到 5’、内部或 3′ 位置（使用通用支架）。
这种亚酰胺不需要特殊的处理、偶联或脱保护条件。 推荐的稀释剂是乙腈。
Absorption and emission spectra of pyrene fluorophore
Pyrene phosphoramidite dU相关产品
Pyrenebutyric acid NHS ester
Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
芘 NHS 酯，用于用芘残基标记生物分子。 芘是一种形成准分子的荧光邻近探针，也是石墨固定的锚定基团。
Sulfo-Cyanine5 maleimide
Thiol reactive, water soluble cyanine dye for Cy5® channel.
用于 Cy5® 的硫醇活性水溶性花青染料 渠道。
Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
胺反应性 Cy5 活化酯，用于标记蛋白质、肽和其他分子。
General properties
| Appearance: | yellowish foam |
| Molecular weight: | 955.04 |
| CAS number: | 199920-17-7 |
| Molecular formula: | C57H55N4O8P |
| Solubility: | good in dichloromethane and acetonitrile
良好的二氯甲烷和乙腈 |
| Quality control: | NMR 1H (95%), 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 260; 282; 365; 392 |
| ε, L⋅mol−1⋅cm−1: | 12600; 21900; 16000; 14200 |
| Emission maximum, nm: | 460 |
| Fluorescence quantum yield: | ~0.1 |
Oligo synthesis details
| Diluent: | acetonitrile |
Perylene dU phosphoramidite
Perylene dU phosphoramidite,Oligonucleotide synthesis,Phosphoramidite，Perylene 是一种明亮且极耐光的荧光多环芳烃 (PAH) 标记，其量子产率接近定量。 由于荧光的低寿命，该探针不会形成准分子。,
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- DNA
- Phosphoramidites
- Perylene dU phosphoramidite
Perylene dU phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 14460 | 100 mg | 150.00$ | in stock |
| 24460 | 250 mg | 320.00$ | in stock |
| 44460 | 1 g | 880.00$ | in stock |
| 64460 | 5 g | please inquire | 10 days |
Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with a quantum yield approaching quantitative. Due to the low lifetime of fluorescence, this probe does not form excimers.
With this phosphoramidite, perylene can be introduced into DNA by automated oligonucleotide synthesis. Perylene is attached to the 5′ position of deoxyuridine (dU) through a triple bond and the fluorophore is electronically coupled to the deoxyuridine base. This coupling of dU and perylene makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.
This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent is acetonitrile.
Perylene 是一种明亮且极耐光的荧光多环芳烃 (PAH) 标记，其量子产率接近定量。 由于荧光的低寿命，该探针不会形成准分子。
使用这种亚磷酰胺，可以通过自动寡核苷酸合成将苝引入 DNA。 苝通过三键连接到脱氧尿苷 (dU) 的 5′ 位置，并且荧光团与脱氧尿苷碱基电子耦合。 dU 和苝的这种偶联使荧光对分子的 dU 部分的碱基配对敏感，从而可以区分完美和一个碱基错配的靶标。
这种亚酰胺不需要特殊的处理、偶联或脱保护条件。 推荐的稀释剂是乙腈。
Perylene dU phosphoramidite相关产品
HEX phosphoramidite, 6-isomer
HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.
用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。
Hexaethylene glycol phosphoramidite
Spacer phosphoramidite 18 (hexaethylene glycol amidite) provides a long hydrophilic linker for oligo synthesis.
间隔亚磷酰胺 18（六甘醇酰胺）为寡核苷酸合成提供了一个长的亲水接头。
Biotin phosphoramidite (hydroxyprolinol)
Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.
用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。
General properties
| Appearance: | orange solid |
| Molecular weight: | 1005.1 |
| CAS number: | 908117-78-2 |
| Molecular formula: | C61H57N4O8P |
| Solubility: | good in dichloromethane and acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 435; 408; 252 |
| ε, L⋅mol−1⋅cm−1: | 36000 |
| Emission maximum, nm: | 439; 467 |
| Fluorescence quantum yield: | 1.0 |
Oligo synthesis details
| Diluent: | acetonitrile |
JOE phosphoramidite, 6-isomer
JOE phosphoramidite, 6-isomer,用于寡核苷酸合成的 JOE 亚磷酰胺，纯 6-异构体 (6-JOE)。
- Products
- DNA
- Phosphoramidites
- JOE phosphoramidite, 6-isomer
JOE phosphoramidite, 6-isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 12160 | 100 mg | – | in stock |
| D2160 | 250 mg | 170.00$ | 10 days |
| F2160 | 1 g | 590.00$ | in stock |
| G2160 | 5 g | 2290.00$ | in stock |
| H2160 | 10 g | 3390.00$ | in stock |
| K2160 | 20 g | please inquire | 10 days |
JOE phosphoramidite for oligonucleotide synthesis, pure 6-isomer (6-JOE).
Fluorescent dye JOE is a fluorescein derivative containing two chlorine atoms and two methoxy groups. Its absorption and emission maxima are at 503 nm and 525 nm, respectively. By its spectral characteristics JOE is found in between FAM and TAMRA/ROX; therefore, this fluorophor is commonly used for multiplex detection, including that during DNA sequencing.
Our catalog also contains JOE phosphoramidite, 5-isomer. We compared qPCR probes containing different JOE isomers (5-JOE and 6-JOE) and did not find any significant differences between them.
用于寡核苷酸合成的 JOE 亚磷酰胺，纯 6-异构体 (6-JOE)。
荧光染料 JOE 是一种含有两个氯原子和两个甲氧基的荧光素衍生物。 其吸收和发射最大值分别为 503 nm 和 525 nm。 根据其光谱特性，JOE 介于 FAM 和 TAMRA/ROX 之间； 因此，这种荧光团通常用于多重检测，包括 DNA 测序期间的检测。
我们的目录还包含 JOE 亚磷酰胺，5 异构体。 我们比较了含有不同 JOE 异构体（5-JOE 和 6-JOE）的 qPCR 探针，并没有发现它们之间有任何显着差异。
Usage
Coupling: 6 minutes
Deprotection: standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).
耦合：6 分钟
脱保护：使用氢氧化铵的标准条件； 脱保护时间取决于寡核苷酸组成和核碱基保护基团（在 55°С 脱保护 17 小时会去除标准核碱基上的所有保护基团）。 AMA（30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v 的溶液）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用氢氧化铵开始脱保护（室温下 30 分钟），然后加入等体积的 40% 甲胺水溶液，并根据需要使用 AMA 继续脱保护（例如，在 65°C 下 10 分钟）。
Absorption and emission spectra of JOE
JOE phosphoramidite, 6-isomer相关产品
ROX NHS ester, 6- isomer
Pure isomer 6-ROX NHS ester for amine labeling.
用于胺标记的纯异构体 6-ROX NHS 酯。
Cyanine5.5 NHS ester
Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
Cy5.5 NHS 是远红/近红外胺活性染料。
BDP R6G azide
BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.
BDP R6G 是一种高性能硼二吡咯亚甲基荧光团，经过调整以匹配 R6G 染料的激发和发射通道。
General properties
| Appearance: | white solid |
| Molecular weight: | 972.88 |
| Molecular formula: | C48H60N3Cl2O12P |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Oligo synthesis details
| Diluent: | acetonitrile |
JOE phosphoramidite, 5-isomer
Oligonucleotide synthesis, Phosphoramidite, JOE,JOE 是一种呫吨染料，具有两个氯和两个甲氧基取代基的荧光素衍生物。 这种荧光团是寡核苷酸的有用标记。 其吸收和发射光谱位于 FAM 和 TAMRA 之间。
- Products
- DNA
- Phosphoramidites
- JOE phosphoramidite, 5-isomer
JOE phosphoramidite, 5-isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| C1160 | 100 mg | – | 5 days |
| D1160 | 250 mg | 170.00$ | 5 days |
| F1160 | 1 g | 590.00$ | in stock |
| G1160 | 5 g | 2290.00$ | in stock |
| H1160 | 10 g | 3390.00$ | in stock |
JOE is a xanthene dye, fluorescein derivative possessing two chloro and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.
The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains pure isomer of 5-JOE dye.
JOE 是一种呫吨染料，具有两个氯和两个甲氧基取代基的荧光素衍生物。 这种荧光团是寡核苷酸的有用标记。 其吸收和发射光谱位于 FAM 和 TAMRA 之间。
可以使用这种亚磷酰胺将荧光团引入寡核苷酸中。 它可以承受标准的铵解封条件。 本产品含有5-JOE染料的纯异构体。
Absorption and emission spectra of JOE dye
Oligonucleotide synthesis, Phosphoramidite, JOE相关产品
dsGreen for Real-Time PCR, 100×
Stock solution of dsGreen for Real-Time PCR, 100×
用于实时 PCR 的 dsGreen 储备溶液，100×
Sulfo-Cyanine5.5 azide
Sulfo-Cyanine5.5 azide is a derivative of water soluble far red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper free Click chemistry. The reagent possesses high aqueous solubility and hydrophilicity, and recommended for the labeling of biomolecules in aqueous environment.
Sulfo-Cy5.5 azide 是水溶性远红荧光花青染料的衍生物。 叠氮基可以通过铜催化或无铜点击化学进行缀合。 该试剂具有高水溶性和亲水性，推荐用于水环境中生物分子的标记。
Sulfo-Cyanine5.5 amine
Sulfo-Cyanine5.5 is a far red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. The dye displays good brightness in far red region due to outstanding molar extinction coefficient. This is amine derivative which is reactive towards electrophiles. It can also be used for enzymatic transamination labeling.
Sulfo-Cy5.5 是一种远红/近红外荧光团，具有高亲水性和水溶性。 由于出色的摩尔消光系数，该染料在远红色区域显示出良好的亮度。 这是对亲电试剂有反应性的胺衍生物。 它也可用于酶促转氨标记。
General properties
| Appearance: | colorless solid |
| Molecular weight: | 972.88 |
| Molecular formula: | C48H60N3Cl2O12P |
| Solubility: | good in DCM, acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 554 |
| Fluorescence quantum yield: | 0.61 |
| CF260: | 0.36 |
| CF280: | 0.28 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
Inosine (dI) phosphoramidite
Inosine (dI) phosphoramidite,脱氧肌苷是在天然核酸中遇到的次要碱基。 它与 A、C 和 T/U 碱基形成碱基对。 使用这种亚磷酰胺可以合成含有 2′-脱氧肌苷的修饰 DNA 寡核苷酸。
- Products
- DNA
- Phosphoramidites
- Inosine (dI) phosphoramidite
Inosine (dI) phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 319-1g | 1 g | 190.00$ | in stock |
| 319-5g | 5 g | 750.00$ | in stock |
Deoxyinosine is a minor base that is encountered in natural nucleic acids. It forms base pairs with A, C and T/U bases. The modified DNA oligonucleotides containing 2′-deoxyinosine can be synthesized using this phosphoramidite.
脱氧肌苷是在天然核酸中遇到的次要碱基。 它与 A、C 和 T/U 碱基形成碱基对。 使用这种亚磷酰胺可以合成含有 2′-脱氧肌苷的修饰 DNA 寡核苷酸。
Inosine (dI) phosphoramidite相关产品
Hexaethylene glycol phosphoramidite
Spacer phosphoramidite 18 (hexaethylene glycol amidite) provides a long hydrophilic linker for oligo synthesis.
间隔亚磷酰胺 18（六甘醇酰胺）为寡核苷酸合成提供了一个长的亲水接头。
Biotin phosphoramidite (hydroxyprolinol)
Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.
用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。
R6G phosphoramidite, 6-isomer
Phosphoramidite for synthesis of oligonucleotides labeled at the 5′-terminus with rhodamine 6G (R6G). Pure 6-isomer.
亚磷酰胺用于合成在 5′ 末端用罗丹明 6G (R6G) 标记的寡核苷酸。 纯 6 异构体。
General properties
| Appearance: | white solid |
| Molecular weight: | 754.81 |
| CAS number: | 141684-35-7 |
| Molecular formula: | C40H47N6O7P |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
Hexaethylene glycol phosphoramidite
Hexaethylene glycol phosphoramidite,Spacer phosphoramidite 18 (hexaethylene glycol phosphoramidite) is an amidite reagent for oligonucleotide synthesis.
- Products
- DNA
- Phosphoramidites
- Hexaethylene glycol phosphoramidite
Hexaethylene glycol phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 26160 | 250 mg | 70.00$ | in stock |
| 46160 | 1 g | 210.00$ | in stock |
Spacer phosphoramidite 18 (hexaethylene glycol phosphoramidite) is an amidite reagent for oligonucleotide synthesis. It provides a long hydrophilic linker that can be used for the synthesis of Scorpion-type qPCR probes, for spacing biotin from oligo strand, and for other applications requiring spatial separation of modifier and oligo.
间隔亚磷酰胺 18（六甘醇亚磷酰胺）是一种用于寡核苷酸合成的亚酰胺试剂。 它提供了一个长的亲水性接头，可用于合成 Scorpion 型 qPCR 探针，用于将生物素与寡核苷酸链分开，以及用于需要修饰剂和寡核苷酸空间分离的其他应用。
Hexaethylene glycol phosphoramidite相关产品
DMS(O)MT aminolink C6
An amidite for the introduction of amino group.
用于引入氨基的亚酰胺。
Alkyne Phosphoramidite, 5′-terminal
Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.
用于合成寡核苷酸的炔亚磷酰胺，可用于点击化学。
Inosine (dI) phosphoramidite
Dimethoxytrityl deoxyinosine (Dmt-dI) phosphoramidite for the synthesis of modified DNA oligonucleotides.
二甲氧基三苯甲基脱氧肌苷 (Dmt-dI) 亚磷酰胺，用于合成修饰的 DNA 寡核苷酸。
General properties
| Appearance: | colorless/yellowish oil |
| Molecular weight: | 784.92 |
| CAS number: | 125607-09-2 |
| Molecular formula: | C42H61N2O10P |
| Solubility: | good in most organic solvents |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), testing in oligo sythesis |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
HEX phosphoramidite, 6-isomer
HEX phosphoramidite, 6-isomer,HEX（六氯荧光素）是一种荧光素衍生物，在黄色光谱范围内发射（最大吸收在 533 nm，最大发射在 549 nm）。
- Products
- DNA
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- HEX phosphoramidite, 6-isomer
HEX phosphoramidite, 6-isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| CB260 | 100 mg | – | 10 days |
| DB260 | 250 mg | 360.00$ | in stock |
| FB260 | 1 g | 760.00$ | in stock |
| GB260 | 5 g | 3490.00$ | 21 days |
| HB260 | 10 g | please inquire | 21 days |
HEX phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
HEX (hexachlorofluorescein) is a fluorescein derivative with emission in the yellow spectrum range (absorption maximum at 533 nm, emission maximum at 549 nm).
HEX phosphoramidite is used for synthesis of fluorescent-labeled primers and hybridization probes such as TaqMan, Molecular Beacon, and Scorpion for qPCR. HEX is most effectively quenched by non-fluorescent DusQ1 dark quencher because of significant overlapping of their spectra (convenient for use with DusQ1 CPG 500 solid support with a pore size of 500 Å). Many automated sequencers based on capillary gel electrophoresis have a detection channel for HEX. Therefore, this phosphoramidite is commonly used for synthesis of 5’-labeled oligonucleotides for fragment analysis, particularly for microsatellite analysis, when microsatellite loci are amplified using a fluorescent-labeled forward primer and a non-labeled reverse primer.
用于寡核苷酸合成的 HEX 亚磷酰胺，纯 6 异构体。
HEX（六氯荧光素）是一种荧光素衍生物，在黄色光谱范围内发射（最大吸收在 533 nm，最大发射在 549 nm）。
HEX 亚磷酰胺用于合成荧光标记的引物和杂交探针，例如用于 qPCR 的 TaqMan、Molecular Beacon 和 Scorpion。 HEX 最有效地被非荧光 DusQ1 暗猝灭剂猝灭，因为它们的光谱有显着重叠（便于与孔径为 500 Å 的 DusQ1 CPG 500 固体支持物一起使用）。 许多基于毛细管凝胶电泳的自动测序仪都有一个 HEX 检测通道。 因此，当使用荧光标记的正向引物和未标记的反向引物扩增微卫星基因座时，这种亚磷酰胺通常用于合成 5′ 标记的寡核苷酸以进行片段分析，特别是用于微卫星分析。
Usage
Coupling: 3 min.
Deprotection: standard conditions using 25% ammonium; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of concentrated aqueous ammonium/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).
耦合：3 分钟。
脱保护：使用 25% 铵的标准条件； 脱保护时间取决于寡核苷酸组成和核碱基保护基团（在 55°C 下脱保护 17 小时会去除标准核碱基上的所有保护基团）。 AMA（浓铵水溶液/40% 甲胺水溶液 1:1 v/v）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用氢氧化铵开始脱保护（室温下 30 分钟），然后加入等体积的 40% 甲胺水溶液，并根据需要使用 AMA 继续脱保护（例如，在 65°C 下 10 分钟）。
Absorption and emission spectra of HEX
HEX phosphoramidite, 6-isomer相关产品
Cyanine5 phosphoramidite
Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.
Cy5 的亚磷酰胺衍生物，用于寡核苷酸合成。
BDP TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.
具有叠氮基的硼二吡咯亚甲基染料。 光谱特性类似于 TAMRA 染料。 由于荧光寿命长，BDP TMR 特别适用于荧光偏振测量。
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
Catalyst for bioconjugate synthesis by Click Chemistry.
Click Chemistry 用于生物共轭合成的催化剂。
General properties
| Appearance: | off white solid |
| Molecular weight: | 1050.61 |
| CAS number: | 1360547-55-2 |
| Molecular formula: | C46H52N3Cl6O10P |
| Solubility: | Good solubility in acetonitrile and DCM. |
| Quality control: | NMR 1H and 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 533 |
| ε, L⋅mol−1⋅cm−1: | 87770 |
| Emission maximum, nm: | 549 |
| Fluorescence quantum yield: | 0.57 |
| CF260: | 0.30 |
| CF280: | 0.13 |
Oligo synthesis details
| Diluent: | anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week). |
FAM-dT phosphoramidite
FAM-dT phosphoramidite,这种亚磷酰胺允许荧光素 (FAM) 在合成过程中通过亚磷酰胺方法引入寡核苷酸序列的任何位置（在链的中间，在 5′ 和 3′ 末端）。
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FAM-dT phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 13460 | 100 mg | – | in stock |
| 23460 | 250 mg | 390.00$ | in stock |
| 43460 | 1 g | 1340.00$ | in stock |
| 63460 | 5 g | 5400.00$ | in stock |
| 83460 | 10 g | please inquire | in stock |
This phosphoramidite allows fluorescein (FAM) to be introduced into any position in the oligonucleotide sequence during synthesis by the phosphoramidite method (in the middle of the chain, at the 5’- and 3’-terminus). The reagent is a conjugate of deoxythymidine phosphoramidite and 6-isomer of FAM. Modification is performed during oligonucleotide synthesis by substituting standard dT phosphoramidite with fluorescein-dT phosphoramidite. This modification does not affect exonuclease or polymerase activity.
For modification with fluorescein at the 5’-terminus use FAM phosphoramidite, 6-isomer.
这种亚磷酰胺允许荧光素 (FAM) 在合成过程中通过亚磷酰胺方法引入寡核苷酸序列的任何位置（在链的中间，在 5′ 和 3′ 末端）。 该试剂是脱氧胸苷亚磷酰胺和 FAM 6-异构体的结合物。 在寡核苷酸合成过程中通过用荧光素-dT 亚磷酰胺代替标准 dT 亚磷酰胺进行修饰。 这种修饰不影响核酸外切酶或聚合酶活性。
要在 5′-末端使用荧光素进行修饰，请使用 FAM 亚磷酰胺，6-异构体。
Usage
Coupling: 10 minutes
Deprotection: standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).
耦合：10 分钟
脱保护：使用氢氧化铵的标准条件； 脱保护时间取决于寡核苷酸组成和核碱基保护基团（在 55°С 脱保护 17 小时会去除标准核碱基上的所有保护基团）。 AMA（30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v 的溶液）可用于形成约 5% 的非荧光副产物。 为避免形成副产物，用氢氧化铵开始脱保护（室温下 30 分钟），然后加入等体积的 40% 甲胺水溶液，并根据需要使用 AMA 继续脱保护（例如，在 65°C 下 10 分钟）。
Absorption and emission spectra of FAM
FAM-dT phosphoramidite相关产品
Cyanine5 azide
Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.
Cy5 azide 是一种用 Cyanine5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。
Alkyne dT phosphoramidite
Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.
亚磷酰胺修饰剂，用于合成在链中间含有炔烃修饰的寡核苷酸。
DusQ1 amidite, 5′-terminal
General properties
| Appearance: | off white powder |
| Molecular weight: | 1423.54 |
| Molecular formula: | C79H87N6O17P |
| Solubility: | good in acetonitrile, DCM |
| Quality control: | NMR 1H, NMR 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
FAM Phosphoramidite, 6-Isomer
FAM Phosphoramidite, 6-Isomer,Sandard fluorescein (FAM) 亚磷酰胺，用于 5′-末端寡核苷酸标记，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。
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- FAM Phosphoramidite, 6-Isomer
FAM Phosphoramidite, 6-Isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| D5160 | 250 mg | – | in stock |
| F5160 | 1 g | 190.00$ | in stock |
| G5160 | 5 g | 740.00$ | in stock |
| H5160 | 10 g | 1290.00$ | in stock |
| K5160 | 50 g | please inquire | 21 days |
Sandard fluorescein (FAM) phosphoramidite for 5′-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.
Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.
Sandard fluorescein (FAM) 亚磷酰胺，用于 5′-末端寡核苷酸标记，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。
寡核苷酸制造商可提供样品和特别优惠。 联系我们获取该试剂的批量供应。
FAM absorbance and emission spectra
FAM Phosphoramidite, 6-Isomer相关产品
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
Catalyst for bioconjugate synthesis by Click Chemistry.
Click Chemistry 用于生物共轭合成的催化剂。
Phosphate CPG 1000 solid support
Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.
磷酸盐修饰剂 CPG，用于固相合成含有 3 个磷酸基修饰的长（最多 120 聚体）寡核苷酸。
BDP FL azide
BDP FL is a bright and photostable borondipyrromethene dye for fluorescein channel. This is a dye azide for Click Chemistry.
BDP FL 是一种用于荧光素通道的明亮且光稳定的硼二吡咯亚甲基染料。 这是一种用于 Click Chemistry 的染料叠氮化物。
General properties
| Appearance: | white foam |
| Molecular weight: | 843.94 |
| CAS number: | 204697-37-0 |
| Molecular formula: | C46H58N3O10P |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate. 储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下最多保存 2 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | coupling time 10 min |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine |
相关文献：
- Zhou, Z.; Liu, S.; Zhang, Y.; Yang, X.; Ma, Y.; Guan, Z.; Wu, Y.; Zhang, L.; Yang, Z. Reductive nanocomplex encapsulation of cRGD-siRNA conjugates for enhanced targeting to cancer cells. International Journal of Nanomedicine, 2017, 12, 7255–7272. doi: 10.2147/ijn.S136726
FAM Phosphoramidite, 5-Isomer
FAM Phosphoramidite, 5-Isomer,用于 5′-末端寡核苷酸标记的标准荧光素 (FAM) 亚磷酰胺，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。
- Products
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- Phosphoramidites
- FAM Phosphoramidite, 5-Isomer
FAM Phosphoramidite, 5-Isomer
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 2639-500mg | 500 mg | – | 10 days |
| 2639-1g | 1 g | 190.00$ | in stock |
| 2639-5g | 5 g | 740.00$ | in stock |
| 2639-10g | 10 g | 1290.00$ | in stock |
| 2639-50g | 50 g | please inquire | 21 days |
Standard fluorescein (FAM) phosphoramidite for 5′-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.
Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.
用于 5′-末端寡核苷酸标记的标准荧光素 (FAM) 亚磷酰胺，高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。
寡核苷酸制造商可提供样品和特别优惠。 联系我们获取该试剂的批量供应。
FAM Phosphoramidite, 5-Isomer相关产品
Phosphate CPG 500 solid support
CPG solid support for the synthesis of 3′-phosphorylated oligonucleotides.
用于合成 3′-磷酸化寡核苷酸的 CPG 固体支持物。
HEX phosphoramidite, 6-isomer
HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.
用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。
Add this product to your cart andGeneral properties
| Appearance: | off white solid |
| Molecular weight: | 843.94 |
| CAS number: | 147566-42-5 |
| Molecular formula: | C46H58N3O10P |
| Solubility: | Good solubility in acetonitrile and DCM |
| Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | coupling time 10 min |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine |
相关文献：
- Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212
DusQ2 phosphoramidite
DusQ2 phosphoramidite,DusQ2 是一种黑色猝灭剂染料，用于合成带有 5′-猝灭剂的 qPCR 的双标记寡核苷酸探针。 这种猝灭剂非常适合 HEX、JOE、ROX、Cyanine5 和其他在光谱的橙色和红色部分发射的染料。
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- DusQ2 phosphoramidite
DusQ2 phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 1473-100mg | 100 mg | 290.00$ | in stock |
| 1473-500mg | 500 mg | 990.00$ | in stock |
| 1473-1g | 1 g | 1690.00$ | in stock |
| 1473-10g | 10 g | please inquire | 21 days |
DusQ2 is a black quencher dye for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5′-quencher. This quencher is ideal for HEX, JOE, ROX, Cyanine5 and other dyes with the emission in orange and red part of the spectrum.
The primary phosphoramidite group couples with hydroxyls efficiently in oligonucleotide synthesis.
DusQ2 是一种黑色猝灭剂染料，用于合成带有 5′-猝灭剂的 qPCR 的双标记寡核苷酸探针。 这种猝灭剂非常适合 HEX、JOE、ROX、Cyanine5 和其他在光谱的橙色和红色部分发射的染料。
初级亚磷酰胺基团在寡核苷酸合成中有效地与羟基偶联。
Absorption spectrum of DusQ2
DusQ2 phosphoramidite相关产品
R6G azide, 5-isomer
Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of Click chemistry.
罗丹明 6G 是一种已用于 DNA 测序和 qPCR 的呫吨染料。 这种叠氮化物衍生物用于通过点击化学将染料连接到 DNA 和其他分子上。
DusQ1 phosphoramidite
Cyanine5 phosphoramidite
Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.
Cy5 的亚磷酰胺衍生物，用于寡核苷酸合成。
General properties
| Appearance: | dark colored solid |
| Molecular weight: | 997.08 |
| CAS number: | 374591-98-7 |
| Molecular formula: | C54H61N8O9P |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| ε, L⋅mol−1⋅cm−1: | 34000 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Diluent: | 20% THF / acetonitrile |
| Coupling conditions: | 6 min coupling time; 3 min oxidation time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
DusQ2 dT phosphoramidite
DusQ2 dT phosphoramidite,
- Products
- DNA
- Phosphoramidites
- DusQ2 dT phosphoramidite
DusQ2 dT phosphoramidite
| 货号 | 规格 | 价格 | 货号 |
|---|---|---|---|
| 1451-100mg | 100 mg | 340.00$ | in stock |
| 1451-500mg | 500 mg | 1390.00$ | in stock |
| 1451-1g | 1 g | 2290.00$ | in stock |
DusQ2 dT phosphoramidite相关产品
Cyanine5 azide
Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.
Cy5 azide 是一种用 Cy5（一种流行的荧光染料）进行 Click Chemistry 标记的试剂。
FAM Phosphoramidite, 5-Isomer
Single isomer 5-FAM phosphoramidite for the synthesis of modified oligonucleotides.
用于合成修饰寡核苷酸的单一异构体 5-FAM 亚磷酰胺。
FAM-dT phosphoramidite
Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.
亚磷酰胺用于合成在序列中间用荧光素 (FAM) 标记的寡核苷酸。
General properties
| Appearance: | черный порошок |
| Molecular weight: | 1,413.55 |
| Quality control: | NMR 1H, 31P,HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Coupling conditions: | 6 min coupling time; 3 min oxidation time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
DusQ1 phosphoramidite
DusQ1 phosphoramidite,Oligonucleotide synthesis, Phosphoramidite
- Products
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- Phosphoramidites
- DusQ1 phosphoramidite
DusQ1 phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 1470-100mg | 100 mg | 290.00$ | in stock |
| 1470-500mg | 500 mg | 990.00$ | in stock |
| 1470-1g | 1 g | 1690.00$ | in stock |
DusQ1 phosphoramidite相关产品
Alkyne dT phosphoramidite
Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.
亚磷酰胺修饰剂，用于合成在链中间含有炔烃修饰的寡核苷酸。
Cyanine5.5 azide
Far red / near infrared fluorescent Cyanine5.5 dye derivative for Click Chemistry.
用于 Click Chemistry 的远红/近红外荧光 Cy5.5 染料衍生物。
Add this product to your cart andDusQ2 dT phosphoramidite
General properties
| Appearance: | black foam |
| Molecular weight: | 995.11 |
| CAS number: | 1430816-77-5 |
| Quality control: | NMR 1H, 31P,HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | 20% THF / acetonitrile |
| Coupling conditions: | 6 min coupling time; 3 min oxidation time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
DusQ1 dT phosphoramidite
DusQ1 dT phosphoramidite,耦合条件,6分钟耦合时间； 3分钟氧化时间,裂解条件,氨水，室温下 2 小时.
- Products
- DNA
- Phosphoramidites
- DusQ1 dT phosphoramidite
DusQ1 dT phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 1452-100mg | 100 mg | 340.00$ | in stock |
| 1452-500mg | 500 mg | 1390.00$ | 10 days |
| 1452-1g | 1 g | 2290.00$ | in stock |
DusQ1 dT phosphoramidite相关产品
Alkyne CPG modifier 500
Solid support for the synthesis of 3′-modified oligonucleotides with alkyne group for Click Chemistry.
为 Click Chemistry 合成具有炔基的 3′-修饰寡核苷酸提供坚实支持。
Alkyne Phosphoramidite, 5′-terminal
Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.
用于合成寡核苷酸的炔亚磷酰胺，可用于点击化学。
Add this product to your cart andDusQ1 CPG 500
Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
可控孔玻璃 (CPG) 500 固体支持物，用于合成具有 3′-末端 DusQ1 淬灭剂的寡核苷酸。 与标准解块条件兼容。
General properties
| Appearance: | черный порошок 黑火药 |
| Molecular weight: | 1,411.58 |
| Quality control: | NMR 1H, 31P,HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Coupling conditions:
耦合条件 |
6 min coupling time; 3 min oxidation time
6分钟耦合时间； 3分钟氧化时间 |
| Cleavage conditions:
裂解条件 |
ammonia, 2 h at room temperature
氨水，室温下 2 小时 |
| Deprotection conditions: | identical to protected nucleobases
与受保护的核碱基相同 |
DusQ1 amidite, 5′-terminal
DusQ1 amidite, 5′-terminal,储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。
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- DusQ1 amidite, 5′-terminal
DusQ1 amidite, 5′-terminal
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 1D260 | 100 mg | 290.00$ | in stock |
| 2D260 | 250 mg | 590.00$ | in stock |
| 3D260 | 500 mg | 990.00$ | in stock |
| 4D260 | 1 g | 1690.00$ | in stock |
| 6D260 | 5 g | 6790.00$ | in stock |
| 7D260 | 10 g | please inquire | 10 days |
Absorption spectrum of DusQ1
DusQ1 amidite, 5′-terminal相关产品
DusQ1 phosphoramidite
FAM-dT phosphoramidite
Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.
Add this product to your cart anddsGreen Gel Staining Solution, 10000×
dsGreen for staining dsDNA in gels.
General properties
| Appearance: | dark colored solid |
| Molecular weight: | 676.75 |
| Molecular formula: | C34H45N8O5P |
| Solubility: | good in acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), coupling test |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 35935 |
| CF260: | 0.17 |
| CF280: | 0.10 |
Oligo synthesis details
| Diluent: | acetonitrile |
DMS(O)MT aminolink C6
DMS(O)MT aminolink C6,Aminolink 亚磷酰胺用于寡核苷酸合成。 DMS(O)MT 是一种类似于传统 MMT 的特殊保护基团，但设计为它的改进替代品。 DMS(O)MT 氨基连接与标准偶联、解封闭和纯化方案完全兼容。
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DMS(O)MT aminolink C6
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 23260 | 250 mg | 90.00$ | in stock |
| 43260 | 1 g | 260.00$ | in stock |
| 63260 | 5 g | 950.00$ | in stock |
| 83260 | 10 g | 1890.00$ | 10 days |
| 93260 | 25 g | please inquire | 10 days |
In case of cartridge purification, DMS(O)MT protection allows for 2-3 fold increase of oligonucleotide yield, making this reagent a unique solution for high-throughput manufacturing of amino-oligonucleotides.
Aminolink 亚磷酰胺用于寡核苷酸合成。 DMS(O)MT 是一种类似于传统 MMT 的特殊保护基团，但设计为它的改进替代品。 DMS(O)MT 氨基连接与标准偶联、解封闭和纯化方案完全兼容。 然而，由于更高的稳定性和更好的寡核苷酸回收率，DMS(O)MT 组更适合于 DMT-on 和氨基寡核苷酸的柱纯化。 含有 DMS(O)MT 的寡核苷酸在溶液中比 MMT 类似物更稳定。
在柱纯化的情况下，DMS(O)MT 保护可使寡核苷酸产量增加 2-3 倍，使该试剂成为高通量生产氨基寡核苷酸的独特解决方案。
DMS(O)MT aminolink C6相关产品
FAM Phosphoramidite, 6-Isomer
Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.
用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。
Add this product to your cart andR6G phosphoramidite, 6-isomer
Phosphoramidite for synthesis of oligonucleotides labeled at the 5′-terminus with rhodamine 6G (R6G). Pure 6-isomer.
亚磷酰胺用于合成在 5′ 末端用罗丹明 6G (R6G) 标记的寡核苷酸。 纯 6 异构体。
Add this product to your cart andPhosphate CPG 500 solid support
CPG solid support for the synthesis of 3′-phosphorylated oligonucleotides.
用于合成 3′-磷酸化寡核苷酸的 CPG 固体支持物。
Add this product to your cart andGeneral properties
| Appearance: | yellowish viscous oil |
| Molecular weight: | 681.86 |
| CAS number: | 1173109-53-9 |
| Molecular formula: | C37H52N3O5PS |
| Solubility: | soluble in acetonitrile, dichloromethane |
| Quality control: | NMR 1H, 31P, HPLC (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonia, 2 h at room temperature; if an oligonucleotide with trityl group needs to be evaporated, add small amount of Tris base before it, or trityl group can be lost. |
| Deprotection conditions: | identical to protected nucleobases. Trityl group can be removed by acidic cleavage in either 80% acetic acid, or on C18 cartridge during purification using 2% to 4% trifluoroacetic acid. |
相关文献：
- Aparin, I.O.; Farzan, V.M.; Veselova, O.A.; Chistov, A.A.; Podkolzin, A.T.; Ustinov, A.V.; Shipulin, G.A.; Formanovsky, A.A.; Korshun, V.A.; Zatsepin, T.S. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst, 2016, 141, 1331–1338. doi: 10.1039/c5an01767j
Cyanine5.5 phosphoramidite, 5′-terminal
Cyanine5.5 phosphoramidite, 5′-terminal,Cy5.5 是一种荧光团，在光谱的远红色范围内发射。 这种染料可用于多重 qPCR。 商用六通道 qPCR 仪器通常有一个用于 Cy5.5 的通道。
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- Cyanine5.5 phosphoramidite, 5′-terminal
Cyanine5.5 phosphoramidite, 5′-terminal
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 2680-100mg | 100 mg | – | 7 days |
| 2680-250mg | 250 mg | 290.00$ | 10 days |
| 2680-1g | 1 g | 950.00$ | in stock |
| 2680-10g | 10 g | 6750.00$ | 21 days |
This phosphoramidite can be used for the synthesis of 5′-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.
The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.
Cy5.5 是一种荧光团，在光谱的远红色范围内发射。 这种染料可用于多重 qPCR。 商用六通道 qPCR 仪器通常有一个用于 Cy5.5 的通道。
该亚磷酰胺可用于通过在寡核苷酸合成仪中直接标记来合成5′-标记的寡核苷酸。
与仲碳原子相连的亚磷酰胺官能团结构提供了额外的稳定性，可防止 Arbuzov 重排。 与衍生自伯醇的亚磷酰胺相比，这有助于在寡核苷酸合成仪中在较长的存储时间内保持偶联性能。
Absorption and emission spectra of Cyanine5.5
Cyanine5.5 phosphoramidite, 5′-terminal相关产品
Phosphate CPG 1000 solid support
Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.
磷酸盐修饰剂 CPG，用于固相合成含有 3 个磷酸基修饰的长（最多 120 聚体）寡核苷酸。
Cyanine5 phosphoramidite
Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.
Cy5 的亚磷酰胺衍生物，用于寡核苷酸合成。
FAM CPG 500, 6-isomer
Controlled pore glass for the synthesis of modified oligonucleotides containing 3′-fluorescein (FAM) modification.
用于合成含有 3′-荧光素 (FAM) 修饰的修饰寡核苷酸的可控孔玻璃。
General properties
| Appearance: | dark colored solid |
| Molecular weight: | 916.61 |
| Molecular formula: | C55H71N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (85%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 694 |
| ε, L⋅mol−1⋅cm−1: | 198000 |
| Emission maximum, nm: | 710 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.07 |
| CF280: | 0.03 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible |
Cy5 phosphoramidite
Cy5 phosphoramidite,Cy5 是 qPCR 中使用的染料之一。 在许多 qPCR 机器上都可以找到相应的过滤器集。
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Cyanine5 phosphoramidite
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 1740-100mg | 100 mg | – | in stock |
| 1740-250mg | 250 mg | 290.00$ | 10 days |
| 1740-1g | 1 g | 950.00$ | in stock |
| 1740-10g | 10 g | 6750.00$ | 10 days |
Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.
This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5′-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.
Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement – a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases stability of the reagent, maintaining efficient coupling over longer time.
Cy5 是 qPCR 中使用的染料之一。 在许多 qPCR 机器上都可以找到相应的过滤器集。
这种反应性衍生物可用于合成在 5′ 端具有花青 5 的 qPCR 探针，这是荧光团最典型的位置。 这是一种终止性非核苷试剂。
该试剂中的酰胺基与二级氧结合。 这为 Arbuzov 重排提供了额外的稳定性 – 这种反应会导致寡核苷酸合成器中溶液中的亚酰胺劣化。 这种分子设计增加了试剂的稳定性，在更长的时间内保持有效的耦合。
Absorption and emission spectra of Cyanine5
Cy5 phosphoramidite相关产品
FAM Phosphoramidite, 6-Isomer
Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.
用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。
Cyanine3 phosphoramidite 5′
Phosphoramidite for the synthesis of 5’-cyanine3-labeled oligonucleotides.
用于合成 5′-菁 3 标记寡核苷酸的亚磷酰胺。
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
Catalyst for bioconjugate synthesis by Click Chemistry.
Click Chemistry 用于生物共轭合成的催化剂。
General properties
| Appearance: | dark colored solid |
| Molecular weight: | 816.49 |
| Molecular formula: | C47H67N5ClO3P |
| Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended
推荐 6 分钟耦合时间 |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
建议在 +4°C 或超温和保护基团下放置 48 小时； 24 小时在 rt 可能 |
Cyanine3 phosphoramidite 5′
Cyanine3 phosphoramidite 5′,Cy3 是一种荧光团，广泛用于分子生物学实验，例如寡核苷酸标记和寡核苷酸检测。根据其光谱特性，Cyanine3 是 Cy™3 的类似物，在黄色光谱范围内的荧光最大值为 570 nm。
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Cyanine3 phosphoramidite 5′
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 2771-100mg | 100 mg | – | in stock |
| 2771-250mg | 250 mg | 290.00$ | 10 days |
| 2771-1g | 1 g | 950.00$ | in stock |
| 2771-10g | 10 g | 6750.00$ | 21 days |
Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is an analog of Cy™3 with a fluorescence maximum at 570 nm in the yellow spectrum range.
Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.
This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.
Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.
Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.
Cy3 是一种荧光团，广泛用于分子生物学实验，例如寡核苷酸标记和寡核苷酸检测。根据其光谱特性，Cyanine3 是 Cy™3 的类似物，在黄色光谱范围内的荧光最大值为 570 nm。
花青 3 亚磷酰胺 5′ 用于寡核苷酸合成，以生产 5′-花青 3 标记的寡核苷酸。该试剂与各种寡核苷酸合成仪兼容。
这种亚磷酰胺可用于合成荧光标记的引物和杂交探针，例如 TaqMan 和 Molecular Beacon。可以在 TAMRA 通道中的多重实时 PCR 中检测到此类标记的探针。
冷凝：3 分钟。在氧化步骤中使用 0.02 碘溶液以避免花青染料降解。
脱保护：在室温下用 30% 的铵水溶液。建议使用带有不稳定保护基团的核酸碱基在低于 55°C 的条件下脱保护不超过 2 小时。 AMA（30% 铵水溶液/40% 甲胺水溶液 1:1 (v/v)）可在乙酰脱氧胞苷存在下在 65°C 下使用 10 分钟。如果在合成过程中使用带有二甲基甲脒保护基团的脱氧胍，则在 65°C 下用 30% 铵水溶液脱保护 2 小时。如果在合成过程中使用带有异丁酰基保护基团的脱氧胍，则在室温下脱保护 24-36 小时。
Cyanine3 phosphoramidite 5’相关产品
Cyanine5.5 phosphoramidite, 5′-terminal
Amidite for the synthesis of oligonucleotides and qPCR probes bearing 5′-terminal dye Cyanine5.5. Useful dye for multiplex qPCR.
Amidite 用于合成带有 5′-末端染料 Cyanine5.5 的寡核苷酸和 qPCR 探针。 用于多重 qPCR 的有用染料。
FAM Phosphoramidite, 6-Isomer
Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.
用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。
Cyanine5 phosphoramidite
Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.
Cyanine5 的亚磷酰胺衍生物，用于寡核苷酸合成。
General properties
| Appearance: | red powder |
| Molecular weight: | 841.81 |
| Molecular formula: | C45H65N5BF4O3P |
| Solubility: | good in acetonitrile, dichloromethane |
| Quality control: | NMR 1H, 31P, HPLC-MS (80%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 555 |
| ε, L⋅mol−1⋅cm−1: | 150000 |
| Emission maximum, nm: | 570 |
| Fluorescence quantum yield: | 0.31 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | 6 min coupling time recommended |
| Deprotection conditions: | recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible
建议在 +4°C 或超温和保护基团下放置 48 小时； 24 小时在 rt 可能 |
Chemical phosphorylation reagent for oligonucleotide synthesis
Chemical phosphorylation reagent for oligonucleotide synthesis,用于合成 5′-磷酸化寡核苷酸的化学磷酸化试剂。 该试剂的结构中含有一个 DMT 基团，可以在 C18 小柱上或通过反相色谱法纯化寡核苷酸。 要产生 5′-磷酸化寡核苷酸，请去除 DMT 基团，然后用稀释的铵溶液 (0.1 M) 去保护磷酸基团。 即使在温和的碱性条件下，磷酸基团的脱保护也能快速有效地进行，因此该试剂可用于 DMT-Off 合成，例如 RNA 合成，或用于需要温和脱保护条件的染料亚磷酰胺。
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- Chemical phosphorylation reagent for oligonucleotide synthesis
Chemical phosphorylation reagent for oligonucleotide synthesis
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 21560 | 250 mg | – | in stock |
| 41560 | 1 g | 450.00$ | in stock |
| 61560 | 5 g | 1990.00$ | in stock |
Chemical phosphorylation reagent for the synthesis of 5’-phosphorylated oligonucleotides. The reagent contains a DMT group in its structure, which allows oligonucleotide purification on C18-cartridges or by reversed-phase chromatography. To yield 5’-phosphorylated oligonucleotide, remove DMT group and then deprotect the phosphate group with diluted ammonium solution (0.1 M). Deprotection of the phosphate group is rapid and efficient even under mild basic conditions, so this reagent can be used for DMT-Off synthesis, for example for RNA synthesis, or with dye phosphoramidites that require mild deprotection conditions.
用于合成 5′-磷酸化寡核苷酸的化学磷酸化试剂。 该试剂的结构中含有一个 DMT 基团，可以在 C18 小柱上或通过反相色谱法纯化寡核苷酸。 要产生 5′-磷酸化寡核苷酸，请去除 DMT 基团，然后用稀释的铵溶液 (0.1 M) 去保护磷酸基团。 即使在温和的碱性条件下，磷酸基团的脱保护也能快速有效地进行，因此该试剂可用于 DMT-Off 合成，例如 RNA 合成，或用于需要温和脱保护条件的染料亚磷酰胺。
Usage
Diluent: anhydrous acetonitrile. Add the diluent to the recommended concentration (0.1 M) and wait until reagent dissolution is complete while mixing periodically. This reagent is viscous amorphous, and it may take up to 10 minutes to dissolve it. Store the diluted reagent in anhydrous conditions for not more than 24 hours.
稀释剂：无水乙腈。 将稀释剂添加至推荐浓度 (0.1 M) 并等待直到试剂溶解完成，同时定期混合。 该试剂是粘性无定形的，可能需要 10 分钟才能溶解。 将稀释后的试剂在无水条件下存放不超过 24 小时。
Coupling: 6 minutes
耦合：6 分钟
Deprotection脱保护:
- DMT group is removed during synthesis: to yield the 5’-terminal phosphate, deprotect under standard conditions using ammonium hydroxide.DMT 基团在合成过程中被去除：为了产生 5′-末端磷酸盐，在标准条件下使用氢氧化铵去保护。
- DMT-ON, cartridge purification: standard cartridge purification conditions. After elution from the cartridge, deprotect the phosphate group by adding an equivalent volume of 25% aqueous ammonium solution to oligonucleotide solution, incubate for 15 minutes at room temperature, and dry the oligonucleotide down.DMT-ON，柱净化：标准柱净化条件。 从小柱中洗脱后，通过向寡核苷酸溶液中加入等体积的 25% 铵水溶液来脱去磷酸基团的保护，在室温下孵育 15 分钟，然后将寡核苷酸干燥。
- DMT-ON, purification by HPLC: purify 5’-DMT-oligonucleotide by reversed-phase HPLC. To remove DMT group, redissolve oligonucleotide in 80% acetic acid and incubate for 30 minutes at room temperature. Dry the oligonucleotide down, add 10% aqueous ammonium and incubate for 15 minutes to cleave the remaining fragment of the protecting group.DMT-ON，HPLC 纯化：通过反相 HPLC 纯化 5′-DMT-寡核苷酸。 要去除 DMT 基团，将寡核苷酸重新溶解在 80% 乙酸中并在室温下孵育 30 分钟。 将寡核苷酸干燥，加入 10% 铵水溶液并孵育 15 分钟以切割剩余的保护基片段。
- DMT-OFF: to yield the 5’-phosphate, use standard deprotection conditions after synthesis.DMT-OFF：要产生 5′-磷酸，在合成后使用标准脱保护条件。
Chemical phosphorylation reagent for oligonucleotide synthesis相关产品
TAMRA phosphoramidite, 5-isomer
TAMRA phosphoramidite for synthesis of 5’-labeled oligonucleotides. Pure 5-isomer.
TAMRA 亚磷酰胺，用于合成 5′ 标记的寡核苷酸。 纯5-异构体。
dsGreen for Real-Time PCR, 100×
Stock solution of dsGreen for Real-Time PCR, 100×
用于实时 PCR 的 dsGreen 储备溶液，100×
Alkyne Amidite, hydroxyprolinol
Acetylenic amidite for the incorporation of acetylene groups into oligos.
用于将乙炔基团结合到寡核苷酸中的乙炔酰胺。
General properties
| Appearance: | colorless semisolid |
| Molecular weight: | 722.8 |
| CAS number: | 171285-25-9 |
| Molecular formula: | C39H51N2O9P |
| IUPAC name: | Propanedioic acid, 2-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-2-[[[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]methyl]-, 1,3-diethyl ester |
| Quality control: | NMR 1H,31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | recommended coupling time 6 min |
| Deprotection conditions: | identical to protected nucleobases |
Biotin phosphoramidite (hydroxyprolinol)
Biotin phosphoramidite (hydroxyprolinol),用于合成基于羟脯氨酸核心的生物素化寡核苷酸的非核苷修饰剂。 这种亚磷酰胺含有一个二甲氧基三苯甲基保护基团，用于在 C18 小柱和反相 HPLC 上纯化所得的寡核苷酸。 该修饰剂试剂可用于将生物素引入 5′-位、3′-位（使用通用 CPG）和内部寡核苷酸位点。
- Products
- DNA
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- Biotin phosphoramidite (hydroxyprolinol)
Biotin phosphoramidite (hydroxyprolinol)
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 22360 | 250 mg | 240.00$ | in stock |
| 42360 | 1 g | 760.00$ | in stock |
| 62360 | 5 g | 3040.00$ | in stock |
| 82360 | 10 g | please inquire | in stock |
Non-nucleosidic modifier reagent for the synthesis of biotinylated oligos based on a hydroxyprolinol core. This phosphoramidite contains a dimethoxytrityl protective group for the purification of the resulting oligos on C18 cartridges, and reverse phase HPLC. This modifier reagent can be used to introduce biotin onto 5′-position, 3′-position (using universal CPG), and internal oligo sites.
This amidite does not require special condensation and deprotection conditions.
用于合成基于羟脯氨酸核心的生物素化寡核苷酸的非核苷修饰剂。 这种亚磷酰胺含有一个二甲氧基三苯甲基保护基团，用于在 C18 小柱和反相 HPLC 上纯化所得的寡核苷酸。 该修饰剂试剂可用于将生物素引入 5′-位、3′-位（使用通用 CPG）和内部寡核苷酸位点。
这种亚酰胺不需要特殊的缩合和脱保护条件。
Biotin phosphoramidite (hydroxyprolinol)相关产品
Biotin alkyne
Biotin alkyne is a reactive affinity label which can be attached to biomolecules via Click Chemistry.
生物素炔烃是一种反应性亲和标记，可以通过点击化学连接到生物分子上。
ROX azide, 5- isomer
ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.
ROX（又名罗丹明 X 或罗丹明 101）叠氮化物，用于通过 Click Chemistry 进行生物分子标记。
Alkyne Phosphoramidite, 5′-terminal
Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.
用于合成寡核苷酸的炔亚磷酰胺，可用于点击化学。
General properties
| Appearance: | white powder |
| Molecular weight: | 959.18 |
| Molecular formula: | C51H71N6O8PS |
| Solubility: | good in MeCN, DCM, THF |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing in oligo synthesis |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
储存：收到后 12 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 干燥。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
Alkyne Phosphoramidite, 5′-terminal
Alkyne Phosphoramidite, 5′-terminal,Phosphoramidite for the synthesis of oligonucleotides with 5′-terminal alkyne for Click Chemistry.
- Products
- DNA
- Phosphoramidites
- Alkyne Phosphoramidite, 5′-terminal
Alkyne Phosphoramidite, 5′-terminal
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 22260 | 250 mg | 110.00$ | in stock |
| 32260 | 500 mg | 190.00$ | in stock |
| 42260 | 1 g | 310.00$ | in stock |
| 62260 | 5 g | 1290.00$ | in stock |
| 82260 | 10 g | please inquire | in stock |
Phosphoramidite for the synthesis of oligonucleotides with 5′-terminal alkyne for Click Chemistry.
This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5′-butynyl-CEP, and other 5′-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life
Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5′-terminal DMT group, no 5′-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.
Oligonucleotides with this modification are ideal for the use in Click Chemistry (see our recommended protocol).
亚磷酰胺用于合成具有 5′-末端炔烃的寡核苷酸，用于 Click Chemistry。
与 5′-己炔基亚磷酰胺、5′-丁炔基-CEP 和其他 5′-末端炔基亚磷酰胺相比，这种炔基亚磷酰胺具有多个优点。 首先，它是更易于处理和分配的固体化合物。 并且由于其结构，在溶液中也更稳定，保质期更长
该亚磷酰胺的稀释剂是乙腈，建议偶联时间为 5 分钟。 因为这种亚酰胺不含 5′-末端 DMT 基团，所以不需要 5′-去保护。 寡核苷酸应在标准条件下解封，并通过 PAGE 或离子交换 HPLC 纯化。
具有这种修饰的寡核苷酸非常适合用于 Click Chemistry（请参阅我们推荐的方案）。
Alkyne Phosphoramidite, 5′-terminal相关产品
ROX NHS ester, 6- isomer
Pure isomer 6-ROX NHS ester for amine labeling.
用于胺标记的纯异构体 6-ROX NHS 酯。
Sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
NHS 酯形式的水溶性磺化远红色磺基-Cy5.5 染料。
Biotin phosphoramidite (hydroxyprolinol)
Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.
用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。
General properties
| Appearance: | colorless solid |
| Molecular weight: | 409.50 |
| CAS number: | 1417539-32-2 |
| Molecular formula: | C21H36N3O3P |
| IUPAC name: | trans-4-(5-Hexynoylamino)cyclohexyloxy-N,N-diisopropylamino-2-cyanoethyloxyphosphine |
| Solubility: | good in acetonitrile and dichloromethane
良好的乙腈和二氯甲烷 |
| Quality control: | NMR 1H (95%) and 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.
储存：-20°C 收货后 12 个月。 运输：在室温下长达 3 周。 干燥。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile 乙腈 |
| Coupling conditions: | Standard coupling, identical to normal nucleobases
标准偶联，与正常核碱基相同 |
| Cleavage conditions: | standard deprotection
标准脱保护 |
| Deprotection conditions: | No deprotection required. Compatible with standard deprotection reagents.
无需脱保护。 与标准脱保护试剂兼容。 |
相关文献：
- Farzan, V.M.; Kvach, M.V.; Aparin, I.O.; Kireev, D.E.; Prikazchikova, T.A.; Ustinov, A.V.; Shmanai, V.V.; Shipulin, G.A.; Korshun, V.A.; Zatsepin, T.S. Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA. Talanta, 2019, 194, 226–232. doi: 10.1016/j.talanta.2018.10.043
- Taskova, M.; Uhd, J.; Miotke, L.; Kubit, M.; Bell, J.; Ji, H.P.; Astakhova, K. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Analytical Chemistry, 2017, 89(8), 4363–4366. doi: 10.1021/acs.analchem.7b00646
- Aparin, I.O.; Farzan, V.M.; Veselova, O.A.; Chistov, A.A.; Podkolzin, A.T.; Ustinov, A.V.; Shipulin, G.A.; Formanovsky, A.A.; Korshun, V.A.; Zatsepin, T.S. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst, 2016, 141, 1331–1338. doi: 10.1039/c5an01767j
- Astakhova, I.K.; Santhosh Kumar, T.; Campbell, M.A.; Ustinov, A.V.; Korshun, V.A.; Wengel, J. Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2′-alpha-L-amino-LNA FRET pairs. Chemical Communications, 2013, 49(5), 511-511. doi: 10.1039/c2cc37547h
Alkyne Amidite, hydroxyprolinol
Alkyne Amidite, hydroxyprolinol,Phosphoramidite for the synthesis of alkyne-modified oligonucleotides. Oligonucleotides can be used for Click Chemistry modification (see the protocol).
- Products
- DNA
- Phosphoramidites
- Alkyne Amidite, hydroxyprolinol
Alkyne Amidite, hydroxyprolinol
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| 21360 | 250 mg | 110.00$ | in stock |
| 41360 | 1 g | 390.00$ | in stock |
Phosphoramidite for the synthesis of alkyne-modified oligonucleotides. Oligonucleotides can be used for Click Chemistry modification (see the protocol).
Diluent for this amidite is acetonitrile, 5 min coupling time is recommended. Standard deprotection conditions can be used for oligonucleotides.
Oligonucleotides can be purified by HPLC or cartridges due to the presence of dimethoxytrityl group, as well as by PAGE.
用于合成炔烃修饰的寡核苷酸的亚磷酰胺。 寡核苷酸可用于点击化学修饰（参见协议）。
该亚酰胺的稀释剂是乙腈，建议偶联时间为 5 分钟。 标准脱保护条件可用于寡核苷酸。
由于存在二甲氧基三苯甲基，寡核苷酸可以通过 HPLC 或小柱以及 PAGE 进行纯化。
Alkyne Amidite, hydroxyprolinol相关产品
Cyanine5.5 NHS ester
Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
Cy5.5 NHS 是远红/近红外胺活性染料。
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Alkyne derivative of ultra-bright and photostable perylene fluorophore.
超亮和光稳定苝荧光团的炔烃衍生物。
Cyanine3 NHS ester
Amine-reactive Cyanine3 dye NHS ester.
胺反应性 Cy3 染料 NHS 酯。
General properties
| Appearance: | colorless semisolid 无色半固体 |
| Molecular weight: | 713.84 |
| CAS number: | 1357289-02-1 |
| Molecular formula: | C41H52N3O6P |
| IUPAC name: | Phosphoramidous acid, N,N-bis(1-methylethyl)-, (3R,5S)-5-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-1-(1-oxo-5-hexyn-1-yl)-3-pyrrolidinyl 2-cyanoethyl ester |
| Solubility: | good in acetonitrile and dichloromethane |
| Quality control: | NMR 1H and 31P, HPLC-MS (95 %) |
| Storage conditions: | Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.
储存：-20°C 收货后 12 个月。 运输：在室温下长达 3 周。 干燥。 |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |